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The map label for this gene is metF [H]

Identifier: 160901802

GI number: 160901802

Start: 342709

End: 343656

Strand: Direct

Name: metF [H]

Synonym: Pmob_0318

Alternate gene names: 160901802

Gene position: 342709-343656 (Clockwise)

Preceding gene: 160901801

Following gene: 160901803

Centisome position: 15.8

GC content: 33.65

Gene sequence:

>948_bases
ATGAAAATAGTAGATTTGATCAAACAATCAAACAAACCTGTTCTTTCCTTTGAAATTATCCCCCCTAATGTTGGAGAAAG
TATTGATTCAATATTTAATGTTGTGGATAATTTAATCGAATTTTCTCCTAAATATATCAATGTCACTAAGCACGCCAACG
AAATAGACTATGTTGAAGAAAATGGGAAGATTATGAAAATCATCCAAAAGAAGAGACCTGGGACCGTCGGAGTAAGTGCT
TCAATAAAACATAGATATAATATCGAAGTAGTCCCTCACTTAATATGTACGGGCTTTAACAAATATCAACTGGAAGACAT
ACTAATAGATCTAAATTATCTCAGGATAGAAAACATATTCGTCGTAAGAGGAGATAAGAAAAGGTATACATGGAAAGAAA
CCGATGAATACGAACATGCAAGTCAATTAGTTGAGCAAATAGCAAATATGAACAGAGGAATATACACGTTCCCAACAAAA
GAACATAATTCTACAAATTTCTGCGTGGGCGTTGCAGGATACCCAGAAAAACATTTTGAATCCCCAAACTTTGAAAAAGA
TTTGGACTATTTGAAATTAAAGGTCGATATGGGGGCAACATTCATAATAACTCAGATGTTTTTTGACATTGAGTATTACA
AAAACTTCGTTAATAAAGTTCGAGATCTCGGCATAGAAGTGCCAATAATCCCAGGCATCAAACCGTTGGGAAGCAAAAAA
ACACTATACAACATTCCTAAAACTTTTCATGTTAATATACCTAAATCCATTGTGGAAGAGTTTGAAAACGCAAAATCATC
TCAAGATGAATATATTATAGGAGTAAGGCACGCAATAAAACTAATTGAGCAACTTTTAGAACTGGGAGTTCCAGGAGTAC
ATCTTTTTACGATGGGGAAAGGAAAGATAGTAAAAGACGTTTTAAGTGCATTTAAGGGAATTTTTTAG

Upstream 100 bases:

>100_bases
CCCTTTTTGTTATGCCGTCTATTAGCTGTTGACTATTTTTTGCCAATATGTTAAAATTGAACAAATGTTTTATTATATAT
AATGTCCGGGGTGAAATACA

Downstream 100 bases:

>100_bases
AATTTCGATGGGTGGGGGAGCAAAGGAGAAATAAAAATGGCACGCCCGGAGGGAATCGAACCCCCAACCTGCGGTTTTGG
AGACCGCCGCTCTGCCAGTT

Product: methylenetetrahydrofolate reductase (NAD(P)H)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKIVDLIKQSNKPVLSFEIIPPNVGESIDSIFNVVDNLIEFSPKYINVTKHANEIDYVEENGKIMKIIQKKRPGTVGVSA
SIKHRYNIEVVPHLICTGFNKYQLEDILIDLNYLRIENIFVVRGDKKRYTWKETDEYEHASQLVEQIANMNRGIYTFPTK
EHNSTNFCVGVAGYPEKHFESPNFEKDLDYLKLKVDMGATFIITQMFFDIEYYKNFVNKVRDLGIEVPIIPGIKPLGSKK
TLYNIPKTFHVNIPKSIVEEFENAKSSQDEYIIGVRHAIKLIEQLLELGVPGVHLFTMGKGKIVKDVLSAFKGIF

Sequences:

>Translated_315_residues
MKIVDLIKQSNKPVLSFEIIPPNVGESIDSIFNVVDNLIEFSPKYINVTKHANEIDYVEENGKIMKIIQKKRPGTVGVSA
SIKHRYNIEVVPHLICTGFNKYQLEDILIDLNYLRIENIFVVRGDKKRYTWKETDEYEHASQLVEQIANMNRGIYTFPTK
EHNSTNFCVGVAGYPEKHFESPNFEKDLDYLKLKVDMGATFIITQMFFDIEYYKNFVNKVRDLGIEVPIIPGIKPLGSKK
TLYNIPKTFHVNIPKSIVEEFENAKSSQDEYIIGVRHAIKLIEQLLELGVPGVHLFTMGKGKIVKDVLSAFKGIF
>Mature_315_residues
MKIVDLIKQSNKPVLSFEIIPPNVGESIDSIFNVVDNLIEFSPKYINVTKHANEIDYVEENGKIMKIIQKKRPGTVGVSA
SIKHRYNIEVVPHLICTGFNKYQLEDILIDLNYLRIENIFVVRGDKKRYTWKETDEYEHASQLVEQIANMNRGIYTFPTK
EHNSTNFCVGVAGYPEKHFESPNFEKDLDYLKLKVDMGATFIITQMFFDIEYYKNFVNKVRDLGIEVPIIPGIKPLGSKK
TLYNIPKTFHVNIPKSIVEEFENAKSSQDEYIIGVRHAIKLIEQLLELGVPGVHLFTMGKGKIVKDVLSAFKGIF

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=309, Percent_Identity=29.7734627831715, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI1790377, Length=290, Percent_Identity=29.3103448275862, Blast_Score=122, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI25147436, Length=299, Percent_Identity=28.0936454849498, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25147441, Length=299, Percent_Identity=28.0936454849498, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321313, Length=311, Percent_Identity=28.6173633440514, Blast_Score=122, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6325234, Length=326, Percent_Identity=27.9141104294479, Blast_Score=116, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24662658, Length=243, Percent_Identity=27.5720164609054, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 36133; Mature: 36133

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVDLIKQSNKPVLSFEIIPPNVGESIDSIFNVVDNLIEFSPKYINVTKHANEIDYVEE
CCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEECC
NGKIMKIIQKKRPGTVGVSASIKHRYNIEVVPHLICTGFNKYQLEDILIDLNYLRIENIF
CCCEEEHHHHCCCCCEEEEEEECEEECEEEHHHHHHCCCCCEEHHHHEEEEEEEEEEEEE
VVRGDKKRYTWKETDEYEHASQLVEQIANMNRGIYTFPTKEHNSTNFCVGVAGYPEKHFE
EEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCHHHCC
SPNFEKDLDYLKLKVDMGATFIITQMFFDIEYYKNFVNKVRDLGIEVPIIPGIKPLGSKK
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
TLYNIPKTFHVNIPKSIVEEFENAKSSQDEYIIGVRHAIKLIEQLLELGVPGVHLFTMGK
EEEECCCEEEECCCHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEECCC
GKIVKDVLSAFKGIF
CHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIVDLIKQSNKPVLSFEIIPPNVGESIDSIFNVVDNLIEFSPKYINVTKHANEIDYVEE
CCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEECC
NGKIMKIIQKKRPGTVGVSASIKHRYNIEVVPHLICTGFNKYQLEDILIDLNYLRIENIF
CCCEEEHHHHCCCCCEEEEEEECEEECEEEHHHHHHCCCCCEEHHHHEEEEEEEEEEEEE
VVRGDKKRYTWKETDEYEHASQLVEQIANMNRGIYTFPTKEHNSTNFCVGVAGYPEKHFE
EEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCHHHCC
SPNFEKDLDYLKLKVDMGATFIITQMFFDIEYYKNFVNKVRDLGIEVPIIPGIKPLGSKK
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
TLYNIPKTFHVNIPKSIVEEFENAKSSQDEYIIGVRHAIKLIEQLLELGVPGVHLFTMGK
EEEECCCEEEECCCHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEECCC
GKIVKDVLSAFKGIF
CHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]