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The map label for this gene is glpK [H]

Identifier: 161523799

GI number: 161523799

Start: 677729

End: 679231

Strand: Reverse

Name: glpK [H]

Synonym: Bmul_0619

Alternate gene names: 161523799

Gene position: 679231-677729 (Counterclockwise)

Preceding gene: 161523800

Following gene: 161523798

Centisome position: 19.7

GC content: 67.47

Gene sequence:

>1503_bases
ATGCAGGACCAGTACATCCTCGCGCTCGACCAGGGCACGACGAGCTCCCGCGCGATGCTGTTCGATCGCCAGGGCAACAT
CGTGTCGATCGCCCAGAAAGAGTTCGAACAGATCTACCCGCAGCCCGGCTGGGTCGAGCACGACCCGCAGGAAATCTGGT
CGACGCAGGCGGGCGTCGCCGCCGAAGCCGTCACGCGCACCGGCCTGAACGGCACGTCGATCGCCGCGATCGGCATCACG
AACCAGCGCGAGACGACGATCGTCTGGGATCGCGAAACGGGCCAGCCCGTCTACAACGCGATCGTCTGGCAGGACCGCCG
CACCGCCGACTTCTGCGATTCGCTGAAAAAGCAGGGGCTCGAAGCGAAGGTGCGCGCGAAAACGGGCCTGCCGATCGACT
CGTACTTCTCCGCGACGAAGATCCGCTGGATCCTCGACAACGTGCCGGGCGCGCGCGACAAGGCGCGTCAGGGCAAGCTC
GCGTTCGGCACGGTCGACAGCTGGCTCGTGTGGAACTTCACGAAGCACGAGCTGCACGTGACCGACGTGACGAACGCGTC
GCGCACGATGCTGTTCAACATCCATACGCGCCAGTGGGACGACGAGCTGCTCGAACTGCTCGACATCCCGCGCAGCATGC
TGCCGGAAGTGAAGGCGTCGTCGGAGATCTACGGCCATACGAAGACCACGGTGTTCGCGTCGAAGATTCCGCTCGCGGGC
ATCGCGGGCGACCAGCACGCGGCGCTGTTCGGCCAGATGTGCACGACGTCCGGGATGGTGAAGAACACGTACGGCACCGG
CTGCTTCCTGATGATGAACACCGGCGACAAGCCGATCGAGTCGAAGAACAACCTCGTGACGACGATCGCCTGGCAGATCG
GCGACGACGTGCAGTACGCGCTCGAAGGCAGCATCTTCATCGCGGGCGCGGTCGTGCAGTGGCTGCGCGACGGCCTCGGC
ATCATCAAGACGGCCGCCGAGATCGAAGCGCTCGCCGCGAGCGTGCCGCACAGCGACGGCGTCTATCTGGTGCCCGCGTT
CGCCGGCCTCGGCGCGCCGCACTGGAACGCGCGCGCACGCGGCTCGCTGTTCGGCGTCACGCGCGGCACGCGCGACGCGC
ATCTCGCGCGCGCGGCGCTCGACGCGATCGCGTATCAGTCGCTCGACGTGCTGGCCGCGATGGAAGCCGACTCGGGCCTC
AGCATCGGCGAGCTGCGCGTCGACGGCGGCGCGAGCGCGAACAACCTGCTGATGCAGTTTCAGGCCGACCTGCTCGGCGT
CGATGCGGTGCGTCCGCAGATCACCGAGACGACGGCGCTCGGCGCCGCGTATCTCGCGGGCCTCGCGATCGGCTACTGGA
AGAACCTCGACGAAGTGCGCAGCCAGTGGCAGCTCGATCGCCGCTTCGCGCCGTCGATGCCCAAGCAGCAGGTCGAGCGC
TGCATGGCCGGCTGGCAGCGCGCGGTGCGTGCGGCAAAGGCCTGGGCCGACGACGCGCAGTAA

Upstream 100 bases:

>100_bases
GAGCGATCGCCCGTGCAGTTTGCTTGACCGACGTTGATTCACCGACGCGCCGCGGCATACGGCGCGCATCACAACTAATC
CACGGATGGAGATGAGAGAC

Downstream 100 bases:

>100_bases
GCGCCGCGTCGCGAACGACACAACAACAACGCCGGCGGACCGCGCACTGCGCGAGTCCGCCGCGATCTACGAGAGACAAA
CATGTCACCCTATATCGCAG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWSTQAGVAAEAVTRTGLNGTSIAAIGIT
NQRETTIVWDRETGQPVYNAIVWQDRRTADFCDSLKKQGLEAKVRAKTGLPIDSYFSATKIRWILDNVPGARDKARQGKL
AFGTVDSWLVWNFTKHELHVTDVTNASRTMLFNIHTRQWDDELLELLDIPRSMLPEVKASSEIYGHTKTTVFASKIPLAG
IAGDQHAALFGQMCTTSGMVKNTYGTGCFLMMNTGDKPIESKNNLVTTIAWQIGDDVQYALEGSIFIAGAVVQWLRDGLG
IIKTAAEIEALAASVPHSDGVYLVPAFAGLGAPHWNARARGSLFGVTRGTRDAHLARAALDAIAYQSLDVLAAMEADSGL
SIGELRVDGGASANNLLMQFQADLLGVDAVRPQITETTALGAAYLAGLAIGYWKNLDEVRSQWQLDRRFAPSMPKQQVER
CMAGWQRAVRAAKAWADDAQ

Sequences:

>Translated_500_residues
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWSTQAGVAAEAVTRTGLNGTSIAAIGIT
NQRETTIVWDRETGQPVYNAIVWQDRRTADFCDSLKKQGLEAKVRAKTGLPIDSYFSATKIRWILDNVPGARDKARQGKL
AFGTVDSWLVWNFTKHELHVTDVTNASRTMLFNIHTRQWDDELLELLDIPRSMLPEVKASSEIYGHTKTTVFASKIPLAG
IAGDQHAALFGQMCTTSGMVKNTYGTGCFLMMNTGDKPIESKNNLVTTIAWQIGDDVQYALEGSIFIAGAVVQWLRDGLG
IIKTAAEIEALAASVPHSDGVYLVPAFAGLGAPHWNARARGSLFGVTRGTRDAHLARAALDAIAYQSLDVLAAMEADSGL
SIGELRVDGGASANNLLMQFQADLLGVDAVRPQITETTALGAAYLAGLAIGYWKNLDEVRSQWQLDRRFAPSMPKQQVER
CMAGWQRAVRAAKAWADDAQ
>Mature_500_residues
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWSTQAGVAAEAVTRTGLNGTSIAAIGIT
NQRETTIVWDRETGQPVYNAIVWQDRRTADFCDSLKKQGLEAKVRAKTGLPIDSYFSATKIRWILDNVPGARDKARQGKL
AFGTVDSWLVWNFTKHELHVTDVTNASRTMLFNIHTRQWDDELLELLDIPRSMLPEVKASSEIYGHTKTTVFASKIPLAG
IAGDQHAALFGQMCTTSGMVKNTYGTGCFLMMNTGDKPIESKNNLVTTIAWQIGDDVQYALEGSIFIAGAVVQWLRDGLG
IIKTAAEIEALAASVPHSDGVYLVPAFAGLGAPHWNARARGSLFGVTRGTRDAHLARAALDAIAYQSLDVLAAMEADSGL
SIGELRVDGGASANNLLMQFQADLLGVDAVRPQITETTALGAAYLAGLAIGYWKNLDEVRSQWQLDRRFAPSMPKQQVER
CMAGWQRAVRAAKAWADDAQ

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=46.2151394422311, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=46.2151394422311, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI42794763, Length=507, Percent_Identity=45.7593688362919, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=44.8207171314741, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI88196792, Length=509, Percent_Identity=32.6129666011788, Blast_Score=309, Evalue=4e-84,
Organism=Escherichia coli, GI1790361, Length=497, Percent_Identity=58.5513078470825, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=498, Percent_Identity=24.4979919678715, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1789987, Length=490, Percent_Identity=24.0816326530612, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1789168, Length=495, Percent_Identity=25.0505050505051, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787789, Length=499, Percent_Identity=25.0501002004008, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535599, Length=500, Percent_Identity=43.8, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71986140, Length=515, Percent_Identity=23.495145631068, Blast_Score=168, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI71986147, Length=294, Percent_Identity=27.5510204081633, Blast_Score=118, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6321755, Length=555, Percent_Identity=35.6756756756757, Blast_Score=314, Evalue=2e-86,
Organism=Drosophila melanogaster, GI17864214, Length=508, Percent_Identity=41.9291338582677, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655555, Length=480, Percent_Identity=42.5, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655560, Length=480, Percent_Identity=42.5, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655570, Length=480, Percent_Identity=42.5, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655574, Length=480, Percent_Identity=42.5, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655565, Length=480, Percent_Identity=42.5, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24654573, Length=434, Percent_Identity=43.3179723502304, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI19922058, Length=527, Percent_Identity=33.5863377609108, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24652871, Length=494, Percent_Identity=34.412955465587, Blast_Score=280, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24656367, Length=514, Percent_Identity=30.3501945525292, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI21357867, Length=526, Percent_Identity=29.467680608365, Blast_Score=236, Evalue=4e-62,
Organism=Drosophila melanogaster, GI24656372, Length=358, Percent_Identity=30.1675977653631, Blast_Score=172, Evalue=6e-43,
Organism=Drosophila melanogaster, GI45552909, Length=298, Percent_Identity=28.8590604026846, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 54731; Mature: 54731

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWSTQAGVA
CCCCEEEEEECCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHH
AEAVTRTGLNGTSIAAIGITNQRETTIVWDRETGQPVYNAIVWQDRRTADFCDSLKKQGL
HHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHEEECCCCCHHHHHHHHHCCC
EAKVRAKTGLPIDSYFSATKIRWILDNVPGARDKARQGKLAFGTVDSWLVWNFTKHELHV
CEEEEECCCCCHHHHHHHHEEEEEECCCCCCCCHHHCCCEEEECCCCEEEEEEECCEEEE
TDVTNASRTMLFNIHTRQWDDELLELLDIPRSMLPEVKASSEIYGHTKTTVFASKIPLAG
EECCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCHHCCCCEEEEEECCCCEEE
IAGDQHAALFGQMCTTSGMVKNTYGTGCFLMMNTGDKPIESKNNLVTTIAWQIGDDVQYA
CCCCCHHHHHHHHHHCCCCEECCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCEEEE
LEGSIFIAGAVVQWLRDGLGIIKTAAEIEALAASVPHSDGVYLVPAFAGLGAPHWNARAR
ECCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCCCCCCCCC
GSLFGVTRGTRDAHLARAALDAIAYQSLDVLAAMEADSGLSIGELRVDGGASANNLLMQF
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCCCHHHHHHHH
QADLLGVDAVRPQITETTALGAAYLAGLAIGYWKNLDEVRSQWQLDRRFAPSMPKQQVER
HHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHH
CMAGWQRAVRAAKAWADDAQ
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWSTQAGVA
CCCCEEEEEECCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHH
AEAVTRTGLNGTSIAAIGITNQRETTIVWDRETGQPVYNAIVWQDRRTADFCDSLKKQGL
HHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHEEECCCCCHHHHHHHHHCCC
EAKVRAKTGLPIDSYFSATKIRWILDNVPGARDKARQGKLAFGTVDSWLVWNFTKHELHV
CEEEEECCCCCHHHHHHHHEEEEEECCCCCCCCHHHCCCEEEECCCCEEEEEEECCEEEE
TDVTNASRTMLFNIHTRQWDDELLELLDIPRSMLPEVKASSEIYGHTKTTVFASKIPLAG
EECCCCCEEEEEEEECCCCHHHHHHHHHCCHHHCCCCCCCCHHCCCCEEEEEECCCCEEE
IAGDQHAALFGQMCTTSGMVKNTYGTGCFLMMNTGDKPIESKNNLVTTIAWQIGDDVQYA
CCCCCHHHHHHHHHHCCCCEECCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCEEEE
LEGSIFIAGAVVQWLRDGLGIIKTAAEIEALAASVPHSDGVYLVPAFAGLGAPHWNARAR
ECCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCCCCCCCCC
GSLFGVTRGTRDAHLARAALDAIAYQSLDVLAAMEADSGLSIGELRVDGGASANNLLMQF
CCEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCCCHHHHHHHH
QADLLGVDAVRPQITETTALGAAYLAGLAIGYWKNLDEVRSQWQLDRRFAPSMPKQQVER
HHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHH
CMAGWQRAVRAAKAWADDAQ
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA