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The map label for this gene is mlaE [H]

Identifier: 161869245

GI number: 161869245

Start: 266972

End: 267748

Strand: Direct

Name: mlaE [H]

Synonym: NMCC_0250

Alternate gene names: 161869245

Gene position: 266972-267748 (Clockwise)

Preceding gene: 161869244

Following gene: 161869246

Centisome position: 12.4

GC content: 55.86

Gene sequence:

>777_bases
ATGAATTTTATCCGTTCCGTCGGGGCGAGAACCCTCGGCCTTATTCAATCCCTCGGCAGTATCACGCTGTTTCTGCTGAA
CATTCTGGCGAAGTCCGGTACGGCTTTCGTCCGTCCGCGCCTGAGCGTGCGCCAAGTGTATTTTGCCGGCGTGCTGTCGG
TGTTGATTGTTGCCGTTTCAGGGCTGTTTGTCGGCATGGTCTTGGGTTTGCAGGGCTATACGCAGTTGTCGAAATTCAAA
TCCGCCGATATTTTGGGCTATATGGTCGCGGCTTCGCTGTTGCGCGAACTGGGTCCGGTGTTGGCGGCGATTCTGTTTGC
CAGCAGCGCGGGCGGTGCGATGACCAGCGAAATCGGTTTGATGAAAACGACCGAACAGCTCGAAGCGATGAACGTGATGG
CGGTAAACCCCGTCGCCCGAGTGGTTGCGCCGCGCTTTTGGGCGGGCGTGTTTTCCATGCCGCTTTTGGCTTCGATTTTC
AACGTGGCGGGTATTTTCGGCGCGTATTTGGTCGGTGTAACCTGGCTGGGCTTGGACAGCGGCATTTTCTGGTCGCAAAT
GCAGAACAACATCACGATACACTACGATGTAATCAACGGTTTGATCAAATCCGCCGCGTTCGGCGTGGCGGTAACGCTGA
TTGCCGTGCATCAGGGCTTCCACTGCGTCCCGACTTCGGAAGGCATTTTGCGCGCCAGCACGCGCACGGTGGTTTCGTCC
GCCCTGACGATTTTGGCGGTCGATTTTATATTGACCGCGTGGATGTTTACAGATTGA

Upstream 100 bases:

>100_bases
TGGCATACCGCTATCCGGCGCAAACCTCATTGCATCAGGATTTGCTCGGGTAAATGAGCCGGTGCCGTCTGAAACCGTTG
AACCTTACACATAGAAATTT

Downstream 100 bases:

>100_bases
CAGGCCGTCTGAAGACGAAACGCGGAACATCGGATATTCAAGGAACTTTAATGAAAAAGAACATATTGGAATTTTGGGTC
GGACTGTTCGTCCTGATTGG

Product: putative ABC transport system permease protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNFIRSVGARTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVRQVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFK
SADILGYMVAASLLRELGPVLAAILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQGFHCVPTSEGILRASTRTVVSS
ALTILAVDFILTAWMFTD

Sequences:

>Translated_258_residues
MNFIRSVGARTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVRQVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFK
SADILGYMVAASLLRELGPVLAAILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQGFHCVPTSEGILRASTRTVVSS
ALTILAVDFILTAWMFTD
>Mature_258_residues
MNFIRSVGARTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVRQVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFK
SADILGYMVAASLLRELGPVLAAILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQGFHCVPTSEGILRASTRTVVSS
ALTILAVDFILTAWMFTD

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=258, Percent_Identity=52.3255813953488, Blast_Score=263, Evalue=8e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27428; Mature: 27428

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFIRSVGARTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVRQVYFAGVLSVLIVAVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHH
GLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLAAILFASSAGGAMTSEIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
MKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIFNVAGIFGAYLVGVTWLGLDS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQGFHCVPTSEGILRASTRTVVSS
CHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHH
ALTILAVDFILTAWMFTD
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFIRSVGARTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVRQVYFAGVLSVLIVAVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHH
GLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLAAILFASSAGGAMTSEIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
MKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIFNVAGIFGAYLVGVTWLGLDS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQGFHCVPTSEGILRASTRTVVSS
CHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHH
ALTILAVDFILTAWMFTD
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]