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The map label for this gene is iscS [H]

Identifier: 160901588

GI number: 160901588

Start: 95297

End: 96430

Strand: Direct

Name: iscS [H]

Synonym: Pmob_0097

Alternate gene names: 160901588

Gene position: 95297-96430 (Clockwise)

Preceding gene: 160901587

Following gene: 160901589

Centisome position: 4.39

GC content: 34.39

Gene sequence:

>1134_bases
GTGGTTTATTTCGACAACAACGCAACTACCCAAGTTGATAGAGAGGTTGCTGATTTGATTTTGAAATATATGACAGAATA
CTATGCAAATCCCAACTCTATTCATCAATTTGGAGTAAATGTTGAAAACGATTTAGAAGAAGCACGAGAGCAAATTTCCG
AGGTACTTAAAGTTTTACCCACGGAGATCTTTTTCACCTCTTGTGCTACTGAATCAATAAATTGGGTTATAAAAGGAGTT
GCTAAAGCCAACAAAAATAGAGGAAAACATATTGTAACCTCTACCATAGAGCATTCCGCTGTTATTAACACACTCAAGCA
ACTAGAAAGGGATGGCTTCGAAGTATCCTATATAAATGTGAATGATAAAGGAGTAATAGATCCAAATGAATTGTCAAAAA
ATATAAGACAAGATACCATACTAGTGAGCTTAATGGCTGCAAATAATGAAGTCGGTACCTTGCAACCTGTTGATGATGCC
TACAAAATCATAAAAGAAAAAAATGAAGAAACATACTTTCACATTGATGCTGTACAAGCTATTGGTAAAATTCCCTTTGA
TTTGTACAAATATAAATGTGATTTCGCTTCCTTTTCTGCACATAAATTTCATGGTCCAAAAGGTGTAGGTATTCTTTACA
AACGTAGAGGAACAAGAATATTCCCCTTCATAACAGGAGGTTCTCAAGAAAATGGGATGAGAGCAGGAACGCAAAATGTT
CCAGGAATCATAGGTACGGCTATGGCTTTGAAAAAATCTATTGAAAATCTTGACATAATGAATTCGAATATAAAAAATAT
TAGGGATCATCTAGCCGAAGAATTACTAAAAATGGGAGCAAAGATAGTTACTCCCCTAGAAAACTCCGTTCCAAATACAT
TAGCTTTTTTCTTTCCTAATATACGAGGAGATATTATTGTAAACGCCCTCTCAGAAGAAGAAATTTATGTATCAACCACT
TCTGCATGCTCAAGTAAGATTAAATCCTTCAGTAGAGTTATGGAAAGTATGGGATACAAAAATGACGAGGCAAGTGGTAT
GATACGAATTAGCTTATCTCACCTGAATAACGACGATGAGGCCGAATTATTCGTGATTAAGTTAAAAAATGTCTTAAAAT
TTTTGAATTATTGA

Upstream 100 bases:

>100_bases
GCTGATGCGTTAGATATTGAATTAAAAGCCTATTCTGAATTAGGTAAAGGTAGTACTTTTACCTTGATCTTTTTGAATCA
ACTGTGAGGAGGAAGCTAAC

Downstream 100 bases:

>100_bases
CTTTTTAATGTATAATAAACATAAAGGGGGCGAAATTATGCGAAGTATGACCGGTTATGGGAGATTAACAAAAAATATTG
GCGATTATAGCTATAATGTA

Product: class V aminotransferase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MVYFDNNATTQVDREVADLILKYMTEYYANPNSIHQFGVNVENDLEEAREQISEVLKVLPTEIFFTSCATESINWVIKGV
AKANKNRGKHIVTSTIEHSAVINTLKQLERDGFEVSYINVNDKGVIDPNELSKNIRQDTILVSLMAANNEVGTLQPVDDA
YKIIKEKNEETYFHIDAVQAIGKIPFDLYKYKCDFASFSAHKFHGPKGVGILYKRRGTRIFPFITGGSQENGMRAGTQNV
PGIIGTAMALKKSIENLDIMNSNIKNIRDHLAEELLKMGAKIVTPLENSVPNTLAFFFPNIRGDIIVNALSEEEIYVSTT
SACSSKIKSFSRVMESMGYKNDEASGMIRISLSHLNNDDEAELFVIKLKNVLKFLNY

Sequences:

>Translated_377_residues
MVYFDNNATTQVDREVADLILKYMTEYYANPNSIHQFGVNVENDLEEAREQISEVLKVLPTEIFFTSCATESINWVIKGV
AKANKNRGKHIVTSTIEHSAVINTLKQLERDGFEVSYINVNDKGVIDPNELSKNIRQDTILVSLMAANNEVGTLQPVDDA
YKIIKEKNEETYFHIDAVQAIGKIPFDLYKYKCDFASFSAHKFHGPKGVGILYKRRGTRIFPFITGGSQENGMRAGTQNV
PGIIGTAMALKKSIENLDIMNSNIKNIRDHLAEELLKMGAKIVTPLENSVPNTLAFFFPNIRGDIIVNALSEEEIYVSTT
SACSSKIKSFSRVMESMGYKNDEASGMIRISLSHLNNDDEAELFVIKLKNVLKFLNY
>Mature_377_residues
MVYFDNNATTQVDREVADLILKYMTEYYANPNSIHQFGVNVENDLEEAREQISEVLKVLPTEIFFTSCATESINWVIKGV
AKANKNRGKHIVTSTIEHSAVINTLKQLERDGFEVSYINVNDKGVIDPNELSKNIRQDTILVSLMAANNEVGTLQPVDDA
YKIIKEKNEETYFHIDAVQAIGKIPFDLYKYKCDFASFSAHKFHGPKGVGILYKRRGTRIFPFITGGSQENGMRAGTQNV
PGIIGTAMALKKSIENLDIMNSNIKNIRDHLAEELLKMGAKIVTPLENSVPNTLAFFFPNIRGDIIVNALSEEEIYVSTT
SACSSKIKSFSRVMESMGYKNDEASGMIRISLSHLNNDDEAELFVIKLKNVLKFLNY

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=381, Percent_Identity=36.2204724409449, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=30.9927360774818, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI48994898, Length=378, Percent_Identity=38.6243386243386, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1787970, Length=408, Percent_Identity=26.7156862745098, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789175, Length=400, Percent_Identity=24.75, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=365, Percent_Identity=36.7123287671233, Blast_Score=238, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI17533177, Length=325, Percent_Identity=33.2307692307692, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=379, Percent_Identity=36.4116094986807, Blast_Score=245, Evalue=1e-65,
Organism=Drosophila melanogaster, GI20129463, Length=373, Percent_Identity=36.1930294906166, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42312; Mature: 42312

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYFDNNATTQVDREVADLILKYMTEYYANPNSIHQFGVNVENDLEEAREQISEVLKVLP
CEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
TEIFFTSCATESINWVIKGVAKANKNRGKHIVTSTIEHSAVINTLKQLERDGFEVSYINV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEE
NDKGVIDPNELSKNIRQDTILVSLMAANNEVGTLQPVDDAYKIIKEKNEETYFHIDAVQA
CCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHH
IGKIPFDLYKYKCDFASFSAHKFHGPKGVGILYKRRGTRIFPFITGGSQENGMRAGTQNV
HCCCCHHHHHEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCC
PGIIGTAMALKKSIENLDIMNSNIKNIRDHLAEELLKMGAKIVTPLENSVPNTLAFFFPN
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEECCCC
IRGDIIVNALSEEEIYVSTTSACSSKIKSFSRVMESMGYKNDEASGMIRISLSHLNNDDE
CCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCC
AELFVIKLKNVLKFLNY
HHEEEEEHHHHHHHHCC
>Mature Secondary Structure
MVYFDNNATTQVDREVADLILKYMTEYYANPNSIHQFGVNVENDLEEAREQISEVLKVLP
CEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHH
TEIFFTSCATESINWVIKGVAKANKNRGKHIVTSTIEHSAVINTLKQLERDGFEVSYINV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEE
NDKGVIDPNELSKNIRQDTILVSLMAANNEVGTLQPVDDAYKIIKEKNEETYFHIDAVQA
CCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHH
IGKIPFDLYKYKCDFASFSAHKFHGPKGVGILYKRRGTRIFPFITGGSQENGMRAGTQNV
HCCCCHHHHHEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCC
PGIIGTAMALKKSIENLDIMNSNIKNIRDHLAEELLKMGAKIVTPLENSVPNTLAFFFPN
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCEEECCCC
IRGDIIVNALSEEEIYVSTTSACSSKIKSFSRVMESMGYKNDEASGMIRISLSHLNNDDE
CCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCC
AELFVIKLKNVLKFLNY
HHEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]