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The map label for this gene is holB [H]

Identifier: 161524555

GI number: 161524555

Start: 1525505

End: 1526533

Strand: Direct

Name: holB [H]

Synonym: Bmul_1382

Alternate gene names: 161524555

Gene position: 1525505-1526533 (Clockwise)

Preceding gene: 161524554

Following gene: 161524556

Centisome position: 44.24

GC content: 71.72

Gene sequence:

>1029_bases
ATGATCTATCCGTGGCAGACCGACGACTGGAACCGCCTGCAGCAGTTGCGCGCGCAATGGCCGCATGCGCTGCTGCTGCA
CGGGCAGGCCGGCATCGGCAAGCTGCAGTTCGCGCAGCATCTGGCGCGGGGTTTCCTCTGCGAGTCGCCGCAGCCGAACG
GCGAGCCGTGCGGCACCTGCGCGGCCTGCACGTGGTTCGCGCAGGGCAACCATCCGGACTACCGGATCGTGCTGCCCGAG
GCGCTCGCCGGCGAAGCGCCGGGCGCCGCGGACGATGCGAAGCCGGCCGACGCGGACGACGGCGGCAAGAAGACGCGCGC
GCCGAGCAAGGAGATCAAGATCGAGCAGGTGCGTGCGCTGCTCGATTTCTGCGGCGTCGGCTCGCATCGCGGCGGCCTGC
GCGTCGTCGTGCTGTATCCGGCCGAGGCGCTGAACGTCGCCGCGTCGAACGCGTTGCTGAAGACGCTCGAGGAGCCGCCG
CCGGGCGTCGTGTTCCTGCTCGTGTCGGCGCGCATCGACCGTCTGCTGCCGACCATCGTCAGCCGCTGCCGGCAGTGGCC
GATGAGCGTGCCGGCGCCCGAGGCCGCCGCCGGTTGGCTCGCGACGCAGGGCGTCGACGACGCGCGCGCGCTGCTCGCCG
AAGCCGGCGGCGCGCCGCTCGCCGCGCTGGCGCTCGCGAGCGACGAGAACCGCCCGCTACGCGACTTCACGCTGAACCAG
CTCGCGGCCGGCGCGGCCTGCGATCCGTTCGCCTGCGGCGAGACGCTGCAGAAGCTGCCGGTGCCGCTCGTGCTCGGCTG
GCTGCAGCGCTGGCTGTACGATCTGCTGGCGCAGCGGATGGCGGGCGCGCCGCGCTATTTCCCCGCGCACGCGGCGGCGC
TCGCGCGCTGCGCGCAGACGGTCGACGCGAACGCGTTCGCGCGCTTCATGAAGGCCGTCACGCGGCAGCGCACGGTCGAG
AACCATCCGCTCAATGCGCGGCTCGTCTTCGAGGAATTGTTTCTCGGTTATCGCGAAATGTTCGCATGA

Upstream 100 bases:

>100_bases
CATCGCTTCGCGATCGTCGACGCGACGCAGTCGATCGACGAGATCCGTCAGCAGCTCGAGCGCGTGCTCGCCGCGCTGTA
ACCCGCAAGGAACGCACCTC

Downstream 100 bases:

>100_bases
TCAACGATTGTTTCCGTCGGGTTGCGAAACGATGACCGACACCTGCCGCGAGGCGACGCGCGCGTCGCGGCCGGCTTTAC
GTATTCAGGAACCACGATGT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MIYPWQTDDWNRLQQLRAQWPHALLLHGQAGIGKLQFAQHLARGFLCESPQPNGEPCGTCAACTWFAQGNHPDYRIVLPE
ALAGEAPGAADDAKPADADDGGKKTRAPSKEIKIEQVRALLDFCGVGSHRGGLRVVVLYPAEALNVAASNALLKTLEEPP
PGVVFLLVSARIDRLLPTIVSRCRQWPMSVPAPEAAAGWLATQGVDDARALLAEAGGAPLAALALASDENRPLRDFTLNQ
LAAGAACDPFACGETLQKLPVPLVLGWLQRWLYDLLAQRMAGAPRYFPAHAAALARCAQTVDANAFARFMKAVTRQRTVE
NHPLNARLVFEELFLGYREMFA

Sequences:

>Translated_342_residues
MIYPWQTDDWNRLQQLRAQWPHALLLHGQAGIGKLQFAQHLARGFLCESPQPNGEPCGTCAACTWFAQGNHPDYRIVLPE
ALAGEAPGAADDAKPADADDGGKKTRAPSKEIKIEQVRALLDFCGVGSHRGGLRVVVLYPAEALNVAASNALLKTLEEPP
PGVVFLLVSARIDRLLPTIVSRCRQWPMSVPAPEAAAGWLATQGVDDARALLAEAGGAPLAALALASDENRPLRDFTLNQ
LAAGAACDPFACGETLQKLPVPLVLGWLQRWLYDLLAQRMAGAPRYFPAHAAALARCAQTVDANAFARFMKAVTRQRTVE
NHPLNARLVFEELFLGYREMFA
>Mature_342_residues
MIYPWQTDDWNRLQQLRAQWPHALLLHGQAGIGKLQFAQHLARGFLCESPQPNGEPCGTCAACTWFAQGNHPDYRIVLPE
ALAGEAPGAADDAKPADADDGGKKTRAPSKEIKIEQVRALLDFCGVGSHRGGLRVVVLYPAEALNVAASNALLKTLEEPP
PGVVFLLVSARIDRLLPTIVSRCRQWPMSVPAPEAAAGWLATQGVDDARALLAEAGGAPLAALALASDENRPLRDFTLNQ
LAAGAACDPFACGETLQKLPVPLVLGWLQRWLYDLLAQRMAGAPRYFPAHAAALARCAQTVDANAFARFMKAVTRQRTVE
NHPLNARLVFEELFLGYREMFA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=289, Percent_Identity=32.1799307958478, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1786676, Length=165, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37084; Mature: 37084

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYPWQTDDWNRLQQLRAQWPHALLLHGQAGIGKLQFAQHLARGFLCESPQPNGEPCGTC
CCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHH
AACTWFAQGNHPDYRIVLPEALAGEAPGAADDAKPADADDGGKKTRAPSKEIKIEQVRAL
HHHHHHCCCCCCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LDFCGVGSHRGGLRVVVLYPAEALNVAASNALLKTLEEPPPGVVFLLVSARIDRLLPTIV
HHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHH
SRCRQWPMSVPAPEAAAGWLATQGVDDARALLAEAGGAPLAALALASDENRPLRDFTLNQ
HHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHEEECCCCCCHHHHHHHH
LAAGAACDPFACGETLQKLPVPLVLGWLQRWLYDLLAQRMAGAPRYFPAHAAALARCAQT
HHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VDANAFARFMKAVTRQRTVENHPLNARLVFEELFLGYREMFA
HCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYPWQTDDWNRLQQLRAQWPHALLLHGQAGIGKLQFAQHLARGFLCESPQPNGEPCGTC
CCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHH
AACTWFAQGNHPDYRIVLPEALAGEAPGAADDAKPADADDGGKKTRAPSKEIKIEQVRAL
HHHHHHCCCCCCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LDFCGVGSHRGGLRVVVLYPAEALNVAASNALLKTLEEPPPGVVFLLVSARIDRLLPTIV
HHHHCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHH
SRCRQWPMSVPAPEAAAGWLATQGVDDARALLAEAGGAPLAALALASDENRPLRDFTLNQ
HHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHEEECCCCCCHHHHHHHH
LAAGAACDPFACGETLQKLPVPLVLGWLQRWLYDLLAQRMAGAPRYFPAHAAALARCAQT
HHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VDANAFARFMKAVTRQRTVENHPLNARLVFEELFLGYREMFA
HCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]