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The map label for this gene is leuA [H]

Identifier: 161486723

GI number: 161486723

Start: 266152

End: 267897

Strand: Reverse

Name: leuA [H]

Synonym: cg0303

Alternate gene names: 161486723

Gene position: 267897-266152 (Counterclockwise)

Preceding gene: 62389147

Following gene: 62389145

Centisome position: 8.16

GC content: 57.9

Gene sequence:

>1746_bases
ATGCCAGTTAACCGCTACATGCCTTTCGAGGTTGAGGTAGAAGATATTTCTCTGCCGGACCGCACTTGGCCAGATAAAAA
AATCACCGTTGCACCTCAGTGGTGTGCTGTTGACCTGCGTGACGGCAACCAGGCTCTGATTGATCCGATGTCTCCTGAGC
GTAAGCGCCGCATGTTTGAGCTGCTGGTTCAGATGGGCTTCAAAGAAATCGAGGTCGGTTTCCCTTCAGCTTCCCAGACT
GATTTTGATTTCGTTCGTGAGATCATCGAAAAGGGCATGATCCCTGACGATGTCACCATTCAGGTTCTGGTTCAGGCTCG
TGAGCACCTGATTCGCCGTACTTTTGAAGCTTGCGAAGGCGCAAAAAACGTTATCGTGCACTTCTACAACTCCACCTCCA
TCCTGCAGCGCAACGTGGTGTTCCGCATGGACAAGGTGCAGGTGAAGAAGCTGGCTACCGATGCCGCTGAACTAATCAAG
ACCATCGCTCAGGATTACCCAGACACCAACTGGCGCTGGCAGTACTCCCCTGAGTCCTTCACCGGCACTGAGGTTGAGTA
CGCCAAGGAAGTTGTGGACGCAGTTGTTGAGGTCATGGATCCAACTCCTGAGAACCCAATGATCATCAACCTGCCTTCCA
CCGTTGAGATGATCACCCCTAACGTTTACGCAGACTCCATTGAATGGATGCACCGCAATCTAAACCGTCGTGATTCCATT
ATCCTGTCCCTGCACCCGCACAATGACCGTGGCACCGGCGTTGGCGCAGCTGAGCTGGGCTACATGGCTGGCGCTGACCG
CATCGAAGGCTGCCTGTTCGGCAACGGCGAGCGCACCGGCAACGTCTGCCTGGTCACCCTGGCACTGAACATGCTGACCC
AGGGCGTTGACCCTCAGCTGGACTTCACCGATATACGCCAGATCCGCAGCACCGTTGAATACTGCAACCAGCTGCGCGTT
CCTGAGCGCCACCCATACGGCGGTGACCTGGTCTTCACCGCTTTCTCCGGTTCCCACCAGGACGCTGTGAACAAGGGTCT
GGACGCCATGGCTGCCAAGGTTCAGCCAGGTGCTAGCTCCACTGAAGTTTCTTGGGAGCAGCTGCGCGACACCGAATGGG
AGGTTCCTTACCTGCCTATCGATCCAAAGGATGTCGGTCGCGACTACGAGGCTGTTATCCGCGTGAACTCCCAGTCCGGC
AAGGGCGGCGTTGCTTACATCATGAAGACCGATCACGGTCTGCAGATCCCTCGCTCCATGCAGGTTGAGTTCTCCACCGT
TGTCCAGAACGTCACCGACGCTGAGGGCGGCGAGGTCAACTCCAAGGCAATGTGGGATATCTTCGCCACCGAGTACCTGG
AGCGCACCGCACCAGTTGAGCAGATCGCGCTGCGCGTCGAGAACGCTCAGACCGAAAACGAGGATGCATCCATCACCGCC
GAGCTCATCCACAACGGCAAGGACGTCACCGTCGATGGCCGCGGCAACGGCCCACTGGCCGCTTACGCCAACGCGCTGGA
GAAGCTGGGCATCGACGTTGAGATCCAGGAATACAACCAGCACGCCCGCACCTCGGGCGACGATGCAGAAGCAGCCGCCT
ACGTGCTGGCTGAGGTCAACGGCCGCAAGGTCTGGGGCGTCGGCATCGCTGGCTCCATCACCTACGCTTCGCTGAAGGCA
GTGACCTCCGCCGTAAACCGCGCGCTGGACGTCAACCACGAGGCAGTCCTGGCTGGCGGCGTTTAA

Upstream 100 bases:

>100_bases
TCCTAACGATGCATTCATCTCCGCACCTGCCAAGATCGAAACCCCAGTTGGGCCTCGCAACGAAGGCCAGCCAGCATGGA
ATAAGCAGCGTGGCTCCTCA

Downstream 100 bases:

>100_bases
GCTTTACGACGCCTCCCCCTAGGCTCTACAAACCGGTGGCAAGAATTCCACGATGTTGAAAATTCTTGCCACCGGTTTCG
TGGGTGATAGGAATATAGAG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MPVNRYMPFEVEVEDISLPDRTWPDKKITVAPQWCAVDLRDGNQALIDPMSPERKRRMFELLVQMGFKEIEVGFPSASQT
DFDFVREIIEKGMIPDDVTIQVLVQAREHLIRRTFEACEGAKNVIVHFYNSTSILQRNVVFRMDKVQVKKLATDAAELIK
TIAQDYPDTNWRWQYSPESFTGTEVEYAKEVVDAVVEVMDPTPENPMIINLPSTVEMITPNVYADSIEWMHRNLNRRDSI
ILSLHPHNDRGTGVGAAELGYMAGADRIEGCLFGNGERTGNVCLVTLALNMLTQGVDPQLDFTDIRQIRSTVEYCNQLRV
PERHPYGGDLVFTAFSGSHQDAVNKGLDAMAAKVQPGASSTEVSWEQLRDTEWEVPYLPIDPKDVGRDYEAVIRVNSQSG
KGGVAYIMKTDHGLQIPRSMQVEFSTVVQNVTDAEGGEVNSKAMWDIFATEYLERTAPVEQIALRVENAQTENEDASITA
ELIHNGKDVTVDGRGNGPLAAYANALEKLGIDVEIQEYNQHARTSGDDAEAAAYVLAEVNGRKVWGVGIAGSITYASLKA
VTSAVNRALDVNHEAVLAGGV

Sequences:

>Translated_581_residues
MPVNRYMPFEVEVEDISLPDRTWPDKKITVAPQWCAVDLRDGNQALIDPMSPERKRRMFELLVQMGFKEIEVGFPSASQT
DFDFVREIIEKGMIPDDVTIQVLVQAREHLIRRTFEACEGAKNVIVHFYNSTSILQRNVVFRMDKVQVKKLATDAAELIK
TIAQDYPDTNWRWQYSPESFTGTEVEYAKEVVDAVVEVMDPTPENPMIINLPSTVEMITPNVYADSIEWMHRNLNRRDSI
ILSLHPHNDRGTGVGAAELGYMAGADRIEGCLFGNGERTGNVCLVTLALNMLTQGVDPQLDFTDIRQIRSTVEYCNQLRV
PERHPYGGDLVFTAFSGSHQDAVNKGLDAMAAKVQPGASSTEVSWEQLRDTEWEVPYLPIDPKDVGRDYEAVIRVNSQSG
KGGVAYIMKTDHGLQIPRSMQVEFSTVVQNVTDAEGGEVNSKAMWDIFATEYLERTAPVEQIALRVENAQTENEDASITA
ELIHNGKDVTVDGRGNGPLAAYANALEKLGIDVEIQEYNQHARTSGDDAEAAAYVLAEVNGRKVWGVGIAGSITYASLKA
VTSAVNRALDVNHEAVLAGGV
>Mature_580_residues
PVNRYMPFEVEVEDISLPDRTWPDKKITVAPQWCAVDLRDGNQALIDPMSPERKRRMFELLVQMGFKEIEVGFPSASQTD
FDFVREIIEKGMIPDDVTIQVLVQAREHLIRRTFEACEGAKNVIVHFYNSTSILQRNVVFRMDKVQVKKLATDAAELIKT
IAQDYPDTNWRWQYSPESFTGTEVEYAKEVVDAVVEVMDPTPENPMIINLPSTVEMITPNVYADSIEWMHRNLNRRDSII
LSLHPHNDRGTGVGAAELGYMAGADRIEGCLFGNGERTGNVCLVTLALNMLTQGVDPQLDFTDIRQIRSTVEYCNQLRVP
ERHPYGGDLVFTAFSGSHQDAVNKGLDAMAAKVQPGASSTEVSWEQLRDTEWEVPYLPIDPKDVGRDYEAVIRVNSQSGK
GGVAYIMKTDHGLQIPRSMQVEFSTVVQNVTDAEGGEVNSKAMWDIFATEYLERTAPVEQIALRVENAQTENEDASITAE
LIHNGKDVTVDGRGNGPLAAYANALEKLGIDVEIQEYNQHARTSGDDAEAAAYVLAEVNGRKVWGVGIAGSITYASLKAV
TSAVNRALDVNHEAVLAGGV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=549, Percent_Identity=25.6830601092896, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324225, Length=578, Percent_Identity=46.3667820069204, Blast_Score=532, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6324682, Length=558, Percent_Identity=46.9534050179211, Blast_Score=525, Evalue=1e-150,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 64467; Mature: 64336

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVNRYMPFEVEVEDISLPDRTWPDKKITVAPQWCAVDLRDGNQALIDPMSPERKRRMFE
CCCCCCCCCEEEEECCCCCCCCCCCCEEEECCCEEEEEECCCCEEEECCCCHHHHHHHHH
LLVQMGFKEIEVGFPSASQTDFDFVREIIEKGMIPDDVTIQVLVQAREHLIRRTFEACEG
HHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
AKNVIVHFYNSTSILQRNVVFRMDKVQVKKLATDAAELIKTIAQDYPDTNWRWQYSPESF
CCEEEEEEECCHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
TGTEVEYAKEVVDAVVEVMDPTPENPMIINLPSTVEMITPNVYADSIEWMHRNLNRRDSI
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCCE
ILSLHPHNDRGTGVGAAELGYMAGADRIEGCLFGNGERTGNVCLVTLALNMLTQGVDPQL
EEEEECCCCCCCCCCHHHHHHHCCCHHHCEEEECCCCCCCCEEEHHHHHHHHHCCCCCCC
DFTDIRQIRSTVEYCNQLRVPERHPYGGDLVFTAFSGSHQDAVNKGLDAMAAKVQPGASS
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHCHHHHHHHCCCCCCC
TEVSWEQLRDTEWEVPYLPIDPKDVGRDYEAVIRVNSQSGKGGVAYIMKTDHGLQIPRSM
CCCCHHHHCCCCCCCCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCC
QVEFSTVVQNVTDAEGGEVNSKAMWDIFATEYLERTAPVEQIALRVENAQTENEDASITA
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEH
ELIHNGKDVTVDGRGNGPLAAYANALEKLGIDVEIQEYNQHARTSGDDAEAAAYVLAEVN
HHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEHHHHHHHCCCCCCHHHHEEEEEECC
GRKVWGVGIAGSITYASLKAVTSAVNRALDVNHEAVLAGGV
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCC
>Mature Secondary Structure 
PVNRYMPFEVEVEDISLPDRTWPDKKITVAPQWCAVDLRDGNQALIDPMSPERKRRMFE
CCCCCCCCEEEEECCCCCCCCCCCCEEEECCCEEEEEECCCCEEEECCCCHHHHHHHHH
LLVQMGFKEIEVGFPSASQTDFDFVREIIEKGMIPDDVTIQVLVQAREHLIRRTFEACEG
HHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
AKNVIVHFYNSTSILQRNVVFRMDKVQVKKLATDAAELIKTIAQDYPDTNWRWQYSPESF
CCEEEEEEECCHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
TGTEVEYAKEVVDAVVEVMDPTPENPMIINLPSTVEMITPNVYADSIEWMHRNLNRRDSI
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCCE
ILSLHPHNDRGTGVGAAELGYMAGADRIEGCLFGNGERTGNVCLVTLALNMLTQGVDPQL
EEEEECCCCCCCCCCHHHHHHHCCCHHHCEEEECCCCCCCCEEEHHHHHHHHHCCCCCCC
DFTDIRQIRSTVEYCNQLRVPERHPYGGDLVFTAFSGSHQDAVNKGLDAMAAKVQPGASS
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHCHHHHHHHCCCCCCC
TEVSWEQLRDTEWEVPYLPIDPKDVGRDYEAVIRVNSQSGKGGVAYIMKTDHGLQIPRSM
CCCCHHHHCCCCCCCCCCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCC
QVEFSTVVQNVTDAEGGEVNSKAMWDIFATEYLERTAPVEQIALRVENAQTENEDASITA
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEH
ELIHNGKDVTVDGRGNGPLAAYANALEKLGIDVEIQEYNQHARTSGDDAEAAAYVLAEVN
HHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEHHHHHHHCCCCCCHHHHEEEEEECC
GRKVWGVGIAGSITYASLKAVTSAVNRALDVNHEAVLAGGV
CCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA