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The map label for this gene is brnQ2 [C]

Identifier: 161509572

GI number: 161509572

Start: 1443780

End: 1445123

Strand: Reverse

Name: brnQ2 [C]

Synonym: USA300HOU_1342

Alternate gene names: 161509572

Gene position: 1445123-1443780 (Counterclockwise)

Preceding gene: 161509573

Following gene: 161509571

Centisome position: 50.3

GC content: 32.22

Gene sequence:

>1344_bases
ATGAATAAAAATACATGGGTCATTGGGTTTACGCTCTTCGCTATGTTTTTTGGCGCAGGCAATCTAATTTTTCCTCCTAA
TCTCGGATTGGATAGTGGTCAATTTTTCTGGCCCGCCATTTTAGCGTTTGTTCTAACTGGGATTGGTTTACCATTATTAG
GTGTGATTGTAGGTGCACTTGATAAAGAAGGATATATTGGCGCATTAAATAAAATTTCACCTAAATTTTCAATATTGTTC
TTAATCATCATTTATTTGACTATAGGACCACTTTTTGCAATACCTAGAACTGCATCTACATCTTTTGAAATGACAATTAC
ACCAATTATACATAGCAATAGTAGTATCGCTTTATTTATATTTACGATTATCTACTTCATAGTCGTTTTGTATATTTGTT
TAAATCCATCTAAGTTAATCGATCGTATTGGTTCATTATTAACACCATTATTATTGATTACTATTTTAGCGATGATTATT
AAAGGATACTTAGACTTTAGCGGTAATAGTGCTGGAAAGGGCAATGAAGCACTATATCATTCTAATTTTTCAAGTTTTGC
TGAAGGCTTTACACAAGGCTATTTAACAATGGATGCCATTGCAGCAATTGCTTTTTCAATGATTGTTGTTAATGCAGTAA
AACTAACAGGCATTACTAAAACAAATCAAATATTCAAACAAACTTTGACTGCTGGTTTAATTGCAGCCGTAGCTTTAATT
TTCATATATATTTCATTAGGTTATATTGGTAATCATATGCCAGTAAGTGACATGACGTTAGATCAATTGAAATCCAAAGA
TCGAAACATTGGGACATATTTATTAACGACAATGGCTTCAACAGGATTTGGTTCATTCGGAAAATATTTATTGGGCATCA
TTGTGGCGCTGGCATGTCTAACTACAGCATGCGGGCTTATTGTTGCAGTTTCTGAATATTTCCATAGAATCGTACCTAAA
GTATCATACAAAGCATTTGTATTAGTTTTCATTTTAATGAGTTTTATTATTGCTAACCAAGGTTTAAATGCTGTTATCTC
AATGTCAATTCCGGTATTAAGCATTGTATACCCAGTAGCAATAACTGTTGTATTATTAATTTTAATTGCCAAATTCATAC
CGACAAAACGCATTTCACAACAAATTCCAGTTATTATCGTATTTATATTGTCGATTTTCAGTGTTATTAGTAAGTTAGGT
TGGCTGAAAATTAACTTTATAGAATCATTGCCTCTAAGAGCGTATTCTTTAGAGTGGTTCCCAGTAGCAATTATTGCAAC
GATATTAGGCTATCTAGTCGGCATATTTGTAAAACAAGATCCAATTAAATATCAACAGGAATAA

Upstream 100 bases:

>100_bases
TCTGCTATGTACAACATATTTCATATAGACTCTTTATTATTTTTTGTGCTAAAATATTTACTTGTAAATTATTTCGTGTT
TTAAAAAAAGGAGTAACTCT

Downstream 100 bases:

>100_bases
CGAATAATATAAAAGAGGTTGGGACATAAATCCCTAAAAAAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTTACT
GCTGTTCTCTATTTATACAA

Product: LIVCS family branched chain amino acid:cation symporter

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILF
LIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPK
VSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLG
WLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE

Sequences:

>Translated_447_residues
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILF
LIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPK
VSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLG
WLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE
>Mature_447_residues
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILF
LIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASTGFGSFGKYLLGIIVALACLTTACGLIVAVSEYFHRIVPK
VSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLG
WLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine), which is coupled to a proton motive force (Potential). Contributes to NaCl tolerance

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family

Homologues:

Organism=Escherichia coli, GI1786601, Length=434, Percent_Identity=33.4101382488479, Blast_Score=232, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BRNQL_STAA3 (Q2FH31)

Other databases:

- EMBL:   CP000255
- RefSeq:   YP_493997.1
- ProteinModelPortal:   Q2FH31
- STRING:   Q2FH31
- EnsemblBacteria:   EBSTAT00000036784
- GeneID:   3914869
- GenomeReviews:   CP000255_GR
- KEGG:   saa:SAUSA300_1300
- eggNOG:   COG1114
- GeneTree:   EBGT00050000023663
- HOGENOM:   HBG533860
- OMA:   YLSTIWV
- ProtClustDB:   CLSK870647
- InterPro:   IPR004685
- TIGRFAMs:   TIGR00796

Pfam domain/function: PF05525 Branch_AA_trans

EC number: NA

Molecular weight: Translated: 48815; Mature: 48815

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x15927720)-; HASH(0x166849b0)-; HASH(0x166a6488)-; HASH(0x16058100)-; HASH(0x15dd41cc)-; HASH(0x160b7f2c)-; HASH(0x156a82f0)-; HASH(0x15e25f80)-; HASH(0x16698b20)-; HASH(0x1554288c)-; HASH(0x14d88740)-; HASH(0x164d6680)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIGLPLLGVIVGAL
CCCCCEEHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
DKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFI
CCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCHHHHH
FTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMIIKGYLDFSGNSAGKGNEALYH
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
SNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASTGFGSFGKYLLGIIVALACL
HHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLGWLKINFIESLPLRAYSLEWF
HHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEHHHCCCCCEEECCHH
PVAIIATILGYLVGIFVKQDPIKYQQE
HHHHHHHHHHHHHHHHHCCCCCCCCCH
>Mature Secondary Structure
MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFVLTGIGLPLLGVIVGAL
CCCCCEEHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHC
DKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSFEMTITPIIHSNSSIALFI
CCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCHHHHH
FTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMIIKGYLDFSGNSAGKGNEALYH
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEE
SNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGITKTNQIFKQTLTAGLIAAVALI
CCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
FIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLTTMASTGFGSFGKYLLGIIVALACL
HHHHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TTACGLIVAVSEYFHRIVPKVSYKAFVLVFILMSFIIANQGLNAVISMSIPVLSIVYPVA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
ITVVLLILIAKFIPTKRISQQIPVIIVFILSIFSVISKLGWLKINFIESLPLRAYSLEWF
HHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEHHHCCCCCEEECCHH
PVAIIATILGYLVGIFVKQDPIKYQQE
HHHHHHHHHHHHHHHHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA