Click here to switch to the map view.

The map label for this gene is rph [H]

Identifier: 161611257

GI number: 161611257

Start: 2717488

End: 2718207

Strand: Direct

Name: rph [H]

Synonym: Reut_A2477

Alternate gene names: 161611257

Gene position: 2717488-2718207 (Clockwise)

Preceding gene: 73542162

Following gene: 73542164

Centisome position: 71.39

GC content: 69.58

Gene sequence:

>720_bases
ATGCGACCCAGCGGACGCGCAGCCGATGCGCTGCGTTCTATCAGCCTGACCCGTCACTACACCCGCCACGCCGAGGGCTC
CGTGCTGTGCGCCTTTGGCGACACCAAGGTTCTGTGCACCGCCAGCGTGCTGGCCAAGGTGCCGCCACACAAGAAGGGCA
GCGGCGAAGGCTGGGTCACGGCCGAGTACGGCATGCTGCCCCGCGCCACGCATACGCGCTCGGACCGCGAAGCCGCGCGC
GGCAAGCAGACCGGCCGCACCCAGGAAATCCAGCGCCTGATCGGCCGCGCCATGCGCTCGGTGTTCGACCTTGCCGCGCT
GGGCGAATATACGCTGCACCTCGATTGCGACGTGCTGCAGGCCGACGGCGGCACCCGCACCGCTGCCATCACCGGCGCGT
TCGTGGCCGCGCACGATGCCATCTCGACCATGCTGCGCGATGGCCTGATCGCCGCCAGCCCCATCCGCGATTTCGTCGCC
GCCGTCTCGGTCGGCATGGTCGACGGCGTGCCGCTGCTGGACCTCGACTACGCCGAAGACAGCAACTGCGATACCGACAT
GAACGTCGTCATGACCGGCAGCGGCGGTTTTGTCGAGGTGCAGGGCACGGCCGAAGGCGCCGCCTTCAGCCGCGCCGATC
TGGACGCCATGACGCGCCTGGCCGAAGACGGCATCGCGCAACTGGTCCGCCACCAGCGCCAGGCGCTGGGCCTGGCCTGA

Upstream 100 bases:

>100_bases
AGCAACCTGATGCGCTGAACGCAAACCCCGGGACGGCGGGCACGTGAGGCCCGCCGGCGTTAGAATCGCGGTCTCTACCC
GATTCGAGCGAACCATTCCC

Downstream 100 bases:

>100_bases
AGGACGGAAGTCGCCATGCAACGCCTGGTTCTGGCCTCCAACAATGCCGGCAAGCTGCGCGAATTCGGCGCGCTGCTGGC
CCCGCTCGGCTTTGACGTAG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRPSGRAADALRSISLTRHYTRHAEGSVLCAFGDTKVLCTASVLAKVPPHKKGSGEGWVTAEYGMLPRATHTRSDREAAR
GKQTGRTQEIQRLIGRAMRSVFDLAALGEYTLHLDCDVLQADGGTRTAAITGAFVAAHDAISTMLRDGLIAASPIRDFVA
AVSVGMVDGVPLLDLDYAEDSNCDTDMNVVMTGSGGFVEVQGTAEGAAFSRADLDAMTRLAEDGIAQLVRHQRQALGLA

Sequences:

>Translated_239_residues
MRPSGRAADALRSISLTRHYTRHAEGSVLCAFGDTKVLCTASVLAKVPPHKKGSGEGWVTAEYGMLPRATHTRSDREAAR
GKQTGRTQEIQRLIGRAMRSVFDLAALGEYTLHLDCDVLQADGGTRTAAITGAFVAAHDAISTMLRDGLIAASPIRDFVA
AVSVGMVDGVPLLDLDYAEDSNCDTDMNVVMTGSGGFVEVQGTAEGAAFSRADLDAMTRLAEDGIAQLVRHQRQALGLA
>Mature_239_residues
MRPSGRAADALRSISLTRHYTRHAEGSVLCAFGDTKVLCTASVLAKVPPHKKGSGEGWVTAEYGMLPRATHTRSDREAAR
GKQTGRTQEIQRLIGRAMRSVFDLAALGEYTLHLDCDVLQADGGTRTAAITGAFVAAHDAISTMLRDGLIAASPIRDFVA
AVSVGMVDGVPLLDLDYAEDSNCDTDMNVVMTGSGGFVEVQGTAEGAAFSRADLDAMTRLAEDGIAQLVRHQRQALGLA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=63.3333333333333, Blast_Score=281, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI71981632, Length=187, Percent_Identity=27.807486631016, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25316; Mature: 25316

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSGRAADALRSISLTRHYTRHAEGSVLCAFGDTKVLCTASVLAKVPPHKKGSGEGWVT
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEE
AEYGMLPRATHTRSDREAARGKQTGRTQEIQRLIGRAMRSVFDLAALGEYTLHLDCDVLQ
ECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTAAITGAFVAAHDAISTMLRDGLIAASPIRDFVAAVSVGMVDGVPLLDLDYAED
CCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCC
SNCDTDMNVVMTGSGGFVEVQGTAEGAAFSRADLDAMTRLAEDGIAQLVRHQRQALGLA
CCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPSGRAADALRSISLTRHYTRHAEGSVLCAFGDTKVLCTASVLAKVPPHKKGSGEGWVT
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEE
AEYGMLPRATHTRSDREAARGKQTGRTQEIQRLIGRAMRSVFDLAALGEYTLHLDCDVLQ
ECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTAAITGAFVAAHDAISTMLRDGLIAASPIRDFVAAVSVGMVDGVPLLDLDYAED
CCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCC
SNCDTDMNVVMTGSGGFVEVQGTAEGAAFSRADLDAMTRLAEDGIAQLVRHQRQALGLA
CCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA