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The map label for this gene is imp [H]

Identifier: 161484744

GI number: 161484744

Start: 4090134

End: 4092476

Strand: Direct

Name: imp [H]

Synonym: y3680

Alternate gene names: 161484744

Gene position: 4090134-4092476 (Clockwise)

Preceding gene: 22127552

Following gene: 22127555

Centisome position: 88.9

GC content: 47.84

Gene sequence:

>2343_bases
ATGAAAAAAAGATTCCCAACACTGCTGGCCACATTGATTTGGACGGCACTATATAGTCAACACACACTGGCAGACCTTGC
GGAGCAATGTATGCTCGGTGTCCCAACCTATGATCAACCACTGGTCACAGGCGATCCGAATCAACTGCCGGTCCGTATTA
ATGCGGATAAAACCGAAGCAAACTATCCGGATAATGCCTTGTTTACTGGCAATGTTATTGTTCAGCAAGGCAATAGCACC
CTAACAGCGAACCAAGTGGAACTGACACAGGTACAGAAACCCGGTGAAGTCATTCCCCTTCGTACCGTTACCGCCACCGG
CGATGTCAACTACGACGATCCGCAAATCAAGCTGAAAGGACCAAAAGGTTGGTCAAATCTGAACACCAAAGATACTGATA
TGGACAAAGGTAAATATCAGATGGTGGGCCGTCAGGGCCGTGGTGATGCAGATTTGATGAAATTGCGCGATCAGAGCCGC
TACACCATTTTGAAAAACGGCACATTTACCTCTTGCCTACCCGGAGATAACAGTTGGAGCGTGGTTGGCTCAGAGGTCAT
TCATGACCGCGAAGAACAAGTGGTTGAAGTTTGGAATGCTCGTTTCAAGATTGGCAAAGTCCCGGTGTTTTACAGCCCTT
ATATGCAATTGCCAGTTGGCGATAAACGCCGTTCGGGGTTCTTGATCCCAAATGCGAAATTTACCAGTAACAATGGTTTT
GAGTTTTTACTCCCTTATTACTGGAATATTGCCCCCAACTTTGATGCCACCATTACGCCACACTATATGGAAAGGCGCGG
GTTGCAATGGCAAAACGAATTCCGCTATTTGCTGGCGCCGGGTTCAGGCACCATGGCATTAGACTGGTTGCCCAATGACC
GTATCTATACGGGGCCTGATGGCACAGATAAAAATGCCACCCGCTGGTTATACTACTGGGGCCATTCCGGTGTAATGGAT
CAAGTTTGGCGCTTCAACATCAACTATACCCGTGTCAGCGACCCCGCTTACTTTACGGATTTAACCTCTCAGTACGGTTC
GACAACCGATGGCTACGCGACACAAATATTCACCGCCGGTTATGCCAATGAAAACTGGAATGCGACGCTGTCTTCCAAGC
AGTTCCAGGTATTTACTGCTGCAGGTAACAGTAATGCCTACCGTGCCCAGCCACAGTTGGACATGAACTACTACAAAAAC
GATGTTGGTCCATTTGATATGCATGTCTATGGTCAGGCGGCCAAATTCACCAGTGTCAATCCAACTAACCCGGAAGCCAG
CCGTTTCCATATTGAACCTACGGTTAACCTGCCCTTATCCAATAGCTGGGGAAGCATAAATACAGAAGCCAAACTGTTGG
CCACCCACTATCAACAGGATATTCCGGCCAGTTTTGCTGACAATGCTAGCAACCCCAAACTGAAAGACTCAGTAAACCGG
GTCCTACCACAGTTTAAAGTGGATGGTAAGGTGGTATTCGATAGGTCAATGGACTGGGCGACGGGCTTTACGCAGACATT
GGAGCCTCGCGCACAGTATCTCTACGTCCCTTACCGGAACCAAGATGATATCTATATATACGACACCACGTTGATGCAGT
CGGATTACTCTGGTCTGTTCCGTGATCGTACCTATAGTGGCTTGGACCGTATTGCATCGGCTAATCAGGTATCTACCGGT
TTAACCTCTCGCATATATGATGATGCTCGGGTTGAACGTTTTAATGTCTCTGTAGGTCAAATCTATTACTTCAGTCGATC
ACGCACAGGTAATACCGAAGCCATCGATAACAGTAACGCGACAGGAAGCCTGGTTTGGGCTGGTGATACTTTTTGGCGGA
TCAACGATCAACTGGGCCTGAAGGGCGGTGCACAGTATGATACGCGGCTGGGAAGTTTGACCTTAGGTAACGCTATAATG
GAATATAGAAAAGACGCTGATCGTATGATTCAATTGAATTATCGCTACGCGAGCCCAAAATATATTCAGGCTGCTGTCCC
TAAAGTATACAACCCTGACTACCAGCAAGGCATCTCTCAGGTAGGTACCACGGCCAGTTGGCCAATTGCTGACCGCTGGG
CGATCGTCGGGGCGTATTATTACGATACCAAAGCCAAACAACCTGCCAGCCAGTTAGTTGGCTTGCAATACAACACCTGT
TGTTGGGCGGTAAACCTTGGCTATGAACGCAAAATAACTGGCTGGAATGCACAAGGCCAAACCAGTAAGTACGACAACAA
AATAGGCTTTAACATCGAATTACGTGGTTTGAGCGGTGGTCATAGCCTTGGTACTGCTCAAATGCTGAACTCGGGCATTC
TGCCTTATCAAAGTGCATTCTGA

Upstream 100 bases:

>100_bases
AAACGGCCAGATTTAACACTAGCGCAACTGTGATGAGTGATATAGTCTCAAACCGTTTGCCGGCATGATGCCCCCCGATG
ACGGAACACCAAAACCACGT

Downstream 100 bases:

>100_bases
TACTGTTTAACACACTGAATACAGTGAATCTTAACCCGCGATAGCGGATTACATAAATGGAAAAGGTATGAAGAACTGGA
GAACGCTTATTCTCGGATTG

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 780; Mature: 780

Protein sequence:

>780_residues
MKKRFPTLLATLIWTALYSQHTLADLAEQCMLGVPTYDQPLVTGDPNQLPVRINADKTEANYPDNALFTGNVIVQQGNST
LTANQVELTQVQKPGEVIPLRTVTATGDVNYDDPQIKLKGPKGWSNLNTKDTDMDKGKYQMVGRQGRGDADLMKLRDQSR
YTILKNGTFTSCLPGDNSWSVVGSEVIHDREEQVVEVWNARFKIGKVPVFYSPYMQLPVGDKRRSGFLIPNAKFTSNNGF
EFLLPYYWNIAPNFDATITPHYMERRGLQWQNEFRYLLAPGSGTMALDWLPNDRIYTGPDGTDKNATRWLYYWGHSGVMD
QVWRFNINYTRVSDPAYFTDLTSQYGSTTDGYATQIFTAGYANENWNATLSSKQFQVFTAAGNSNAYRAQPQLDMNYYKN
DVGPFDMHVYGQAAKFTSVNPTNPEASRFHIEPTVNLPLSNSWGSINTEAKLLATHYQQDIPASFADNASNPKLKDSVNR
VLPQFKVDGKVVFDRSMDWATGFTQTLEPRAQYLYVPYRNQDDIYIYDTTLMQSDYSGLFRDRTYSGLDRIASANQVSTG
LTSRIYDDARVERFNVSVGQIYYFSRSRTGNTEAIDNSNATGSLVWAGDTFWRINDQLGLKGGAQYDTRLGSLTLGNAIM
EYRKDADRMIQLNYRYASPKYIQAAVPKVYNPDYQQGISQVGTTASWPIADRWAIVGAYYYDTKAKQPASQLVGLQYNTC
CWAVNLGYERKITGWNAQGQTSKYDNKIGFNIELRGLSGGHSLGTAQMLNSGILPYQSAF

Sequences:

>Translated_780_residues
MKKRFPTLLATLIWTALYSQHTLADLAEQCMLGVPTYDQPLVTGDPNQLPVRINADKTEANYPDNALFTGNVIVQQGNST
LTANQVELTQVQKPGEVIPLRTVTATGDVNYDDPQIKLKGPKGWSNLNTKDTDMDKGKYQMVGRQGRGDADLMKLRDQSR
YTILKNGTFTSCLPGDNSWSVVGSEVIHDREEQVVEVWNARFKIGKVPVFYSPYMQLPVGDKRRSGFLIPNAKFTSNNGF
EFLLPYYWNIAPNFDATITPHYMERRGLQWQNEFRYLLAPGSGTMALDWLPNDRIYTGPDGTDKNATRWLYYWGHSGVMD
QVWRFNINYTRVSDPAYFTDLTSQYGSTTDGYATQIFTAGYANENWNATLSSKQFQVFTAAGNSNAYRAQPQLDMNYYKN
DVGPFDMHVYGQAAKFTSVNPTNPEASRFHIEPTVNLPLSNSWGSINTEAKLLATHYQQDIPASFADNASNPKLKDSVNR
VLPQFKVDGKVVFDRSMDWATGFTQTLEPRAQYLYVPYRNQDDIYIYDTTLMQSDYSGLFRDRTYSGLDRIASANQVSTG
LTSRIYDDARVERFNVSVGQIYYFSRSRTGNTEAIDNSNATGSLVWAGDTFWRINDQLGLKGGAQYDTRLGSLTLGNAIM
EYRKDADRMIQLNYRYASPKYIQAAVPKVYNPDYQQGISQVGTTASWPIADRWAIVGAYYYDTKAKQPASQLVGLQYNTC
CWAVNLGYERKITGWNAQGQTSKYDNKIGFNIELRGLSGGHSLGTAQMLNSGILPYQSAF
>Mature_780_residues
MKKRFPTLLATLIWTALYSQHTLADLAEQCMLGVPTYDQPLVTGDPNQLPVRINADKTEANYPDNALFTGNVIVQQGNST
LTANQVELTQVQKPGEVIPLRTVTATGDVNYDDPQIKLKGPKGWSNLNTKDTDMDKGKYQMVGRQGRGDADLMKLRDQSR
YTILKNGTFTSCLPGDNSWSVVGSEVIHDREEQVVEVWNARFKIGKVPVFYSPYMQLPVGDKRRSGFLIPNAKFTSNNGF
EFLLPYYWNIAPNFDATITPHYMERRGLQWQNEFRYLLAPGSGTMALDWLPNDRIYTGPDGTDKNATRWLYYWGHSGVMD
QVWRFNINYTRVSDPAYFTDLTSQYGSTTDGYATQIFTAGYANENWNATLSSKQFQVFTAAGNSNAYRAQPQLDMNYYKN
DVGPFDMHVYGQAAKFTSVNPTNPEASRFHIEPTVNLPLSNSWGSINTEAKLLATHYQQDIPASFADNASNPKLKDSVNR
VLPQFKVDGKVVFDRSMDWATGFTQTLEPRAQYLYVPYRNQDDIYIYDTTLMQSDYSGLFRDRTYSGLDRIASANQVSTG
LTSRIYDDARVERFNVSVGQIYYFSRSRTGNTEAIDNSNATGSLVWAGDTFWRINDQLGLKGGAQYDTRLGSLTLGNAIM
EYRKDADRMIQLNYRYASPKYIQAAVPKVYNPDYQQGISQVGTTASWPIADRWAIVGAYYYDTKAKQPASQLVGLQYNTC
CWAVNLGYERKITGWNAQGQTSKYDNKIGFNIELRGLSGGHSLGTAQMLNSGILPYQSAF

Specific function: Together with lptE, is involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=786, Percent_Identity=66.5394402035623, Blast_Score=1102, Evalue=0.0,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 87910; Mature: 87910

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRFPTLLATLIWTALYSQHTLADLAEQCMLGVPTYDQPLVTGDPNQLPVRINADKTEA
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEEEECCCCCC
NYPDNALFTGNVIVQQGNSTLTANQVELTQVQKPGEVIPLRTVTATGDVNYDDPQIKLKG
CCCCCEEEECEEEEECCCCEEEECEEEEEEECCCCCEEEEEEEEECCCCCCCCCEEEEEC
PKGWSNLNTKDTDMDKGKYQMVGRQGRGDADLMKLRDQSRYTILKNGTFTSCLPGDNSWS
CCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCEEEEECCCEEEECCCCCCCE
VVGSEVIHDREEQVVEVWNARFKIGKVPVFYSPYMQLPVGDKRRSGFLIPNAKFTSNNGF
EECHHHHHCHHHHHHHHHCCEEEECCCEEEECCEEECCCCCCCCCCEECCCCEEECCCCC
EFLLPYYWNIAPNFDATITPHYMERRGLQWQNEFRYLLAPGSGTMALDWLPNDRIYTGPD
EEEEEEEEECCCCCCCEECCHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCCCEEECCC
GTDKNATRWLYYWGHSGVMDQVWRFNINYTRVSDPAYFTDLTSQYGSTTDGYATQIFTAG
CCCCCCEEEEEEECCCCHHHHHEEEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEEC
YANENWNATLSSKQFQVFTAAGNSNAYRAQPQLDMNYYKNDVGPFDMHVYGQAAKFTSVN
CCCCCCCEEECCCEEEEEEEECCCCCEECCCCCCHHHHHCCCCCEEEEEEECCEEECCCC
PTNPEASRFHIEPTVNLPLSNSWGSINTEAKLLATHYQQDIPASFADNASNPKLKDSVNR
CCCCCCCEEEECEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHH
VLPQFKVDGKVVFDRSMDWATGFTQTLEPRAQYLYVPYRNQDDIYIYDTTLMQSDYSGLF
HCCCEECCCEEEEECCCCCCCCCHHHCCCCCCEEEEEECCCCCEEEEEEEEECCCCCHHH
RDRTYSGLDRIASANQVSTGLTSRIYDDARVERFNVSVGQIYYFSRSRTGNTEAIDNSNA
HCCCHHHHHHHHCCCHHHHCHHHHHHCCCCEEEEECEEEEEEEEECCCCCCCCCCCCCCC
TGSLVWAGDTFWRINDQLGLKGGAQYDTRLGSLTLGNAIMEYRKDADRMIQLNYRYASPK
CEEEEECCCEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCEEEEEEEEECCCC
YIQAAVPKVYNPDYQQGISQVGTTASWPIADRWAIVGAYYYDTKAKQPASQLVGLQYNTC
HHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHCCCEECCE
CWAVNLGYERKITGWNAQGQTSKYDNKIGFNIELRGLSGGHSLGTAQMLNSGILPYQSAF
EEEEECCCEEEECCCCCCCCCCCCCCEECEEEEEEECCCCCCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKRFPTLLATLIWTALYSQHTLADLAEQCMLGVPTYDQPLVTGDPNQLPVRINADKTEA
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEEEECCCCCC
NYPDNALFTGNVIVQQGNSTLTANQVELTQVQKPGEVIPLRTVTATGDVNYDDPQIKLKG
CCCCCEEEECEEEEECCCCEEEECEEEEEEECCCCCEEEEEEEEECCCCCCCCCEEEEEC
PKGWSNLNTKDTDMDKGKYQMVGRQGRGDADLMKLRDQSRYTILKNGTFTSCLPGDNSWS
CCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCEEEEECCCEEEECCCCCCCE
VVGSEVIHDREEQVVEVWNARFKIGKVPVFYSPYMQLPVGDKRRSGFLIPNAKFTSNNGF
EECHHHHHCHHHHHHHHHCCEEEECCCEEEECCEEECCCCCCCCCCEECCCCEEECCCCC
EFLLPYYWNIAPNFDATITPHYMERRGLQWQNEFRYLLAPGSGTMALDWLPNDRIYTGPD
EEEEEEEEECCCCCCCEECCHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCCCEEECCC
GTDKNATRWLYYWGHSGVMDQVWRFNINYTRVSDPAYFTDLTSQYGSTTDGYATQIFTAG
CCCCCCEEEEEEECCCCHHHHHEEEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEEC
YANENWNATLSSKQFQVFTAAGNSNAYRAQPQLDMNYYKNDVGPFDMHVYGQAAKFTSVN
CCCCCCCEEECCCEEEEEEEECCCCCEECCCCCCHHHHHCCCCCEEEEEEECCEEECCCC
PTNPEASRFHIEPTVNLPLSNSWGSINTEAKLLATHYQQDIPASFADNASNPKLKDSVNR
CCCCCCCEEEECEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHH
VLPQFKVDGKVVFDRSMDWATGFTQTLEPRAQYLYVPYRNQDDIYIYDTTLMQSDYSGLF
HCCCEECCCEEEEECCCCCCCCCHHHCCCCCCEEEEEECCCCCEEEEEEEEECCCCCHHH
RDRTYSGLDRIASANQVSTGLTSRIYDDARVERFNVSVGQIYYFSRSRTGNTEAIDNSNA
HCCCHHHHHHHHCCCHHHHCHHHHHHCCCCEEEEECEEEEEEEEECCCCCCCCCCCCCCC
TGSLVWAGDTFWRINDQLGLKGGAQYDTRLGSLTLGNAIMEYRKDADRMIQLNYRYASPK
CEEEEECCCEEEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCEEEEEEEEECCCC
YIQAAVPKVYNPDYQQGISQVGTTASWPIADRWAIVGAYYYDTKAKQPASQLVGLQYNTC
HHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHCCCEECCE
CWAVNLGYERKITGWNAQGQTSKYDNKIGFNIELRGLSGGHSLGTAQMLNSGILPYQSAF
EEEEECCCEEEECCCCCCCCCCCCCCEECEEEEEEECCCCCCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA