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The map label for this gene is pheS [H]

Identifier: 161378151

GI number: 161378151

Start: 623951

End: 624994

Strand: Direct

Name: pheS [H]

Synonym: SPy_0768

Alternate gene names: 161378151

Gene position: 623951-624994 (Clockwise)

Preceding gene: 15674813

Following gene: 15674815

Centisome position: 33.68

GC content: 41.86

Gene sequence:

>1044_bases
ATGGATTTACAAGCACAATTAGAGGAATTAAAGACAAAAACATTGGAGACCTTACAGTCTTTGACGGGTAATCATACCAA
AGAACTGCAAGATTTGCGCGTGGCTGTTCTTGGGAAAAAGGGATCTTTGACAGAATTACTAAAGGGGCTAAAAGACTTGT
CTAATGACTTGCGTCCTGTGGTTGGTAAACAGGTGAACGAAGTTCGTGACTTATTGACAAAAGCCTTTGAAGAACAGGCT
AAAATTGTAGAGGCTGCTAAAATTCAGGCCCAACTTGATGCTGAAAGCATTGATGTGACTTTACCGGGTCGTCAAATGAC
TCTGGGGCATCGTCATGTGTTAACACAAACCAGTGAAGAAATCGAAGATATTTTCTTAGGAATGGGTTTTCAGATTGTTG
ATGGCTTTGAGGTGGAAAAAGATTATTACAACTTTGAGCGCATGAATTTGCCAAAGGATCACCCTGCCCGTGACATGCAA
GATACGTTTTATATCACAGAAGAAATCTTGTTACGAACTCACACCAGTCCTGTGCAGGCCCGTACTCTTGATCAACATGA
TTTTTCAAAAGGGCCTTTAAAAATGGTTTCTCCAGGTCGTGTTTTCCGTCGTGATACTGATGACGCTACCCACTCACATC
AATTCCATCAAATTGAAGGTTTGGTTGTGGGCAAAAATATTTCCATGGGAGACCTCAAAGGGACCCTTGAAATGATCATC
AAGAAGATGTTCGGTGACGAACGTAGCATTCGTTTGCGCCCGTCATATTTCCCGTTCACAGAACCATCTGTTGAAGTGGA
TGTTTCTTGCTTTAAATGTGGTGGGAAAGGCTGTAATGTCTGCAAGAAAACAGGATGGATTGAAATTCTTGGTGCTGGTA
TGGTTCACCCAAGTGTCTTGGAAATGTCAGGTGTTGATGCAAAAGAGTATTCAGGCTTTGCCTTTGGTTTAGGTCAAGAG
CGGATTGCCATGTTGCGTTATGGCATTAATGACATTCGTGGTTTCTATCAAGGCGACCAACGTTTCTCTGAACAATTTAA
CTAA

Upstream 100 bases:

>100_bases
TGATAACGTCAGATAAAAACGGATGGTACCGCGTGTCAACGCTCCGCTTATCAAGTGGAGGAGTGGCATGCTTTTTTCAT
CGCCAAAAGAAGGGAAAACT

Downstream 100 bases:

>100_bases
AACGAGTCGTTTGAAGACCTCTGTCAGCTATTTTATCTAAGATTGAGTGAAAGTTGATAGGTAATGATAATAGGTTAATT
GTCTAAGGGGAAAGTCCCTC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLTELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQA
KIVEAAKIQAQLDAESIDVTLPGRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQIEGLVVGKNISMGDLKGTLEMII
KKMFGDERSIRLRPSYFPFTEPSVEVDVSCFKCGGKGCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQE
RIAMLRYGINDIRGFYQGDQRFSEQFN

Sequences:

>Translated_347_residues
MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLTELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQA
KIVEAAKIQAQLDAESIDVTLPGRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQIEGLVVGKNISMGDLKGTLEMII
KKMFGDERSIRLRPSYFPFTEPSVEVDVSCFKCGGKGCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQE
RIAMLRYGINDIRGFYQGDQRFSEQFN
>Mature_347_residues
MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLTELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQA
KIVEAAKIQAQLDAESIDVTLPGRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQIEGLVVGKNISMGDLKGTLEMII
KKMFGDERSIRLRPSYFPFTEPSVEVDVSCFKCGGKGCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQE
RIAMLRYGINDIRGFYQGDQRFSEQFN

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=268, Percent_Identity=33.2089552238806, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI5729820, Length=292, Percent_Identity=29.7945205479452, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1788007, Length=318, Percent_Identity=45.9119496855346, Blast_Score=295, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI32563657, Length=271, Percent_Identity=32.8413284132841, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17508957, Length=271, Percent_Identity=32.8413284132841, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI32566635, Length=247, Percent_Identity=34.412955465587, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321087, Length=274, Percent_Identity=32.4817518248175, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6325304, Length=349, Percent_Identity=27.5071633237822, Blast_Score=113, Evalue=6e-26,
Organism=Drosophila melanogaster, GI18858079, Length=286, Percent_Identity=31.4685314685315, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI17137424, Length=252, Percent_Identity=28.968253968254, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39179; Mature: 39179

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLTELLKGLKDLSNDLRPV
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHH
VGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLPGRQMTLGHRHVLTQTSEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCHHHHHCCHHH
IEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQDTFYITEEILLRTHTSPVQA
HHHHHHCCCEEEECCEEECHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHH
RTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQIEGLVVGKNISMGDLKGTLEMII
HCCCCCCCCCCCEEECCCCHHEECCCCCCHHCCHHHEECCEEEECCCCHHHHHHHHHHHH
KKMFGDERSIRLRPSYFPFTEPSVEVDVSCFKCGGKGCNVCKKTGWIEILGAGMVHPSVL
HHHHCCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCCCHHHHCCCEEEEECCCCCCHHH
EMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRGFYQGDQRFSEQFN
HHCCCCHHHCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLTELLKGLKDLSNDLRPV
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHH
VGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLPGRQMTLGHRHVLTQTSEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCHHHHHCCHHH
IEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQDTFYITEEILLRTHTSPVQA
HHHHHHCCCEEEECCEEECHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHH
RTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQIEGLVVGKNISMGDLKGTLEMII
HCCCCCCCCCCCEEECCCCHHEECCCCCCHHCCHHHEECCEEEECCCCHHHHHHHHHHHH
KKMFGDERSIRLRPSYFPFTEPSVEVDVSCFKCGGKGCNVCKKTGWIEILGAGMVHPSVL
HHHHCCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCCCHHHHCCCEEEEECCCCCCHHH
EMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRGFYQGDQRFSEQFN
HHCCCCHHHCCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA