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The map label for this gene is rumB

Identifier: 161723317

GI number: 161723317

Start: 96137

End: 97300

Strand: Reverse

Name: rumB

Synonym: PM0070

Alternate gene names: 161723317

Gene position: 97300-96137 (Counterclockwise)

Preceding gene: 15601936

Following gene: 15601929

Centisome position: 4.31

GC content: 42.27

Gene sequence:

>1164_bases
TTGCCTTGTCAGCATTATCAACGCGGCGATTGTCGTTCTTGTCAATGGCTGGCGACGCCTTACTCAACACAGCTTGCGAA
AAAACAGCAACATCTACAACAGCAACTCCAAAAACTGGATTGTACACAAATTCAATGGCAAGCACCTTATACTTCAATAC
TGCAAGGATTTCGTAATAAAGCCAAAATGGCAGTCAGTGGGATGGTAGAACGACCGATATTAAGTCACCCACAAAGTGAG
GCGGACTTAACCGATTGCCCACTCTACCCAGCTCATTTTACAAACATCTTTACGATTTTAAGAGATTTCATTGCACGAGC
AGGCTTAGTCCCCTATAACATTCAAAAACAAAAAGGGGAACTGAAATATATTTTGCTAACAGAAAGCCAGCTTGATGGCG
GTTTAATGTTACGCTTCGTGCTCCGTTCAGAAAAAAAATTGCCTTTAGTTCAGCGCGAACTGCCCGGGTTATTAGCCAAA
TTGCCACAATTAAAAGTGGTCAGTTTAAATATTCAACCGCAACATGCTGCTATTTTAGAAGGGGAAAAAGAAATTTTTCT
CACGGAACAACACACGCTAGAAGAGTGTTTTAATCAGATTCCGTTATTCATTCGCCCACAAGGCTTTTTCCAAACCAATC
CACAAGTAGCACAAGGGCTATATGGCACTGCGCAACATTGGGTACAAGAGCTACCCGTTCACAGGCTATGGGATTTATTT
TGTGGTGTTGGAGGTTTTGGCTTACATTGTGCACAAGCCTTACAAACCCAATACCCTGATCGCATAATTCAATTAACCGG
TATTGAGATTTCCGCCTCAGCCATTGAAGCCGCAACACTCTCGGCCCAAAAATTAGGCTTAAAACAGGTAAAGTTTCAAT
CTTTAGATGCTAAACATTTCGCCTTAGCACAATCTCCCGCGGCACAAGACGACACACCCGAACTTGTGATCGTTAACCCT
CCTCGTCGCGGCATTGGTATAGAACTGGCACAATTTCTCAATCAACTCGCACCACACTTTATTTTGTATTCCAGTTGCAA
TGCAGAAACCATGGGGAAAGATGTCCTCCACTTGAGCGATTATCAACTGAAAAAAGTACAGTTATTTGATATGTTCCCAC
ATACTTCTCATTATGAAGTGCTGTGTTTACTGGAACGAAAATAA

Upstream 100 bases:

>100_bases
GGAAAATCGCAAAATATTGACCGCACTTCAACAACGCTGGCTTGATAACAAAGCCAACAAACATTAATTAATTATGTCAT
CTCCTTCTACTTCTTTTGCG

Downstream 100 bases:

>100_bases
ATGTGTTTATCTGTCTTACAAGACCGATTTTTTAAGGACATAAAAAAACGGTGGAGTCTTCCACCGTTTTTCCTTCAATA
TCATTCAACAGATTAGTCTA

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MPCQHYQRGDCRSCQWLATPYSTQLAKKQQHLQQQLQKLDCTQIQWQAPYTSILQGFRNKAKMAVSGMVERPILSHPQSE
ADLTDCPLYPAHFTNIFTILRDFIARAGLVPYNIQKQKGELKYILLTESQLDGGLMLRFVLRSEKKLPLVQRELPGLLAK
LPQLKVVSLNIQPQHAAILEGEKEIFLTEQHTLEECFNQIPLFIRPQGFFQTNPQVAQGLYGTAQHWVQELPVHRLWDLF
CGVGGFGLHCAQALQTQYPDRIIQLTGIEISASAIEAATLSAQKLGLKQVKFQSLDAKHFALAQSPAAQDDTPELVIVNP
PRRGIGIELAQFLNQLAPHFILYSSCNAETMGKDVLHLSDYQLKKVQLFDMFPHTSHYEVLCLLERK

Sequences:

>Translated_387_residues
MPCQHYQRGDCRSCQWLATPYSTQLAKKQQHLQQQLQKLDCTQIQWQAPYTSILQGFRNKAKMAVSGMVERPILSHPQSE
ADLTDCPLYPAHFTNIFTILRDFIARAGLVPYNIQKQKGELKYILLTESQLDGGLMLRFVLRSEKKLPLVQRELPGLLAK
LPQLKVVSLNIQPQHAAILEGEKEIFLTEQHTLEECFNQIPLFIRPQGFFQTNPQVAQGLYGTAQHWVQELPVHRLWDLF
CGVGGFGLHCAQALQTQYPDRIIQLTGIEISASAIEAATLSAQKLGLKQVKFQSLDAKHFALAQSPAAQDDTPELVIVNP
PRRGIGIELAQFLNQLAPHFILYSSCNAETMGKDVLHLSDYQLKKVQLFDMFPHTSHYEVLCLLERK
>Mature_386_residues
PCQHYQRGDCRSCQWLATPYSTQLAKKQQHLQQQLQKLDCTQIQWQAPYTSILQGFRNKAKMAVSGMVERPILSHPQSEA
DLTDCPLYPAHFTNIFTILRDFIARAGLVPYNIQKQKGELKYILLTESQLDGGLMLRFVLRSEKKLPLVQRELPGLLAKL
PQLKVVSLNIQPQHAAILEGEKEIFLTEQHTLEECFNQIPLFIRPQGFFQTNPQVAQGLYGTAQHWVQELPVHRLWDLFC
GVGGFGLHCAQALQTQYPDRIIQLTGIEISASAIEAATLSAQKLGLKQVKFQSLDAKHFALAQSPAAQDDTPELVIVNPP
RRGIGIELAQFLNQLAPHFILYSSCNAETMGKDVLHLSDYQLKKVQLFDMFPHTSHYEVLCLLERK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=201, Percent_Identity=30.8457711442786, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI51173878, Length=201, Percent_Identity=30.8457711442786, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1787083, Length=390, Percent_Identity=57.1794871794872, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1789148, Length=392, Percent_Identity=24.4897959183673, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24666579, Length=343, Percent_Identity=24.7813411078717, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMB_PASMU (Q9CPH1)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245007.2
- ProteinModelPortal:   Q9CPH1
- SMR:   Q9CPH1
- GeneID:   1243417
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0070
- NMPDR:   fig|272843.1.peg.70
- HOGENOM:   HBG296593
- OMA:   QPQHAAI
- ProtClustDB:   PRK03522
- BioCyc:   PMUL272843:PM0070-MONOMER
- HAMAP:   MF_01012
- InterPro:   IPR011825
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02085

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.189

Molecular weight: Translated: 43876; Mature: 43745

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 346-346 BINDING 211-211 BINDING 240-240 BINDING 269-269 BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCQHYQRGDCRSCQWLATPYSTQLAKKQQHLQQQLQKLDCTQIQWQAPYTSILQGFRNK
CCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
AKMAVSGMVERPILSHPQSEADLTDCPLYPAHFTNIFTILRDFIARAGLVPYNIQKQKGE
HHHHHHHHHHCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LKYILLTESQLDGGLMLRFVLRSEKKLPLVQRELPGLLAKLPQLKVVSLNIQPQHAAILE
EEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHEEC
GEKEIFLTEQHTLEECFNQIPLFIRPQGFFQTNPQVAQGLYGTAQHWVQELPVHRLWDLF
CCCEEEEECHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
CGVGGFGLHCAQALQTQYPDRIIQLTGIEISASAIEAATLSAQKLGLKQVKFQSLDAKHF
HCCCCHHHHHHHHHHHHCCCCEEEEECCEEEHHHHHHHHHHHHHHCHHHHHHHHCCCHHH
ALAQSPAAQDDTPELVIVNPPRRGIGIELAQFLNQLAPHFILYSSCNAETMGKDVLHLSD
HHHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHCCHHEECCC
YQLKKVQLFDMFPHTSHYEVLCLLERK
CCCCEEEEEECCCCCCCCEEEEEEECC
>Mature Secondary Structure 
PCQHYQRGDCRSCQWLATPYSTQLAKKQQHLQQQLQKLDCTQIQWQAPYTSILQGFRNK
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHH
AKMAVSGMVERPILSHPQSEADLTDCPLYPAHFTNIFTILRDFIARAGLVPYNIQKQKGE
HHHHHHHHHHCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LKYILLTESQLDGGLMLRFVLRSEKKLPLVQRELPGLLAKLPQLKVVSLNIQPQHAAILE
EEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHEEC
GEKEIFLTEQHTLEECFNQIPLFIRPQGFFQTNPQVAQGLYGTAQHWVQELPVHRLWDLF
CCCEEEEECHHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
CGVGGFGLHCAQALQTQYPDRIIQLTGIEISASAIEAATLSAQKLGLKQVKFQSLDAKHF
HCCCCHHHHHHHHHHHHCCCCEEEEECCEEEHHHHHHHHHHHHHHCHHHHHHHHCCCHHH
ALAQSPAAQDDTPELVIVNPPRRGIGIELAQFLNQLAPHFILYSSCNAETMGKDVLHLSD
HHHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHCCHHEECCC
YQLKKVQLFDMFPHTSHYEVLCLLERK
CCCCEEEEEECCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100