Click here to switch to the map view.

The map label for this gene is nplT [H]

Identifier: 160901738

GI number: 160901738

Start: 269617

End: 270927

Strand: Direct

Name: nplT [H]

Synonym: Pmob_0252

Alternate gene names: 160901738

Gene position: 269617-270927 (Clockwise)

Preceding gene: 160901729

Following gene: 160901743

Centisome position: 12.43

GC content: 29.82

Gene sequence:

>1311_bases
ATGTTTTTAGGATACCACATTCTAATAAAATTTTATAAAAATGGAGAATTAACAAATGATTTTCCAAAGGAGAGTTATCG
CTATTACGAAAAAAATGCAATAGATGGAGATCTTCAAGGTGTCATTAAAGAATTAGACTATTTAAAAGAGCTTGGGATAG
ATTTGATATATCTTGGGCCTATTTTTAAAAGTAAAACCACACACGGATACGATATTACCAATTATTTTTCTATCTCAGAA
AACATAAGTTCTAATTCCAAAGAAGAAGCTAAAGCTATCTTTACAAAGCTTATTGAAGATGCTCATAAAAGAGGAATAAA
AGTTATTATCGATCTAGTACTTAACCATGCCTCAAAGGAGTTTGATTTTAATTCCATCCCTAAAGATTTGAACGTTAAAA
CCGAATCTCCCCGTTCACCTCAAGAAGAAAGATGGCAAAGATCCTTTTTGTTCTGGAATCTAGATGACGAAGATACAAGA
GAATTTTTAATAAGAGTTGGAGAGTATTGGCTAAAAAACTTTGAATTAGACGGATTTAGATTGGATCATGCCTTAGGCTT
GCCATTAAACTTTTTAGAGGAATTTTCTATAAGGATGAAAAAAATAAAAAAAGATGCGATAATTTTAGGTGAAGTATGGG
AAGATGAAGGAGATAAGTTAAAAAATTTTAATCTTCTTAAAAAGTTCAAGGGAGATGATGCTCAACGTTTTACCAGTATG
TTTGATTTTTCTACATACGATACTATTAAAGAGGTTTTGGGAAAGAAAAATGGATCGTTAATCGAGCTATACGATCAAAT
AGTATTATCAAACAAATTAAACGAAAGAGAATTCCAGTTAACTTATTTTATAGAAAATCATGATCTTCCAAGGTTCATAG
ATATATGCCAAGAAATAGAGATATTTTACATTGCGGTTGGTTTATTAATGGCTCTTACAGGTAATGTGATGCTGGAATAT
GGCAATGAGATAGCTTTAAAAGGAGACCAAACAATTAATAATTTTCACGAAAGTGGAAGAGTTGCGATGAAATTCAAAGA
AGATTGGTCAAATTTAGAAAAAGAAACCTTCAATTACTGTAAAAATCTCATTAACTTAAGGAAAGTTCACACGTCTCTTT
CATCAGGGAACTATGAATTTGTTAATTCAGAAGAAAATTTACTGATTTTTAACAAGAAAGAGGGATATGATGAGATAAAG
GTAGTCATTTATATCGGAGAACAAGAGTATCATTTGGATAAAGAATATTTAGATCTAATTAGAAATACAAAAGTTTTGAA
ATTAAAAAAAGGCATTTATTATCTTCAATAA

Upstream 100 bases:

>100_bases
AATTTTAAAAGTAAAAAAAGTGAATATCTTTAGGAAACGTTTTCATTTCTTTAAAAAAAGAGTGGTAAAATAACGAGAAG
TAATCTATGGGAGGTATTTT

Downstream 100 bases:

>100_bases
ATTTAAAATATATTTTTCAGCTCTATGAGCTTGGATATACTGAGAACATCAGTCTCTTGGGTTTCAAGAGAATCTTCATG
ATTTTTAAACCAAATGGCGC

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MFLGYHILIKFYKNGELTNDFPKESYRYYEKNAIDGDLQGVIKELDYLKELGIDLIYLGPIFKSKTTHGYDITNYFSISE
NISSNSKEEAKAIFTKLIEDAHKRGIKVIIDLVLNHASKEFDFNSIPKDLNVKTESPRSPQEERWQRSFLFWNLDDEDTR
EFLIRVGEYWLKNFELDGFRLDHALGLPLNFLEEFSIRMKKIKKDAIILGEVWEDEGDKLKNFNLLKKFKGDDAQRFTSM
FDFSTYDTIKEVLGKKNGSLIELYDQIVLSNKLNEREFQLTYFIENHDLPRFIDICQEIEIFYIAVGLLMALTGNVMLEY
GNEIALKGDQTINNFHESGRVAMKFKEDWSNLEKETFNYCKNLINLRKVHTSLSSGNYEFVNSEENLLIFNKKEGYDEIK
VVIYIGEQEYHLDKEYLDLIRNTKVLKLKKGIYYLQ

Sequences:

>Translated_436_residues
MFLGYHILIKFYKNGELTNDFPKESYRYYEKNAIDGDLQGVIKELDYLKELGIDLIYLGPIFKSKTTHGYDITNYFSISE
NISSNSKEEAKAIFTKLIEDAHKRGIKVIIDLVLNHASKEFDFNSIPKDLNVKTESPRSPQEERWQRSFLFWNLDDEDTR
EFLIRVGEYWLKNFELDGFRLDHALGLPLNFLEEFSIRMKKIKKDAIILGEVWEDEGDKLKNFNLLKKFKGDDAQRFTSM
FDFSTYDTIKEVLGKKNGSLIELYDQIVLSNKLNEREFQLTYFIENHDLPRFIDICQEIEIFYIAVGLLMALTGNVMLEY
GNEIALKGDQTINNFHESGRVAMKFKEDWSNLEKETFNYCKNLINLRKVHTSLSSGNYEFVNSEENLLIFNKKEGYDEIK
VVIYIGEQEYHLDKEYLDLIRNTKVLKLKKGIYYLQ
>Mature_436_residues
MFLGYHILIKFYKNGELTNDFPKESYRYYEKNAIDGDLQGVIKELDYLKELGIDLIYLGPIFKSKTTHGYDITNYFSISE
NISSNSKEEAKAIFTKLIEDAHKRGIKVIIDLVLNHASKEFDFNSIPKDLNVKTESPRSPQEERWQRSFLFWNLDDEDTR
EFLIRVGEYWLKNFELDGFRLDHALGLPLNFLEEFSIRMKKIKKDAIILGEVWEDEGDKLKNFNLLKKFKGDDAQRFTSM
FDFSTYDTIKEVLGKKNGSLIELYDQIVLSNKLNEREFQLTYFIENHDLPRFIDICQEIEIFYIAVGLLMALTGNVMLEY
GNEIALKGDQTINNFHESGRVAMKFKEDWSNLEKETFNYCKNLINLRKVHTSLSSGNYEFVNSEENLLIFNKKEGYDEIK
VVIYIGEQEYHLDKEYLDLIRNTKVLKLKKGIYYLQ

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1790687, Length=114, Percent_Identity=35.9649122807018, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322245, Length=375, Percent_Identity=27.7333333333333, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321726, Length=371, Percent_Identity=27.2237196765499, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322241, Length=331, Percent_Identity=27.190332326284, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322021, Length=331, Percent_Identity=27.190332326284, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324416, Length=331, Percent_Identity=27.190332326284, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6319776, Length=89, Percent_Identity=38.2022471910112, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321731, Length=89, Percent_Identity=38.2022471910112, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221330053, Length=101, Percent_Identity=42.5742574257426, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24586587, Length=113, Percent_Identity=36.283185840708, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45549022, Length=119, Percent_Identity=33.6134453781513, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 51354; Mature: 51354

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLGYHILIKFYKNGELTNDFPKESYRYYEKNAIDGDLQGVIKELDYLKELGIDLIYLGP
CEEEEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECC
IFKSKTTHGYDITNYFSISENISSNSKEEAKAIFTKLIEDAHKRGIKVIIDLVLNHASKE
HHCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
FDFNSIPKDLNVKTESPRSPQEERWQRSFLFWNLDDEDTREFLIRVGEYWLKNFELDGFR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEE
LDHALGLPLNFLEEFSIRMKKIKKDAIILGEVWEDEGDKLKNFNLLKKFKGDDAQRFTSM
ECHHCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHCCCHHHHHHHH
FDFSTYDTIKEVLGKKNGSLIELYDQIVLSNKLNEREFQLTYFIENHDLPRFIDICQEIE
HCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHH
IFYIAVGLLMALTGNVMLEYGNEIALKGDQTINNFHESGRVAMKFKEDWSNLEKETFNYC
HHHHHHHHHHHHHCCEEEEECCEEEECCCHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHH
KNLINLRKVHTSLSSGNYEFVNSEENLLIFNKKEGYDEIKVVIYIGEQEYHLDKEYLDLI
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCEEEEEEEECCCHHCCCHHHHHHH
RNTKVLKLKKGIYYLQ
CCCCEEEEECCCEECC
>Mature Secondary Structure
MFLGYHILIKFYKNGELTNDFPKESYRYYEKNAIDGDLQGVIKELDYLKELGIDLIYLGP
CEEEEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEECC
IFKSKTTHGYDITNYFSISENISSNSKEEAKAIFTKLIEDAHKRGIKVIIDLVLNHASKE
HHCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCC
FDFNSIPKDLNVKTESPRSPQEERWQRSFLFWNLDDEDTREFLIRVGEYWLKNFELDGFR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCCEE
LDHALGLPLNFLEEFSIRMKKIKKDAIILGEVWEDEGDKLKNFNLLKKFKGDDAQRFTSM
ECHHCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHCCCHHHHHHHH
FDFSTYDTIKEVLGKKNGSLIELYDQIVLSNKLNEREFQLTYFIENHDLPRFIDICQEIE
HCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHHHHHHH
IFYIAVGLLMALTGNVMLEYGNEIALKGDQTINNFHESGRVAMKFKEDWSNLEKETFNYC
HHHHHHHHHHHHHCCEEEEECCEEEECCCHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHH
KNLINLRKVHTSLSSGNYEFVNSEENLLIFNKKEGYDEIKVVIYIGEQEYHLDKEYLDLI
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCEEEEEEEECCCHHCCCHHHHHHH
RNTKVLKLKKGIYYLQ
CCCCEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]