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The map label for this gene is menE [H]

Identifier: 160877393

GI number: 160877393

Start: 5071396

End: 5072823

Strand: Direct

Name: menE [H]

Synonym: Sbal195_4291

Alternate gene names: 160877393

Gene position: 5071396-5072823 (Clockwise)

Preceding gene: 160877392

Following gene: 160877398

Centisome position: 94.84

GC content: 50.49

Gene sequence:

>1428_bases
GTGAGGAAAACTAGTTTGTCTGACACCCGCCTATCGCAGTCGCTAGCACTGTCCCCCGTGCACCAAGCCGCGCTGGCGTT
TCCACAGCAGACGGCCAGCAAGTTGGCGGGACAAGACATTCGTTACTCTGAGCTTAGCCAGCGCGTTATCGCTCTGGGTC
AGCAGCTAAGAGCAGCAGGCATTGCAGAGGGTGAGCCACTCGCCTGTATTGCTGTGAATAACCTCGAGATGATTTGCCTC
TATTGGGCCTGCATCGATATCGGGGCGATCTTTTTCCCTATCTCACCCCGTTTTCCTCTCGCGCAGATCCAAGGTTTAAT
CGATGAACATCAGATCCGCTATGTTTGGCGTGCTGAGATTTCTGATCTGCAGAATTGCAGCCAATTAGCCTTAGATTTTA
ATCGCCTCAGCAACGAGTTAGCCCAGCCTATCGATGTTACCCGACCCGCCAATGTGATTTTAACGTCGGGGTCTAGCGGT
TTTCCAAAAGCGGCGGTCCATTGCTTAGCCAACCATATTGCTAATGCCGAAGGCGCTCGCAGCTTAATCGCGCTCGAACA
AGGTGATGCTTGGTTGCTCTCCTTGCCCTTATTCCACATCGGCGGTCTAGCGATATTAAACCGCTGTGCCTTAGTCGGTG
CCACTGTTGTTATGCCAGATCACGCGCTGAGTCTTAGTCAGCAAATTGACCAAGATAAGCTAACCCACCTCTCTTTGGTG
CCAGCACAACTGAGCAAGTTATTAGCCGACACAAGCAGCAAACTTATAACCATCAAAGCCTTACTACTGGGCGGCGGCGC
AGTCTCATTAGACTTACTCGCCGAGCTTAAGCTACGCAATATCGCCAGCTACACGAGCTACGGCATGACAGAAATGGGCT
CGCAGATCACCACAGGCCGCGCATTAAGCGACGGCAGCAGTGGCAAATTACTCCCCAAGCGTGAGCTTAAAATCGAAGAC
AGTGTGATTTGGGTGCGCGGCGAATGCCTGTTTATGGGCTATCTCACCCCTGAGGGAATACAAGCTGGCGTTGATCAAGA
AGGCTGGTTTTATACTAAAGACAGGGGCGAATGGGACGAAAATGGCAATCTACACATCCTTGGTCGCGTCGATAACATGT
TCATTTGTGGCGGCGAAAATATCCAACCAGAAGAAATCGAAGCCGCGCTTAAACAGCACCCACAAATAGATGATGCTATC
GTTTTCGCCATCCCCGATGCACAATTTGGCAATCTCCCCGCCGCTATCCTTCGCGGCAGTTTAACTCATCACACAGAGTC
GATTGCCAATGAACTCGAATTGTTCCTCGCCGAAAAAATCGCCCGCTTCAAACGCCCAAGACAATATTATGTATGGCCAG
AAAATCATGAGCAGGCAGGGCTGAAAGTAAATAGAAAAGCCTTGATAGCTAATCTTGTTAAACACTGA

Upstream 100 bases:

>100_bases
TCATCGGGTAAAAAACACTGCCTCACACTCGCCCAACTTGAACTCATTGCACAACACGCCATTGATTCAACGCAGCAAAG
TGATTTGGGTGCAGCCAAGC

Downstream 100 bases:

>100_bases
TTTATTATCAACGTCATCAAGCCAAATTTCACCCATAAAAAAACCTGCCGTAGCAGGTTTTTTTATGGCTAAATCACTGT
CACCTTAAACGTGATGCGAC

Product: O-succinylbenzoate-CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MRKTSLSDTRLSQSLALSPVHQAALAFPQQTASKLAGQDIRYSELSQRVIALGQQLRAAGIAEGEPLACIAVNNLEMICL
YWACIDIGAIFFPISPRFPLAQIQGLIDEHQIRYVWRAEISDLQNCSQLALDFNRLSNELAQPIDVTRPANVILTSGSSG
FPKAAVHCLANHIANAEGARSLIALEQGDAWLLSLPLFHIGGLAILNRCALVGATVVMPDHALSLSQQIDQDKLTHLSLV
PAQLSKLLADTSSKLITIKALLLGGGAVSLDLLAELKLRNIASYTSYGMTEMGSQITTGRALSDGSSGKLLPKRELKIED
SVIWVRGECLFMGYLTPEGIQAGVDQEGWFYTKDRGEWDENGNLHILGRVDNMFICGGENIQPEEIEAALKQHPQIDDAI
VFAIPDAQFGNLPAAILRGSLTHHTESIANELELFLAEKIARFKRPRQYYVWPENHEQAGLKVNRKALIANLVKH

Sequences:

>Translated_475_residues
MRKTSLSDTRLSQSLALSPVHQAALAFPQQTASKLAGQDIRYSELSQRVIALGQQLRAAGIAEGEPLACIAVNNLEMICL
YWACIDIGAIFFPISPRFPLAQIQGLIDEHQIRYVWRAEISDLQNCSQLALDFNRLSNELAQPIDVTRPANVILTSGSSG
FPKAAVHCLANHIANAEGARSLIALEQGDAWLLSLPLFHIGGLAILNRCALVGATVVMPDHALSLSQQIDQDKLTHLSLV
PAQLSKLLADTSSKLITIKALLLGGGAVSLDLLAELKLRNIASYTSYGMTEMGSQITTGRALSDGSSGKLLPKRELKIED
SVIWVRGECLFMGYLTPEGIQAGVDQEGWFYTKDRGEWDENGNLHILGRVDNMFICGGENIQPEEIEAALKQHPQIDDAI
VFAIPDAQFGNLPAAILRGSLTHHTESIANELELFLAEKIARFKRPRQYYVWPENHEQAGLKVNRKALIANLVKH
>Mature_475_residues
MRKTSLSDTRLSQSLALSPVHQAALAFPQQTASKLAGQDIRYSELSQRVIALGQQLRAAGIAEGEPLACIAVNNLEMICL
YWACIDIGAIFFPISPRFPLAQIQGLIDEHQIRYVWRAEISDLQNCSQLALDFNRLSNELAQPIDVTRPANVILTSGSSG
FPKAAVHCLANHIANAEGARSLIALEQGDAWLLSLPLFHIGGLAILNRCALVGATVVMPDHALSLSQQIDQDKLTHLSLV
PAQLSKLLADTSSKLITIKALLLGGGAVSLDLLAELKLRNIASYTSYGMTEMGSQITTGRALSDGSSGKLLPKRELKIED
SVIWVRGECLFMGYLTPEGIQAGVDQEGWFYTKDRGEWDENGNLHILGRVDNMFICGGENIQPEEIEAALKQHPQIDDAI
VFAIPDAQFGNLPAAILRGSLTHHTESIANELELFLAEKIARFKRPRQYYVWPENHEQAGLKVNRKALIANLVKH

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=340, Percent_Identity=26.4705882352941, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI115511026, Length=497, Percent_Identity=24.3460764587525, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI38505220, Length=381, Percent_Identity=23.0971128608924, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI122937307, Length=330, Percent_Identity=25.1515151515152, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI157311624, Length=353, Percent_Identity=25.2124645892351, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI157311622, Length=353, Percent_Identity=25.2124645892351, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI58082049, Length=460, Percent_Identity=23.0434782608696, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI27477105, Length=137, Percent_Identity=35.036496350365, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788595, Length=441, Percent_Identity=33.7868480725624, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI145693145, Length=490, Percent_Identity=25.9183673469388, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI221142682, Length=488, Percent_Identity=23.155737704918, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788107, Length=283, Percent_Identity=25.4416961130742, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32563687, Length=489, Percent_Identity=26.1758691206544, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17558820, Length=510, Percent_Identity=26.078431372549, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=348, Percent_Identity=30.4597701149425, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=379, Percent_Identity=28.7598944591029, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560308, Length=328, Percent_Identity=25.9146341463415, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17538037, Length=383, Percent_Identity=27.4151436031332, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71994694, Length=336, Percent_Identity=26.1904761904762, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71994690, Length=336, Percent_Identity=26.1904761904762, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71994703, Length=336, Percent_Identity=26.1904761904762, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=341, Percent_Identity=25.8064516129032, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17531443, Length=490, Percent_Identity=21.6326530612245, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71996755, Length=446, Percent_Identity=22.1973094170404, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560140, Length=282, Percent_Identity=25.886524822695, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=345, Percent_Identity=25.2173913043478, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24581924, Length=335, Percent_Identity=31.9402985074627, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI19922652, Length=438, Percent_Identity=24.2009132420091, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648260, Length=441, Percent_Identity=23.8095238095238, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI18859661, Length=359, Percent_Identity=26.4623955431755, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356947, Length=289, Percent_Identity=26.643598615917, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24653035, Length=418, Percent_Identity=24.8803827751196, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI161076582, Length=422, Percent_Identity=24.6445497630332, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356441, Length=412, Percent_Identity=21.1165048543689, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20130357, Length=287, Percent_Identity=27.8745644599303, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21358303, Length=427, Percent_Identity=20.8430913348946, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21355181, Length=472, Percent_Identity=21.8220338983051, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648257, Length=486, Percent_Identity=21.8106995884774, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19921316, Length=143, Percent_Identity=34.2657342657343, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 51988; Mature: 51988

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTSLSDTRLSQSLALSPVHQAALAFPQQTASKLAGQDIRYSELSQRVIALGQQLRAAG
CCCCCCCHHHHHHHHCCCHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
IAEGEPLACIAVNNLEMICLYWACIDIGAIFFPISPRFPLAQIQGLIDEHQIRYVWRAEI
CCCCCCEEEEEECCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHEEEEEEHHH
SDLQNCSQLALDFNRLSNELAQPIDVTRPANVILTSGSSGFPKAAVHCLANHIANAEGAR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCC
SLIALEQGDAWLLSLPLFHIGGLAILNRCALVGATVVMPDHALSLSQQIDQDKLTHLSLV
EEEEEECCCEEEEECHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHCCHHHHHHHEEC
PAQLSKLLADTSSKLITIKALLLGGGAVSLDLLAELKLRNIASYTSYGMTEMGSQITTGR
HHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCC
ALSDGSSGKLLPKRELKIEDSVIWVRGECLFMGYLTPEGIQAGVDQEGWFYTKDRGEWDE
EECCCCCCCCCCCCCCEEECCEEEEECCEEEEECCCCCHHHCCCCCCCCEEECCCCCCCC
NGNLHILGRVDNMFICGGENIQPEEIEAALKQHPQIDDAIVFAIPDAQFGNLPAAILRGS
CCCEEEEEECCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHCC
LTHHTESIANELELFLAEKIARFKRPRQYYVWPENHEQAGLKVNRKALIANLVKH
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEHHHHHHHHHHCC
>Mature Secondary Structure
MRKTSLSDTRLSQSLALSPVHQAALAFPQQTASKLAGQDIRYSELSQRVIALGQQLRAAG
CCCCCCCHHHHHHHHCCCHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
IAEGEPLACIAVNNLEMICLYWACIDIGAIFFPISPRFPLAQIQGLIDEHQIRYVWRAEI
CCCCCCEEEEEECCCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHEEEEEEHHH
SDLQNCSQLALDFNRLSNELAQPIDVTRPANVILTSGSSGFPKAAVHCLANHIANAEGAR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCC
SLIALEQGDAWLLSLPLFHIGGLAILNRCALVGATVVMPDHALSLSQQIDQDKLTHLSLV
EEEEEECCCEEEEECHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHCCHHHHHHHEEC
PAQLSKLLADTSSKLITIKALLLGGGAVSLDLLAELKLRNIASYTSYGMTEMGSQITTGR
HHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCC
ALSDGSSGKLLPKRELKIEDSVIWVRGECLFMGYLTPEGIQAGVDQEGWFYTKDRGEWDE
EECCCCCCCCCCCCCCEEECCEEEEECCEEEEECCCCCHHHCCCCCCCCEEECCCCCCCC
NGNLHILGRVDNMFICGGENIQPEEIEAALKQHPQIDDAIVFAIPDAQFGNLPAAILRGS
CCCEEEEEECCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHCC
LTHHTESIANELELFLAEKIARFKRPRQYYVWPENHEQAGLKVNRKALIANLVKH
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]