Click here to switch to the map view.

The map label for this gene is polA [H]

Identifier: 160901568

GI number: 160901568

Start: 72964

End: 75654

Strand: Direct

Name: polA [H]

Synonym: Pmob_0077

Alternate gene names: 160901568

Gene position: 72964-75654 (Clockwise)

Preceding gene: 160901567

Following gene: 160901569

Centisome position: 3.36

GC content: 33.0

Gene sequence:

>2691_bases
ATGGCAAATTTATACTTAATAGACGGCTCAGGAATTGCATATAGAGCCTTTTTTGCACTCGGGGACTGGATGAGTACTTC
TGATGGTTTGCCGACAAACGCCATTTATGGTGTAGCTAGAATGCTTCTAAAGTTACTCAAAGAGTACGTTAAAAAAGGTG
AAGATTCAATAATATTTGTTATGGACAAAAAAACCACCACATATAGACATGAATTATTAGAAAGCTATAAAGCCCAGAGA
CCCGAAACACCCGAAAAATATATACAACAGATCCCATACATATACGAAATGGTTGAAAAATTAGGCATAAAATTAGTTGC
CATGGATAATTACGAAGCAGACGATGTTATAGCCACGATAGTGTCCAAAAAGAAAAGAAACTACGATAATGTTTATATAA
TTACCTCGGATAAAGATATGATGCAACTTGTTAAGGATAACGTATATATCTTAAGACCAGAACAAGGAATAACAGAAATG
GTGAAATACGATGCACAGCAAGTAGAAAAAAAGATGGGAGTCCCTCCAGAAAAAATTGCAGATTTACTAGCTTTAATGGG
GGATAGTTCTGACAATATCCCAGGTGTAAAAGGTATTGGAATAAAAACAGCCCAAAAATTGCTGCAAGATTACGATAACA
TAGACGATCTTTATCAACATTTGGATGAGATTAAAGGTTCAACAAAAAACAAGCTAAAAAATGAAAAGGAAACCGCATAT
CTGAGTAAAAAACTAGTCCAATTGATGGTAGATGCCCCCATAGAAGAAATTTTTGAAGACAAAGAAATTATATATCAAGG
ATTCAGAAACAATCTACGAGATTTTCTCAAGAAATTAGATTTCAATTCAATATTAAAAGAGTTAGATATGCCTGATACAA
CTACTAACTCAACTAAAGTTGCAGCAGAGACAAAAGCAGAAAAAAAAGATTATTCGACGAAAGGTAAGTATTACGAATAC
AACGCACAAGATTACAAAGAACTATTAAAAATCTTAGAGAAATACGAAATTATATCTTTTGACCTTGAAACTTCTTCTCT
TGATCCATATCAAGCAGATATAGTGGGAATCGCTTTAAGCTGCAAACCATTTGAAGGATATTTTTTGTATCTATACAAAG
AAAAAGACAGGTGGGAAATAACGAAAGAGATAGTCGATCTTTTAAACAATAAAAAGGTCATTGGTCAAAATTTAAAATAC
GATATCACCGTTTTGAAAGTAAATGGAATTGAATTAAGCAAAGTACATTTTGACAGTATGATAGCCGCTTATTTGTTGAA
TCCAGATAGTAGAAGGTTCAATATGGATGATCTTGCCAAAGAATATTTGGATTATAAAACAATTAAATACAAAGAAGTGA
TGGGAAAAGATATCAAGCTGTTAACCCTTGGAGACATCGATAAAAAAAGGGTCGTTGAGTACGCTGGAGAAGATGCTGAT
ATTGCTTATCGATTATTTGAAGTATTAAAGCCTAAATTAGAAGAATTTGAACTATTTGAATTGTTTGAAAAAATCGAAAT
GTCAACTATAAATGTTTTATCAGAAATGGAAATGAACGGCGTGTATTTTGATTTAAAAGAACTACAAAAATTGGAAGAAG
AATACAACAAAAAGCTAAGTTCGTTAATGTATGAAATGAAAAAAATCGTCGGTTATGACTTCAATCCAAATTCGCCAAAA
CAAGTTGGAGAACTACTTTTTGAAAACTTAGGATTGAAAGGAAAAAGAAAAACTAAGAGTGGATCATATTCAACAGATGC
AGATTCTTTAGAAGCCCTAAGAGATGAACATCCAATAATAGAAAAGCTCTTAGAATACAGAAAATACCAAAAACTTCTAT
CTACATACATAATCGCTATACCAAAACTAGTTAACAAAAAAACTGGAAGGGTTCACACATCTTTCAACCAAACTGGAACA
GCTACCGGTAGATTAAGTAGCAGCGAACCGAATCTTCAAAATCTCCCCATACGAGAAGAAGATGGAGAAAGAATAAGGAG
CACCGTTAAGGCACAAAAAGATGATTATGTGCTACTGAGTGCCGATTATTCTCAAATTGAATTGAGGGTTTTAGCTCATC
TAACTAACGATGAAACTTTAATAAATGCTTTTAACAATGGTGAAGACATACATGCTTTAACCGCAGCTGCAATATTCGGT
GTAAAAATCGACGATGTTGACTACAACATGAGAAGGGTAGGAAAAGTAGTTAATTTTTCCCTGGTGTATGGATCATCTCC
GTATGGATTAGCCGAGAATTTAAAGATACCTGTCGAAGATGCAAAAGATTTCATGAACAGATACTTTAAAACTTACCAAA
AAGTCAAAGAATATCAAGAATCAAGTTTAAAGATTGCAACGCAAAAAGGCTACGTTGAAACCATATTTGGAAGGAAAAGA
TTTTTGAAAAACATAAAAACAGGTAAATCAGAGTTGAAACGAATAGTCATAAATACCCCTATCCAAGGTAGTGCTGCAGA
TATCATGAAATTGGCGATGATCAATTTATACAAAAAACTTCCAAAAGAAGCAAAATTGATACTTCAGGTGCACGATGAAG
TTGTAATAGAATTACCTGAAAAAATCGTAGAAGAAACCAAAAAAATGGTTCAAGATTGTATGGAAAATGCGGTCAAATTG
AATATACCTTTAAAAGTAGATATCAGTGTCGGAAAAAATTGGCAAAAATGA

Upstream 100 bases:

>100_bases
GATGTAAAAATCGTGGAAGATATCAAGCCAGATGAAAAAACCGGAAAATATAAATTAATTGTAATTTAGTAAAAAATCAA
AAGAAAAGAAGGTGCTTCAC

Downstream 100 bases:

>100_bases
TAATTATAATTATGTATAATTAAATTATTAAAATTTTTTCATTTTTTCAAGAAAATCTGATATAATCTAAATAACTATTT
AAACATTCATAGATAAAAAG

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 896; Mature: 895

Protein sequence:

>896_residues
MANLYLIDGSGIAYRAFFALGDWMSTSDGLPTNAIYGVARMLLKLLKEYVKKGEDSIIFVMDKKTTTYRHELLESYKAQR
PETPEKYIQQIPYIYEMVEKLGIKLVAMDNYEADDVIATIVSKKKRNYDNVYIITSDKDMMQLVKDNVYILRPEQGITEM
VKYDAQQVEKKMGVPPEKIADLLALMGDSSDNIPGVKGIGIKTAQKLLQDYDNIDDLYQHLDEIKGSTKNKLKNEKETAY
LSKKLVQLMVDAPIEEIFEDKEIIYQGFRNNLRDFLKKLDFNSILKELDMPDTTTNSTKVAAETKAEKKDYSTKGKYYEY
NAQDYKELLKILEKYEIISFDLETSSLDPYQADIVGIALSCKPFEGYFLYLYKEKDRWEITKEIVDLLNNKKVIGQNLKY
DITVLKVNGIELSKVHFDSMIAAYLLNPDSRRFNMDDLAKEYLDYKTIKYKEVMGKDIKLLTLGDIDKKRVVEYAGEDAD
IAYRLFEVLKPKLEEFELFELFEKIEMSTINVLSEMEMNGVYFDLKELQKLEEEYNKKLSSLMYEMKKIVGYDFNPNSPK
QVGELLFENLGLKGKRKTKSGSYSTDADSLEALRDEHPIIEKLLEYRKYQKLLSTYIIAIPKLVNKKTGRVHTSFNQTGT
ATGRLSSSEPNLQNLPIREEDGERIRSTVKAQKDDYVLLSADYSQIELRVLAHLTNDETLINAFNNGEDIHALTAAAIFG
VKIDDVDYNMRRVGKVVNFSLVYGSSPYGLAENLKIPVEDAKDFMNRYFKTYQKVKEYQESSLKIATQKGYVETIFGRKR
FLKNIKTGKSELKRIVINTPIQGSAADIMKLAMINLYKKLPKEAKLILQVHDEVVIELPEKIVEETKKMVQDCMENAVKL
NIPLKVDISVGKNWQK

Sequences:

>Translated_896_residues
MANLYLIDGSGIAYRAFFALGDWMSTSDGLPTNAIYGVARMLLKLLKEYVKKGEDSIIFVMDKKTTTYRHELLESYKAQR
PETPEKYIQQIPYIYEMVEKLGIKLVAMDNYEADDVIATIVSKKKRNYDNVYIITSDKDMMQLVKDNVYILRPEQGITEM
VKYDAQQVEKKMGVPPEKIADLLALMGDSSDNIPGVKGIGIKTAQKLLQDYDNIDDLYQHLDEIKGSTKNKLKNEKETAY
LSKKLVQLMVDAPIEEIFEDKEIIYQGFRNNLRDFLKKLDFNSILKELDMPDTTTNSTKVAAETKAEKKDYSTKGKYYEY
NAQDYKELLKILEKYEIISFDLETSSLDPYQADIVGIALSCKPFEGYFLYLYKEKDRWEITKEIVDLLNNKKVIGQNLKY
DITVLKVNGIELSKVHFDSMIAAYLLNPDSRRFNMDDLAKEYLDYKTIKYKEVMGKDIKLLTLGDIDKKRVVEYAGEDAD
IAYRLFEVLKPKLEEFELFELFEKIEMSTINVLSEMEMNGVYFDLKELQKLEEEYNKKLSSLMYEMKKIVGYDFNPNSPK
QVGELLFENLGLKGKRKTKSGSYSTDADSLEALRDEHPIIEKLLEYRKYQKLLSTYIIAIPKLVNKKTGRVHTSFNQTGT
ATGRLSSSEPNLQNLPIREEDGERIRSTVKAQKDDYVLLSADYSQIELRVLAHLTNDETLINAFNNGEDIHALTAAAIFG
VKIDDVDYNMRRVGKVVNFSLVYGSSPYGLAENLKIPVEDAKDFMNRYFKTYQKVKEYQESSLKIATQKGYVETIFGRKR
FLKNIKTGKSELKRIVINTPIQGSAADIMKLAMINLYKKLPKEAKLILQVHDEVVIELPEKIVEETKKMVQDCMENAVKL
NIPLKVDISVGKNWQK
>Mature_895_residues
ANLYLIDGSGIAYRAFFALGDWMSTSDGLPTNAIYGVARMLLKLLKEYVKKGEDSIIFVMDKKTTTYRHELLESYKAQRP
ETPEKYIQQIPYIYEMVEKLGIKLVAMDNYEADDVIATIVSKKKRNYDNVYIITSDKDMMQLVKDNVYILRPEQGITEMV
KYDAQQVEKKMGVPPEKIADLLALMGDSSDNIPGVKGIGIKTAQKLLQDYDNIDDLYQHLDEIKGSTKNKLKNEKETAYL
SKKLVQLMVDAPIEEIFEDKEIIYQGFRNNLRDFLKKLDFNSILKELDMPDTTTNSTKVAAETKAEKKDYSTKGKYYEYN
AQDYKELLKILEKYEIISFDLETSSLDPYQADIVGIALSCKPFEGYFLYLYKEKDRWEITKEIVDLLNNKKVIGQNLKYD
ITVLKVNGIELSKVHFDSMIAAYLLNPDSRRFNMDDLAKEYLDYKTIKYKEVMGKDIKLLTLGDIDKKRVVEYAGEDADI
AYRLFEVLKPKLEEFELFELFEKIEMSTINVLSEMEMNGVYFDLKELQKLEEEYNKKLSSLMYEMKKIVGYDFNPNSPKQ
VGELLFENLGLKGKRKTKSGSYSTDADSLEALRDEHPIIEKLLEYRKYQKLLSTYIIAIPKLVNKKTGRVHTSFNQTGTA
TGRLSSSEPNLQNLPIREEDGERIRSTVKAQKDDYVLLSADYSQIELRVLAHLTNDETLINAFNNGEDIHALTAAAIFGV
KIDDVDYNMRRVGKVVNFSLVYGSSPYGLAENLKIPVEDAKDFMNRYFKTYQKVKEYQESSLKIATQKGYVETIFGRKRF
LKNIKTGKSELKRIVINTPIQGSAADIMKLAMINLYKKLPKEAKLILQVHDEVVIELPEKIVEETKKMVQDCMENAVKLN
IPLKVDISVGKNWQK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=519, Percent_Identity=30.0578034682081, Blast_Score=237, Evalue=4e-62,
Organism=Homo sapiens, GI139394648, Length=684, Percent_Identity=25.8771929824561, Blast_Score=204, Evalue=3e-52,
Organism=Escherichia coli, GI1790294, Length=930, Percent_Identity=39.5698924731183, Blast_Score=613, Evalue=1e-176,
Organism=Escherichia coli, GI226510970, Length=198, Percent_Identity=30.3030303030303, Blast_Score=112, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI86563272, Length=425, Percent_Identity=30.1176470588235, Blast_Score=202, Evalue=7e-52,
Organism=Drosophila melanogaster, GI17933644, Length=422, Percent_Identity=31.9905213270142, Blast_Score=197, Evalue=4e-50,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 103186; Mature: 103055

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLYLIDGSGIAYRAFFALGDWMSTSDGLPTNAIYGVARMLLKLLKEYVKKGEDSIIFV
CCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
MDKKTTTYRHELLESYKAQRPETPEKYIQQIPYIYEMVEKLGIKLVAMDNYEADDVIATI
ECCCCHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHCEEEEEECCCCHHHHHHHH
VSKKKRNYDNVYIITSDKDMMQLVKDNVYILRPEQGITEMVKYDAQQVEKKMGVPPEKIA
HHHHCCCCCCEEEEECCHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHH
DLLALMGDSSDNIPGVKGIGIKTAQKLLQDYDNIDDLYQHLDEIKGSTKNKLKNEKETAY
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCHHHHHH
LSKKLVQLMVDAPIEEIFEDKEIIYQGFRNNLRDFLKKLDFNSILKELDMPDTTTNSTKV
HHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCEE
AAETKAEKKDYSTKGKYYEYNAQDYKELLKILEKYEIISFDLETSSLDPYQADIVGIALS
HHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHCEEEEEEE
CKPFEGYFLYLYKEKDRWEITKEIVDLLNNKKVIGQNLKYDITVLKVNGIELSKVHFDSM
ECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEECCCCEEEEEEEEECCEEEEHHHHHHH
IAAYLLNPDSRRFNMDDLAKEYLDYKTIKYKEVMGKDIKLLTLGDIDKKRVVEYAGEDAD
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCHH
IAYRLFEVLKPKLEEFELFELFEKIEMSTINVLSEMEMNGVYFDLKELQKLEEEYNKKLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHH
SLMYEMKKIVGYDFNPNSPKQVGELLFENLGLKGKRKTKSGSYSTDADSLEALRDEHPII
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHH
EKLLEYRKYQKLLSTYIIAIPKLVNKKTGRVHTSFNQTGTATGRLSSSEPNLQNLPIREE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
DGERIRSTVKAQKDDYVLLSADYSQIELRVLAHLTNDETLINAFNNGEDIHALTAAAIFG
CHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHC
VKIDDVDYNMRRVGKVVNFSLVYGSSPYGLAENLKIPVEDAKDFMNRYFKTYQKVKEYQE
CEEECCCCCHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SSLKIATQKGYVETIFGRKRFLKNIKTGKSELKRIVINTPIQGSAADIMKLAMINLYKKL
CCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHEEHCCCCCCCCHHHHHHHHHHHHHHHC
PKEAKLILQVHDEVVIELPEKIVEETKKMVQDCMENAVKLNIPLKVDISVGKNWQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEEECCCCCCC
>Mature Secondary Structure 
ANLYLIDGSGIAYRAFFALGDWMSTSDGLPTNAIYGVARMLLKLLKEYVKKGEDSIIFV
CEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
MDKKTTTYRHELLESYKAQRPETPEKYIQQIPYIYEMVEKLGIKLVAMDNYEADDVIATI
ECCCCHHHHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHCEEEEEECCCCHHHHHHHH
VSKKKRNYDNVYIITSDKDMMQLVKDNVYILRPEQGITEMVKYDAQQVEKKMGVPPEKIA
HHHHCCCCCCEEEEECCHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHH
DLLALMGDSSDNIPGVKGIGIKTAQKLLQDYDNIDDLYQHLDEIKGSTKNKLKNEKETAY
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCHHHHHH
LSKKLVQLMVDAPIEEIFEDKEIIYQGFRNNLRDFLKKLDFNSILKELDMPDTTTNSTKV
HHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCEE
AAETKAEKKDYSTKGKYYEYNAQDYKELLKILEKYEIISFDLETSSLDPYQADIVGIALS
HHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHCEEEEEEE
CKPFEGYFLYLYKEKDRWEITKEIVDLLNNKKVIGQNLKYDITVLKVNGIELSKVHFDSM
ECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEECCCCEEEEEEEEECCEEEEHHHHHHH
IAAYLLNPDSRRFNMDDLAKEYLDYKTIKYKEVMGKDIKLLTLGDIDKKRVVEYAGEDAD
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCHH
IAYRLFEVLKPKLEEFELFELFEKIEMSTINVLSEMEMNGVYFDLKELQKLEEEYNKKLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHH
SLMYEMKKIVGYDFNPNSPKQVGELLFENLGLKGKRKTKSGSYSTDADSLEALRDEHPII
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHH
EKLLEYRKYQKLLSTYIIAIPKLVNKKTGRVHTSFNQTGTATGRLSSSEPNLQNLPIREE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
DGERIRSTVKAQKDDYVLLSADYSQIELRVLAHLTNDETLINAFNNGEDIHALTAAAIFG
CHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHC
VKIDDVDYNMRRVGKVVNFSLVYGSSPYGLAENLKIPVEDAKDFMNRYFKTYQKVKEYQE
CEEECCCCCHHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SSLKIATQKGYVETIFGRKRFLKNIKTGKSELKRIVINTPIQGSAADIMKLAMINLYKKL
CCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHEEHCCCCCCCCHHHHHHHHHHHHHHHC
PKEAKLILQVHDEVVIELPEKIVEETKKMVQDCMENAVKLNIPLKVDISVGKNWQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6276402; 8346018; 9278503; 6183253; 6302278; 7035456; 3883192; 1989886; 8469987; 8260491; 9514742; 9888810; 10588690; 8442659 [H]