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The map label for this gene is ispE

Identifier: 160901649

GI number: 160901649

Start: 161304

End: 162077

Strand: Direct

Name: ispE

Synonym: Pmob_0160

Alternate gene names: 160901649

Gene position: 161304-162077 (Clockwise)

Preceding gene: 160901648

Following gene: 160901653

Centisome position: 7.43

GC content: 30.36

Gene sequence:

>774_bases
ATGCATTTAAAAGCCTTTGGGAAAGTTAACCTTTACTTAGATGTAATTAGTAGGAGAAAAGATAATTACCATAATATTTT
AACCCTTTTCCAATCTATTAACGAACACGACGATATATTTATTGAATTTTCTGAAAGTGAAATATTCGAATCAGAACCTC
CTTTAAATATTTCATGGGATAATAATATAATAAAAAAGTCCATAGAGACCTTTAAAAAAGAAACGGGTTTTGACAATTTT
AACTTAAAAATTAAGCTTATTAAGAATTTACCTATGGGTGGAGGACTAGGGGGTGGAAGTGCCGACGCTGCTGCTGTCTT
AAACTTCCTTTCAAAGAATTTTAAAATATCAGAAAAAGACTTATTCGAAATAAGTTGCAAAATCGGGAGTGATGTCCCAT
TTTTGCTAAAAGGTGGAACCGCTATAGGTAAAGGGAAAGGAGAAATTTTGGAATTTTTGGAACCTTTAAAATTAAATATT
CAAATATACCCCATGAACTATAATATTGATACTAAAAAAATGTACGAAAAACTTGATCAAAATTGGAAAAGTATAAACCA
TTTCGGTGATCCATACAATTTGTACGAAGCTTTAAAAAATAACGACATTATAACTGCTAAACAAAATGCGTTTAACGTAT
TCGAACAAGTGGCATTCAAAGAATATCCTAAATTGAAGCAAAAAAAGGAAGACATGGAAAAGGATGAAGGAATTATATTT
GCTTTGATGTCTGGTTCAGGTAGTACATTATACAAAGTAATTTATGCTAACTGA

Upstream 100 bases:

>100_bases
TCGCGACATTATACCTTTTCCAATTGTAAAACCCATGGCTTTCGAAGACGAAGAAGCCATGTTAAAAGAAGAAGAGACAA
ACGAATGAAAGAGGAGTTAA

Downstream 100 bases:

>100_bases
ACTCTTTATCTAACTTAAAGACAAAAGCAATTATTTCAGCGGCCAATTCATACAATTCGGGAGGAATTTCTTCATCCAAA
TCTAAACCATAAAACTCTTC

Product: 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MHLKAFGKVNLYLDVISRRKDNYHNILTLFQSINEHDDIFIEFSESEIFESEPPLNISWDNNIIKKSIETFKKETGFDNF
NLKIKLIKNLPMGGGLGGGSADAAAVLNFLSKNFKISEKDLFEISCKIGSDVPFLLKGGTAIGKGKGEILEFLEPLKLNI
QIYPMNYNIDTKKMYEKLDQNWKSINHFGDPYNLYEALKNNDIITAKQNAFNVFEQVAFKEYPKLKQKKEDMEKDEGIIF
ALMSGSGSTLYKVIYAN

Sequences:

>Translated_257_residues
MHLKAFGKVNLYLDVISRRKDNYHNILTLFQSINEHDDIFIEFSESEIFESEPPLNISWDNNIIKKSIETFKKETGFDNF
NLKIKLIKNLPMGGGLGGGSADAAAVLNFLSKNFKISEKDLFEISCKIGSDVPFLLKGGTAIGKGKGEILEFLEPLKLNI
QIYPMNYNIDTKKMYEKLDQNWKSINHFGDPYNLYEALKNNDIITAKQNAFNVFEQVAFKEYPKLKQKKEDMEKDEGIIF
ALMSGSGSTLYKVIYAN
>Mature_257_residues
MHLKAFGKVNLYLDVISRRKDNYHNILTLFQSINEHDDIFIEFSESEIFESEPPLNISWDNNIIKKSIETFKKETGFDNF
NLKIKLIKNLPMGGGLGGGSADAAAVLNFLSKNFKISEKDLFEISCKIGSDVPFLLKGGTAIGKGKGEILEFLEPLKLNI
QIYPMNYNIDTKKMYEKLDQNWKSINHFGDPYNLYEALKNNDIITAKQNAFNVFEQVAFKEYPKLKQKKEDMEKDEGIIF
ALMSGSGSTLYKVIYAN

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=158, Percent_Identity=36.0759493670886, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_PETMO (A9BFC2)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001567230.1
- ProteinModelPortal:   A9BFC2
- SMR:   A9BFC2
- GeneID:   5756916
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_0160
- HOGENOM:   HBG734593
- OMA:   DGYHNID
- BioCyc:   PMOB403833:PMOB_0160-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376

Pfam domain/function: PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29321; Mature: 29321

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 131-131

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLKAFGKVNLYLDVISRRKDNYHNILTLFQSINEHDDIFIEFSESEIFESEPPLNISWD
CCCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCHHCCCCCCEEECCC
NNIIKKSIETFKKETGFDNFNLKIKLIKNLPMGGGLGGGSADAAAVLNFLSKNFKISEKD
CHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
LFEISCKIGSDVPFLLKGGTAIGKGKGEILEFLEPLKLNIQIYPMNYNIDTKKMYEKLDQ
EEEEEEECCCCCCEEEECCCEECCCHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHH
NWKSINHFGDPYNLYEALKNNDIITAKQNAFNVFEQVAFKEYPKLKQKKEDMEKDEGIIF
HHHHHHCCCCHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEE
ALMSGSGSTLYKVIYAN
EEECCCCCEEEEEEEEC
>Mature Secondary Structure
MHLKAFGKVNLYLDVISRRKDNYHNILTLFQSINEHDDIFIEFSESEIFESEPPLNISWD
CCCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCHHCCCCCCEEECCC
NNIIKKSIETFKKETGFDNFNLKIKLIKNLPMGGGLGGGSADAAAVLNFLSKNFKISEKD
CHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
LFEISCKIGSDVPFLLKGGTAIGKGKGEILEFLEPLKLNIQIYPMNYNIDTKKMYEKLDQ
EEEEEEECCCCCCEEEECCCEECCCHHHHHHHCCCEEEEEEEEEECCCCCHHHHHHHHHH
NWKSINHFGDPYNLYEALKNNDIITAKQNAFNVFEQVAFKEYPKLKQKKEDMEKDEGIIF
HHHHHHCCCCHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEE
ALMSGSGSTLYKVIYAN
EEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA