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The map label for this gene is pleD [H]

Identifier: 161621454

GI number: 161621454

Start: 686216

End: 687589

Strand: Reverse

Name: pleD [H]

Synonym: mll0859

Alternate gene names: 161621454

Gene position: 687589-686216 (Counterclockwise)

Preceding gene: 13471003

Following gene: 13471001

Centisome position: 9.77

GC content: 63.97

Gene sequence:

>1374_bases
ATGACCGCGCGGATCCTCGTCGTCGACGACATCCCTGCCAATGTGAGGCTGCTGGAGGTCCGGCTGCTGGCCGAATATTT
CGAGGTGCTGACCGCCACCAATGGACCCGACGCCATCGAGACCTGCGAAAACGGCAAGGTCGATGTCGTGCTGCTCGACG
TGATGATGCCCGACATGGACGGGTTCGAGGTCTGCCGCAGGCTGAAGAGCGACCCGGCGACATCGCACATTCCGGTGGTG
ATGATCACCGCGCTCGACCAGGTTTCCGACCGCGTGCGCGGGCTCGAGGCCGGCGCCGACGATTTCCTGACCAAGCCGGT
CAACGACCTGCAGCTGATGACGCGCGTCAAGAGCCTGGTCCGGTTGAAGTCTCTCACCGACGAACTCAGGCTGCGCGCCT
CGACGACGCGCAACATCGGCATCGAAGAGCTGCTCAGCCGCAATTTCGCGTCCGAGGACACGACGCCGAAAGTGCTGTTG
ATCGACGAGCGCAAATCGTCTGTCGAGCGCATCCAGAAAATGCTGCGCGACCGCGCCGACCTCGATGTTATCGGCGACCC
GCATGCCGGGTTCTTCCAGGCCGCCGAGACGTCCTATGAATGCGTGATGATCTCGACGGCCTTCGCCGATTTCGATCCGC
TGCGGCTCTGCTCGCAATTGCGCTCGCTCGACCGGACCCGTTTCGTGCCGATCATCCTTTTGGCGGAGGAGGGCGAGGAG
GAGCGCATCATCCGCGGCCTCGAACTCGGCATCAACGACTATCTGATGCGGCCGATCGACCAGCAGGAGCTGACCGCGCG
GCTGCGCACCCAGGTGCGCCGCAAGCGCTACAACGACCAATTGCGCGCCAGTGTCACCCAGACCATCGAGATGGCGGTGA
CCGACGGCCTGACCGGGCTGCACAACCGCCGCTATCTCGACAGCCATTTGCAGACGCTGTTCGATCGCGCCGTGGCGCGG
CGCCGGCCATTGTCAGTAATGATCACCGATCTCGACCGCTTCAAGTCGATCAATGACGCGCATGGCCATGATGGCGGCGA
CGAGGTGCTACGGGAATTCGCGCGGCGGCTGCGCAAGAATGTCAGGGGTATCGACCTTGCCTGCCGGTTCGGCGGCGAGG
AGTTCGTCGTGGTCATGCCGGACACCGACGGTGCCGTGGCCGAGAAAGTGGCCGAACGCATCCGCGCCGAAATCGCTCAA
AAGCCTTTCGCCATCGGCGCCGACGGCAAGACAATCGAGGTCACCGTCAGCGTCGGGGTGTCGTCGGTGCTGAAGGGCGT
TGATACGGTGGCGGCGCTGATGAAGCGCGCCGATCTCGCGCTCTACGAAGCCAAGAGCGGCGGCCGCAACCGCGTCGTAG
CCAAGGCAGCGTAA

Upstream 100 bases:

>100_bases
TATTTCGAAGCCGATCTCGGTGCCGCGTTTCATCGAAACCATCAAATCCTACCTGGGCGATGCCTGATGTCCCTTTCCAG
CCGAGCCGGAGCCTTGTGAA

Downstream 100 bases:

>100_bases
GCGTCCGCGACCGATTCCACGGTCAATCCAACAAGGTTAGCTATTTACCTTAATCCAAGCGATTCGCGAGCCTTGGTTAA
GAAACTGTTGATTTTCATAA

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTARILVVDDIPANVRLLEVRLLAEYFEVLTATNGPDAIETCENGKVDVVLLDVMMPDMDGFEVCRRLKSDPATSHIPVV
MITALDQVSDRVRGLEAGADDFLTKPVNDLQLMTRVKSLVRLKSLTDELRLRASTTRNIGIEELLSRNFASEDTTPKVLL
IDERKSSVERIQKMLRDRADLDVIGDPHAGFFQAAETSYECVMISTAFADFDPLRLCSQLRSLDRTRFVPIILLAEEGEE
ERIIRGLELGINDYLMRPIDQQELTARLRTQVRRKRYNDQLRASVTQTIEMAVTDGLTGLHNRRYLDSHLQTLFDRAVAR
RRPLSVMITDLDRFKSINDAHGHDGGDEVLREFARRLRKNVRGIDLACRFGGEEFVVVMPDTDGAVAEKVAERIRAEIAQ
KPFAIGADGKTIEVTVSVGVSSVLKGVDTVAALMKRADLALYEAKSGGRNRVVAKAA

Sequences:

>Translated_457_residues
MTARILVVDDIPANVRLLEVRLLAEYFEVLTATNGPDAIETCENGKVDVVLLDVMMPDMDGFEVCRRLKSDPATSHIPVV
MITALDQVSDRVRGLEAGADDFLTKPVNDLQLMTRVKSLVRLKSLTDELRLRASTTRNIGIEELLSRNFASEDTTPKVLL
IDERKSSVERIQKMLRDRADLDVIGDPHAGFFQAAETSYECVMISTAFADFDPLRLCSQLRSLDRTRFVPIILLAEEGEE
ERIIRGLELGINDYLMRPIDQQELTARLRTQVRRKRYNDQLRASVTQTIEMAVTDGLTGLHNRRYLDSHLQTLFDRAVAR
RRPLSVMITDLDRFKSINDAHGHDGGDEVLREFARRLRKNVRGIDLACRFGGEEFVVVMPDTDGAVAEKVAERIRAEIAQ
KPFAIGADGKTIEVTVSVGVSSVLKGVDTVAALMKRADLALYEAKSGGRNRVVAKAA
>Mature_456_residues
TARILVVDDIPANVRLLEVRLLAEYFEVLTATNGPDAIETCENGKVDVVLLDVMMPDMDGFEVCRRLKSDPATSHIPVVM
ITALDQVSDRVRGLEAGADDFLTKPVNDLQLMTRVKSLVRLKSLTDELRLRASTTRNIGIEELLSRNFASEDTTPKVLLI
DERKSSVERIQKMLRDRADLDVIGDPHAGFFQAAETSYECVMISTAFADFDPLRLCSQLRSLDRTRFVPIILLAEEGEEE
RIIRGLELGINDYLMRPIDQQELTARLRTQVRRKRYNDQLRASVTQTIEMAVTDGLTGLHNRRYLDSHLQTLFDRAVARR
RPLSVMITDLDRFKSINDAHGHDGGDEVLREFARRLRKNVRGIDLACRFGGEEFVVVMPDTDGAVAEKVAERIRAEIAQK
PFAIGADGKTIEVTVSVGVSSVLKGVDTVAALMKRADLALYEAKSGGRNRVVAKAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=235, Percent_Identity=34.8936170212766, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786584, Length=161, Percent_Identity=37.2670807453416, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=41.8181818181818, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081881, Length=181, Percent_Identity=39.7790055248619, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=39.7515527950311, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787262, Length=166, Percent_Identity=37.9518072289157, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788381, Length=161, Percent_Identity=34.1614906832298, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787541, Length=166, Percent_Identity=36.7469879518072, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=36.6459627329193, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788085, Length=167, Percent_Identity=34.1317365269461, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=36.4406779661017, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786599, Length=122, Percent_Identity=39.344262295082, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87081977, Length=184, Percent_Identity=32.0652173913043, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=36.4406779661017, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788394, Length=120, Percent_Identity=36.6666666666667, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081974, Length=159, Percent_Identity=28.3018867924528, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788956, Length=174, Percent_Identity=32.183908045977, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=40.7766990291262, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=132, Percent_Identity=34.8484848484849, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87082012, Length=121, Percent_Identity=35.5371900826446, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787229, Length=120, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=32.3809523809524, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI2367329, Length=125, Percent_Identity=38.4, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=35.5932203389831, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790552, Length=121, Percent_Identity=33.8842975206612, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790863, Length=119, Percent_Identity=29.4117647058824, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786911, Length=122, Percent_Identity=32.7868852459016, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87081921, Length=140, Percent_Identity=30.7142857142857, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788713, Length=126, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787375, Length=121, Percent_Identity=31.404958677686, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790437, Length=127, Percent_Identity=36.2204724409449, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789402, Length=95, Percent_Identity=34.7368421052632, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51062; Mature: 50931

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARILVVDDIPANVRLLEVRLLAEYFEVLTATNGPDAIETCENGKVDVVLLDVMMPDMD
CCEEEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEEEECCCCCC
GFEVCRRLKSDPATSHIPVVMITALDQVSDRVRGLEAGADDFLTKPVNDLQLMTRVKSLV
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
RLKSLTDELRLRASTTRNIGIEELLSRNFASEDTTPKVLLIDERKSSVERIQKMLRDRAD
HHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCC
LDVIGDPHAGFFQAAETSYECVMISTAFADFDPLRLCSQLRSLDRTRFVPIILLAEEGEE
CCCCCCCCCCHHHHCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCH
ERIIRGLELGINDYLMRPIDQQELTARLRTQVRRKRYNDQLRASVTQTIEMAVTDGLTGL
HHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
HNRRYLDSHLQTLFDRAVARRRPLSVMITDLDRFKSINDAHGHDGGDEVLREFARRLRKN
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
VRGIDLACRFGGEEFVVVMPDTDGAVAEKVAERIRAEIAQKPFAIGADGKTIEVTVSVGV
CCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEEECCH
SSVLKGVDTVAALMKRADLALYEAKSGGRNRVVAKAA
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEECC
>Mature Secondary Structure 
TARILVVDDIPANVRLLEVRLLAEYFEVLTATNGPDAIETCENGKVDVVLLDVMMPDMD
CEEEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEEEEEECCCCCC
GFEVCRRLKSDPATSHIPVVMITALDQVSDRVRGLEAGADDFLTKPVNDLQLMTRVKSLV
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
RLKSLTDELRLRASTTRNIGIEELLSRNFASEDTTPKVLLIDERKSSVERIQKMLRDRAD
HHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCC
LDVIGDPHAGFFQAAETSYECVMISTAFADFDPLRLCSQLRSLDRTRFVPIILLAEEGEE
CCCCCCCCCCHHHHCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCH
ERIIRGLELGINDYLMRPIDQQELTARLRTQVRRKRYNDQLRASVTQTIEMAVTDGLTGL
HHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
HNRRYLDSHLQTLFDRAVARRRPLSVMITDLDRFKSINDAHGHDGGDEVLREFARRLRKN
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
VRGIDLACRFGGEEFVVVMPDTDGAVAEKVAERIRAEIAQKPFAIGADGKTIEVTVSVGV
CCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCCCEEEEEEECCH
SSVLKGVDTVAALMKRADLALYEAKSGGRNRVVAKAA
HHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA