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The map label for this gene is rumA [H]

Identifier: 161524629

GI number: 161524629

Start: 1601862

End: 1603325

Strand: Direct

Name: rumA [H]

Synonym: Bmul_1456

Alternate gene names: 161524629

Gene position: 1601862-1603325 (Clockwise)

Preceding gene: 161524627

Following gene: 161524630

Centisome position: 46.45

GC content: 66.94

Gene sequence:

>1464_bases
ATGCCGCTTTCTGCTGCTAGCTTCGTCTACAATCTCGCCTTCTTCAACGTTTGTCAGGAAAAGCTGGTGTCTGAAGCCGT
CCCCACTTCTGCGCGCAAATTGAAAAGCGTGCCCGCCGCGCCCGGGAGCGCCCCGGTTCTCGAGATCGAGTCGCTCGACA
TGGAAGCGCGCGGTGTCGGCCGTGCGACGACCGAGGACGGCGAGCCGGGCAAGGTAATCTTCGTCGAGGGCGCGCTGCCC
GGCGAGCGCGTGACCTATTCGAGCTACCGCCGCAAGCCCAGCTACGAGCAGGCGACGGTCGTCGACATCCTGCGACCGAG
CGTGATGCGTACGCAGCCGAAGTGCGCGTTCTTCGGCACCTGCGGCGGCTGCTCGATGCAGCATCTCGACATGCGCGCAC
AGGTCGCGATCAAGCAGCGCGTGCTCGAGGACAATCTCTGGCATCTCGCGAAGCTGCGCGCGGAGTCGATCTTCGCGCCG
ATCCACGGCCCGTCGTGGGGCTATCGCTATCGCGCGCGCCTGACCGTGCGCCATGTGGCGAAGAAGGGCGGCGTGCTGGT
CGGCTTCCACGAGAAGAAGAGCAGCTACGTGGCCGACATGACGAGCTGCGAGGTGCTGCCGCCGCACGTATCGGCGATGC
TCGTGCCGCTGCGCCGGCTCGTCGAGGGGCTGTCCATTCGCGACCGGATGCCGCAGATCGAGCTCGCGGTCGGCTCGCAG
GTGACGGCGCTCGTGCTGCGCGTGCTCGAGCCGATCAACGCGAACGACGAAGCGCTGCTGCGCGCGTTTGCGGACGAACA
CAAGGTGCAGTTCTGGCTGCAGCCGAAGGGGCCGGACACGGTCACGCCGTTCTATCCGCTCGACGTGTCGCTCGACTACA
CGCTGCCGGAATTCGGCATCCGGATGCCGTTCAAGCCGACCGACTTCACGCAGGTGAACCATCAGATCAACCGCGTGCTG
GTCGGCCGCGCGCTGCGTCTGCTCGCGCCGGCGCGCGACGATCGCGTGCTCGATCTGTTCTGCGGGATCGGCAACTTCAC
GCTGCCGCTTGCGCGGCTCGCGCGCGAGGTGGTGGGCATCGAGGGCAGCGAGACGCTGACCACGCGCGCGCTCGCCAACG
CACGCGAGAACGGCGTCGACGGTCATACGACGTTCGCGTGCCGCAACCTGTTCGAGGTGACGGCCGACGACATCCGCGCG
CTCGGCGCCTTCGACAAGTTCCTGATCGATCCGCCGCGCGAAGGCGCGCTCGCGGTGTCGAAGGCGCTGGCCGACATCGC
GCAGAGCGGCGAGGGTCCGCTGCCGAAGCGGATCGTCTACGTGTCGTGCAATCCGTCGACGCTCGCGCGCGACGCAGGCC
TGCTCGTGCATGAAGCCGGCTACCGGCTCAAGGGCGCCGGCGTTGTCAACATGTTCCCGAATACGTCGCACGTCGAATCG
ATCGCGCTGTTCGAGCGCGACTGA

Upstream 100 bases:

>100_bases
CCGCGGCGATCGTGCGGCGCAAGTCGACGGCGCCGTCGGCGTCCCGACCCCATTGGATGTTCCAGTCGATCATGCGCATG
ATGGAATCTCCCCGCGGCGG

Downstream 100 bases:

>100_bases
GCGACGCAGGCCGGGCGGCAGCGGACGTAAAAAAACCGGCCCGTGATGAACTGCACCCCAAAAGTTGGACTAGATCCAAC
CTTTGGGGTGTTTTTCATGA

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MPLSAASFVYNLAFFNVCQEKLVSEAVPTSARKLKSVPAAPGSAPVLEIESLDMEARGVGRATTEDGEPGKVIFVEGALP
GERVTYSSYRRKPSYEQATVVDILRPSVMRTQPKCAFFGTCGGCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAESIFAP
IHGPSWGYRYRARLTVRHVAKKGGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSQ
VTALVLRVLEPINANDEALLRAFADEHKVQFWLQPKGPDTVTPFYPLDVSLDYTLPEFGIRMPFKPTDFTQVNHQINRVL
VGRALRLLAPARDDRVLDLFCGIGNFTLPLARLAREVVGIEGSETLTTRALANARENGVDGHTTFACRNLFEVTADDIRA
LGAFDKFLIDPPREGALAVSKALADIAQSGEGPLPKRIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVES
IALFERD

Sequences:

>Translated_487_residues
MPLSAASFVYNLAFFNVCQEKLVSEAVPTSARKLKSVPAAPGSAPVLEIESLDMEARGVGRATTEDGEPGKVIFVEGALP
GERVTYSSYRRKPSYEQATVVDILRPSVMRTQPKCAFFGTCGGCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAESIFAP
IHGPSWGYRYRARLTVRHVAKKGGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSQ
VTALVLRVLEPINANDEALLRAFADEHKVQFWLQPKGPDTVTPFYPLDVSLDYTLPEFGIRMPFKPTDFTQVNHQINRVL
VGRALRLLAPARDDRVLDLFCGIGNFTLPLARLAREVVGIEGSETLTTRALANARENGVDGHTTFACRNLFEVTADDIRA
LGAFDKFLIDPPREGALAVSKALADIAQSGEGPLPKRIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVES
IALFERD
>Mature_486_residues
PLSAASFVYNLAFFNVCQEKLVSEAVPTSARKLKSVPAAPGSAPVLEIESLDMEARGVGRATTEDGEPGKVIFVEGALPG
ERVTYSSYRRKPSYEQATVVDILRPSVMRTQPKCAFFGTCGGCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAESIFAPI
HGPSWGYRYRARLTVRHVAKKGGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSQV
TALVLRVLEPINANDEALLRAFADEHKVQFWLQPKGPDTVTPFYPLDVSLDYTLPEFGIRMPFKPTDFTQVNHQINRVLV
GRALRLLAPARDDRVLDLFCGIGNFTLPLARLAREVVGIEGSETLTTRALANARENGVDGHTTFACRNLFEVTADDIRAL
GAFDKFLIDPPREGALAVSKALADIAQSGEGPLPKRIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVESI
ALFERD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=200, Percent_Identity=30, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI51173878, Length=200, Percent_Identity=30, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789148, Length=446, Percent_Identity=36.5470852017937, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1787083, Length=402, Percent_Identity=25.1243781094527, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790403, Length=359, Percent_Identity=22.8412256267409, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24666579, Length=228, Percent_Identity=30.7017543859649, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 53472; Mature: 53341

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSAASFVYNLAFFNVCQEKLVSEAVPTSARKLKSVPAAPGSAPVLEIESLDMEARGVG
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEEECCCCHHCCCC
RATTEDGEPGKVIFVEGALPGERVTYSSYRRKPSYEQATVVDILRPSVMRTQPKCAFFGT
CCCCCCCCCCEEEEEECCCCCCCEEHHHHCCCCCCCHHHHHHHHCCHHHCCCCCEEEEEC
CGGCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAESIFAPIHGPSWGYRYRARLTVRHVA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEEHHHHHH
KKGGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSQ
HHCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCH
VTALVLRVLEPINANDEALLRAFADEHKVQFWLQPKGPDTVTPFYPLDVSLDYTLPEFGI
HHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEEEECCCHHHCC
RMPFKPTDFTQVNHQINRVLVGRALRLLAPARDDRVLDLFCGIGNFTLPLARLAREVVGI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCC
EGSETLTTRALANARENGVDGHTTFACRNLFEVTADDIRALGAFDKFLIDPPREGALAVS
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHH
KALADIAQSGEGPLPKRIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVES
HHHHHHHHCCCCCCCCEEEEEECCCHHHHHHCCEEEECCCCEEECCCEEEECCCCCCHHE
IALFERD
EEEEECC
>Mature Secondary Structure 
PLSAASFVYNLAFFNVCQEKLVSEAVPTSARKLKSVPAAPGSAPVLEIESLDMEARGVG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEEECCCCHHCCCC
RATTEDGEPGKVIFVEGALPGERVTYSSYRRKPSYEQATVVDILRPSVMRTQPKCAFFGT
CCCCCCCCCCEEEEEECCCCCCCEEHHHHCCCCCCCHHHHHHHHCCHHHCCCCCEEEEEC
CGGCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAESIFAPIHGPSWGYRYRARLTVRHVA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEEHHHHHH
KKGGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEGLSIRDRMPQIELAVGSQ
HHCCEEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCH
VTALVLRVLEPINANDEALLRAFADEHKVQFWLQPKGPDTVTPFYPLDVSLDYTLPEFGI
HHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEEEEECCCHHHCC
RMPFKPTDFTQVNHQINRVLVGRALRLLAPARDDRVLDLFCGIGNFTLPLARLAREVVGI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCC
EGSETLTTRALANARENGVDGHTTFACRNLFEVTADDIRALGAFDKFLIDPPREGALAVS
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHH
KALADIAQSGEGPLPKRIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPNTSHVES
HHHHHHHHCCCCCCCCEEEEEECCCHHHHHHCCEEEECCCCEEECCCEEEECCCCCCHHE
IALFERD
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA