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The map label for this gene is tyrS [H]

Identifier: 161869380

GI number: 161869380

Start: 390823

End: 392118

Strand: Direct

Name: tyrS [H]

Synonym: NMCC_0387

Alternate gene names: 161869380

Gene position: 390823-392118 (Clockwise)

Preceding gene: 161869379

Following gene: 161869381

Centisome position: 18.15

GC content: 56.02

Gene sequence:

>1296_bases
ATGAGCGTCATCCAAGACCTGCAATCGCGCGACCTTATCGCGCAAACGACCGACATCGAAGCCTTAGACGCTTTGTTGAA
CGAACAAAAAATCGCCCTTTACTGCGGTTTCGATCCGACCGCCGACAGCCTGCACATCGGACACCTGTTGCCCGTATTGG
CATTGCGCCGCTTCCAACAGGCGGGGCATACGCCGATTGCTTTGGTGGGCGGCGCGACCGGTATGATCGGCGACCCCAGC
TTCAAAGCCGCCGAACGCAGCTTGAATTCCGCCGAAACTGTTGCCGGCTGGGTGGAAAGTATCCGCAACCAATTAACCCC
TTTCTTGAGCTTTGAAGGCGGAAATGCCGCCATTATGGCGAACAACGCCGACTGGTTCGGCAAAATGAACTGCCTCGACT
TCCTGCGCGACATCGGCAAGCATTTCTCCGTCAACGCCATGCTGAACAAAGAATCCGTCAAACAGCGCATTGACCGCGAC
GGCGCAGGCATTTCCTTCACCGAGTTCGCCTACTCCCTGCTGCAAGGCTACGACTTCGCCGAGTTGAACAAACGCTACGG
CGCGGTTTTGGAAATCGGCGGCTCCGACCAATGGGGCAACATCACCGCCGGTATCGACCTGACCCGCCGCCTGAATCAAA
AACAAGTGTTCGGCCTGACCCTGCCTTTGGTTACCAAATCAGACGGCACCAAATTCGGCAAAACCGAAGGCGGCGCAGTA
TGGCTGAACGCGAAAAAAACCTCGCCCTATCAGTTCTACCAATTCTGGCTGAAAGTCGCCGATGCCGATGTGTATAAATT
CCTGAAATACTTTACCTTCCTGTCCATCGAAGAAATCGATGCCGTCGAAGCCAAAGACCAGGCAAGCGGCAGCAAGCCCG
AAGCGCAACGCATCCTCGCCGAAGAAATGACCCGCCTGATTCACGGCGAAGAAGCCCTTGCCGCCGCGCAGCGCATTTCC
GAAAGCCTGTTTGCCGAAGACCAAAGCAGCCTGACCGAAAGCGACTTCGAGCAGCTCGCCCTCGACGGCCTGCCTGCATT
TGAAGTTTCAGACGGCATCAATGTCGTCGAAGCCTTGGTAAAAACAGGTTTGGCATCCTCCAACAAAGAAGCGCGCGGCT
TTGTGAACAGCAAAGCGGTTTTGCTCAACGGCAAACCTGCCGAAGCCAACAACCCCAACCACGCCGCCGAACGCCCCGAC
GATGCCTGCCTGCTGACCGACGAACACAAACGTTTCGGCAAATACACCATCCTTCGGCGCGGCAAACGCAACCACGCGCT
TTTGGTTTGGAAATAA

Upstream 100 bases:

>100_bases
GAAAAACAACGGTTTCTCAATACGCGCCATAACCATTGATTCGCTTAAATTTGTTACAATCGGCGGTTTGCAAAAACGCT
AATTTTTTTGAAAGAGACCG

Downstream 100 bases:

>100_bases
TCCGATTGCCGCAGAAATGCCGTCATTCCCGCGCAGGCGGGAATGACGGCGGAGGGTTGTTTGTCTCGGTTTACCTGGTT
AAAAAAGAACGATTTTCACT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSVIQDLQSRDLIAQTTDIEALDALLNEQKIALYCGFDPTADSLHIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPS
FKAAERSLNSAETVAGWVESIRNQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
GAGISFTEFAYSLLQGYDFAELNKRYGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFGLTLPLVTKSDGTKFGKTEGGAV
WLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSIEEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALAAAQRIS
ESLFAEDQSSLTESDFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNPNHAAERPD
DACLLTDEHKRFGKYTILRRGKRNHALLVWK

Sequences:

>Translated_431_residues
MSVIQDLQSRDLIAQTTDIEALDALLNEQKIALYCGFDPTADSLHIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPS
FKAAERSLNSAETVAGWVESIRNQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
GAGISFTEFAYSLLQGYDFAELNKRYGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFGLTLPLVTKSDGTKFGKTEGGAV
WLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSIEEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALAAAQRIS
ESLFAEDQSSLTESDFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNPNHAAERPD
DACLLTDEHKRFGKYTILRRGKRNHALLVWK
>Mature_430_residues
SVIQDLQSRDLIAQTTDIEALDALLNEQKIALYCGFDPTADSLHIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSF
KAAERSLNSAETVAGWVESIRNQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRDG
AGISFTEFAYSLLQGYDFAELNKRYGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFGLTLPLVTKSDGTKFGKTEGGAVW
LNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSIEEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALAAAQRISE
SLFAEDQSSLTESDFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNPNHAAERPDD
ACLLTDEHKRFGKYTILRRGKRNHALLVWK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=410, Percent_Identity=35.1219512195122, Blast_Score=247, Evalue=2e-65,
Organism=Escherichia coli, GI1787925, Length=431, Percent_Identity=62.877030162413, Blast_Score=576, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI25145802, Length=346, Percent_Identity=37.2832369942197, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI25145805, Length=227, Percent_Identity=38.3259911894273, Blast_Score=161, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6325160, Length=435, Percent_Identity=34.7126436781609, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI20130383, Length=429, Percent_Identity=36.1305361305361, Blast_Score=281, Evalue=6e-76,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47387; Mature: 47256

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIQDLQSRDLIAQTTDIEALDALLNEQKIALYCGFDPTADSLHIGHLLPVLALRRFQQ
CCHHHHHHHCCCHHCCCCHHHHHHHHCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHH
AGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIRNQLTPFLSFEGGNAAIMA
CCCCCEEEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCEEEEE
NNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRDGAGISFTEFAYSLLQGYDFA
CCCCCCCCHHHHHHHHHHHHCEEEEEEECHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHH
ELNKRYGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFGLTLPLVTKSDGTKFGKTEGGAV
HHHCCCCEEEEECCCCCCCCEEECCHHHHHCCCHHEEEEEEEEEECCCCCCCCCCCCCEE
WLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSIEEIDAVEAKDQASGSKPEAQRILA
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHH
EEMTRLIHGEEALAAAQRISESLFAEDQSSLTESDFEQLALDGLPAFEVSDGINVVEALV
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHH
KTGLASSNKEARGFVNSKAVLLNGKPAEANNPNHAAERPDDACLLTDEHKRFGKYTILRR
HHCCCCCCCHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHCCCHHHHC
GKRNHALLVWK
CCCCCEEEEEC
>Mature Secondary Structure 
SVIQDLQSRDLIAQTTDIEALDALLNEQKIALYCGFDPTADSLHIGHLLPVLALRRFQQ
CHHHHHHHCCCHHCCCCHHHHHHHHCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHH
AGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIRNQLTPFLSFEGGNAAIMA
CCCCCEEEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCEEEEE
NNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRDGAGISFTEFAYSLLQGYDFA
CCCCCCCCHHHHHHHHHHHHCEEEEEEECHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHH
ELNKRYGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFGLTLPLVTKSDGTKFGKTEGGAV
HHHCCCCEEEEECCCCCCCCEEECCHHHHHCCCHHEEEEEEEEEECCCCCCCCCCCCCEE
WLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSIEEIDAVEAKDQASGSKPEAQRILA
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHH
EEMTRLIHGEEALAAAQRISESLFAEDQSSLTESDFEQLALDGLPAFEVSDGINVVEALV
HHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHH
KTGLASSNKEARGFVNSKAVLLNGKPAEANNPNHAAERPDDACLLTDEHKRFGKYTILRR
HHCCCCCCCHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEHHHHHHCCCHHHHC
GKRNHALLVWK
CCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA