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The map label for this gene is ybjF [C]

Identifier: 160901890

GI number: 160901890

Start: 438956

End: 440119

Strand: Direct

Name: ybjF [C]

Synonym: Pmob_0406

Alternate gene names: 160901890

Gene position: 438956-440119 (Clockwise)

Preceding gene: 160901889

Following gene: 160901891

Centisome position: 20.23

GC content: 31.36

Gene sequence:

>1164_bases
ATGCAGATATGTTCTGTCTTCAAGAAATGTGGTGGATGTAGTAAATTGCATATAAATTACGAAGAACAACTAAAAATAAA
AGAGAAAAACGTTCTTTCACTCTTTGATCAATATCAAATAAAACCTAATAATTATCATGGTATTACTCCTTCTGTAGATA
TATATGAATACAGAAACAAAATGGAATACTCTTTTGGCAACGAATACAAAGATGGCCCTCTTGTTTTGGGGTTAAGAGGA
AAAGGTAAGAAGTTCGACGTCTATTACACAAAAGACTGTAAACTAGTGGATTATGACTTCAACAATATCGTTATAAAAAC
AAGAGAATTTTTCTTAAAAAAGAACATACCTTTTAGAAATTACAAGAACCACTCAGGTTTTCTTAGAAATTTGGGAATCA
GAAAAGGATTAAAAACTGGGGAAATACTTTTAAACTTGGTAACTTCCTTAGATAATACTTACTATCAAGAAGTAGAAACA
TGGAAAGAAGAAATGTTGAATACTAACTTAAAAGGAAATATAGTTTCCATAATTCAAAGCAAAACTGAATCTAAAGCCAA
CGTTGTAAAAGCGGATGAAATGGAAATATTATACGGCAGAGACTATTTTTACGAAAAAATTTTAGATTTAACTTTCAAAA
TAGGTCCTTTCTCTTTCTTTCAAACAAACACAAAAGGAACAGAGGTTTTATACCAAACTGCGTTATCTTATGCAGAAAAT
TCAGATGTTGTATATGACTTTTATTCTGGAACGGGCACAATATCCCTATTACTCGCAAATAAAGCGAAAAAAGTCTATGG
AATAGAAATTATAAAAGAAGCAGTAGAGGCAGCTAAGCAAAATGCCATATTAAATAATATCAACAACGTAGAATTCGTAA
ATAAAGATGTAAAAGATTTTGTTAAGCAACAAAGCAGCACCGAAAAACCTGATTATATAGTGGTGGACCCACCAAGGGCT
GGGTTACATCCAAATCTTATAAAATTCATAAAAACCCAGCAATTTAATAAAATTATTTATATCTCATGCAACCCTAAAAC
CCTAATACCCAATTTGAAAGAATTATCCGATCTCTACACAATCTCAGATTTTCATCTGGTAGATATGTTTCCTCACACTG
ACCACGTTGAGTCAGTAGTATTGATGACGAGGATAGATAAATAG

Upstream 100 bases:

>100_bases
AAAATAAGGTTAAAGTAAAAAACTAACTATTTCCCCCGAAAAGTGTTAAAATTGAACTGTTCGGGGGTATTTTTTTGATT
ATCGAAAAAGGAGCTATAAA

Downstream 100 bases:

>100_bases
TAGAGTTGAAAAAGGGTTTGAATACAGGGTTTTCCTGTGATTTGCCATCAACTGATAATGCGCTTTTTGGCGGCGAAGTT
AACTGGAAGAGACATATTTT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MQICSVFKKCGGCSKLHINYEEQLKIKEKNVLSLFDQYQIKPNNYHGITPSVDIYEYRNKMEYSFGNEYKDGPLVLGLRG
KGKKFDVYYTKDCKLVDYDFNNIVIKTREFFLKKNIPFRNYKNHSGFLRNLGIRKGLKTGEILLNLVTSLDNTYYQEVET
WKEEMLNTNLKGNIVSIIQSKTESKANVVKADEMEILYGRDYFYEKILDLTFKIGPFSFFQTNTKGTEVLYQTALSYAEN
SDVVYDFYSGTGTISLLLANKAKKVYGIEIIKEAVEAAKQNAILNNINNVEFVNKDVKDFVKQQSSTEKPDYIVVDPPRA
GLHPNLIKFIKTQQFNKIIYISCNPKTLIPNLKELSDLYTISDFHLVDMFPHTDHVESVVLMTRIDK

Sequences:

>Translated_387_residues
MQICSVFKKCGGCSKLHINYEEQLKIKEKNVLSLFDQYQIKPNNYHGITPSVDIYEYRNKMEYSFGNEYKDGPLVLGLRG
KGKKFDVYYTKDCKLVDYDFNNIVIKTREFFLKKNIPFRNYKNHSGFLRNLGIRKGLKTGEILLNLVTSLDNTYYQEVET
WKEEMLNTNLKGNIVSIIQSKTESKANVVKADEMEILYGRDYFYEKILDLTFKIGPFSFFQTNTKGTEVLYQTALSYAEN
SDVVYDFYSGTGTISLLLANKAKKVYGIEIIKEAVEAAKQNAILNNINNVEFVNKDVKDFVKQQSSTEKPDYIVVDPPRA
GLHPNLIKFIKTQQFNKIIYISCNPKTLIPNLKELSDLYTISDFHLVDMFPHTDHVESVVLMTRIDK
>Mature_387_residues
MQICSVFKKCGGCSKLHINYEEQLKIKEKNVLSLFDQYQIKPNNYHGITPSVDIYEYRNKMEYSFGNEYKDGPLVLGLRG
KGKKFDVYYTKDCKLVDYDFNNIVIKTREFFLKKNIPFRNYKNHSGFLRNLGIRKGLKTGEILLNLVTSLDNTYYQEVET
WKEEMLNTNLKGNIVSIIQSKTESKANVVKADEMEILYGRDYFYEKILDLTFKIGPFSFFQTNTKGTEVLYQTALSYAEN
SDVVYDFYSGTGTISLLLANKAKKVYGIEIIKEAVEAAKQNAILNNINNVEFVNKDVKDFVKQQSSTEKPDYIVVDPPRA
GLHPNLIKFIKTQQFNKIIYISCNPKTLIPNLKELSDLYTISDFHLVDMFPHTDHVESVVLMTRIDK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=384, Percent_Identity=29.1666666666667, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI51173878, Length=384, Percent_Identity=29.1666666666667, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI269784680, Length=416, Percent_Identity=27.8846153846154, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI269784676, Length=416, Percent_Identity=27.8846153846154, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI20149688, Length=416, Percent_Identity=27.8846153846154, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI269784678, Length=209, Percent_Identity=33.9712918660287, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1787083, Length=388, Percent_Identity=25.7731958762887, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789148, Length=393, Percent_Identity=26.4631043256997, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790403, Length=250, Percent_Identity=30, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71997438, Length=175, Percent_Identity=31.4285714285714, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24666579, Length=426, Percent_Identity=31.4553990610329, Blast_Score=165, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 44754; Mature: 44754

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQICSVFKKCGGCSKLHINYEEQLKIKEKNVLSLFDQYQIKPNNYHGITPSVDIYEYRNK
CCHHHHHHHCCCCEEEEECHHHHEEECHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHCH
MEYSFGNEYKDGPLVLGLRGKGKKFDVYYTKDCKLVDYDFNNIVIKTREFFLKKNIPFRN
HCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCEEEEECCCCEEEEEHHHHHHCCCCCCC
YKNHSGFLRNLGIRKGLKTGEILLNLVTSLDNTYYQEVETWKEEMLNTNLKGNIVSIIQS
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
KTESKANVVKADEMEILYGRDYFYEKILDLTFKIGPFSFFQTNTKGTEVLYQTALSYAEN
HCCCCCCEEECCCEEEEECCHHHHHHHHHHEEEECCEEEEECCCCHHHHHHHHHHHHCCC
SDVVYDFYSGTGTISLLLANKAKKVYGIEIIKEAVEAAKQNAILNNINNVEFVNKDVKDF
CCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VKQQSSTEKPDYIVVDPPRAGLHPNLIKFIKTQQFNKIIYISCNPKTLIPNLKELSDLYT
HHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHE
ISDFHLVDMFPHTDHVESVVLMTRIDK
ECCEEEEEECCCCHHHHHEEEEEECCC
>Mature Secondary Structure
MQICSVFKKCGGCSKLHINYEEQLKIKEKNVLSLFDQYQIKPNNYHGITPSVDIYEYRNK
CCHHHHHHHCCCCEEEEECHHHHEEECHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHCH
MEYSFGNEYKDGPLVLGLRGKGKKFDVYYTKDCKLVDYDFNNIVIKTREFFLKKNIPFRN
HCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCEEEEECCCCEEEEEHHHHHHCCCCCCC
YKNHSGFLRNLGIRKGLKTGEILLNLVTSLDNTYYQEVETWKEEMLNTNLKGNIVSIIQS
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
KTESKANVVKADEMEILYGRDYFYEKILDLTFKIGPFSFFQTNTKGTEVLYQTALSYAEN
HCCCCCCEEECCCEEEEECCHHHHHHHHHHEEEECCEEEEECCCCHHHHHHHHHHHHCCC
SDVVYDFYSGTGTISLLLANKAKKVYGIEIIKEAVEAAKQNAILNNINNVEFVNKDVKDF
CCEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VKQQSSTEKPDYIVVDPPRAGLHPNLIKFIKTQQFNKIIYISCNPKTLIPNLKELSDLYT
HHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHE
ISDFHLVDMFPHTDHVESVVLMTRIDK
ECCEEEEEECCCCHHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]