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The map label for this gene is insO2 [H]

Identifier: 160877515

GI number: 160877515

Start: 5214759

End: 5215571

Strand: Reverse

Name: insO2 [H]

Synonym: Sbal195_4414

Alternate gene names: 160877515

Gene position: 5215571-5214759 (Counterclockwise)

Preceding gene: 160877516

Following gene: 160877514

Centisome position: 97.54

GC content: 42.8

Gene sequence:

>813_bases
GTGCATCGAAGTAGCTATAAATATTGGCTAAAAAGAAAAGAACATATCGATGCTGATTTTACAATTTTATGCAGTGAAGT
GAAGGCCGCGCATCGTATTAGTCATGGCTCTGCCGGTGCTCGAACGATAGCGCAATTAGTGACTAACAAAGGGATCTGTC
TCAGTCGTTATCGAGCCCGTAATCTAATGAAAAAGCTTGGGTTATTGAGCTGTCAGCAGCCTAAACATTCTTATAGGAAA
GCTGCACAAGAGCATGTAGCGATCCCGAATACGCTTAATCGACAGTTTGCCGTAACTCAACCAGATCAAGTGTGGTGTGG
CGATGTGACGTATGTTTGGGTCGGTAATCGTTGGGCATATTTAGCGGTTGTTCTAGACCTGTTTTCCCGTAAACCTGTTG
GGTGGGCTTTGTCATTATCACCTGACAGCGAGCTGACCTGTAAAGCCTTGAAAATGGCATTTGAGTTAAGGGGGAAACCT
GAGAATGTGATTTATCACAGCGATCAAGGTTCACATTACACAAGCTTGAAATTCAGACAATTAATATGGCGTCTTCAGAT
TGAGCAAAGTATGAGTCGCCGCGGGAATTGTTGGGATAACGCACCAATGGAGCGTTTCTTTAGAAGTTTGAAATCTGAGT
GGATACCCGCCAGTGGTTATGGGAATTTTACAGAAGCAGATAAAGAGATCACAAATTACATCACTGGATATTACAGTGAG
ACCAGACCCCATCAATATAATGGTGGGTTAACGCCAAATGAGTCAGAACGTTTATATTGGAATGACTCTAAAACCGTGGC
CAATTTTACTTGA

Upstream 100 bases:

>100_bases
AGGCTACCGCTCTCTTGATGTCAGACTCGATGAACAATTTACGCTGATCAGCGCACTCAAGCAGAGCCACTCTATTCTCA
CAATTTGTAATGCATTCAAA

Downstream 100 bases:

>100_bases
CCACTACAAATGCATTAACTGTTGGTGCATACACACAGAAAACTCATATCACTGAAGAAGATACTCAAGCATATTCATCA
CTAGCTCCGCATGGTGGATT

Product: integrase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MHRSSYKYWLKRKEHIDADFTILCSEVKAAHRISHGSAGARTIAQLVTNKGICLSRYRARNLMKKLGLLSCQQPKHSYRK
AAQEHVAIPNTLNRQFAVTQPDQVWCGDVTYVWVGNRWAYLAVVLDLFSRKPVGWALSLSPDSELTCKALKMAFELRGKP
ENVIYHSDQGSHYTSLKFRQLIWRLQIEQSMSRRGNCWDNAPMERFFRSLKSEWIPASGYGNFTEADKEITNYITGYYSE
TRPHQYNGGLTPNESERLYWNDSKTVANFT

Sequences:

>Translated_270_residues
MHRSSYKYWLKRKEHIDADFTILCSEVKAAHRISHGSAGARTIAQLVTNKGICLSRYRARNLMKKLGLLSCQQPKHSYRK
AAQEHVAIPNTLNRQFAVTQPDQVWCGDVTYVWVGNRWAYLAVVLDLFSRKPVGWALSLSPDSELTCKALKMAFELRGKP
ENVIYHSDQGSHYTSLKFRQLIWRLQIEQSMSRRGNCWDNAPMERFFRSLKSEWIPASGYGNFTEADKEITNYITGYYSE
TRPHQYNGGLTPNESERLYWNDSKTVANFT
>Mature_270_residues
MHRSSYKYWLKRKEHIDADFTILCSEVKAAHRISHGSAGARTIAQLVTNKGICLSRYRARNLMKKLGLLSCQQPKHSYRK
AAQEHVAIPNTLNRQFAVTQPDQVWCGDVTYVWVGNRWAYLAVVLDLFSRKPVGWALSLSPDSELTCKALKMAFELRGKP
ENVIYHSDQGSHYTSLKFRQLIWRLQIEQSMSRRGNCWDNAPMERFFRSLKSEWIPASGYGNFTEADKEITNYITGYYSE
TRPHQYNGGLTPNESERLYWNDSKTVANFT

Specific function: Involved in the transposition of the insertion sequence IS911B [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=194, Percent_Identity=31.4432989690722, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1788405, Length=167, Percent_Identity=40.1197604790419, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787263, Length=167, Percent_Identity=40.1197604790419, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786753, Length=167, Percent_Identity=40.1197604790419, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786570, Length=167, Percent_Identity=40.1197604790419, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786490, Length=167, Percent_Identity=40.1197604790419, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789981, Length=256, Percent_Identity=33.984375, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31226; Mature: 31226

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRSSYKYWLKRKEHIDADFTILCSEVKAAHRISHGSAGARTIAQLVTNKGICLSRYRAR
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHH
NLMKKLGLLSCQQPKHSYRKAAQEHVAIPNTLNRQFAVTQPDQVWCGDVTYVWVGNRWAY
HHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEECCEEEEEECCCHHH
LAVVLDLFSRKPVGWALSLSPDSELTCKALKMAFELRGKPENVIYHSDQGSHYTSLKFRQ
HHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCEEHHHHHHH
LIWRLQIEQSMSRRGNCWDNAPMERFFRSLKSEWIPASGYGNFTEADKEITNYITGYYSE
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
TRPHQYNGGLTPNESERLYWNDSKTVANFT
CCCCCCCCCCCCCCCCEEEECCCCEEECCC
>Mature Secondary Structure
MHRSSYKYWLKRKEHIDADFTILCSEVKAAHRISHGSAGARTIAQLVTNKGICLSRYRAR
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHH
NLMKKLGLLSCQQPKHSYRKAAQEHVAIPNTLNRQFAVTQPDQVWCGDVTYVWVGNRWAY
HHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCEEECCEEEEEECCCHHH
LAVVLDLFSRKPVGWALSLSPDSELTCKALKMAFELRGKPENVIYHSDQGSHYTSLKFRQ
HHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCEEHHHHHHH
LIWRLQIEQSMSRRGNCWDNAPMERFFRSLKSEWIPASGYGNFTEADKEITNYITGYYSE
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
TRPHQYNGGLTPNESERLYWNDSKTVANFT
CCCCCCCCCCCCCCCCEEEECCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]