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The map label for this gene is garR

Identifier: 161486431

GI number: 161486431

Start: 3254479

End: 3255369

Strand: Reverse

Name: garR

Synonym: S3377

Alternate gene names: 161486431

Gene position: 3255369-3254479 (Counterclockwise)

Preceding gene: 30064466

Following gene: 161486432

Centisome position: 70.78

GC content: 52.97

Gene sequence:

>891_bases
ATGACTATGAAAGTTGGTTTTATTGGCCTGGGGATTATGGGTAAACCAATGAGTAAAAACCTTCTGAAAGCAGGTTACTC
GCTGGTGGTTGCTGACCGTAACCCAGAAGCTATTGCTGACGTGATTGCTGCAGGTGCAGAAACAGCCTCTACGGCTAAAG
CGATCGCTGAACAGTGCGACGTCATCATAACCATGCTGCCAAACTCCCCTCATGTGAAAGAGGTGGCGCTGGGTGAGAAT
GGCATTATTGAAGGCGCGAAGCCAGGTACGGTATTGATCGATATGAGTTCTATCGCACCGCTGGCAAGCCGTGAAATCAG
CGAAGCGCTGAAAGCGAAAGGCATTGATATGCTGGATGCTCCGGTGAGCGGCGGTGAACCGAAAGCCATCGACGGTACGC
TGTCAGTGATGGTGGGCGGCGACAAGGCTATTTTCGACAAATACTATGATTTGATGAAAGCAATGGCGGGTTCCGTGGTG
CATACCGGGGCAATTGGTGCAGGTAACGTCACCAAACTGGCGAATCAGGTCATTGTGGCGCTGAATATTGCCGCGATGTC
AGAAGCGTTAACGCTGGCAACTAAAGCGGGCGTTAACCCGGACCTGGTGTATCAGGCAATTCGCGGTGGACTGGCGGGCA
GTACCGTGCTGGATGCCAAAGCGCCGATGGTGATGGACCGCAACTTCAAGCCGGGCTTCCGTATTGATCTGCATATTAAG
GATCTGGCAAATGCGCTGGATACTTCCCACGGCGTCGGCGCGCAACTGCCGCTCACAGCTGCGGTTATGGAGATGATGCA
GGCACTGCGAGCAGATGGTTTAGGAACGGCGGATCATAGCGCCCTGGTGTGCTACTACGAAAAACTGGCGAAAGTCGAAG
TTACTCGTTAA

Upstream 100 bases:

>100_bases
TGGCTGTCGGCAGCGATCTCGGCGTCTTCCGCTCTGCCACTCATAAACTGGCTGATACCTTTAAAAAATAACCACCACCG
CAACAAGAGAGATGATTGAT

Downstream 100 bases:

>100_bases
TGACGTCGTGCCCGGATGGCGTATTGCAATCCGGGCCTGGAATCCAGGCCGAATAAGAGAAACGCCCTCCGGCGTCATGT
GGTAACAGGCATAGGTATGA

Product: tartronate semialdehyde reductase

Products: NA

Alternate protein names: Tartronate semialdehyde reductase; TSAR [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVIITMLPNSPHVKEVALGEN
GIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIK
DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALVCYYEKLAKVEVTR

Sequences:

>Translated_296_residues
MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVIITMLPNSPHVKEVALGEN
GIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIK
DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALVCYYEKLAKVEVTR
>Mature_295_residues
TMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENG
IIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVH
TGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKD
LANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALVCYYEKLAKVEVTR

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=35.0515463917526, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI40556376, Length=282, Percent_Identity=28.7234042553192, Blast_Score=135, Evalue=3e-32,
Organism=Escherichia coli, GI145693186, Length=294, Percent_Identity=99.3197278911565, Blast_Score=582, Evalue=1e-168,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=44.2906574394464, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1790315, Length=281, Percent_Identity=36.6548042704626, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1789092, Length=289, Percent_Identity=33.5640138408304, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17557316, Length=275, Percent_Identity=33.0909090909091, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24655230, Length=284, Percent_Identity=32.3943661971831, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922568, Length=284, Percent_Identity=32.3943661971831, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI28574115, Length=280, Percent_Identity=25.7142857142857, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183
- InterPro:   IPR006398 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.60 [H]

Molecular weight: Translated: 30630; Mature: 30499

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCD
CEEEEEEEEECCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCC
VIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDA
EEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHCCCCEECC
PVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGAIGAGNVTKLANQVIVA
CCCCCCCCEECCEEEEEECCCHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHE
LNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIK
EHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCEEECCCCCCCCEEEEEHH
DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALVCYYEKLAKVEVTR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEECCC
>Mature Secondary Structure 
TMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCD
EEEEEEEEECCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHCC
VIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDA
EEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHHHCCCCEECC
PVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGAIGAGNVTKLANQVIVA
CCCCCCCCEECCEEEEEECCCHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHE
LNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIK
EHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCEEECCCCCCCCEEEEEHH
DLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALVCYYEKLAKVEVTR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]