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The map label for this gene is phoB [H]

Identifier: 161525185

GI number: 161525185

Start: 2208568

End: 2209269

Strand: Reverse

Name: phoB [H]

Synonym: Bmul_2015

Alternate gene names: 161525185

Gene position: 2209269-2208568 (Counterclockwise)

Preceding gene: 161525186

Following gene: 161525184

Centisome position: 64.07

GC content: 63.82

Gene sequence:

>702_bases
ATGCCCAGCAACATTCTCGTCGTTGAAGATGAACCCGCGATTTCGGAACTGATCTCGGTGAATCTCCAGCATGCCGGTCA
CTGTCCGATTCGCGCGTACAACGCTGAACAGGCGCAGAACCTGATCAGCGACGTGCTGCCCGATCTGGTGCTGCTCGACT
GGATGCTGCCGGGCAAGTCCGGTATCGCGTTCGCGCGCGACCTGCGCAACAACGAACGGACCAAGCACATTCCGATCATC
ATGCTGACCGCGCGCGGCGACGAGCAGGACAAGGTGCTCGGCCTCGAGATCGGCGCGGACGACTACGTGACGAAGCCGTT
CTCGCCGAAGGAACTGATGGCGCGCATCAAGGCCGTGCTGCGCCGGCGCGCGCCGCAGCTGACCGAAGACGTCGTGTCGA
TCAACGGGCTGCGTCTCGATCCGGCCACGCACCGCGTCGCCGCGCAGACGAACGGCAGCGAGATCAAGCTCGATCTCGGT
CCGACCGAATTCCGGCTGCTGCATTTCTTCATGACGCATCCGGAGCGCGTGCACAGCCGCACGCAGCTGCTCGATCAGGT
GTGGGGCGATCACGTCTTCGTCGAGGAGCGCACCGTCGACGTGCATATCAAACGATTGCGCGCGGCCTTGAAACCGGCCG
GCTGCGATGCGATGATCGAGACCGTGCGCGGCAGCGGCTACCGGCTCGCGAAGCACGCGTAA

Upstream 100 bases:

>100_bases
CTACATCGTGAAGGGCACGGACGTGCGGCACCAGCCGCGCGACACGCTCGATCGCGAAGCCAACAGTTAACGCGACTACG
CCAGGAAGATAGAGGTGCCG

Downstream 100 bases:

>100_bases
CGCCATCGGGCATGCCGGCAACGCGGCATGCCGTTCATTCTTTTGGACGCTGAATCATGAACATCATCTGGGCGCGCTTT
CTGGTGTCGCTCGTGCTGCT

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MPSNILVVEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPII
MLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVSINGLRLDPATHRVAAQTNGSEIKLDLG
PTEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA

Sequences:

>Translated_233_residues
MPSNILVVEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPII
MLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVSINGLRLDPATHRVAAQTNGSEIKLDLG
PTEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA
>Mature_232_residues
PSNILVVEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKSGIAFARDLRNNERTKHIPIIM
LTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVLRRRAPQLTEDVVSINGLRLDPATHRVAAQTNGSEIKLDLGP
TEFRLLHFFMTHPERVHSRTQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=230, Percent_Identity=58.2608695652174, Blast_Score=283, Evalue=7e-78,
Organism=Escherichia coli, GI1788394, Length=233, Percent_Identity=42.0600858369099, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789809, Length=232, Percent_Identity=37.9310344827586, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1790860, Length=228, Percent_Identity=39.0350877192982, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=36.283185840708, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=34.6153846153846, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=34.9775784753363, Blast_Score=123, Evalue=9e-30,
Organism=Escherichia coli, GI1786911, Length=233, Percent_Identity=35.6223175965665, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87082012, Length=224, Percent_Identity=34.375, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=39.3162393162393, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1787229, Length=237, Percent_Identity=33.7552742616034, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789402, Length=227, Percent_Identity=33.920704845815, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1790552, Length=225, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI145693140, Length=244, Percent_Identity=31.9672131147541, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788550, Length=113, Percent_Identity=35.3982300884956, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26110; Mature: 25979

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSNILVVEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKS
CCCCEEEECCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
GIAFARDLRNNERTKHIPIIMLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVL
CHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTEDVVSINGLRLDPATHRVAAQTNGSEIKLDLGPTEFRLLHFFMTHPERVHSR
HHCCCHHHHHHHHCCCEEECCHHHEEEECCCCCEEEEECCCHHHHHHHHHHCCHHHHHHH
TQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCC
>Mature Secondary Structure 
PSNILVVEDEPAISELISVNLQHAGHCPIRAYNAEQAQNLISDVLPDLVLLDWMLPGKS
CCCEEEECCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHCCCCC
GIAFARDLRNNERTKHIPIIMLTARGDEQDKVLGLEIGADDYVTKPFSPKELMARIKAVL
CHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RRRAPQLTEDVVSINGLRLDPATHRVAAQTNGSEIKLDLGPTEFRLLHFFMTHPERVHSR
HHCCCHHHHHHHHCCCEEECCHHHEEEECCCCCEEEEECCCHHHHHHHHHHCCHHHHHHH
TQLLDQVWGDHVFVEERTVDVHIKRLRAALKPAGCDAMIETVRGSGYRLAKHA
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]