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The map label for this gene is rimO

Identifier: 160901569

GI number: 160901569

Start: 75928

End: 77235

Strand: Direct

Name: rimO

Synonym: Pmob_0078

Alternate gene names: 160901569

Gene position: 75928-77235 (Clockwise)

Preceding gene: 160901568

Following gene: 160901570

Centisome position: 3.5

GC content: 31.65

Gene sequence:

>1308_bases
ATGAAAAAGTTTCACATCGTAAAATTAGGGTGTCCAAAAAATGATGCAGATATGGAAATATTCAAAGGGTTGCTCCAAAG
CAAGGGGTATAAATACGAGAGTAATCCTCAATTAGCTAATTATATTTTCATCGATACATGTGGTTTCATCGAAGAAGCTA
AAAAAGAAAGCATCGAAACCATATTTGAATATGTATCCCTAAAAGATAATAACAAAAACCTAAAAGTTATACCAATAGGT
TGTCTAACTCAAAGATATTTCGACGATATTCTTAAAGACATCCCTGAGATTGATGGATTATATGGTGTGTTATCTCCAAA
AACCATAGTTGAAAAAATTGAAAATGGTGAATATTTTTTTAAAAGGGATATTCCTGAAACTCTGTACGATTGTAAAATTA
GAGCGATTCCAGACTCACACTACGCATACGTAAAAATCGGAGATGGATGTAGTAGAAATTGTGCTTTTTGCTCTATACCC
ACTTTCAAAGGGAAACCAAAAAGCAGAAGTATCGAAGAAATAAACGAAGAAGTTGAATTTTTGGTATCCAAAGGTGTAAA
AGAAATCATTCTCGTATCCCAAGACAATACCTTGTACGGCATAGACAATTACCAAAAGCAGGCATTACCGGATTTACTAG
ACAAACTTAACAATATAAAAGGAAAATTTTGGATCAGGGTGATGTACTTACATCCGGATTTTTTAAGTGAAGAAATCATA
GAAAGCATACATAGAAATGAAAAAGTATTAAACTATTTCGATGTTCCAATTCAACATATCTCTGATAAAATTTTGCAAAG
TATGGGTAGACATAAGAAAAGAAATGAATTAATTAAACTATTTGAGAAAATCAGAAAAGAACCATCTGCCATAAGAACCA
CGTTAATGGTAGGGTTCCCAGGAGAAAAAGCTGAAGATTTTGAAGAATTAGTAGATTTTGTAAAAGAGATAAAATTTGAA
AGAATGGGCAGTTTTATTTTCTCAAAAGAAGAAAACACCAAGTCATTCACATTACCAGAACAAATTGATGAACAAATAAA
AAAACAAAGGCAAAATGAACTAATGACTGTTCAAAGTGAAATTTCTAAAAACATCATGGAAAAATATATAGGTGAAACTT
TAGAAGTTCTTTTGGAAGAAAAAGAAGACAACGTTTATGTCGGAAGAAGCTATTTAGACGCTCCAGAGATTGATGGGAAT
GTATATATAAAAAATTTTGGTGACAAAGAACTAACTTTTGGGAATTTTGTCAAGGTGACAATTACTGGTTCATACGAATA
TGATCTGGAAGGAGAAATAGTTGAATGA

Upstream 100 bases:

>100_bases
AAAATTCTTTTATCCTTATGGGTGGGGAGCGGGGCGAAGGGGCGCTATATAATTAGATTTTAAAAGAAATATATTATAAT
GAAATCATCGGAGGAAGCGA

Downstream 100 bases:

>100_bases
ACATACCAAATTTATTGAGTTTTTCTAGGATAATATTAGCCATTCCAATATTCATCCTAACTGTCTTTGGAGAACAGTTT
TATACCGCAGCCTTGGTTTT

Product: MiaB-like tRNA modifying protein YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKKFHIVKLGCPKNDADMEIFKGLLQSKGYKYESNPQLANYIFIDTCGFIEEAKKESIETIFEYVSLKDNNKNLKVIPIG
CLTQRYFDDILKDIPEIDGLYGVLSPKTIVEKIENGEYFFKRDIPETLYDCKIRAIPDSHYAYVKIGDGCSRNCAFCSIP
TFKGKPKSRSIEEINEEVEFLVSKGVKEIILVSQDNTLYGIDNYQKQALPDLLDKLNNIKGKFWIRVMYLHPDFLSEEII
ESIHRNEKVLNYFDVPIQHISDKILQSMGRHKKRNELIKLFEKIRKEPSAIRTTLMVGFPGEKAEDFEELVDFVKEIKFE
RMGSFIFSKEENTKSFTLPEQIDEQIKKQRQNELMTVQSEISKNIMEKYIGETLEVLLEEKEDNVYVGRSYLDAPEIDGN
VYIKNFGDKELTFGNFVKVTITGSYEYDLEGEIVE

Sequences:

>Translated_435_residues
MKKFHIVKLGCPKNDADMEIFKGLLQSKGYKYESNPQLANYIFIDTCGFIEEAKKESIETIFEYVSLKDNNKNLKVIPIG
CLTQRYFDDILKDIPEIDGLYGVLSPKTIVEKIENGEYFFKRDIPETLYDCKIRAIPDSHYAYVKIGDGCSRNCAFCSIP
TFKGKPKSRSIEEINEEVEFLVSKGVKEIILVSQDNTLYGIDNYQKQALPDLLDKLNNIKGKFWIRVMYLHPDFLSEEII
ESIHRNEKVLNYFDVPIQHISDKILQSMGRHKKRNELIKLFEKIRKEPSAIRTTLMVGFPGEKAEDFEELVDFVKEIKFE
RMGSFIFSKEENTKSFTLPEQIDEQIKKQRQNELMTVQSEISKNIMEKYIGETLEVLLEEKEDNVYVGRSYLDAPEIDGN
VYIKNFGDKELTFGNFVKVTITGSYEYDLEGEIVE
>Mature_435_residues
MKKFHIVKLGCPKNDADMEIFKGLLQSKGYKYESNPQLANYIFIDTCGFIEEAKKESIETIFEYVSLKDNNKNLKVIPIG
CLTQRYFDDILKDIPEIDGLYGVLSPKTIVEKIENGEYFFKRDIPETLYDCKIRAIPDSHYAYVKIGDGCSRNCAFCSIP
TFKGKPKSRSIEEINEEVEFLVSKGVKEIILVSQDNTLYGIDNYQKQALPDLLDKLNNIKGKFWIRVMYLHPDFLSEEII
ESIHRNEKVLNYFDVPIQHISDKILQSMGRHKKRNELIKLFEKIRKEPSAIRTTLMVGFPGEKAEDFEELVDFVKEIKFE
RMGSFIFSKEENTKSFTLPEQIDEQIKKQRQNELMTVQSEISKNIMEKYIGETLEVLLEEKEDNVYVGRSYLDAPEIDGN
VYIKNFGDKELTFGNFVKVTITGSYEYDLEGEIVE

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=486, Percent_Identity=25.5144032921811, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI28872782, Length=486, Percent_Identity=25.5144032921811, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI93277076, Length=405, Percent_Identity=28.3950617283951, Blast_Score=116, Evalue=5e-26,
Organism=Escherichia coli, GI1787057, Length=452, Percent_Identity=33.8495575221239, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1786882, Length=449, Percent_Identity=28.2850779510022, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=470, Percent_Identity=24.2553191489362, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71996771, Length=243, Percent_Identity=27.1604938271605, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21356207, Length=479, Percent_Identity=23.3820459290188, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922432, Length=456, Percent_Identity=25, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_PETMO (A9BEU9)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001567150.1
- GeneID:   5757148
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_0078
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- BioCyc:   PMOB403833:PMOB_0078-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50507; Mature: 50507

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFHIVKLGCPKNDADMEIFKGLLQSKGYKYESNPQLANYIFIDTCGFIEEAKKESIET
CCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
IFEYVSLKDNNKNLKVIPIGCLTQRYFDDILKDIPEIDGLYGVLSPKTIVEKIENGEYFF
HHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCHHHCCHHHCCCHHHHHHHHCCCCEEE
KRDIPETLYDCKIRAIPDSHYAYVKIGDGCSRNCAFCSIPTFKGKPKSRSIEEINEEVEF
ECCCHHHHHCCEEEECCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
LVSKGVKEIILVSQDNTLYGIDNYQKQALPDLLDKLNNIKGKFWIRVMYLHPDFLSEEII
HHHCCCEEEEEEECCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHH
ESIHRNEKVLNYFDVPIQHISDKILQSMGRHKKRNELIKLFEKIRKEPSAIRTTLMVGFP
HHHHCCCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHEEEEEEECC
GEKAEDFEELVDFVKEIKFERMGSFIFSKEENTKSFTLPEQIDEQIKKQRQNELMTVQSE
CCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ISKNIMEKYIGETLEVLLEEKEDNVYVGRSYLDAPEIDGNVYIKNFGDKELTFGNFVKVT
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEEE
ITGSYEYDLEGEIVE
EECCEEECCCCCCCC
>Mature Secondary Structure
MKKFHIVKLGCPKNDADMEIFKGLLQSKGYKYESNPQLANYIFIDTCGFIEEAKKESIET
CCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
IFEYVSLKDNNKNLKVIPIGCLTQRYFDDILKDIPEIDGLYGVLSPKTIVEKIENGEYFF
HHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCHHHCCHHHCCCHHHHHHHHCCCCEEE
KRDIPETLYDCKIRAIPDSHYAYVKIGDGCSRNCAFCSIPTFKGKPKSRSIEEINEEVEF
ECCCHHHHHCCEEEECCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHH
LVSKGVKEIILVSQDNTLYGIDNYQKQALPDLLDKLNNIKGKFWIRVMYLHPDFLSEEII
HHHCCCEEEEEEECCCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHH
ESIHRNEKVLNYFDVPIQHISDKILQSMGRHKKRNELIKLFEKIRKEPSAIRTTLMVGFP
HHHHCCCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHEEEEEEECC
GEKAEDFEELVDFVKEIKFERMGSFIFSKEENTKSFTLPEQIDEQIKKQRQNELMTVQSE
CCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ISKNIMEKYIGETLEVLLEEKEDNVYVGRSYLDAPEIDGNVYIKNFGDKELTFGNFVKVT
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCEEEEE
ITGSYEYDLEGEIVE
EECCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA