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The map label for this gene is norB [H]

Identifier: 161509603

GI number: 161509603

Start: 1502421

End: 1503812

Strand: Reverse

Name: norB [H]

Synonym: USA300HOU_1373

Alternate gene names: 161509603

Gene position: 1503812-1502421 (Counterclockwise)

Preceding gene: 161509604

Following gene: 161509602

Centisome position: 52.34

GC content: 32.83

Gene sequence:

>1392_bases
ATGGAAAAGCCGTCAAGAGAGGCATTTGAAGGCAATAATAAGTTGTTAATAGGAATTGTTCTAAGTGTAATAACGTTTTG
GCTATTTGCACAATCATTGGTTAATGTTGTACCAATACTTGAAGATAGTTTCAATACAGATATTGGAACGGTTAATATCG
CCGTTAGTATAACTGCTTTATTTTCAGGAATGTTTGTAGTAGGAGCAGGTGGTCTTGCTGATAAATATGGCAGAATTAAA
CTCACGAACATTGGTATTATCTTAAATATATTAGGTTCATTATTAATCATTATTTCAAATATTCCTTTATTACTTATTAT
AGGAAGATTAATTCAAGGACTTTCAGCAGCATGTATTATGCCTGCAACTTTGTCTATTATTAAGTCATATTACATTGGGA
AAGATAGACAACGCGCTTTAAGTTATTGGTCAATTGGCTCATGGGGCGGCTCTGGTGTTTGTTCATTTTTTGGAGGTGCA
GTTGCAACGCTTTTAGGTTGGCGTTGGATTTTCATCCTATCAATTATAATTTCATTAATTGCACTGTTTCTTATTAAAGG
CACACCTGAAACTAAATCTAAATCGATTTCTCTAAATAAATTTGACATTAAAGGTCTGGTTCTTTTAGTCATTATGCTCC
TCAGTTTAAATATTTTAATTACTAAAGGATCAGAATTAGGTGTAACCTCACTTCTTTTTATTACTTTATTAGCTATTGCA
ATTGGATCTTTTAGTTTATTTATAGTTCTTGAAAAGCGTGCTACAAATCCTTTAATCGATTTTAAATTATTTAAAAATAA
AGCTTACACAGGTGCAACAGCTTCAAACTTTTTGTTAAATGGTGTTGCAGGAACATTAATAGTAGCCAACACATTTGTTC
AAAGAGGTTTAGGATATTCTTCATTGCAAGCAGGAAGTTTATCAATCACTTATTTAGTAATGGTACTAATTATGATTCGT
GTTGGTGAAAAGTTACTTCAAACACTCGGATGCAAGAAACCAATGTTAATTGGAACAGGAGTTCTTATTGTCGGAGAATG
TCTCATTTCATTAACTTTCTTGCCAGAAATATTCTATGTCATTTGTTGTATTATAGGTTATTTATTCTTTGGTTTAGGAC
TAGGGATATATGCTACACCATCAACAGATACAGCAATTGCAAATGCACCGTTAGAAAAAGTAGGCGTTGCTGCAGGTATC
TATAAAATGGCTTCTGCATTAGGTGGAGCATTTGGCGTCGCATTGAGTGGTGCAGTATATGCAATCGTATCAAATATGAC
AAACATTTATACAGGTGCAATGATTGCATTATGGTTAAATGCAGGTATGGGAATATTATCATTCGTTATCATTTTGTTAC
TTGTGCCTAAACAAAACGATACTCAATTATGA

Upstream 100 bases:

>100_bases
TACACATTAAAATCAAAAACCATTCAATTATTCTATGGAACAGACAAATTTCTGTTATGGAATTTGTCTGTTTTTCAAAA
GTATAGGGAGGCAAATAGAG

Downstream 100 bases:

>100_bases
TAATTGAGAATTAAATTGAAATCATACAAGTCGCTACAATATTAAACAAAAATATAAACCGATTCTTATGTGTCATTATT
TTAAATGAACATAGGGATTG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSFNTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIK
LTNIGIILNILGSLLIIISNIPLLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNILITKGSELGVTSLLFITLLAIA
IGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASNFLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIR
VGEKLLQTLGCKKPMLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLEKVGVAAGI
YKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVIILLLVPKQNDTQL

Sequences:

>Translated_463_residues
MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSFNTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIK
LTNIGIILNILGSLLIIISNIPLLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNILITKGSELGVTSLLFITLLAIA
IGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASNFLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIR
VGEKLLQTLGCKKPMLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLEKVGVAAGI
YKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVIILLLVPKQNDTQL
>Mature_463_residues
MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSFNTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIK
LTNIGIILNILGSLLIIISNIPLLLIIGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNILITKGSELGVTSLLFITLLAIA
IGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASNFLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIR
VGEKLLQTLGCKKPMLIGTGVLIVGECLISLTFLPEIFYVICCIIGYLFFGLGLGIYATPSTDTAIANAPLEKVGVAAGI
YKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVIILLLVPKQNDTQL

Specific function: Multidrug efflux pump that acts independently of norA and is one of the factors that confers resistance against diverse quinolones and chemical compounds [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=410, Percent_Identity=24.1463414634146, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788710, Length=329, Percent_Identity=25.8358662613982, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789042, Length=430, Percent_Identity=21.8604651162791, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081983, Length=354, Percent_Identity=23.728813559322, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324954, Length=235, Percent_Identity=25.9574468085106, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49294; Mature: 49294

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSFNTDIGTVNIAVSITAL
CCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHEEHHHH
FSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIPLLLIIGRLIQGLSAACIM
HCCHHHEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHH
PATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGAVATLLGWRWIFILSIIISLI
HHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNILITKGSELGVTSLLFITLLAIA
HHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHH
IGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASNFLLNGVAGTLIVANTFVQRGLGYS
HCCEEEEEEEECCCCCCCEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
SLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKPMLIGTGVLIVGECLISLTFLPEIFYV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHH
ICCIIGYLFFGLGLGIYATPSTDTAIANAPLEKVGVAAGIYKMASALGGAFGVALSGAVY
HHHHHHHHHHHHCCCEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIVSNMTNIYTGAMIALWLNAGMGILSFVIILLLVPKQNDTQL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEKPSREAFEGNNKLLIGIVLSVITFWLFAQSLVNVVPILEDSFNTDIGTVNIAVSITAL
CCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHEEHHHH
FSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIPLLLIIGRLIQGLSAACIM
HCCHHHEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHH
PATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGAVATLLGWRWIFILSIIISLI
HHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNILITKGSELGVTSLLFITLLAIA
HHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHH
IGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASNFLLNGVAGTLIVANTFVQRGLGYS
HCCEEEEEEEECCCCCCCEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
SLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKPMLIGTGVLIVGECLISLTFLPEIFYV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHH
ICCIIGYLFFGLGLGIYATPSTDTAIANAPLEKVGVAAGIYKMASALGGAFGVALSGAVY
HHHHHHHHHHHHCCCEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIVSNMTNIYTGAMIALWLNAGMGILSFVIILLLVPKQNDTQL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA