Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 160901753

GI number: 160901753

Start: 283918

End: 284991

Strand: Direct

Name: braE [H]

Synonym: Pmob_0267

Alternate gene names: 160901753

Gene position: 283918-284991 (Clockwise)

Preceding gene: 160901752

Following gene: 160901754

Centisome position: 13.09

GC content: 35.29

Gene sequence:

>1074_bases
ATGGAAAAAACGCTATCTTTTAAGACTAAATTCTTTTTGACAATTCTTTTTATATTGGCAATCTTTCTATTACTGTTGAC
AGCTAATAACAAAGCTAGTGATTATTTAATTTTGATATTGAATTTAATGGCAATTAATATAATATTCGCCGTAAGTTTAA
CCTTTATTAACGGTATTACAGGTATCTTTTCGTTGGGGCATGTTGGATTCATCGCAATTGGGGCATATGTATCGTCCATT
TTAACACTTTCTCCTGCTCAAAAAGAAATAAGTTTTCTGATAAAACCTTTAATATACCCCTTAAATGTTATCCAAATCCC
TTTCCTACCAGCAATTATAATAGCTGGACTGGTAGCTGCTGCGTTTGGTTACTTAGTTGCTGCCCCATCTCTTCGACTAA
TTGGTGATTATTTAGCCATCGCAACCCTTGGTTTAGGTGAGGTGGTTAGAATCGTTGCAAATAATACCTGGTCTATCACA
AATGGGGCTTTGGGTTTAAAAAGCATCCCACAATATTCTAACCTATGGTGGACTTGGGGATTTGCTTTAATTACCGTAGT
CTTCATTTCCAGCTTAATAAAAAGTAGTTATGGAAGAGCTTTGAAGGCAATAAGAGAAGATCCAATTGCTGCTAAATCTA
TGGGAATTAATGTTTTTTCTCATCAAGTAGTCACTTTTGTAATTGGTTCTTTTTTTGCAGGAGTTGGTGGAGCATTATGG
GCTCACTTAATCACTACTATAGATCCAAAATCTTTCATGTTTCAAAAAACCTTTGAAATATTGATAATGGTGGTCATCGG
TGGACTTGGGAGTATTAGCGGAGCCATAATCGGAGCTTCACTTTATACAGTAGGATTAGAGTTCCTTAGAGTTCTAGAAG
AACCCATATCGATTGGTCCTATCTACATACCAGGTATCCCGGGAATGAGAATGGTGGTTTTATCTCTCATTCTCATAATT
ACAATGCTATTTTGGAGAAGAGGCATAATGGGAAGAAATGAAATAACATGGGATGGCATCTATAAACTGATAATGAAAGT
AAGAAATAAAAATAGATTGAGAGATGATGAATGA

Upstream 100 bases:

>100_bases
CAGGATATAGAGACGCTATTGCTTATATTATTTTGATAGTTATTCTTTTAGTAAAACCAAACGGGCTTCTCGGTGTGGAA
ATAGGGGAGAAGGTGTGAAT

Downstream 100 bases:

>100_bases
ATTAGAAAATTTTCAAGGAGGCTTCCAATGACTAACCAATACGATAACGAATATATCTTAGAATTTGAGAATGTAACCAA
AAGATTTGGAGGTTTAATGG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MEKTLSFKTKFFLTILFILAIFLLLLTANNKASDYLILILNLMAINIIFAVSLTFINGITGIFSLGHVGFIAIGAYVSSI
LTLSPAQKEISFLIKPLIYPLNVIQIPFLPAIIIAGLVAAAFGYLVAAPSLRLIGDYLAIATLGLGEVVRIVANNTWSIT
NGALGLKSIPQYSNLWWTWGFALITVVFISSLIKSSYGRALKAIREDPIAAKSMGINVFSHQVVTFVIGSFFAGVGGALW
AHLITTIDPKSFMFQKTFEILIMVVIGGLGSISGAIIGASLYTVGLEFLRVLEEPISIGPIYIPGIPGMRMVVLSLILII
TMLFWRRGIMGRNEITWDGIYKLIMKVRNKNRLRDDE

Sequences:

>Translated_357_residues
MEKTLSFKTKFFLTILFILAIFLLLLTANNKASDYLILILNLMAINIIFAVSLTFINGITGIFSLGHVGFIAIGAYVSSI
LTLSPAQKEISFLIKPLIYPLNVIQIPFLPAIIIAGLVAAAFGYLVAAPSLRLIGDYLAIATLGLGEVVRIVANNTWSIT
NGALGLKSIPQYSNLWWTWGFALITVVFISSLIKSSYGRALKAIREDPIAAKSMGINVFSHQVVTFVIGSFFAGVGGALW
AHLITTIDPKSFMFQKTFEILIMVVIGGLGSISGAIIGASLYTVGLEFLRVLEEPISIGPIYIPGIPGMRMVVLSLILII
TMLFWRRGIMGRNEITWDGIYKLIMKVRNKNRLRDDE
>Mature_357_residues
MEKTLSFKTKFFLTILFILAIFLLLLTANNKASDYLILILNLMAINIIFAVSLTFINGITGIFSLGHVGFIAIGAYVSSI
LTLSPAQKEISFLIKPLIYPLNVIQIPFLPAIIIAGLVAAAFGYLVAAPSLRLIGDYLAIATLGLGEVVRIVANNTWSIT
NGALGLKSIPQYSNLWWTWGFALITVVFISSLIKSSYGRALKAIREDPIAAKSMGINVFSHQVVTFVIGSFFAGVGGALW
AHLITTIDPKSFMFQKTFEILIMVVIGGLGSISGAIIGASLYTVGLEFLRVLEEPISIGPIYIPGIPGMRMVVLSLILII
TMLFWRRGIMGRNEITWDGIYKLIMKVRNKNRLRDDE

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=290, Percent_Identity=32.7586206896552, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 39094; Mature: 39094

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKTLSFKTKFFLTILFILAIFLLLLTANNKASDYLILILNLMAINIIFAVSLTFINGIT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIFSLGHVGFIAIGAYVSSILTLSPAQKEISFLIKPLIYPLNVIQIPFLPAIIIAGLVAA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGYLVAAPSLRLIGDYLAIATLGLGEVVRIVANNTWSITNGALGLKSIPQYSNLWWTWG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHCCCCHHCHHHHHH
FALITVVFISSLIKSSYGRALKAIREDPIAAKSMGINVFSHQVVTFVIGSFFAGVGGALW
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AHLITTIDPKSFMFQKTFEILIMVVIGGLGSISGAIIGASLYTVGLEFLRVLEEPISIGP
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IYIPGIPGMRMVVLSLILIITMLFWRRGIMGRNEITWDGIYKLIMKVRNKNRLRDDE
EEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEKTLSFKTKFFLTILFILAIFLLLLTANNKASDYLILILNLMAINIIFAVSLTFINGIT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIFSLGHVGFIAIGAYVSSILTLSPAQKEISFLIKPLIYPLNVIQIPFLPAIIIAGLVAA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGYLVAAPSLRLIGDYLAIATLGLGEVVRIVANNTWSITNGALGLKSIPQYSNLWWTWG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHCCCCHHCHHHHHH
FALITVVFISSLIKSSYGRALKAIREDPIAAKSMGINVFSHQVVTFVIGSFFAGVGGALW
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
AHLITTIDPKSFMFQKTFEILIMVVIGGLGSISGAIIGASLYTVGLEFLRVLEEPISIGP
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IYIPGIPGMRMVVLSLILIITMLFWRRGIMGRNEITWDGIYKLIMKVRNKNRLRDDE
EEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]