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The map label for this gene is hisF

Identifier: 160902484

GI number: 160902484

Start: 1087657

End: 1088415

Strand: Direct

Name: hisF

Synonym: Pmob_1021

Alternate gene names: 160902484

Gene position: 1087657-1088415 (Clockwise)

Preceding gene: 160902483

Following gene: 160902485

Centisome position: 50.13

GC content: 37.81

Gene sequence:

>759_bases
ATGTTGACAAAAAGAATCGTAGCCGCCTTGGATATAAAAGAAGGAAGAGTCGTAAAGGGAGTACAATTTGAGAACATACG
GGACGCGGGAGATCCTGTGGAACTTGCCAAAAAATATGAAAAAGATGGGGTTGATGAGATCGTATTCTTAGATATAACGG
CCTCTAAAGAAAAACGCAATATACTCAAAAATCTTGTTGAGGAAATTGCCAAAGAACTATTCATTCCTTTCACCGTGGGT
GGTGGATTAAAAACAGTCGAACAAATGGTAGAAATAATAAAATGTGGAGCAGATAAAGTGTTCATAAATTCAGCTGCCGT
TGAAAATCCAAATTTAATTAAAGAAAGTTCAAAAATAATAGGGAGCTCCAACGTAGTAGTAGCGATAGATGCAAAAAAAG
ACGTCGAAAGTGAAAAGTATTACGTATACACACACGGAGGAAGTAAAAAAACGGATTTAGACGCTGTTGAGTGGGCTAGA
AAATGTCAAGAGTTGGGAGCAGGGGAACTACTGGTTACTTCTATGAACACCGATGGAGTAAAAAAAGGGTATGACTTGAA
TTTAACCAAACAAATAGTTGATGCTGTAGAAATCCCAGTTATCGCCTCAGGTGGAGCGGGAGAAGTTAAAGATTTCATCG
ATGTTTTTCAAATAGGTGCAGATGCTGCTCTTGCTGCATCCATATTCCATTATGGTATCTACACTGCAAAAGACCTCAAA
ATCCAATTGAAGAAGGTGGGAATAAATGTTAGATTATAA

Upstream 100 bases:

>100_bases
ATTTAAAAAAATTGGAAAATATAGGTTTCGATGGGGCTATAATAGGAAAAGCTCTTTACGAAGGTAAAATCAATTTGCAG
GAGTTAAGGGGTGAACAATA

Downstream 100 bases:

>100_bases
ACAAATAATAGACAAATTAGATTGGGAAAAAAACAATGGCCTCATACCTGTAATAGTTCAAGGTCTCGATGGTGACGTAT
TAACTTTAGGTTATACAGAC

Product: imidazoleglycerol phosphate synthase, cyclase subunit

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLTKRIVAALDIKEGRVVKGVQFENIRDAGDPVELAKKYEKDGVDEIVFLDITASKEKRNILKNLVEEIAKELFIPFTVG
GGLKTVEQMVEIIKCGADKVFINSAAVENPNLIKESSKIIGSSNVVVAIDAKKDVESEKYYVYTHGGSKKTDLDAVEWAR
KCQELGAGELLVTSMNTDGVKKGYDLNLTKQIVDAVEIPVIASGGAGEVKDFIDVFQIGADAALAASIFHYGIYTAKDLK
IQLKKVGINVRL

Sequences:

>Translated_252_residues
MLTKRIVAALDIKEGRVVKGVQFENIRDAGDPVELAKKYEKDGVDEIVFLDITASKEKRNILKNLVEEIAKELFIPFTVG
GGLKTVEQMVEIIKCGADKVFINSAAVENPNLIKESSKIIGSSNVVVAIDAKKDVESEKYYVYTHGGSKKTDLDAVEWAR
KCQELGAGELLVTSMNTDGVKKGYDLNLTKQIVDAVEIPVIASGGAGEVKDFIDVFQIGADAALAASIFHYGIYTAKDLK
IQLKKVGINVRL
>Mature_252_residues
MLTKRIVAALDIKEGRVVKGVQFENIRDAGDPVELAKKYEKDGVDEIVFLDITASKEKRNILKNLVEEIAKELFIPFTVG
GGLKTVEQMVEIIKCGADKVFINSAAVENPNLIKESSKIIGSSNVVVAIDAKKDVESEKYYVYTHGGSKKTDLDAVEWAR
KCQELGAGELLVTSMNTDGVKKGYDLNLTKQIVDAVEIPVIASGGAGEVKDFIDVFQIGADAALAASIFHYGIYTAKDLK
IQLKKVGINVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=42.4124513618677, Blast_Score=210, Evalue=7e-56,
Organism=Escherichia coli, GI87082028, Length=239, Percent_Identity=23.8493723849372, Blast_Score=74, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=34.0764331210191, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_PETMO (A9BJZ9)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568065.1
- ProteinModelPortal:   A9BJZ9
- SMR:   A9BJZ9
- GeneID:   5756586
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1021
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- BioCyc:   PMOB403833:PMOB_1021-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27448; Mature: 27448

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIVAALDIKEGRVVKGVQFENIRDAGDPVELAKKYEKDGVDEIVFLDITASKEKRN
CCCHHHHHEEECCCCCEEECEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCHHHHH
ILKNLVEEIAKELFIPFTVGGGLKTVEQMVEIIKCGADKVFINSAAVENPNLIKESSKII
HHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHC
GSSNVVVAIDAKKDVESEKYYVYTHGGSKKTDLDAVEWARKCQELGAGELLVTSMNTDGV
CCCCEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
KKGYDLNLTKQIVDAVEIPVIASGGAGEVKDFIDVFQIGADAALAASIFHYGIYTAKDLK
CCCCCCCHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCEEEHHHE
IQLKKVGINVRL
EEEEEECEEECC
>Mature Secondary Structure
MLTKRIVAALDIKEGRVVKGVQFENIRDAGDPVELAKKYEKDGVDEIVFLDITASKEKRN
CCCHHHHHEEECCCCCEEECEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCHHHHH
ILKNLVEEIAKELFIPFTVGGGLKTVEQMVEIIKCGADKVFINSAAVENPNLIKESSKII
HHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHC
GSSNVVVAIDAKKDVESEKYYVYTHGGSKKTDLDAVEWARKCQELGAGELLVTSMNTDGV
CCCCEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
KKGYDLNLTKQIVDAVEIPVIASGGAGEVKDFIDVFQIGADAALAASIFHYGIYTAKDLK
CCCCCCCHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCEEEHHHE
IQLKKVGINVRL
EEEEEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA