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The map label for this gene is yneJ [H]

Identifier: 161525077

GI number: 161525077

Start: 2082410

End: 2083324

Strand: Direct

Name: yneJ [H]

Synonym: Bmul_1905

Alternate gene names: 161525077

Gene position: 2082410-2083324 (Clockwise)

Preceding gene: 161525068

Following gene: 161525079

Centisome position: 60.39

GC content: 71.69

Gene sequence:

>915_bases
ATGGATCTGGCGGCGCTGGCGATTTTCCGCGCGGTCGTGCGCGAGAACGGCGTGACGCGCGCGGCGGCGAAGCTCAATCG
CGTGCAGTCGAACGTGACCACGCGCATCAAGCAGCTCGAGGAGGAACTCGGCGCGGCGCTGTTCGTGCGCGACGGCCGCC
GGCTCGTGCTCACGCCGGCCGGCCGTACGTTGCTGCCGTATGCGGAGCGGCTGCTCGCGCTGGCCGACGAAGCGCGCGAC
GCCGTGCGCGAAGACACGCCGCGCGGCCGGCTGCGGCTCGGCACGATGGAAAGCACGGCCGCGAGCCGGCTGCCGGCCGT
GCTCGCGCGCTATCACCATGCGTGGCCGGACGTTTCGCTCGAGCTCGTGACGGGCACGACGGGCTGGCTGATCGACCGCG
TGCGCGAGTTCGACGTCGACGCCGCGCTGTTCGCACGGCCGCCCGCGCCCGACACGCTGCCCGACACGTTCGAGACCGTG
CCGGTGTTCCGCGAGGAACTCGTGCTGCTCACGCCGCGCGGCCATCCGCCGGTGCGCACGCCACGCGACGTGATCCTGCC
GACGCTGATCGCATTCGAACGCGGCTGCACGTACCGCAAGTACGTCGAGCAGTGGTATGCGGAATACGGGCTGAAGCCCG
CGCGCGTGCTGGAGCTCGGCTCGTATCACGCGATCGTCGCGTGCGTCGCGGCCGGCGCCGGCATCGCGGTCGCCCCGCGC
TCGGTGCTCGACCTGCAGCCGGAAACGGACAACGTCGCGCAGCACGCGATCGCGGCATTCGGCGCGATCGATACGCTGCT
CGCGTGGCGGCACGGCTATGAATCGGCCGCCCTCGCCGCGTTGCGCGACGCGTTGACCGATGCCGCGCCGACCGCAGCCG
ACGGCGACAAACATGCGGCCACGATAGCGGCGTAA

Upstream 100 bases:

>100_bases
TACGCCGGCCGACACGGAGATCGGATGACGCCATCGTAGCTTGACCGATCATTCTCGAAAAATGAATAATTCAGAATCCA
CCCATCGCAGGGAGAGAATC

Downstream 100 bases:

>100_bases
CGCGAGCCACACTCGCCGGGCGGTTCACCGTTCATTTGCCGGGCCGCTCGCCGGGCTCCGAATGTAAAACGCCCCGACCG
GCCTGCGCCGATCGGGGCGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPAGRTLLPYAERLLALADEARD
AVREDTPRGRLRLGTMESTAASRLPAVLARYHHAWPDVSLELVTGTTGWLIDRVREFDVDAALFARPPAPDTLPDTFETV
PVFREELVLLTPRGHPPVRTPRDVILPTLIAFERGCTYRKYVEQWYAEYGLKPARVLELGSYHAIVACVAAGAGIAVAPR
SVLDLQPETDNVAQHAIAAFGAIDTLLAWRHGYESAALAALRDALTDAAPTAADGDKHAATIAA

Sequences:

>Translated_304_residues
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPAGRTLLPYAERLLALADEARD
AVREDTPRGRLRLGTMESTAASRLPAVLARYHHAWPDVSLELVTGTTGWLIDRVREFDVDAALFARPPAPDTLPDTFETV
PVFREELVLLTPRGHPPVRTPRDVILPTLIAFERGCTYRKYVEQWYAEYGLKPARVLELGSYHAIVACVAAGAGIAVAPR
SVLDLQPETDNVAQHAIAAFGAIDTLLAWRHGYESAALAALRDALTDAAPTAADGDKHAATIAA
>Mature_304_residues
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPAGRTLLPYAERLLALADEARD
AVREDTPRGRLRLGTMESTAASRLPAVLARYHHAWPDVSLELVTGTTGWLIDRVREFDVDAALFARPPAPDTLPDTFETV
PVFREELVLLTPRGHPPVRTPRDVILPTLIAFERGCTYRKYVEQWYAEYGLKPARVLELGSYHAIVACVAAGAGIAVAPR
SVLDLQPETDNVAQHAIAAFGAIDTLLAWRHGYESAALAALRDALTDAAPTAADGDKHAATIAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=282, Percent_Identity=42.9078014184397, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI87082132, Length=247, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788481, Length=296, Percent_Identity=31.7567567567568, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788296, Length=247, Percent_Identity=31.5789473684211, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=29.6875, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788887, Length=291, Percent_Identity=29.2096219931272, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787879, Length=300, Percent_Identity=28, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI157672245, Length=229, Percent_Identity=27.0742358078603, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787601, Length=172, Percent_Identity=26.7441860465116, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI145693105, Length=244, Percent_Identity=24.1803278688525, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI2367136, Length=249, Percent_Identity=28.5140562248996, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=27.1255060728745, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789204, Length=256, Percent_Identity=27.734375, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790262, Length=253, Percent_Identity=26.4822134387352, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788626, Length=149, Percent_Identity=30.8724832214765, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787530, Length=278, Percent_Identity=25.1798561151079, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994962, Length=154, Percent_Identity=31.8181818181818, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789173, Length=245, Percent_Identity=30.2040816326531, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=23.921568627451, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786401, Length=153, Percent_Identity=32.0261437908497, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33052; Mature: 33052

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCC
GRTLLPYAERLLALADEARDAVREDTPRGRLRLGTMESTAASRLPAVLARYHHAWPDVSL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEE
ELVTGTTGWLIDRVREFDVDAALFARPPAPDTLPDTFETVPVFREELVLLTPRGHPPVRT
EEEECCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCC
PRDVILPTLIAFERGCTYRKYVEQWYAEYGLKPARVLELGSYHAIVACVAAGAGIAVAPR
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHCCCCCEECCH
SVLDLQPETDNVAQHAIAAFGAIDTLLAWRHGYESAALAALRDALTDAAPTAADGDKHAA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHH
TIAA
CCCC
>Mature Secondary Structure
MDLAALAIFRAVVRENGVTRAAAKLNRVQSNVTTRIKQLEEELGAALFVRDGRRLVLTPA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCC
GRTLLPYAERLLALADEARDAVREDTPRGRLRLGTMESTAASRLPAVLARYHHAWPDVSL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEE
ELVTGTTGWLIDRVREFDVDAALFARPPAPDTLPDTFETVPVFREELVLLTPRGHPPVRT
EEEECCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCCCCCC
PRDVILPTLIAFERGCTYRKYVEQWYAEYGLKPARVLELGSYHAIVACVAAGAGIAVAPR
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHCCCCCEECCH
SVLDLQPETDNVAQHAIAAFGAIDTLLAWRHGYESAALAALRDALTDAAPTAADGDKHAA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHH
TIAA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]