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The map label for this gene is rnhA [H]

Identifier: 161525205

GI number: 161525205

Start: 2231500

End: 2231943

Strand: Direct

Name: rnhA [H]

Synonym: Bmul_2035

Alternate gene names: 161525205

Gene position: 2231500-2231943 (Clockwise)

Preceding gene: 161525204

Following gene: 161525206

Centisome position: 64.71

GC content: 66.44

Gene sequence:

>444_bases
ATGACCACCGACACCATCGACATCTATACCGACGGCGCCTGCAAGGGCAACCCCGGCCCCGGCGGCTGGGGCGCATTGCT
GCGCTACGGCGAACGCGAGAAGGAACTGTTCGGCGGCGAGCCGAACACGACGAACAACCGGATGGAGCTGATGGGCGTGA
TCGCCGCGCTCGAGGCACTGAAGCGCCCGTGCCACGTGATCGTGCACACCGACTCGCAATACGTGCAGAAAGGCATCAGC
GAGTGGATCCACGGCTGGAAGAAAAAGGGCTGGGTCACGGCCGCGAAGACGCCGGTGAAGAACGCCGACCTGTGGAAGCG
GCTCGACGCGCTCGTCGCATTGCACGAGGTCGAGTGGCGCTGGGTCAAGGGCCATGCCGGCCATCCCGAAAACGAACGCG
CGGACGCGCTCGCGAATCGCGGCGTCGCGTCGCTCGCGGCCTGA

Upstream 100 bases:

>100_bases
TGCGCGGCATGCGGCTCGTCGGTCCGATCAAGGTGAAAAAGCCGGTGCTCGCGCCGGGCCTGACGCCGGCGGCCTCCCCG
ACCACCCATCAAGAACATTC

Downstream 100 bases:

>100_bases
GCGACGGCCGCTTTTTTGCCTTTCCCGATTTATCCGACATGCGCCAGATCATTCTCGATACCGAAACCACCGGCCTGAAC
GCCCGCTCGGGCGACCGCAT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MTTDTIDIYTDGACKGNPGPGGWGALLRYGEREKELFGGEPNTTNNRMELMGVIAALEALKRPCHVIVHTDSQYVQKGIS
EWIHGWKKKGWVTAAKTPVKNADLWKRLDALVALHEVEWRWVKGHAGHPENERADALANRGVASLAA

Sequences:

>Translated_147_residues
MTTDTIDIYTDGACKGNPGPGGWGALLRYGEREKELFGGEPNTTNNRMELMGVIAALEALKRPCHVIVHTDSQYVQKGIS
EWIHGWKKKGWVTAAKTPVKNADLWKRLDALVALHEVEWRWVKGHAGHPENERADALANRGVASLAA
>Mature_146_residues
TTDTIDIYTDGACKGNPGPGGWGALLRYGEREKELFGGEPNTTNNRMELMGVIAALEALKRPCHVIVHTDSQYVQKGISE
WIHGWKKKGWVTAAKTPVKNADLWKRLDALVALHEVEWRWVKGHAGHPENERADALANRGVASLAA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=149, Percent_Identity=35.5704697986577, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1786408, Length=133, Percent_Identity=66.1654135338346, Blast_Score=195, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI115532542, Length=142, Percent_Identity=40.8450704225352, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17505482, Length=132, Percent_Identity=34.0909090909091, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45552509, Length=146, Percent_Identity=34.2465753424658, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17137476, Length=146, Percent_Identity=34.2465753424658, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16225; Mature: 16093

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDTIDIYTDGACKGNPGPGGWGALLRYGEREKELFGGEPNTTNNRMELMGVIAALEAL
CCCCEEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KRPCHVIVHTDSQYVQKGISEWIHGWKKKGWVTAAKTPVKNADLWKRLDALVALHEVEWR
CCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCEE
WVKGHAGHPENERADALANRGVASLAA
EEECCCCCCCCHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
TTDTIDIYTDGACKGNPGPGGWGALLRYGEREKELFGGEPNTTNNRMELMGVIAALEAL
CCCEEEEEECCCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KRPCHVIVHTDSQYVQKGISEWIHGWKKKGWVTAAKTPVKNADLWKRLDALVALHEVEWR
CCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCEE
WVKGHAGHPENERADALANRGVASLAA
EEECCCCCCCCHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA