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The map label for this gene is rpoH [H]

Identifier: 160877394

GI number: 160877394

Start: 5072924

End: 5073781

Strand: Reverse

Name: rpoH [H]

Synonym: Sbal195_4292

Alternate gene names: 160877394

Gene position: 5073781-5072924 (Counterclockwise)

Preceding gene: 160877395

Following gene: 160877388

Centisome position: 94.89

GC content: 45.8

Gene sequence:

>858_bases
ATGACCTTTCAAACGCAATCAATGGCACTGACAGTCCCACAGGGCGCCAGTAGTCTAGAAGCTTATATCCATTCAGTGAC
TAGCATCAGCATGCTGGACGCTGAGGCTGAGTATGAGTTGGCCAAGCGTTTACAGGAAACTGGTGATCTACAAGCTGCGA
AGCAACTGATTATGTCGCACTTACGATTTGTAGTGCACGTTGCAAAGGGCTATTCAGGCTATGGTTTGCCACAGGCAGAC
CTGATCCAAGAAGGCAACATAGGCTTGATGAAAGCGGTTAAACGCTTCGATCCCGATGTTGGCGTGCGTCTAGTGTCTTT
TGCTGTGCACTGGATCAAAGCTGAAATTCATGAATATGTGCTGAAAAACTGGCGCATTGTTAAAGTCGCGACCACTAAAG
CGCAACGTAAGTTGTTCTTTAACATCCGTAAAGCGAAAAAACGCTTAGGTTGGTTCAGCGATGCAGAAGTCACTATGGTG
GCTGAAAATTTAGGTGTGTCTAAGGCCGATGTGACCGAAATGGAATCACGTATGGCTGCGCAAGATCCGGCATTTGATTT
AACCAATGATCAAGACGAGGAGCATGATTTTGCCCCAGTCCATTATTTGGAAGATCATTCATCGGATTTAGCGGAAAACA
TTGAAAACGACAACTGGGAATCGAATGCCCAAGGTCGTTTATTATCGGCCATCAAAACCTTAGACGAACGCAGCCAGCAT
ATTCTGCATGCCCGTTGGTTAGATGACGATAAAACCACACTGCAAGAACTTGCAGAGACTTATCAAGTGTCGGCTGAACG
TATTCGCCAACTTGAGAAAAATGCCATGAACAAGCTGAAAGCTTGCATGGAAGCCTAA

Upstream 100 bases:

>100_bases
TCTGTCAATTAGCCGATAGTTCGTGAGCTTATTCTTAGGGTCTTTTGTTATTGAACCTTAGAATGCACTCGTAAGTAGTC
TGATAAATGTAGGAGAGCGA

Downstream 100 bases:

>100_bases
GTCGCATCACGTTTAAGGTGACAGTGATTTAGCCATAAAAAAACCTGCTACGGCAGGTTTTTTTATGGGTGAAATTTGGC
TTGATGACGTTGATAATAAA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTFQTQSMALTVPQGASSLEAYIHSVTSISMLDAEAEYELAKRLQETGDLQAAKQLIMSHLRFVVHVAKGYSGYGLPQAD
LIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYVLKNWRIVKVATTKAQRKLFFNIRKAKKRLGWFSDAEVTMV
AENLGVSKADVTEMESRMAAQDPAFDLTNDQDEEHDFAPVHYLEDHSSDLAENIENDNWESNAQGRLLSAIKTLDERSQH
ILHARWLDDDKTTLQELAETYQVSAERIRQLEKNAMNKLKACMEA

Sequences:

>Translated_285_residues
MTFQTQSMALTVPQGASSLEAYIHSVTSISMLDAEAEYELAKRLQETGDLQAAKQLIMSHLRFVVHVAKGYSGYGLPQAD
LIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYVLKNWRIVKVATTKAQRKLFFNIRKAKKRLGWFSDAEVTMV
AENLGVSKADVTEMESRMAAQDPAFDLTNDQDEEHDFAPVHYLEDHSSDLAENIENDNWESNAQGRLLSAIKTLDERSQH
ILHARWLDDDKTTLQELAETYQVSAERIRQLEKNAMNKLKACMEA
>Mature_284_residues
TFQTQSMALTVPQGASSLEAYIHSVTSISMLDAEAEYELAKRLQETGDLQAAKQLIMSHLRFVVHVAKGYSGYGLPQADL
IQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYVLKNWRIVKVATTKAQRKLFFNIRKAKKRLGWFSDAEVTMVA
ENLGVSKADVTEMESRMAAQDPAFDLTNDQDEEHDFAPVHYLEDHSSDLAENIENDNWESNAQGRLLSAIKTLDERSQHI
LHARWLDDDKTTLQELAETYQVSAERIRQLEKNAMNKLKACMEA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=287, Percent_Identity=66.5505226480836, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1789098, Length=283, Percent_Identity=26.1484098939929, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=27.7777777777778, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788231, Length=212, Percent_Identity=28.7735849056604, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32309; Mature: 32178

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFQTQSMALTVPQGASSLEAYIHSVTSISMLDAEAEYELAKRLQETGDLQAAKQLIMSH
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHH
LRFVVHVAKGYSGYGLPQADLIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYV
HHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LKNWRIVKVATTKAQRKLFFNIRKAKKRLGWFSDAEVTMVAENLGVSKADVTEMESRMAA
HHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHCCCCHHHHHHHHHHHHH
QDPAFDLTNDQDEEHDFAPVHYLEDHSSDLAENIENDNWESNAQGRLLSAIKTLDERSQH
CCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
ILHARWLDDDKTTLQELAETYQVSAERIRQLEKNAMNKLKACMEA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TFQTQSMALTVPQGASSLEAYIHSVTSISMLDAEAEYELAKRLQETGDLQAAKQLIMSH
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHH
LRFVVHVAKGYSGYGLPQADLIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYV
HHHHHHHHCCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LKNWRIVKVATTKAQRKLFFNIRKAKKRLGWFSDAEVTMVAENLGVSKADVTEMESRMAA
HHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHHHCCCCHHHHHHHHHHHHH
QDPAFDLTNDQDEEHDFAPVHYLEDHSSDLAENIENDNWESNAQGRLLSAIKTLDERSQH
CCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
ILHARWLDDDKTTLQELAETYQVSAERIRQLEKNAMNKLKACMEA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]