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The map label for this gene is bioF [H]

Identifier: 161523553

GI number: 161523553

Start: 409672

End: 410856

Strand: Reverse

Name: bioF [H]

Synonym: Bmul_0373

Alternate gene names: 161523553

Gene position: 410856-409672 (Counterclockwise)

Preceding gene: 161523554

Following gene: 161523552

Centisome position: 11.91

GC content: 72.49

Gene sequence:

>1185_bases
ATGAACCTGCTCGATACGCTGCAGCGCGGCCTCGCCGATCTCGACGCGCAAGGGCTGCGCCGTGTGCGCCGCACGGCCGA
CAGCGCCTGCGACGCGCACATGACCGTGAACGGCCGCGAGATCGTCGGGTTCGCGAGCAACGACTACCTCGGCCTCGCCG
CGCATCCGAAACTTGTCGCCGCGTTCGCCGAAGGCGCGCAGCGCTACGGCTCCGGCAGCGGCGGCTCGCATCTGCTCGGC
GGCCACTCGCGTGCGCACGCGAAGCTCGAGGACGAACTCGCGGGCTTCGCGGGCGGCTTCTCCGATGCGCCGCGCGCGCT
GTACTTCAGCACCGGCTACATGGCGAACCTCGCGGCGGTCACCGCGCTCGCCGGCAAGGACGCGACGATCTTCTCCGACG
CGCTGAACCACGCGTCGCTGATCGACGGCACGCGGCTGTCGCGCGCCACCGTGCAGGTCTATCCGCATGCCGATACGGCC
ACGCTCGGCGCGTTGCTCGAGGCATGCACGTCGCAGACGAAGCTGATCGTCACCGATACGGTGTTCAGCATGGACGGCGA
CATCGCGCCGCTCGCCGAACTCCTCGCGCTCGCGGAACGCCACGGCGCATGGCTCGTCGTCGACGACGCGCACGGCTTCG
GCGTGCTCGGCCCGCAAGGCCGCGGCGCGCTCGCGGCGGCCGCGCTGCGCTCGCCGCATCTCGTCTACGTCGGCACGCTC
GGCAAGGCGGCCGGCGTCGCGGGCGCATTCGTCGTCGCGCACGAGACGGTGATCGAGTGGCTGATCCAGCGCGCGCGCAG
CTACATCTTCACGACGGCCGCACCGCCGGCCGTCGCGCATGCGGTGTCGGCGAGCCTGAAGGTGATCGCCGGCGACGAAG
GCGATGCGCGGCGCGCGCACCTCGCCGCGCTGATCGAACGCACGCGCGCGCTGCTGCGCCGCACGCGCTGGCAGCCGGTC
GACTCGCACACGGCCGTGCAGCCGCTCGTGATCGGCAGCAACGAGGCGACGCTCGCCGCGATGCGCGCGCTCGACGCGCA
CGGCCTGTGGGTGCCCGCGATCCGGCCGCCGACGGTGCCGGCCGGCACCTCGCGGCTACGCATCTCGCTGTCGGCCGCGC
ATTCGTTCGACGATCTCGCGCGGCTCGAAACCGCGCTGCTGCGCGCAAGCGAGGAGGCCGCATGA

Upstream 100 bases:

>100_bases
TCTACCTGATGCCGCCGTACGTGATGAGCGACGACGACATCGCCTGGCTCGCGCAGCGCACGCGCGACACGCTCGAGGCC
ACGCTCGCGGAGATTGCACG

Downstream 100 bases:

>100_bases
GCACACCGCTTTCGCTGTTCGTCACGGGCACCGACACCGAGATCGGCAAGACCTTCGTGTCGGCCGCGCTGCTGCACGGC
TTCGCACGGCACGGGCTGCG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNLLDTLQRGLADLDAQGLRRVRRTADSACDAHMTVNGREIVGFASNDYLGLAAHPKLVAAFAEGAQRYGSGSGGSHLLG
GHSRAHAKLEDELAGFAGGFSDAPRALYFSTGYMANLAAVTALAGKDATIFSDALNHASLIDGTRLSRATVQVYPHADTA
TLGALLEACTSQTKLIVTDTVFSMDGDIAPLAELLALAERHGAWLVVDDAHGFGVLGPQGRGALAAAALRSPHLVYVGTL
GKAAGVAGAFVVAHETVIEWLIQRARSYIFTTAAPPAVAHAVSASLKVIAGDEGDARRAHLAALIERTRALLRRTRWQPV
DSHTAVQPLVIGSNEATLAAMRALDAHGLWVPAIRPPTVPAGTSRLRISLSAAHSFDDLARLETALLRASEEAA

Sequences:

>Translated_394_residues
MNLLDTLQRGLADLDAQGLRRVRRTADSACDAHMTVNGREIVGFASNDYLGLAAHPKLVAAFAEGAQRYGSGSGGSHLLG
GHSRAHAKLEDELAGFAGGFSDAPRALYFSTGYMANLAAVTALAGKDATIFSDALNHASLIDGTRLSRATVQVYPHADTA
TLGALLEACTSQTKLIVTDTVFSMDGDIAPLAELLALAERHGAWLVVDDAHGFGVLGPQGRGALAAAALRSPHLVYVGTL
GKAAGVAGAFVVAHETVIEWLIQRARSYIFTTAAPPAVAHAVSASLKVIAGDEGDARRAHLAALIERTRALLRRTRWQPV
DSHTAVQPLVIGSNEATLAAMRALDAHGLWVPAIRPPTVPAGTSRLRISLSAAHSFDDLARLETALLRASEEAA
>Mature_394_residues
MNLLDTLQRGLADLDAQGLRRVRRTADSACDAHMTVNGREIVGFASNDYLGLAAHPKLVAAFAEGAQRYGSGSGGSHLLG
GHSRAHAKLEDELAGFAGGFSDAPRALYFSTGYMANLAAVTALAGKDATIFSDALNHASLIDGTRLSRATVQVYPHADTA
TLGALLEACTSQTKLIVTDTVFSMDGDIAPLAELLALAERHGAWLVVDDAHGFGVLGPQGRGALAAAALRSPHLVYVGTL
GKAAGVAGAFVVAHETVIEWLIQRARSYIFTTAAPPAVAHAVSASLKVIAGDEGDARRAHLAALIERTRALLRRTRWQPV
DSHTAVQPLVIGSNEATLAAMRALDAHGLWVPAIRPPTVPAGTSRLRISLSAAHSFDDLARLETALLRASEEAA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=390, Percent_Identity=33.3333333333333, Blast_Score=198, Evalue=9e-51,
Organism=Homo sapiens, GI284448556, Length=363, Percent_Identity=33.8842975206612, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI83977444, Length=385, Percent_Identity=34.5454545454545, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI83977442, Length=385, Percent_Identity=34.8051948051948, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI83977440, Length=385, Percent_Identity=34.8051948051948, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI4502025, Length=344, Percent_Identity=31.9767441860465, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI40316939, Length=344, Percent_Identity=31.9767441860465, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4758668, Length=368, Percent_Identity=29.8913043478261, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI119220554, Length=368, Percent_Identity=29.0760869565217, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI5454084, Length=276, Percent_Identity=26.8115942028986, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1786993, Length=353, Percent_Identity=45.8923512747875, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1790046, Length=383, Percent_Identity=35.2480417754569, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI71994529, Length=389, Percent_Identity=32.9048843187661, Blast_Score=184, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI32566772, Length=407, Percent_Identity=26.7813267813268, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17560914, Length=376, Percent_Identity=26.3297872340426, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17560912, Length=376, Percent_Identity=26.3297872340426, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71982625, Length=287, Percent_Identity=25.0871080139373, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71982617, Length=287, Percent_Identity=25.0871080139373, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320438, Length=410, Percent_Identity=30.2439024390244, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6320267, Length=406, Percent_Identity=27.3399014778325, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6323954, Length=308, Percent_Identity=27.5974025974026, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24662918, Length=394, Percent_Identity=31.7258883248731, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI17137420, Length=357, Percent_Identity=31.3725490196078, Blast_Score=171, Evalue=8e-43,
Organism=Drosophila melanogaster, GI17136286, Length=374, Percent_Identity=26.7379679144385, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24653280, Length=276, Percent_Identity=25.7246376811594, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653276, Length=276, Percent_Identity=25.7246376811594, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653278, Length=276, Percent_Identity=25.7246376811594, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 41199; Mature: 41199

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLDTLQRGLADLDAQGLRRVRRTADSACDAHMTVNGREIVGFASNDYLGLAAHPKLVA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCEEEEECCHHHHH
AFAEGAQRYGSGSGGSHLLGGHSRAHAKLEDELAGFAGGFSDAPRALYFSTGYMANLAAV
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHH
TALAGKDATIFSDALNHASLIDGTRLSRATVQVYPHADTATLGALLEACTSQTKLIVTDT
HHHCCCCCHHHHHHHCHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEE
VFSMDGDIAPLAELLALAERHGAWLVVDDAHGFGVLGPQGRGALAAAALRSPHLVYVGTL
EECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCEEEEECC
GKAAGVAGAFVVAHETVIEWLIQRARSYIFTTAAPPAVAHAVSASLKVIAGDEGDARRAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCEEEEECCCCCHHHHH
LAALIERTRALLRRTRWQPVDSHTAVQPLVIGSNEATLAAMRALDAHGLWVPAIRPPTVP
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCEEECCCCCCCCC
AGTSRLRISLSAAHSFDDLARLETALLRASEEAA
CCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNLLDTLQRGLADLDAQGLRRVRRTADSACDAHMTVNGREIVGFASNDYLGLAAHPKLVA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCEEEEECCHHHHH
AFAEGAQRYGSGSGGSHLLGGHSRAHAKLEDELAGFAGGFSDAPRALYFSTGYMANLAAV
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHH
TALAGKDATIFSDALNHASLIDGTRLSRATVQVYPHADTATLGALLEACTSQTKLIVTDT
HHHCCCCCHHHHHHHCHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEE
VFSMDGDIAPLAELLALAERHGAWLVVDDAHGFGVLGPQGRGALAAAALRSPHLVYVGTL
EECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCEEEEECC
GKAAGVAGAFVVAHETVIEWLIQRARSYIFTTAAPPAVAHAVSASLKVIAGDEGDARRAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHCCEEEEECCCCCHHHHH
LAALIERTRALLRRTRWQPVDSHTAVQPLVIGSNEATLAAMRALDAHGLWVPAIRPPTVP
HHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCEEECCCCCCCCC
AGTSRLRISLSAAHSFDDLARLETALLRASEEAA
CCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA