Click here to switch to the map view.

The map label for this gene is dgoD [H]

Identifier: 161523811

GI number: 161523811

Start: 691375

End: 692523

Strand: Reverse

Name: dgoD [H]

Synonym: Bmul_0631

Alternate gene names: 161523811

Gene position: 692523-691375 (Counterclockwise)

Preceding gene: 161523813

Following gene: 161523809

Centisome position: 20.08

GC content: 66.14

Gene sequence:

>1149_bases
ATGAAAATCACCCGCCTCGAAACCTTCATCGTTCCGCCGCGCTGGCTGTTCCTCAAGATCGAAACCGACGAGGGCATCGT
CGGCTGGGGCGAGCCGGTCGTCGAAGGCCGCGCGCACACGGTCGAAGCCGCCGTGCACGAACTCGCCGACTACCTGGTCG
GCAAGGACCCGCGCCTGATCGAGGATCACTGGCAGGTGATGTACCGCGCGGGCTTCTACCGCGGCGGCCCGATCATGATG
AGCGCGATCGCGGGCGTCGATCAGGCGTTGTGGGACATCAAGGGCAAGCATCACGGCGTGCCCGTGCACGCGCTGCTCGG
CGGCCAGGTGCGCGAGCGCATCAAGGTCTATTCGTGGATCGGCGGCGACCGGCCGAGCGACGTCGCGAACAACGCGCGCG
CCGTGGTCGAGCGCGGCTTCAAGGCCGTGAAGATGAACGGCTCGGAAGAGCTGCAGATCGTCGATACCTACGACAAGGTC
GAGCAGGTGATCGCCAACGTCGCGGCCGTGCGCGACGCGGTGGGCCCGCACGTCGGAATCGGCGTCGACTTCCACGGCCG
CGTGCACAAGCCGATGGCGAAGGTGCTCGCGAAGGAACTCGATCCGTACAAGCTGATGTTCATCGAGGAGCCCGTGCTGT
CGGAGAACGCCGAGGCGCTGCGCGACATCGTGAATCAGACCAACACGCCGATCGCGCTCGGCGAGCGGCTCTACTCGCGC
TGGGACTTCAAGCACATCCTCGCCGGCGGCTACGTCGACATCATCCAGCCCGATGCATCGCATGCGGGCGGCATCACCGA
ATGCCGGAAGATCGCGACGCTCGCCGAAAGCTACGACGTCGCGCTCGCGCTGCACTGCCCGCTCGGGCCGATCGCGCTCG
CCGCGTGCCTGCAGCTCGACGCGGTCAGCTACAACGCGTTCATCCAGGAGCAGAGTCTCGGCATCCATTACAACCAGGGC
AACGATCTGCTCGACTATCTGCGCAATCCGGAAGTGTTCCGCTACGACGACGGCTTCGTCGCGATTCCGCAAGGGCCGGG
GCTCGGCATCGACGTGAACGAGGAGAAGGTGCGCGAGATGGCGAAGACCGGCCATCGCTGGCGCAATCCCGTGTGGCGCC
ACGCGGACGGCAGCGTCGCCGAGTGGTGA

Upstream 100 bases:

>100_bases
ATTAATACTATTTTTTAACGCGCTACGCTAGGATAACGCGAAAGCATGCGGCCGGCGTGGCGCCCATCGGGCACGCCGGT
CAACCCTGGAGACTCGCGAC

Downstream 100 bases:

>100_bases
GATGAGAGGTGCGGCGCATCGCGTGCGGGACGCGCCCGCGCGATGCGTCGATCGCGATACGGCCGCCGGTCGAACGACCG
GCGGCCTTTTTCATTGCGCG

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKITRLETFIVPPRWLFLKIETDEGIVGWGEPVVEGRAHTVEAAVHELADYLVGKDPRLIEDHWQVMYRAGFYRGGPIMM
SAIAGVDQALWDIKGKHHGVPVHALLGGQVRERIKVYSWIGGDRPSDVANNARAVVERGFKAVKMNGSEELQIVDTYDKV
EQVIANVAAVRDAVGPHVGIGVDFHGRVHKPMAKVLAKELDPYKLMFIEEPVLSENAEALRDIVNQTNTPIALGERLYSR
WDFKHILAGGYVDIIQPDASHAGGITECRKIATLAESYDVALALHCPLGPIALAACLQLDAVSYNAFIQEQSLGIHYNQG
NDLLDYLRNPEVFRYDDGFVAIPQGPGLGIDVNEEKVREMAKTGHRWRNPVWRHADGSVAEW

Sequences:

>Translated_382_residues
MKITRLETFIVPPRWLFLKIETDEGIVGWGEPVVEGRAHTVEAAVHELADYLVGKDPRLIEDHWQVMYRAGFYRGGPIMM
SAIAGVDQALWDIKGKHHGVPVHALLGGQVRERIKVYSWIGGDRPSDVANNARAVVERGFKAVKMNGSEELQIVDTYDKV
EQVIANVAAVRDAVGPHVGIGVDFHGRVHKPMAKVLAKELDPYKLMFIEEPVLSENAEALRDIVNQTNTPIALGERLYSR
WDFKHILAGGYVDIIQPDASHAGGITECRKIATLAESYDVALALHCPLGPIALAACLQLDAVSYNAFIQEQSLGIHYNQG
NDLLDYLRNPEVFRYDDGFVAIPQGPGLGIDVNEEKVREMAKTGHRWRNPVWRHADGSVAEW
>Mature_382_residues
MKITRLETFIVPPRWLFLKIETDEGIVGWGEPVVEGRAHTVEAAVHELADYLVGKDPRLIEDHWQVMYRAGFYRGGPIMM
SAIAGVDQALWDIKGKHHGVPVHALLGGQVRERIKVYSWIGGDRPSDVANNARAVVERGFKAVKMNGSEELQIVDTYDKV
EQVIANVAAVRDAVGPHVGIGVDFHGRVHKPMAKVLAKELDPYKLMFIEEPVLSENAEALRDIVNQTNTPIALGERLYSR
WDFKHILAGGYVDIIQPDASHAGGITECRKIATLAESYDVALALHCPLGPIALAACLQLDAVSYNAFIQEQSLGIHYNQG
NDLLDYLRNPEVFRYDDGFVAIPQGPGLGIDVNEEKVREMAKTGHRWRNPVWRHADGSVAEW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=382, Percent_Identity=61.7801047120419, Blast_Score=505, Evalue=1e-144,
Organism=Escherichia coli, GI1787864, Length=413, Percent_Identity=31.4769975786925, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI226510960, Length=268, Percent_Identity=30.5970149253731, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 42475; Mature: 42475

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITRLETFIVPPRWLFLKIETDEGIVGWGEPVVEGRAHTVEAAVHELADYLVGKDPRLI
CCCEEEEEEEECCEEEEEEEECCCCCEECCCHHHCCCHHHHHHHHHHHHHHHHCCCCCHH
EDHWQVMYRAGFYRGGPIMMSAIAGVDQALWDIKGKHHGVPVHALLGGQVRERIKVYSWI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHHHC
GGDRPSDVANNARAVVERGFKAVKMNGSEELQIVDTYDKVEQVIANVAAVRDAVGPHVGI
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEE
GVDFHGRVHKPMAKVLAKELDPYKLMFIEEPVLSENAEALRDIVNQTNTPIALGERLYSR
EECCCCCHHHHHHHHHHHHCCCEEEEEEECCHHCCCHHHHHHHHHCCCCCHHHHHHHHHH
WDFKHILAGGYVDIIQPDASHAGGITECRKIATLAESYDVALALHCPLGPIALAACLQLD
CCHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
AVSYNAFIQEQSLGIHYNQGNDLLDYLRNPEVFRYDDGFVAIPQGPGLGIDVNEEKVREM
HHHHHHEEEECCCCEEECCCCHHHHHHCCCCEEEECCCEEECCCCCCCCCCCCHHHHHHH
AKTGHRWRNPVWRHADGSVAEW
HHCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKITRLETFIVPPRWLFLKIETDEGIVGWGEPVVEGRAHTVEAAVHELADYLVGKDPRLI
CCCEEEEEEEECCEEEEEEEECCCCCEECCCHHHCCCHHHHHHHHHHHHHHHHCCCCCHH
EDHWQVMYRAGFYRGGPIMMSAIAGVDQALWDIKGKHHGVPVHALLGGQVRERIKVYSWI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCHHHHHHHHHHHHC
GGDRPSDVANNARAVVERGFKAVKMNGSEELQIVDTYDKVEQVIANVAAVRDAVGPHVGI
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCEEE
GVDFHGRVHKPMAKVLAKELDPYKLMFIEEPVLSENAEALRDIVNQTNTPIALGERLYSR
EECCCCCHHHHHHHHHHHHCCCEEEEEEECCHHCCCHHHHHHHHHCCCCCHHHHHHHHHH
WDFKHILAGGYVDIIQPDASHAGGITECRKIATLAESYDVALALHCPLGPIALAACLQLD
CCHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
AVSYNAFIQEQSLGIHYNQGNDLLDYLRNPEVFRYDDGFVAIPQGPGLGIDVNEEKVREM
HHHHHHEEEECCCCEEECCCCHHHHHHCCCCEEEECCCEEECCCCCCCCCCCCHHHHHHH
AKTGHRWRNPVWRHADGSVAEW
HHCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA