Click here to switch to the map view.

The map label for this gene is ptsN [H]

Identifier: 161523702

GI number: 161523702

Start: 571076

End: 571672

Strand: Reverse

Name: ptsN [H]

Synonym: Bmul_0522

Alternate gene names: 161523702

Gene position: 571672-571076 (Counterclockwise)

Preceding gene: 161523703

Following gene: 161523701

Centisome position: 16.58

GC content: 62.48

Gene sequence:

>597_bases
ATGCTCCGGTTATCGCCGGGGGGCGTTGATCCGGTCGCCGCGCGTTGCACGCTTCCGATGCCGAACGCCTTGATATCGTC
GAACATGGACCATCAGTCTGCCGCCGCAAGGAGACCCCAGGCCGTCCAATCGCCTGCCAACATGAATCGCCTAGCCAAAA
TCCTGCCCATAGAGAACGTCGTCATCGACCTCTCCGTCACCAGCAAGAAACGCGTTTTCGAACAAGCCGGGCTGATCTTC
GAGAATCAGAACGGCATCGCTCGCAGCACCGTCACGGACAATCTGTTCGCGCGCGAGCGTCTCGGCTCGACCGGTCTCGG
CGAAGGCGTCGCGATTCCGCACGGACGCATCAAGGGCCTCAAGCATCCGCTCGCGGCATTCGTGCGACTCGCCGATGCGA
TCCCGTTCGAGGCCCCCGACGGCCAGCCGGTTTCCCTCCTGATTTTCCTGCTCGTTCCCGAGCAAGCCACACAGCAGCAC
CTCGAGATCCTGTCCGAAATCGCGCAGCTGCTGTCCGATCGCGACGCGCGCGAGCGTCTGCACAACGAAACGGATCCCGC
CGAGTTGCATCGCCTGCTCACTCAGTGGCAACCTTGA

Upstream 100 bases:

>100_bases
ACATAGCGCCGCCCGGTTCGCCGGGCGTTTTTTTTTGCGGTGCCGCGCAGCGGCGCCGAGGGTCGGCCGCGCTGCACCAC
GCGCGCGGCTGTGTTCTATA

Downstream 100 bases:

>100_bases
GCTGATCCGGGCGGAATATTTTCCCGAACGACGCGGCCGGCGCCGCCGCGCACGGCCACCCGGCGCCGGTCCGGCATCGC
GACGCCCGTCGCCGTGCATC

Product: putative PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugarphosphate [C]

Alternate protein names: Enzyme IIA-NTR; Phosphotransferase enzyme IIA component; PTS system EIIA component [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MLRLSPGGVDPVAARCTLPMPNALISSNMDHQSAAARRPQAVQSPANMNRLAKILPIENVVIDLSVTSKKRVFEQAGLIF
ENQNGIARSTVTDNLFARERLGSTGLGEGVAIPHGRIKGLKHPLAAFVRLADAIPFEAPDGQPVSLLIFLLVPEQATQQH
LEILSEIAQLLSDRDARERLHNETDPAELHRLLTQWQP

Sequences:

>Translated_198_residues
MLRLSPGGVDPVAARCTLPMPNALISSNMDHQSAAARRPQAVQSPANMNRLAKILPIENVVIDLSVTSKKRVFEQAGLIF
ENQNGIARSTVTDNLFARERLGSTGLGEGVAIPHGRIKGLKHPLAAFVRLADAIPFEAPDGQPVSLLIFLLVPEQATQQH
LEILSEIAQLLSDRDARERLHNETDPAELHRLLTQWQP
>Mature_198_residues
MLRLSPGGVDPVAARCTLPMPNALISSNMDHQSAAARRPQAVQSPANMNRLAKILPIENVVIDLSVTSKKRVFEQAGLIF
ENQNGIARSTVTDNLFARERLGSTGLGEGVAIPHGRIKGLKHPLAAFVRLADAIPFEAPDGQPVSLLIFLLVPEQATQQH
LEILSEIAQLLSDRDARERLHNETDPAELHRLLTQWQP

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=149, Percent_Identity=34.8993288590604, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1786951, Length=99, Percent_Identity=34.3434343434343, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI2367327, Length=115, Percent_Identity=32.1739130434783, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1788726, Length=145, Percent_Identity=27.5862068965517, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 21663; Mature: 21663

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLSPGGVDPVAARCTLPMPNALISSNMDHQSAAARRPQAVQSPANMNRLAKILPIENV
CCCCCCCCCCHHHHEECCCCCHHHHHCCCCHHHHHHCCCHHHCCCCCHHHHHHHCCCCCE
VIDLSVTSKKRVFEQAGLIFENQNGIARSTVTDNLFARERLGSTGLGEGVAIPHGRIKGL
EEEEECCHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHH
KHPLAAFVRLADAIPFEAPDGQPVSLLIFLLVPEQATQQHLEILSEIAQLLSDRDARERL
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
HNETDPAELHRLLTQWQP
CCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLRLSPGGVDPVAARCTLPMPNALISSNMDHQSAAARRPQAVQSPANMNRLAKILPIENV
CCCCCCCCCCHHHHEECCCCCHHHHHCCCCHHHHHHCCCHHHCCCCCHHHHHHHCCCCCE
VIDLSVTSKKRVFEQAGLIFENQNGIARSTVTDNLFARERLGSTGLGEGVAIPHGRIKGL
EEEEECCHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHH
KHPLAAFVRLADAIPFEAPDGQPVSLLIFLLVPEQATQQHLEILSEIAQLLSDRDARERL
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
HNETDPAELHRLLTQWQP
CCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein Npi-phosphohistidine; sugar [C]

Specific reaction: Protein Npi-phosphohistidine + sugar = protein histidine + sugarphosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]