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The map label for this gene is alkR [H]

Identifier: 161525278

GI number: 161525278

Start: 2318159

End: 2319121

Strand: Reverse

Name: alkR [H]

Synonym: Bmul_2108

Alternate gene names: 161525278

Gene position: 2319121-2318159 (Counterclockwise)

Preceding gene: 161525279

Following gene: 161525277

Centisome position: 67.25

GC content: 70.51

Gene sequence:

>963_bases
ATGACGATCGACCTTCTCAGCGACGTGCTGACCGACCTGCGGGCCGATGCGGTCGTCACGGGGCGCTTCACGCTCAGTGC
GCCGTGGGCGATCCGCAAGCCGGCCGTCGCCGGCGCGCCGTTTCGCACCTGCGCGGGCAGCCCGTTCTACCTGTCGGTTG
CCGGCGCGGCGCCGGTGCACGTCGAGCCGGGCGACTTCGTCCTGCTGCCGCACGGCGACGAGCACGTGATGGCGTCGTCG
CTCGACGAGCCGCCCGTGCCGTTCGACACGCTGATGGCGGACAAGGGCATCCGGCCGCGCTTCGATACGCCGCTCGAATT
CGCCGCGGGCGGCGGCGGGGCGACGAGCGAGCTTTATACGGGCATCGTCGTGTATCGCGACATCGTGCGCAGCCCGCTGT
TTTCCGTGTTGCCGACGCTGATCCACGTGCGTGCGCACGACGCGGCCGTCGCGCCGTGGCTCGCGAGCACGCTGCAGAGC
TTCATCGAGGAATCGATGGCCTGTCAGCCGGGCTGGGCCGTCGCCGCGACGCGCCTCGCCGACGTATTGTTCGTGCAGCT
GCTGCGTGCGCATCTGCAGTCGTCGGCGAACCACACGGGCTGGCTGCGCGGCCTGACCGATCCGCAGATCGGCCGCGCGA
TGGCGCTGATGCACCGCGACCCGCGCCGCAACTGGCAGCTCGCGAGTCTCGCGGCCGAGGCCGGCATGTCGCGCTCGCGG
TTCTGCGCGCGCTTCGCCGAGCTGGTCGGCGAGACGCCGATCGGCCATCTGACCGCGTACCGCATGTATCTGGCGAGCGG
CGAGCTCGCGCAGGGCAGGCTGCGGCTGATCGAGATCGCGGAGCGCGTCGGCTACACGTCGGAGAAGGCGTTCGCGCGCG
CGTTTCATCGCTGGTCGGGGATGGCGCCGCGCCGCTACGCACGCAATGCACAGGATCTGTATGACCTTGCCCGGCAACGA
TGA

Upstream 100 bases:

>100_bases
CGGCCGGCGGCACGGTCGGCGCCGGCCAGAACCAGGTCGGCGTGATGGCCGGCATTCAGCAGCGCTTCTAGCCGGCCGCG
CGCACCACGGAGACCCGACG

Downstream 100 bases:

>100_bases
CGACGTCGCGCGCTATCGCGTGCCGGCGCTCGCGCGCGGGCTCGCGATCGTCGAAGCGGTGGCGGCCGCGCACGGCGGCG
CGAGCGGCGCGGCACTGGCA

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTIDLLSDVLTDLRADAVVTGRFTLSAPWAIRKPAVAGAPFRTCAGSPFYLSVAGAAPVHVEPGDFVLLPHGDEHVMASS
LDEPPVPFDTLMADKGIRPRFDTPLEFAAGGGGATSELYTGIVVYRDIVRSPLFSVLPTLIHVRAHDAAVAPWLASTLQS
FIEESMACQPGWAVAATRLADVLFVQLLRAHLQSSANHTGWLRGLTDPQIGRAMALMHRDPRRNWQLASLAAEAGMSRSR
FCARFAELVGETPIGHLTAYRMYLASGELAQGRLRLIEIAERVGYTSEKAFARAFHRWSGMAPRRYARNAQDLYDLARQR

Sequences:

>Translated_320_residues
MTIDLLSDVLTDLRADAVVTGRFTLSAPWAIRKPAVAGAPFRTCAGSPFYLSVAGAAPVHVEPGDFVLLPHGDEHVMASS
LDEPPVPFDTLMADKGIRPRFDTPLEFAAGGGGATSELYTGIVVYRDIVRSPLFSVLPTLIHVRAHDAAVAPWLASTLQS
FIEESMACQPGWAVAATRLADVLFVQLLRAHLQSSANHTGWLRGLTDPQIGRAMALMHRDPRRNWQLASLAAEAGMSRSR
FCARFAELVGETPIGHLTAYRMYLASGELAQGRLRLIEIAERVGYTSEKAFARAFHRWSGMAPRRYARNAQDLYDLARQR
>Mature_319_residues
TIDLLSDVLTDLRADAVVTGRFTLSAPWAIRKPAVAGAPFRTCAGSPFYLSVAGAAPVHVEPGDFVLLPHGDEHVMASSL
DEPPVPFDTLMADKGIRPRFDTPLEFAAGGGGATSELYTGIVVYRDIVRSPLFSVLPTLIHVRAHDAAVAPWLASTLQSF
IEESMACQPGWAVAATRLADVLFVQLLRAHLQSSANHTGWLRGLTDPQIGRAMALMHRDPRRNWQLASLAAEAGMSRSRF
CARFAELVGETPIGHLTAYRMYLASGELAQGRLRLIEIAERVGYTSEKAFARAFHRWSGMAPRRYARNAQDLYDLARQR

Specific function: This protein activates the expression of the alkane 1- monooxygenase AlkM [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786496, Length=253, Percent_Identity=24.5059288537549, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 35036; Mature: 34904

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDLLSDVLTDLRADAVVTGRFTLSAPWAIRKPAVAGAPFRTCAGSPFYLSVAGAAPVH
CCHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCHHHCCCCCEEEEECCCCCEE
VEPGDFVLLPHGDEHVMASSLDEPPVPFDTLMADKGIRPRFDTPLEFAAGGGGATSELYT
ECCCCEEEECCCCCHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHH
GIVVYRDIVRSPLFSVLPTLIHVRAHDAAVAPWLASTLQSFIEESMACQPGWAVAATRLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
DVLFVQLLRAHLQSSANHTGWLRGLTDPQIGRAMALMHRDPRRNWQLASLAAEAGMSRSR
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHH
FCARFAELVGETPIGHLTAYRMYLASGELAQGRLRLIEIAERVGYTSEKAFARAFHRWSG
HHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
MAPRRYARNAQDLYDLARQR
CCCHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
TIDLLSDVLTDLRADAVVTGRFTLSAPWAIRKPAVAGAPFRTCAGSPFYLSVAGAAPVH
CHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCCHHHCCCCCEEEEECCCCCEE
VEPGDFVLLPHGDEHVMASSLDEPPVPFDTLMADKGIRPRFDTPLEFAAGGGGATSELYT
ECCCCEEEECCCCCHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCHHHCCCCCCCHHHHHH
GIVVYRDIVRSPLFSVLPTLIHVRAHDAAVAPWLASTLQSFIEESMACQPGWAVAATRLA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
DVLFVQLLRAHLQSSANHTGWLRGLTDPQIGRAMALMHRDPRRNWQLASLAAEAGMSRSR
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHH
FCARFAELVGETPIGHLTAYRMYLASGELAQGRLRLIEIAERVGYTSEKAFARAFHRWSG
HHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
MAPRRYARNAQDLYDLARQR
CCCHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9546151 [H]