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The map label for this gene is rlmN [H]

Identifier: 161524634

GI number: 161524634

Start: 1606281

End: 1607417

Strand: Direct

Name: rlmN [H]

Synonym: Bmul_1461

Alternate gene names: 161524634

Gene position: 1606281-1607417 (Clockwise)

Preceding gene: 161524633

Following gene: 161524635

Centisome position: 46.58

GC content: 65.17

Gene sequence:

>1137_bases
ATGACGAGCGAAACTTCCGTCAATCTTCTCGACTTCGACGCCGAGGGTCTTGTCGCGTACTGCGGCAGCCTCGGCGAGAA
GCCGTTCCGCGCCAAGCAGTTGCAGCGCTGGATCCACCAGTACAACGCCGGCAATTTCGACGGCATGACCGATCTCGCGA
AGTCCCTCAGGGAAAAGCTGAAGGGGCGCGCGTCGATCACGATGCCCGACATCGTCAGCGATCACGTTTCCGCCGACGGC
ACGCGCAAGTGGCTCGTCGACGTCGGCAACGGCAACGCGGTCGAAACCGTGTTCATCCCGGAGGAAACGCGCGGCACGCT
CTGCGTGTCGTCGCAGGCCGGGTGCGCGGTCAACTGCCGCTTCTGTTCGACGGGCAAGCAGGGGTTTTCGCGCAACCTGT
CGACGGCCGAGATCATCGGCCAGTTGCGCATGGCCGAATTTGCGCTGCGTGCGTCGCTCGGGCGCGCGCCGGGGCCGAAC
GGCAAGGCCGAGCGCGTCGTTACGAACGTCGTGATGATGGGCATGGGCGAGCCGCTCCTCAATTACAACGCGGTCGTTCC
CGCGATGCGGCTGATGCTCGACGACAACGCGTACGGGCTGTCGCGCCGTCGCGTCACGCTGTCGACCTCGGGCGTGGTGC
CGATGATGGACCGTCTCGGCGCCGAGCTGCCGGTCGCGCTCGCGGTGTCGCTGCATGCGCCGAACGATGCGCTGCGCGAC
GAACTCGTGCCGCTGAACAAGAAGCATCCGCTGCGCGAGCTGATGGCTGCGTGCCAGCGTTATCTGAAGGTCGCGCCGCG
CGACTTCATTACTTTCGAATACTGCATGCTCGACGGTGTCAACGACACCGAAGCGCATGCGCGCGAACTGCTGGCCGTCA
CGCGCGACGTGCCGTGCAAGTTCAATCTGATCCCGTTCAACCCGTTCCCGGAGTCGGGCCTCATCCGCTCGAAGCCGGAG
CAGATCAAGCGTTTCGCGCAGATCCTGATCGACGCGGGCGTCGTGACGACCGTGCGCAAGACGCGCGGCGACGACATCGA
CGCCGCATGCGGTCAGCTCGCCGGTGCAGTGAAGGACCGCACGCGCCTGGCGGAACGAACGGGCGCAGGAAAAATCATCG
AGGTTCGGGCGGTTTGA

Upstream 100 bases:

>100_bases
GAAATCGCGTTCTTCTTCCCGGAAATGAACGTTTACTCGCGTTAAGCCCTGGCTTCTAGCAAGTAGGCAGGATTCAGCGC
ACGCGGCAAGGCAGCACATC

Downstream 100 bases:

>100_bases
CGGGGTGACCCGGCCGGGGATGCCCGAACCCGTAGCGGTACAACACGCGAATAGCGATCGTCGCGGCGCACGGCGCCGCG
CATGGCATGAACAGTTGCGG

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTSETSVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGNFDGMTDLAKSLREKLKGRASITMPDIVSDHVSADG
TRKWLVDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPN
GKAERVVTNVVMMGMGEPLLNYNAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
ELVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCKFNLIPFNPFPESGLIRSKPE
QIKRFAQILIDAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGAGKIIEVRAV

Sequences:

>Translated_378_residues
MTSETSVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGNFDGMTDLAKSLREKLKGRASITMPDIVSDHVSADG
TRKWLVDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPN
GKAERVVTNVVMMGMGEPLLNYNAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
ELVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCKFNLIPFNPFPESGLIRSKPE
QIKRFAQILIDAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGAGKIIEVRAV
>Mature_377_residues
TSETSVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGNFDGMTDLAKSLREKLKGRASITMPDIVSDHVSADGT
RKWLVDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPNG
KAERVVTNVVMMGMGEPLLNYNAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRDE
LVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCKFNLIPFNPFPESGLIRSKPEQ
IKRFAQILIDAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLAERTGAGKIIEVRAV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=380, Percent_Identity=53.9473684210526, Blast_Score=402, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004383 [H]

Pfam domain/function: NA

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41267; Mature: 41136

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSETSVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGNFDGMTDLAKSLREKL
CCCCCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRASITMPDIVSDHVSADGTRKWLVDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCR
CCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPNGKAERVVTNVVMMGMGEPLL
EECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHH
NYNAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
CCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCHHHHH
ELVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCK
HCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEHEECCCCCHHHHHHHHHHHHCCCCCE
FNLIPFNPFPESGLIRSKPEQIKRFAQILIDAGVVTTVRKTRGDDIDAACGQLAGAVKDR
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
TRLAERTGAGKIIEVRAV
HHHHHHCCCCCEEEEEEC
>Mature Secondary Structure 
TSETSVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAGNFDGMTDLAKSLREKL
CCCCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRASITMPDIVSDHVSADGTRKWLVDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCR
CCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLSTAEIIGQLRMAEFALRASLGRAPGPNGKAERVVTNVVMMGMGEPLL
EECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHH
NYNAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
CCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCHHHHH
ELVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCK
HCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEHEECCCCCHHHHHHHHHHHHCCCCCE
FNLIPFNPFPESGLIRSKPEQIKRFAQILIDAGVVTTVRKTRGDDIDAACGQLAGAVKDR
EEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHH
TRLAERTGAGKIIEVRAV
HHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA