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The map label for this gene is hpaC [H]

Identifier: 161869373

GI number: 161869373

Start: 383734

End: 384234

Strand: Direct

Name: hpaC [H]

Synonym: NMCC_0380

Alternate gene names: 161869373

Gene position: 383734-384234 (Clockwise)

Preceding gene: 161869372

Following gene: 161869374

Centisome position: 17.82

GC content: 55.49

Gene sequence:

>501_bases
ATGGCGGATTTGCAGAAAACTTTTCAAACTTCGTTCCGTGATGCGATGGCATCCTGTGCGGCAGGCGTTCATGTCATCAC
GACAGACGGTGCGGCAGGGCGTTACGGCATTACAATGACGGCGGTTGCGCCGGTAACGGACGAGCCGCCGACCGTGATGC
TGTGCATCAACCGGAGTGCGCGAATCATTCCGATCCTGTCGGAAAACGGCAGCCTCTGCATCAATACGCTGGCGGACGAA
CATCAGGATGTTGCCGAACATTTTGCCGGGCTGACCGGGCTGTCGCCCGAAGAGCGGTTTGCCTACCACATCTGGCATCG
CGGCAAAACGGGACAACTTGAAATAGAGGGCGCGTTGGCGCACCTGCACGGGCATATTGTCGGCAAACATGAAATCGGCA
CGCATTTTGTGTTTTACGTCAGGCTCGACGAAATCAAAAACTGCGGGTGCAAACGCCCCGCGCTGCTGTATTTCAGACGG
CAGTTTAGATTTTTAGACTGA

Upstream 100 bases:

>100_bases
CGGAAACGTCCGACCGACGGCTTTTTGAATCAGAACTGCTGCACATGGGGGGTGCCTTGTGTGCAGCATTCTTATATAGG
GGACAGTTTAAAGGGGAAAA

Downstream 100 bases:

>100_bases
TATTCGGACAGATATATGAAAGCGATGATACTGGCGGCAGGACGCGGCGAGCGTATGCGCCCTTTGACCGATACCACTCC
GAAGCCGCTGCTCGATGTGG

Product: 4-hydroxyphenylacetate 3-hydroxylase, small subunit, putative

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-monooxygenase small component; Flavin:NADH reductase [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAPVTDEPPTVMLCINRSARIIPILSENGSLCINTLADE
HQDVAEHFAGLTGLSPEERFAYHIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
QFRFLD

Sequences:

>Translated_166_residues
MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAPVTDEPPTVMLCINRSARIIPILSENGSLCINTLADE
HQDVAEHFAGLTGLSPEERFAYHIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
QFRFLD
>Mature_165_residues
ADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAPVTDEPPTVMLCINRSARIIPILSENGSLCINTLADEH
QDVAEHFAGLTGLSPEERFAYHIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRRQ
FRFLD

Specific function: Catalyzes the reduction of free flavins (FMN, FAD and riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the large hpaB component or to other electron acceptors such as cytochrome c and Fe(3+) ion [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. HpaC subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=137, Percent_Identity=41.6058394160584, Blast_Score=114, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR011982
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.14.13.3

Molecular weight: Translated: 18421; Mature: 18290

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAPVTDEPPTVMLCINRSA
CCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEECCCC
RIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAYHIWHRGKTGQLEIEGALA
EEEEEECCCCCEEEECHHHHHHHHHHHHHCCCCCCCCHHEEEEEEECCCCCEEEEEEEEE
HLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRRQFRFLD
EECCEEEECCCCCCEEEEEEEEEHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAPVTDEPPTVMLCINRSA
CCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEECCCC
RIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAYHIWHRGKTGQLEIEGALA
EEEEEECCCCCEEEECHHHHHHHHHHHHHCCCCCCCCHHEEEEEEECCCCCEEEEEEEEE
HLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRRQFRFLD
EECCEEEECCCCCCEEEEEEEEEHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8077235; 8550403; 10633095 [H]