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The map label for this gene is dsbA [H]

Identifier: 161523563

GI number: 161523563

Start: 422318

End: 422956

Strand: Reverse

Name: dsbA [H]

Synonym: Bmul_0383

Alternate gene names: 161523563

Gene position: 422956-422318 (Counterclockwise)

Preceding gene: 161523564

Following gene: 161523562

Centisome position: 12.27

GC content: 62.91

Gene sequence:

>639_bases
ATGAAAAAACTGCTTAGCACGCTTCTTCTGTCCCTGGGCCTCGCGGCCGGCTTCGCGCAGGCGTCGCCGGCGGCGCCCGT
CGCCGGCAAGGACTTCGAGGTGATGAAGTCGCCGCAGCCGGTGTCCGCGCCGGCCGGCAAGGTCGAAGTGATCGAGTTCT
TCTGGTACGGCTGCCCGCACTGCTACGAGTTCGAGCCGACGATCGAAGCGTGGGTGAAGAAGCAAGGCGGCAACATCGAG
TTCAAGCGCGTGCCGGTCGCGTTCCGCGACGATTTCGTCCCGCACTCGCGGCTCTATTACGCGGTGTCCGCGCTCGGCAT
CGCCGAGAAGGTCACGCCGGCGATCTTCAACGCGATCCACAAGCAGAAGAACTATCTGCTGACGCCGCAGGCACAGGCCG
ACTTCCTCGCGACGCAAGGCGTCGACAAGAAGCAGTTCATGGATGCGTACAACTCGTTCAGCGTCCAAGGCCAGGTCAAG
CAGTCGGCCGAGCTGCTGAAGAACTACGCGATCGACGGCGTGCCGACGATCGTCGTCCAGGGCAAGTACAAGACGGGCCC
CGCCTACACGAACAGCATTCCGGGTACCGCGCAGGTGCTCGACTTCCTCGTGAAGCAGGTTCAGGACAAGAAGCTCTGA

Upstream 100 bases:

>100_bases
CACGGCAGTGATCCGTTTCACGAAGCAGTAAGCATCCGGCCGCGCCCACGCAGAACCCGTTACGTTTCGTAACCCGAACA
ACCGATTCGACGTACACAAC

Downstream 100 bases:

>100_bases
TCCTCGCCCGCGCGCCGGCATGACTACTCCGCTGAAGGTCTTCATCACCGGCGCGTCGAGCGGCCTCGGCCTCGCGATGG
CCGAAGAATACGCCCGCCAG

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKKLLSTLLLSLGLAAGFAQASPAAPVAGKDFEVMKSPQPVSAPAGKVEVIEFFWYGCPHCYEFEPTIEAWVKKQGGNIE
FKRVPVAFRDDFVPHSRLYYAVSALGIAEKVTPAIFNAIHKQKNYLLTPQAQADFLATQGVDKKQFMDAYNSFSVQGQVK
QSAELLKNYAIDGVPTIVVQGKYKTGPAYTNSIPGTAQVLDFLVKQVQDKKL

Sequences:

>Translated_212_residues
MKKLLSTLLLSLGLAAGFAQASPAAPVAGKDFEVMKSPQPVSAPAGKVEVIEFFWYGCPHCYEFEPTIEAWVKKQGGNIE
FKRVPVAFRDDFVPHSRLYYAVSALGIAEKVTPAIFNAIHKQKNYLLTPQAQADFLATQGVDKKQFMDAYNSFSVQGQVK
QSAELLKNYAIDGVPTIVVQGKYKTGPAYTNSIPGTAQVLDFLVKQVQDKKL
>Mature_212_residues
MKKLLSTLLLSLGLAAGFAQASPAAPVAGKDFEVMKSPQPVSAPAGKVEVIEFFWYGCPHCYEFEPTIEAWVKKQGGNIE
FKRVPVAFRDDFVPHSRLYYAVSALGIAEKVTPAIFNAIHKQKNYLLTPQAQADFLATQGVDKKQFMDAYNSFSVQGQVK
QSAELLKNYAIDGVPTIVVQGKYKTGPAYTNSIPGTAQVLDFLVKQVQDKKL

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins. Involved in the production of protease and alkaline phosphatase, motility, metal resistance, and multi-drug resistance [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioredoxin family. DsbA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=214, Percent_Identity=28.9719626168224, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 23277; Mature: 23277

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLSTLLLSLGLAAGFAQASPAAPVAGKDFEVMKSPQPVSAPAGKVEVIEFFWYGCPH
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCC
CYEFEPTIEAWVKKQGGNIEFKRVPVAFRDDFVPHSRLYYAVSALGIAEKVTPAIFNAIH
CCCCCHHHHHHHHHCCCCEEEEECCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KQKNYLLTPQAQADFLATQGVDKKQFMDAYNSFSVQGQVKQSAELLKNYAIDGVPTIVVQ
HHCCEEECCCCHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEE
GKYKTGPAYTNSIPGTAQVLDFLVKQVQDKKL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLLSTLLLSLGLAAGFAQASPAAPVAGKDFEVMKSPQPVSAPAGKVEVIEFFWYGCPH
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCC
CYEFEPTIEAWVKKQGGNIEFKRVPVAFRDDFVPHSRLYYAVSALGIAEKVTPAIFNAIH
CCCCCHHHHHHHHHCCCCEEEEECCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KQKNYLLTPQAQADFLATQGVDKKQFMDAYNSFSVQGQVKQSAELLKNYAIDGVPTIVVQ
HHCCEEECCCCHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEE
GKYKTGPAYTNSIPGTAQVLDFLVKQVQDKKL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10711598 [H]