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The map label for this gene is emrB [H]

Identifier: 161524925

GI number: 161524925

Start: 1938519

End: 1940078

Strand: Direct

Name: emrB [H]

Synonym: Bmul_1752

Alternate gene names: 161524925

Gene position: 1938519-1940078 (Clockwise)

Preceding gene: 161524924

Following gene: 161524935

Centisome position: 56.21

GC content: 66.41

Gene sequence:

>1560_bases
ATGGCACAGGCTCCTGTCTCTCACCCGCCCTTGCAGGGCGGGCAACTCGTGATCGGGACGATCGCGGTGTCGCTCGCGGT
CTTCATGAACGTGCTCGACACCTCGATCGCGAACGTCGCGATCCCGACGATCTCGGGCGACCTCGGCGTGTCGTCCGACC
AGGGCACGTGGGTCATCACCTCGTTCGCGGTCGCCAACGCGATCTCCGTGCCGCTGACCGGCTGGCTGACCGATCGCATC
GGGCAGGTACGCCTGTTCCTCGCGTCGATCATCCTGTTCGTCATCTCGTCGTGGATGTGCGGGCTGTCGCCGACGCTGCC
GTTCCTGCTCGCGTCGCGCGTGCTGCAGGGCGCGGTGGCCGGCCCGATGATTCCGCTGTCGCAGTCGCTGCTGCTCGCGA
GCTACCCGCGCGCGAAGGCGCCGATGGCGCTCGCGCTATGGTCGATGACGACGCTGATCGCGCCGGTCGCGGGCCCGATT
CTCGGCGGGTGGATCTCGGACAACTACTCGTGGCCGTGGATCTTCTACGTGAACATTCCGGTCGGCATCGCGGCCGCGAT
CGCGACGTGGTCGATCTATCGCACGCGCGAATCGACGGTGCGGCGCGCGCCGATCGACGGCGTCGGTCTCGCGCTGCTCG
TCCTCTGGGTCGGCTCGCTGCAGATCATGCTCGACAAGGGCAAGGACCTCGACTGGTTCGCCTCGACGACGATCATCGTG
CTCGCGCTGATCGCGATCATCTCGTTCGCGTTCTTCGTGATCTGGGAGCTGACCGCCGAGCATCCGGTCGTCGATCTGTC
GCTGTTCCGGATGCGCAACTTCTCGGGCGGCACGATCGCGCTGTCGGTCGGCTACGGGCTCTACTTCGGCAACCTCGTGC
TGTTGCCGCTCTGGCTGCAGACGCAGATCGGCTATACGGCGACGGATGCCGGCCTCGTGATGGCGCCGGTCGGGCTGTTC
GCGATCCTGCTGTCGCCGCTGACGGGCAAGTTCCTGCCGCGCACCGACCCGCGCTACATCGCGACGGCCGCGTTCCTGAT
CTTCGCGCTCTGTTTCTGGATGCGCTCGCGCTACACGACGGGCGTCGACGAATGGTCGCTGATGCTGCCGACCTTCGTGC
AGGGGATCGCGATGGCGGGCTTCTTCATCCCGCTCGTGTCGATCACGCTGTCCGGCCTGCCCGGCCACCGCATTCCGGCG
GCATCGGGCCTGTCGAACTTCGTGCGGATCATGTGCGGCGGCATCGGCACGTCGATCTTCCAGACCGCGTGGGATCACCG
CAACAACTTCCATCACGCGCAGCTGGTCGAGCAGACGAACCCCTACAACCCGACGTTCAATCAGGCCGTCACGCAGATGA
ACAACCTCGGGCTGACGCGCGACCAGGCGCACGGGCTCATCAACAACATGGCCACGCAACAGGCCGCGCAGCTCGGCGTG
AACGATCTGTTCTACATCTCGGCGGCGATCTTCGTGCTGCTGATTGCGCTGATCTGGATCACGAAGCCCGAACGCGCGGG
CGGCGGCGATGCGGGCGCGGCGGCATCGGCCGCGCATTAG

Upstream 100 bases:

>100_bases
AACGCGGGCGGCAACGCGCCGGCGCCCGCTGCGGCTGCGGCGAAGCGCACCGCCGCGCCGAAGCTGATGTAACCGTTCCG
ACTCCGGAAAGACAAACGAC

Downstream 100 bases:

>100_bases
ACGTCGCGCGCATCCGCGCAGCAAACGAAAAGCCCGGTCTCGACCGGGCTTTTTTCGTTCCGACGGCGCACGTGCGTGCG
CGCGTCATTGCGCGGTGACG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MAQAPVSHPPLQGGQLVIGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDRI
GQVRLFLASIILFVISSWMCGLSPTLPFLLASRVLQGAVAGPMIPLSQSLLLASYPRAKAPMALALWSMTTLIAPVAGPI
LGGWISDNYSWPWIFYVNIPVGIAAAIATWSIYRTRESTVRRAPIDGVGLALLVLWVGSLQIMLDKGKDLDWFASTTIIV
LALIAIISFAFFVIWELTAEHPVVDLSLFRMRNFSGGTIALSVGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGLF
AILLSPLTGKFLPRTDPRYIATAAFLIFALCFWMRSRYTTGVDEWSLMLPTFVQGIAMAGFFIPLVSITLSGLPGHRIPA
ASGLSNFVRIMCGGIGTSIFQTAWDHRNNFHHAQLVEQTNPYNPTFNQAVTQMNNLGLTRDQAHGLINNMATQQAAQLGV
NDLFYISAAIFVLLIALIWITKPERAGGGDAGAAASAAH

Sequences:

>Translated_519_residues
MAQAPVSHPPLQGGQLVIGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDRI
GQVRLFLASIILFVISSWMCGLSPTLPFLLASRVLQGAVAGPMIPLSQSLLLASYPRAKAPMALALWSMTTLIAPVAGPI
LGGWISDNYSWPWIFYVNIPVGIAAAIATWSIYRTRESTVRRAPIDGVGLALLVLWVGSLQIMLDKGKDLDWFASTTIIV
LALIAIISFAFFVIWELTAEHPVVDLSLFRMRNFSGGTIALSVGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGLF
AILLSPLTGKFLPRTDPRYIATAAFLIFALCFWMRSRYTTGVDEWSLMLPTFVQGIAMAGFFIPLVSITLSGLPGHRIPA
ASGLSNFVRIMCGGIGTSIFQTAWDHRNNFHHAQLVEQTNPYNPTFNQAVTQMNNLGLTRDQAHGLINNMATQQAAQLGV
NDLFYISAAIFVLLIALIWITKPERAGGGDAGAAASAAH
>Mature_518_residues
AQAPVSHPPLQGGQLVIGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDRIG
QVRLFLASIILFVISSWMCGLSPTLPFLLASRVLQGAVAGPMIPLSQSLLLASYPRAKAPMALALWSMTTLIAPVAGPIL
GGWISDNYSWPWIFYVNIPVGIAAAIATWSIYRTRESTVRRAPIDGVGLALLVLWVGSLQIMLDKGKDLDWFASTTIIVL
ALIAIISFAFFVIWELTAEHPVVDLSLFRMRNFSGGTIALSVGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGLFA
ILLSPLTGKFLPRTDPRYIATAAFLIFALCFWMRSRYTTGVDEWSLMLPTFVQGIAMAGFFIPLVSITLSGLPGHRIPAA
SGLSNFVRIMCGGIGTSIFQTAWDHRNNFHHAQLVEQTNPYNPTFNQAVTQMNNLGLTRDQAHGLINNMATQQAAQLGVN
DLFYISAAIFVLLIALIWITKPERAGGGDAGAAASAAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=498, Percent_Identity=54.8192771084337, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1788710, Length=510, Percent_Identity=48.2352941176471, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1790195, Length=376, Percent_Identity=32.9787234042553, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1788392, Length=402, Percent_Identity=30.0995024875622, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87081983, Length=420, Percent_Identity=24.2857142857143, Blast_Score=125, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6322958, Length=188, Percent_Identity=24.468085106383, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=172, Percent_Identity=29.6511627906977, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=133, Percent_Identity=32.3308270676692, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55749; Mature: 55618

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQAPVSHPPLQGGQLVIGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVIT
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEE
SFAVANAISVPLTGWLTDRIGQVRLFLASIILFVISSWMCGLSPTLPFLLASRVLQGAVA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
GPMIPLSQSLLLASYPRAKAPMALALWSMTTLIAPVAGPILGGWISDNYSWPWIFYVNIP
CCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
VGIAAAIATWSIYRTRESTVRRAPIDGVGLALLVLWVGSLQIMLDKGKDLDWFASTTIIV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
LALIAIISFAFFVIWELTAEHPVVDLSLFRMRNFSGGTIALSVGYGLYFGNLVLLPLWLQ
HHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHH
TQIGYTATDAGLVMAPVGLFAILLSPLTGKFLPRTDPRYIATAAFLIFALCFWMRSRYTT
HHCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GVDEWSLMLPTFVQGIAMAGFFIPLVSITLSGLPGHRIPAASGLSNFVRIMCGGIGTSIF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QTAWDHRNNFHHAQLVEQTNPYNPTFNQAVTQMNNLGLTRDQAHGLINNMATQQAAQLGV
HHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
NDLFYISAAIFVLLIALIWITKPERAGGGDAGAAASAAH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AQAPVSHPPLQGGQLVIGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVIT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEE
SFAVANAISVPLTGWLTDRIGQVRLFLASIILFVISSWMCGLSPTLPFLLASRVLQGAVA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
GPMIPLSQSLLLASYPRAKAPMALALWSMTTLIAPVAGPILGGWISDNYSWPWIFYVNIP
CCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
VGIAAAIATWSIYRTRESTVRRAPIDGVGLALLVLWVGSLQIMLDKGKDLDWFASTTIIV
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
LALIAIISFAFFVIWELTAEHPVVDLSLFRMRNFSGGTIALSVGYGLYFGNLVLLPLWLQ
HHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHH
TQIGYTATDAGLVMAPVGLFAILLSPLTGKFLPRTDPRYIATAAFLIFALCFWMRSRYTT
HHCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
GVDEWSLMLPTFVQGIAMAGFFIPLVSITLSGLPGHRIPAASGLSNFVRIMCGGIGTSIF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QTAWDHRNNFHHAQLVEQTNPYNPTFNQAVTQMNNLGLTRDQAHGLINNMATQQAAQLGV
HHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
NDLFYISAAIFVLLIALIWITKPERAGGGDAGAAASAAH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]