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The map label for this gene is bioB [H]

Identifier: 161523551

GI number: 161523551

Start: 407896

End: 408915

Strand: Reverse

Name: bioB [H]

Synonym: Bmul_0371

Alternate gene names: 161523551

Gene position: 408915-407896 (Counterclockwise)

Preceding gene: 161523552

Following gene: 161523549

Centisome position: 11.86

GC content: 67.84

Gene sequence:

>1020_bases
ATGACCCAAGCCCAGACCGCCGCCGCGCAACCCGACGCGATTCCCGTGGCAGCGCCGTCGCCGCAGCGCTGGCGCGTCGC
CGACGTCGTCGCGCTGTTCGAGCTGCCGTTCAACGATCTGATCTTCCGTGCGCAGCAGGTGCATCGCGAGCACTTCGACG
CGAACGCCGTGCAGCTGTCGACGCTGCTGTCGATCAAGACCGGCGGCTGCGAGGAAGACTGCGGCTATTGCTCGCAGTCC
GCGCATCACGACACGGGCCTGAAGGCCGAGAAGCTGATGGACGTCGACGCGGTGCTCGACGCCGCGCGCGCCGCGAAGGC
GAACGGCGCGAGCCGCTTCTGCATGGGCGCCGCATGGCGCAACCCGAAGGAGCGCCACATGCCTGCGCTGACCGAGATGG
TGCGCGGCGTGAAGGCGCTCGGCCTCGAGACCTGCATGACGCTCGGGATGCTCGAGGACGAACAGGCGAAGCAGCTCGCC
GACGCGGGCCTCGACTACTACAACCACAACCTCGACACGTCGCCGGAGTTCTACGGGCAGGTGATCTCGACGCGCACGTA
CCAGGACCGCCTCGACACGCTCGACCGCGTGCGCGACGCCGGCATCAACGTGTGCTGCGGCGGCATCATCGGGATGGGCG
AATCGCGCCGCGAACGCGCGGGGTTGATCTCGCAACTCGCGAACCTGAATCCGTATCCGGAATCGGTGCCGATCAACAAT
CTGGTCGCGATCGAAGGCACGCCGCTCGCCGGCACCGCGCCGCTCGATCCGTTCGAATTCGTGCGCACGATCGCCGTTGC
GCGCATCACGATGCCGAAGGCGGTCGTGCGTCTGTCGGCCGGCCGCGAACAGCTCGACGACGGCCTGCAGGCGCTCTGCT
TCCTCGCCGGCGCGAACTCGATGTTCTACGGCGACCAGCTGCTGACGACGAGCAACCCGCAGACGCAGAAGGATCGCGCG
CTGTTCGAGCGTCTCGGAATTCGCGCGAGCGAGGCCGATGCGATGTCGGCCGACGCGTAA

Upstream 100 bases:

>100_bases
GCCGCGGCGACGCTCGACATCGCCGCGCTCGTCGACACGTTGCGCGCCGCGCAGCGTTGACTCCCCGATCTCACCCCTCC
AGTCAGGACAGGAAAACGAA

Downstream 100 bases:

>100_bases
GCGCGCAGCGCACCGCGGGTTCGCCCGCCCGATGAAAAAGCCGGGACGTGTCCCGGCTTTTTCGTTGCCGTACGGTTCGC
ATACCTGCACGGCACGGCGG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTQAQTAAAQPDAIPVAAPSPQRWRVADVVALFELPFNDLIFRAQQVHREHFDANAVQLSTLLSIKTGGCEEDCGYCSQS
AHHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAWRNPKERHMPALTEMVRGVKALGLETCMTLGMLEDEQAKQLA
DAGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPESVPINN
LVAIEGTPLAGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQALCFLAGANSMFYGDQLLTTSNPQTQKDRA
LFERLGIRASEADAMSADA

Sequences:

>Translated_339_residues
MTQAQTAAAQPDAIPVAAPSPQRWRVADVVALFELPFNDLIFRAQQVHREHFDANAVQLSTLLSIKTGGCEEDCGYCSQS
AHHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAWRNPKERHMPALTEMVRGVKALGLETCMTLGMLEDEQAKQLA
DAGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPESVPINN
LVAIEGTPLAGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQALCFLAGANSMFYGDQLLTTSNPQTQKDRA
LFERLGIRASEADAMSADA
>Mature_338_residues
TQAQTAAAQPDAIPVAAPSPQRWRVADVVALFELPFNDLIFRAQQVHREHFDANAVQLSTLLSIKTGGCEEDCGYCSQSA
HHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAWRNPKERHMPALTEMVRGVKALGLETCMTLGMLEDEQAKQLAD
AGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPESVPINNL
VAIEGTPLAGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQALCFLAGANSMFYGDQLLTTSNPQTQKDRAL
FERLGIRASEADAMSADA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=316, Percent_Identity=63.6075949367089, Blast_Score=439, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6321725, Length=339, Percent_Identity=48.377581120944, Blast_Score=334, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36774; Mature: 36643

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAQTAAAQPDAIPVAAPSPQRWRVADVVALFELPFNDLIFRAQQVHREHFDANAVQLS
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
TLLSIKTGGCEEDCGYCSQSAHHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAWR
HHHHHCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
NPKERHMPALTEMVRGVKALGLETCMTLGMLEDEQAKQLADAGLDYYNHNLDTSPEFYGQ
CCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHCCCCCCCHHHHHH
VISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPESVPINN
HHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
LVAIEGTPLAGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQALCFLAGANS
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
MFYGDQLLTTSNPQTQKDRALFERLGIRASEADAMSADA
CEECCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQAQTAAAQPDAIPVAAPSPQRWRVADVVALFELPFNDLIFRAQQVHREHFDANAVQLS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHH
TLLSIKTGGCEEDCGYCSQSAHHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAWR
HHHHHCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
NPKERHMPALTEMVRGVKALGLETCMTLGMLEDEQAKQLADAGLDYYNHNLDTSPEFYGQ
CCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHHHHCCCCCCCHHHHHH
VISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPESVPINN
HHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
LVAIEGTPLAGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQALCFLAGANS
EEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
MFYGDQLLTTSNPQTQKDRALFERLGIRASEADAMSADA
CEECCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA