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The map label for this gene is lpdA [H]

Identifier: 161611270

GI number: 161611270

Start: 1112214

End: 1113614

Strand: Reverse

Name: lpdA [H]

Synonym: Reut_B4451

Alternate gene names: 161611270

Gene position: 1113614-1112214 (Counterclockwise)

Preceding gene: 73538279

Following gene: 73538277

Centisome position: 40.85

GC content: 67.81

Gene sequence:

>1401_bases
ATGAAGACCATCCAGACCGACGTCGCTGTCATCGGAGCAGGTACCGCCGGCCTCGCCGCCTACCGCGCCGCCATCGCGGC
CGGCAAGCGCGCCGTGATCATCGAAGGTGGCCCGTACGGCACCACCTGTGCCCGCGTGGGCTGCATGCCGTCCAAGCTCC
TGATTGCCGCCGCCGAAGCCGCGCATGAGTTGCGCCACACGGCCCCGTTCGGCGTACACGTGGATGGCCAGATCCGTATC
GATGGCCGCGAGGTCATGGCCCGCGTACGCAGCGAGCGCGACCGCTTCGTCGGCTTCGTGGTACGCGGCGTGGACGGCAT
TCCCGAGGCAGACCGCATTCGTGGCTACGCCTCGTTCATCGACAACACCACGCTCGAAGTAGCGGCCGACGACGGCAACA
TCACCGTGCGTGCCAGCCGCGTGGTCATCGCCACGGGGTCGCGGCCGGTGCTGCCGGCACCGTTCAAGGTGTTCGGCGAC
CGCCTGATCGTCAACGACGATGTGTTCGCATGGGAAGACCTGCCGCGCAGCGTGGCCGTGTTCGGGCCCGGCGTGATCGG
GCTGGAGCTTGGACAGGCGCTGTCGCGCCTTGGCGTGCGCGTGCGTGTGTTCGGTGTGCGCGGCTCGCTCGGTCCGCTGA
CCGACCCCGTCATACGCCGCTATGCGGACGAGACCTTCCGCAAGGCGTTCTATCTTGATACCGAGGCCAGCGTCACGGAG
ATGGCGCGCGAAGGCGATCTGGCCCGCATTACCTATATCGACCGCGAAGGCAAGTCCGTCGCGGAGACCTTCGACTACGT
GCTCGCCGCGACGGGTCGCGAGCCTAATGTGCGCGGCCTGGGCCTGGAGAACACCGGCCTGGAACTGGATGCGCGCGGCG
TGCCGGTGTTCGATCCCGTGACGCTGCAGTGCGGCGTATCGCCGGTGTTCATCGCCGGCGATGCCAACAACATCCTGCCG
CTGCTGCATGAAGCCGCCGACGAGGGCAAGGCCGCCGGCGAGAATGCCGCGACGTACCCGCGGGTGTTGCCGCTGGCCCG
CCGCGCGCCGCTCGCCGTGGTGTTCTCTGACCCGCAGATCGCTATGGTGGGCAAGCGCTTTGCCGACCTGGCCGAGGGCA
GCTTCGTCACCGGCGAAGTCAGCTTTGAAGACCAGGGCCGCAGTCGCGTCATGCTCAGGAACCGTGGGCTGATGCACGTG
TATGCCGACAAGGCGACTGGCCAGTTCCTGGGTGCCGAATGGACGGGCCCGCGCGCCGAGAACATTGCGCACCTGCTGGC
GTGGTCGTATCAGCAGGGGCTGACCATCGCGCAGATGCTGGCAATGCCGTTTTACCACCCGGTGGTGGAAGAGGGGCTGC
GCACGGCGCTGCGCGATGCAGCGGCGAAGCTGGAGGGCTGA

Upstream 100 bases:

>100_bases
CGGACCCGATGGCGCCGCGCTGCCCGCGCGGTGCCATCCAGCCCGCCGATTGATGTCCGATCGATTTCAAGAAATCTCAA
AAGGAAAGGAACCGTTCACC

Downstream 100 bases:

>100_bases
GGCGATCGGCGGCCGCCGGCCGCCGGCCGGCAGCCGGCCTACGGCCTCCGGCCAGCCGCGCCAGCGCCGTGCACTCAAAC
GTACTCAGGCGGGTTACCAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKTIQTDVAVIGAGTAGLAAYRAAIAAGKRAVIIEGGPYGTTCARVGCMPSKLLIAAAEAAHELRHTAPFGVHVDGQIRI
DGREVMARVRSERDRFVGFVVRGVDGIPEADRIRGYASFIDNTTLEVAADDGNITVRASRVVIATGSRPVLPAPFKVFGD
RLIVNDDVFAWEDLPRSVAVFGPGVIGLELGQALSRLGVRVRVFGVRGSLGPLTDPVIRRYADETFRKAFYLDTEASVTE
MAREGDLARITYIDREGKSVAETFDYVLAATGREPNVRGLGLENTGLELDARGVPVFDPVTLQCGVSPVFIAGDANNILP
LLHEAADEGKAAGENAATYPRVLPLARRAPLAVVFSDPQIAMVGKRFADLAEGSFVTGEVSFEDQGRSRVMLRNRGLMHV
YADKATGQFLGAEWTGPRAENIAHLLAWSYQQGLTIAQMLAMPFYHPVVEEGLRTALRDAAAKLEG

Sequences:

>Translated_466_residues
MKTIQTDVAVIGAGTAGLAAYRAAIAAGKRAVIIEGGPYGTTCARVGCMPSKLLIAAAEAAHELRHTAPFGVHVDGQIRI
DGREVMARVRSERDRFVGFVVRGVDGIPEADRIRGYASFIDNTTLEVAADDGNITVRASRVVIATGSRPVLPAPFKVFGD
RLIVNDDVFAWEDLPRSVAVFGPGVIGLELGQALSRLGVRVRVFGVRGSLGPLTDPVIRRYADETFRKAFYLDTEASVTE
MAREGDLARITYIDREGKSVAETFDYVLAATGREPNVRGLGLENTGLELDARGVPVFDPVTLQCGVSPVFIAGDANNILP
LLHEAADEGKAAGENAATYPRVLPLARRAPLAVVFSDPQIAMVGKRFADLAEGSFVTGEVSFEDQGRSRVMLRNRGLMHV
YADKATGQFLGAEWTGPRAENIAHLLAWSYQQGLTIAQMLAMPFYHPVVEEGLRTALRDAAAKLEG
>Mature_466_residues
MKTIQTDVAVIGAGTAGLAAYRAAIAAGKRAVIIEGGPYGTTCARVGCMPSKLLIAAAEAAHELRHTAPFGVHVDGQIRI
DGREVMARVRSERDRFVGFVVRGVDGIPEADRIRGYASFIDNTTLEVAADDGNITVRASRVVIATGSRPVLPAPFKVFGD
RLIVNDDVFAWEDLPRSVAVFGPGVIGLELGQALSRLGVRVRVFGVRGSLGPLTDPVIRRYADETFRKAFYLDTEASVTE
MAREGDLARITYIDREGKSVAETFDYVLAATGREPNVRGLGLENTGLELDARGVPVFDPVTLQCGVSPVFIAGDANNILP
LLHEAADEGKAAGENAATYPRVLPLARRAPLAVVFSDPQIAMVGKRFADLAEGSFVTGEVSFEDQGRSRVMLRNRGLMHV
YADKATGQFLGAEWTGPRAENIAHLLAWSYQQGLTIAQMLAMPFYHPVVEEGLRTALRDAAAKLEG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=27.0788912579957, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI50301238, Length=465, Percent_Identity=24.0860215053763, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI22035672, Length=285, Percent_Identity=29.8245614035088, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI291045268, Length=317, Percent_Identity=25.5520504731861, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI291045266, Length=317, Percent_Identity=25.5520504731861, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786307, Length=466, Percent_Identity=27.0386266094421, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI87082354, Length=468, Percent_Identity=26.2820512820513, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789915, Length=346, Percent_Identity=29.4797687861272, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081717, Length=459, Percent_Identity=23.7472766884532, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=27.5641025641026, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17557007, Length=482, Percent_Identity=23.6514522821577, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71983419, Length=361, Percent_Identity=28.5318559556787, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71983429, Length=361, Percent_Identity=28.5318559556787, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=26.7368421052632, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6325166, Length=325, Percent_Identity=23.6923076923077, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21358499, Length=476, Percent_Identity=28.781512605042, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24640549, Length=309, Percent_Identity=30.7443365695793, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24640551, Length=309, Percent_Identity=30.7443365695793, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24640553, Length=309, Percent_Identity=30.7443365695793, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17737741, Length=499, Percent_Identity=26.4529058116232, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50043; Mature: 50043

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIQTDVAVIGAGTAGLAAYRAAIAAGKRAVIIEGGPYGTTCARVGCMPSKLLIAAAEA
CCCEECCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCHHHHHHHHHH
AHELRHTAPFGVHVDGQIRIDGREVMARVRSERDRFVGFVVRGVDGIPEADRIRGYASFI
HHHHHHCCCCCEEECCEEEECHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHH
DNTTLEVAADDGNITVRASRVVIATGSRPVLPAPFKVFGDRLIVNDDVFAWEDLPRSVAV
CCCEEEEEECCCCEEEEEEEEEEEECCCCCCCCCHHHCCCEEEECCCCEEHHCCCCEEEE
FGPGVIGLELGQALSRLGVRVRVFGVRGSLGPLTDPVIRRYADETFRKAFYLDTEASVTE
ECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEECCCHHHHH
MAREGDLARITYIDREGKSVAETFDYVLAATGREPNVRGLGLENTGLELDARGVPVFDPV
HHHCCCEEEEEEEECCCCHHHHHHHHHHEECCCCCCCEECCCCCCCCEEECCCCCEECCE
TLQCGVSPVFIAGDANNILPLLHEAADEGKAAGENAATYPRVLPLARRAPLAVVFSDPQI
EEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCE
AMVGKRFADLAEGSFVTGEVSFEDQGRSRVMLRNRGLMHVYADKATGQFLGAEWTGPRAE
EEHHHHHHHHCCCCEEEEEEEECCCCCCEEEEECCCEEEEEECCCCCCEECCCCCCCCHH
NIAHLLAWSYQQGLTIAQMLAMPFYHPVVEEGLRTALRDAAAKLEG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTIQTDVAVIGAGTAGLAAYRAAIAAGKRAVIIEGGPYGTTCARVGCMPSKLLIAAAEA
CCCEECCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCHHHHHHHHHH
AHELRHTAPFGVHVDGQIRIDGREVMARVRSERDRFVGFVVRGVDGIPEADRIRGYASFI
HHHHHHCCCCCEEECCEEEECHHHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHH
DNTTLEVAADDGNITVRASRVVIATGSRPVLPAPFKVFGDRLIVNDDVFAWEDLPRSVAV
CCCEEEEEECCCCEEEEEEEEEEEECCCCCCCCCHHHCCCEEEECCCCEEHHCCCCEEEE
FGPGVIGLELGQALSRLGVRVRVFGVRGSLGPLTDPVIRRYADETFRKAFYLDTEASVTE
ECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEECCCHHHHH
MAREGDLARITYIDREGKSVAETFDYVLAATGREPNVRGLGLENTGLELDARGVPVFDPV
HHHCCCEEEEEEEECCCCHHHHHHHHHHEECCCCCCCEECCCCCCCCEEECCCCCEECCE
TLQCGVSPVFIAGDANNILPLLHEAADEGKAAGENAATYPRVLPLARRAPLAVVFSDPQI
EEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCE
AMVGKRFADLAEGSFVTGEVSFEDQGRSRVMLRNRGLMHVYADKATGQFLGAEWTGPRAE
EEHHHHHHHHCCCCEEEEEEEECCCCCCEEEEECCCEEEEEECCCCCCEECCCCCCCCHH
NIAHLLAWSYQQGLTIAQMLAMPFYHPVVEEGLRTALRDAAAKLEG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]