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The map label for this gene is pvuIIM [H]

Identifier: 160901891

GI number: 160901891

Start: 440664

End: 441680

Strand: Direct

Name: pvuIIM [H]

Synonym: Pmob_0407

Alternate gene names: 160901891

Gene position: 440664-441680 (Clockwise)

Preceding gene: 160901890

Following gene: 160901892

Centisome position: 20.31

GC content: 32.25

Gene sequence:

>1017_bases
GTGCGTAAACTTTTATATAAAACCGATTACGGGAAGTACTATATTGGTAAATGTGAGTCAGAAATTAAAAGATTAAAACT
TAAAGGGAAAGTCCAATTGATTTTAACTTCGCCGCCCTTTCCATTAAATAATAAAAAACAATATGGAAATTTAGTTGGAG
AAGATTATTTAAACTGGATTACAAATCTGGCACCGCTGTTTTCTGAAGCTTTAACAGATAACGGTTCTATTGTAATAGAA
ATGGGAAATGCATGGGAAAAAGATCGACCTGTACAATCACTGTTGCATTTAAAATCTTTAATGAGATTTGTGGAGAATCC
AGAGGCAAACCTACGACTTTGTCAAGAATTTATTTGTTATAATCCGGCAAGATTACCTTCACCGGCACAATGGGTTACCA
TTGAACGCATTCGTGCTATTGATAGTTTTACACATGTATGGTGGATGTCTAAAACAGATTATCCTAAAGCTGATAATAGA
AAGATTTTAAGGCCTTATAGTAAAAGCATGAAAAAACTACTTGAAAAAGGAAAATACAATTCAGGTAAAAGACCTTCTGA
ACACGTGATAAGCGAGAAAAGTTTTTTGAAAGATAATAAAGGAAGTATATCCCATAATGTTCTAGAACTTAAGCAAATTG
ATGAAACAAAAGAATTAAGGCTTCCTTATTCCATGTTAAGTATTTCTAATACAAAATCTAATGATTATTTTACAAAAACA
TGTAAGAAGAGAAATATTATACCTCATCCAGCAAGGATGCCATTAGAATTAGCAAGTTTTTTTATTGAATTCTTAACGGA
TGAAGGAGATCTTGTTTTAGACCCTTTTGGCGGAAGTAATACTACCGGATTTTGCGCTGAGAAACTAAAAAGAAAATGGA
TCAGCATTGAAGCTAATGAGGAATATGGTTATCAATCTATAATAAGATTTGAGGAACCATCATTTAATGCTAATATAAGA
AAGGGGGAATTTTTTAATGGGATTCAATCAGGAAATTTTGAAAATGGCGGTCGTTGA

Upstream 100 bases:

>100_bases
TGCGTGTGGTGGGTTGAGCGATATCAAATGATTTGGAGAAACCAAAAAATACAAGGCTTAGAGTGTATAAATTTGATAAT
TATAGAAAGAGTGATTTCTA

Downstream 100 bases:

>100_bases
TATTTTTGCCGAAGCAAAAAAGCCTGACTTGTTTATTGGGCGTCCTTATTATTTGGATTATGATAAGGCCTATCTTCTTA
TCGCTGATGCATGGAAACAA

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.PvuII; N-4 cytosine-specific methyltransferase PvuII [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MRKLLYKTDYGKYYIGKCESEIKRLKLKGKVQLILTSPPFPLNNKKQYGNLVGEDYLNWITNLAPLFSEALTDNGSIVIE
MGNAWEKDRPVQSLLHLKSLMRFVENPEANLRLCQEFICYNPARLPSPAQWVTIERIRAIDSFTHVWWMSKTDYPKADNR
KILRPYSKSMKKLLEKGKYNSGKRPSEHVISEKSFLKDNKGSISHNVLELKQIDETKELRLPYSMLSISNTKSNDYFTKT
CKKRNIIPHPARMPLELASFFIEFLTDEGDLVLDPFGGSNTTGFCAEKLKRKWISIEANEEYGYQSIIRFEEPSFNANIR
KGEFFNGIQSGNFENGGR

Sequences:

>Translated_338_residues
MRKLLYKTDYGKYYIGKCESEIKRLKLKGKVQLILTSPPFPLNNKKQYGNLVGEDYLNWITNLAPLFSEALTDNGSIVIE
MGNAWEKDRPVQSLLHLKSLMRFVENPEANLRLCQEFICYNPARLPSPAQWVTIERIRAIDSFTHVWWMSKTDYPKADNR
KILRPYSKSMKKLLEKGKYNSGKRPSEHVISEKSFLKDNKGSISHNVLELKQIDETKELRLPYSMLSISNTKSNDYFTKT
CKKRNIIPHPARMPLELASFFIEFLTDEGDLVLDPFGGSNTTGFCAEKLKRKWISIEANEEYGYQSIIRFEEPSFNANIR
KGEFFNGIQSGNFENGGR
>Mature_338_residues
MRKLLYKTDYGKYYIGKCESEIKRLKLKGKVQLILTSPPFPLNNKKQYGNLVGEDYLNWITNLAPLFSEALTDNGSIVIE
MGNAWEKDRPVQSLLHLKSLMRFVENPEANLRLCQEFICYNPARLPSPAQWVTIERIRAIDSFTHVWWMSKTDYPKADNR
KILRPYSKSMKKLLEKGKYNSGKRPSEHVISEKSFLKDNKGSISHNVLELKQIDETKELRLPYSMLSISNTKSNDYFTKT
CKKRNIIPHPARMPLELASFFIEFLTDEGDLVLDPFGGSNTTGFCAEKLKRKWISIEANEEYGYQSIIRFEEPSFNANIR
KGEFFNGIQSGNFENGGR

Specific function: This methylase recognizes the double-stranded sequence CAGCTG, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the PvuII endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 39091; Mature: 39091

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLLYKTDYGKYYIGKCESEIKRLKLKGKVQLILTSPPFPLNNKKQYGNLVGEDYLNWI
CCCCEEECCCCCEEEHHHHHHHHHHEECCEEEEEEECCCCCCCCCHHHCCHHHHHHHHHH
TNLAPLFSEALTDNGSIVIEMGNAWEKDRPVQSLLHLKSLMRFVENPEANLRLCQEFICY
HHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
NPARLPSPAQWVTIERIRAIDSFTHVWWMSKTDYPKADNRKILRPYSKSMKKLLEKGKYN
CCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
SGKRPSEHVISEKSFLKDNKGSISHNVLELKQIDETKELRLPYSMLSISNTKSNDYFTKT
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHCCCHHHHEECCCCCCCHHHHH
CKKRNIIPHPARMPLELASFFIEFLTDEGDLVLDPFGGSNTTGFCAEKLKRKWISIEANE
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHEEEECCC
EYGYQSIIRFEEPSFNANIRKGEFFNGIQSGNFENGGR
CCCCHHHEEECCCCCCCCCCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRKLLYKTDYGKYYIGKCESEIKRLKLKGKVQLILTSPPFPLNNKKQYGNLVGEDYLNWI
CCCCEEECCCCCEEEHHHHHHHHHHEECCEEEEEEECCCCCCCCCHHHCCHHHHHHHHHH
TNLAPLFSEALTDNGSIVIEMGNAWEKDRPVQSLLHLKSLMRFVENPEANLRLCQEFICY
HHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
NPARLPSPAQWVTIERIRAIDSFTHVWWMSKTDYPKADNRKILRPYSKSMKKLLEKGKYN
CCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
SGKRPSEHVISEKSFLKDNKGSISHNVLELKQIDETKELRLPYSMLSISNTKSNDYFTKT
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHCCCHHHHEECCCCCCCHHHHH
CKKRNIIPHPARMPLELASFFIEFLTDEGDLVLDPFGGSNTTGFCAEKLKRKWISIEANE
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHEEEECCC
EYGYQSIIRFEEPSFNANIRKGEFFNGIQSGNFENGGR
CCCCHHHEEECCCCCCCCCCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2662138; 7607491; 9207015 [H]