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The map label for this gene is pyrB [H]

Identifier: 161525690

GI number: 161525690

Start: 2740934

End: 2741965

Strand: Reverse

Name: pyrB [H]

Synonym: Bmul_2521

Alternate gene names: 161525690

Gene position: 2741965-2740934 (Counterclockwise)

Preceding gene: 161525691

Following gene: 161525689

Centisome position: 79.51

GC content: 66.09

Gene sequence:

>1032_bases
ATGACCACCGACACCACTGGCCGCACCGGCAATCCCGCGGCGGCCGCGAGCCCCGAGCGGTTCCGCTACGGTTTCCTGAA
GGGCAACCCGCAGCTCACGAAAAACGGCGAGCTCAAGCATCTGCTGTCGATCGAGGGCCTGCCGCGCTCGATCGTCAACC
ACATTCTCGATACGGCCGAGCAGTTCGTCAGCGTGACGGACCGCGAAGTGAAGAAGGTGCCGCTGCTGCGCGGCAAGTCC
GTGTTCAACCTGTTCTTCGAGAACTCGACGCGCACGCGCACGACGTTCGAGATCGCCGCGACGCGCCTGTCGGCCGACGT
GCTGAATCTGAACATCAATGCTTCCTCGACGAGCAAGGGCGAATCGCTGCTCGACACGATCAACAACTTGTCGGCGATGC
ACGCCGACCTGTTCGTCGTGCGTCACGCATCGAGCGGCGCGCCGTACCTGATCGCCGAGCACTGCGCGCCGCACGTGCAC
GTGATCAACGCCGGCGACGGCCGTCATGCGCATCCGACGCAGGGCCTGCTCGACATGTACACGATCCGCCACTACAAGCG
CGACTTCACGAAGCTGCGCGTGGCGATCGTCGGCGACATCCTGCATTCGCGCGTCGCGCGCTCGGACATCCACGCGCTGA
CCACGCTCGGCGTGCCCGAAGTGCGCGCGATCGGCCCGCGCACGCTGCTGCCGGGCGGGCTCGAGCAGATGGGCGTGAAG
GTGTTCCACAACCTCGACGAAGGGCTGAAGGGCGTCGACGTGATCATCATGCTGCGTCTGCAGAACGAGCGGATGAGCGG
CGCGCTGCTGCCGTCCGCGCAGGAGTATTTCAAGACGTGGGGGCTCACGCCCGAGCGCCTCGCGCTCGCCGCACCCGACG
CGATCGTGATGCACCCGGGCCCGATGAACCGCGGCGTCGAGATCGACTCGCAGGTCGCCGACGGCCCGCAATCGGTGATC
CTCAATCAGGTCACGTTCGGCATTGCCGTGCGGATGGCCGTGATGGGCATCGTCGCCGGCAACAACGACTGA

Upstream 100 bases:

>100_bases
CTGAACGTCGAGGCGCAAGCCGCCTGAACGACCGCGAAACCGTATCCCGGGCCGCCGGTTCGCGCCGCGGCCCGTTTGCA
TAGTTGGAATCACGCACACC

Downstream 100 bases:

>100_bases
GCCGCTTCGCGCCTCCTTATCCAGGCATTCAACGCATTCAACGCATTCACAGACAGCGCATGAAGATTCATATCAAAGGC
GGCACGCTGATCGATCCGGT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MTTDTTGRTGNPAAAASPERFRYGFLKGNPQLTKNGELKHLLSIEGLPRSIVNHILDTAEQFVSVTDREVKKVPLLRGKS
VFNLFFENSTRTRTTFEIAATRLSADVLNLNINASSTSKGESLLDTINNLSAMHADLFVVRHASSGAPYLIAEHCAPHVH
VINAGDGRHAHPTQGLLDMYTIRHYKRDFTKLRVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQMGVK
VFHNLDEGLKGVDVIIMLRLQNERMSGALLPSAQEYFKTWGLTPERLALAAPDAIVMHPGPMNRGVEIDSQVADGPQSVI
LNQVTFGIAVRMAVMGIVAGNND

Sequences:

>Translated_343_residues
MTTDTTGRTGNPAAAASPERFRYGFLKGNPQLTKNGELKHLLSIEGLPRSIVNHILDTAEQFVSVTDREVKKVPLLRGKS
VFNLFFENSTRTRTTFEIAATRLSADVLNLNINASSTSKGESLLDTINNLSAMHADLFVVRHASSGAPYLIAEHCAPHVH
VINAGDGRHAHPTQGLLDMYTIRHYKRDFTKLRVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQMGVK
VFHNLDEGLKGVDVIIMLRLQNERMSGALLPSAQEYFKTWGLTPERLALAAPDAIVMHPGPMNRGVEIDSQVADGPQSVI
LNQVTFGIAVRMAVMGIVAGNND
>Mature_342_residues
TTDTTGRTGNPAAAASPERFRYGFLKGNPQLTKNGELKHLLSIEGLPRSIVNHILDTAEQFVSVTDREVKKVPLLRGKSV
FNLFFENSTRTRTTFEIAATRLSADVLNLNINASSTSKGESLLDTINNLSAMHADLFVVRHASSGAPYLIAEHCAPHVHV
INAGDGRHAHPTQGLLDMYTIRHYKRDFTKLRVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQMGVKV
FHNLDEGLKGVDVIIMLRLQNERMSGALLPSAQEYFKTWGLTPERLALAAPDAIVMHPGPMNRGVEIDSQVADGPQSVIL
NQVTFGIAVRMAVMGIVAGNND

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=34.9358974358974, Blast_Score=163, Evalue=2e-40,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=33.6569579288026, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI193204318, Length=333, Percent_Identity=32.1321321321321, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=313, Percent_Identity=31.629392971246, Blast_Score=132, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24642586, Length=314, Percent_Identity=34.3949044585987, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 37327; Mature: 37196

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDTTGRTGNPAAAASPERFRYGFLKGNPQLTKNGELKHLLSIEGLPRSIVNHILDTAE
CCCCCCCCCCCCCCCCCCHHHEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
QFVSVTDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAATRLSADVLNLNINASSTSKG
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHEEEEEEEEECCCCCCCH
ESLLDTINNLSAMHADLFVVRHASSGAPYLIAEHCAPHVHVINAGDGRHAHPTQGLLDMY
HHHHHHHHHHHHHHHEEEEEEECCCCCCEEECCCCCCEEEEEECCCCCCCCCCHHHHHHH
TIRHYKRDFTKLRVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQMGVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHH
VFHNLDEGLKGVDVIIMLRLQNERMSGALLPSAQEYFKTWGLTPERLALAAPDAIVMHPG
HHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCHHHEEEECCCEEEECCC
PMNRGVEIDSQVADGPQSVILNQVTFGIAVRMAVMGIVAGNND
CCCCCCEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure 
TTDTTGRTGNPAAAASPERFRYGFLKGNPQLTKNGELKHLLSIEGLPRSIVNHILDTAE
CCCCCCCCCCCCCCCCCHHHEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
QFVSVTDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAATRLSADVLNLNINASSTSKG
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHEEEEEEEEECCCCCCCH
ESLLDTINNLSAMHADLFVVRHASSGAPYLIAEHCAPHVHVINAGDGRHAHPTQGLLDMY
HHHHHHHHHHHHHHHEEEEEEECCCCCCEEECCCCCCEEEEEECCCCCCCCCCHHHHHHH
TIRHYKRDFTKLRVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPGGLEQMGVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHHHH
VFHNLDEGLKGVDVIIMLRLQNERMSGALLPSAQEYFKTWGLTPERLALAAPDAIVMHPG
HHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCHHHEEEECCCEEEECCC
PMNRGVEIDSQVADGPQSVILNQVTFGIAVRMAVMGIVAGNND
CCCCCCEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA