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The map label for this gene is fdhB1 [H]

Identifier: 160877331

GI number: 160877331

Start: 5005822

End: 5006391

Strand: Direct

Name: fdhB1 [H]

Synonym: Sbal195_4229

Alternate gene names: 160877331

Gene position: 5005822-5006391 (Clockwise)

Preceding gene: 160877330

Following gene: 160877332

Centisome position: 93.61

GC content: 50.35

Gene sequence:

>570_bases
ATGGCTACAATGAAATTTTTGTGTGATACCAAGCGCTGCATCGAATGTAACGGTTGTGTCACTGCCTGTAAAAACGAAAA
CGACTCCGCTCTCGAGTGGGGGATCCAACGCCGCCGCGTAGTGACCATTAACGATGGTCAACGTGGAGAAGCCTCTATCT
CTGTCGCCTGTATGCATTGCTCAGATGCACCTTGTATGGCAGTTTGCCCAGCAAATTGCTTCTACAGAACCGACGACGGC
ATAGTGCTACACAACAAAGATGCCTGTATTGGTTGCGGTTACTGCTTGTACGCCTGTCCGTTTGGCGCACCACAGTTCCC
GAAAAAAGGTGCCTTTGGTAGCCGTGGCAAGATGGATAAATGTACCTTCTGCGCTGGCGGCCCAGAAGAAAACCACTCAG
AGGCAGAGCGCCAAAAATACGGTTCAAACCGTATTGCCGAAGGCAAACTGCCTATGTGTGCAGAACTGTGTGCCACTAAG
GCCCTGTTAGCGGGTGATGCTGAAATTGTTTCAAACATCTTCCGTGAACGTGTGGCTTACCGTGGCGCTAAAAACGCGGC
TTGGAATTAA

Upstream 100 bases:

>100_bases
CCTATGGTTATGATCCTGTGACGCAAATGCAGGAAACCAAGGCATCACTGTGTCAGATCGTGAAAGCGTAAGTGCAGCTT
AAGCTAGGAGAATTGCCATT

Downstream 100 bases:

>100_bases
TTCAGGTCAACAGGATCTGTCCACAGACTTAGCTTGGCGCCTGCAGGCAGGCGCCACTAAGCGAGAGAGGATGTATGATG
AACAAATGGTTCAAACACAT

Product: 4Fe-4S ferredoxin iron-sulfur-binding domain-containing protein

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGQRGEASISVACMHCSDAPCMAVCPANCFYRTDDG
IVLHNKDACIGCGYCLYACPFGAPQFPKKGAFGSRGKMDKCTFCAGGPEENHSEAERQKYGSNRIAEGKLPMCAELCATK
ALLAGDAEIVSNIFRERVAYRGAKNAAWN

Sequences:

>Translated_189_residues
MATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGQRGEASISVACMHCSDAPCMAVCPANCFYRTDDG
IVLHNKDACIGCGYCLYACPFGAPQFPKKGAFGSRGKMDKCTFCAGGPEENHSEAERQKYGSNRIAEGKLPMCAELCATK
ALLAGDAEIVSNIFRERVAYRGAKNAAWN
>Mature_188_residues
ATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGQRGEASISVACMHCSDAPCMAVCPANCFYRTDDGI
VLHNKDACIGCGYCLYACPFGAPQFPKKGAFGSRGKMDKCTFCAGGPEENHSEAERQKYGSNRIAEGKLPMCAELCATKA
LLAGDAEIVSNIFRERVAYRGAKNAAWN

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787122, Length=169, Percent_Identity=33.7278106508876, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1787872, Length=172, Percent_Identity=33.7209302325581, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1789370, Length=202, Percent_Identity=28.2178217821782, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1790326, Length=203, Percent_Identity=31.5270935960591, Blast_Score=80, Evalue=7e-17,
Organism=Escherichia coli, GI226510944, Length=161, Percent_Identity=32.9192546583851, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI2367345, Length=184, Percent_Identity=30.9782608695652, Blast_Score=77, Evalue=5e-16,
Organism=Escherichia coli, GI1787749, Length=202, Percent_Identity=28.2178217821782, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1788811, Length=126, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87082114, Length=139, Percent_Identity=32.3741007194245, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789079, Length=101, Percent_Identity=36.6336633663366, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI87082180, Length=103, Percent_Identity=36.8932038834951, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI2367245, Length=172, Percent_Identity=31.3953488372093, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 20534; Mature: 20402

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
12.7 %Cys+Met (Translated Protein)
9.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
12.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGQRGEASISVACMHC
CCCHHHHHCCCCEEECCCCEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEC
SDAPCMAVCPANCFYRTDDGIVLHNKDACIGCGYCLYACPFGAPQFPKKGAFGSRGKMDK
CCCCEEEECCCCCEEECCCCEEEECCCCEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCC
CTFCAGGPEENHSEAERQKYGSNRIAEGKLPMCAELCATKALLAGDAEIVSNIFRERVAY
EEEECCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RGAKNAAWN
CCCCCCCCC
>Mature Secondary Structure 
ATMKFLCDTKRCIECNGCVTACKNENDSALEWGIQRRRVVTINDGQRGEASISVACMHC
CCHHHHHCCCCEEECCCCEEECCCCCCHHHHHHHCCCEEEEECCCCCCCCEEEEEEEEC
SDAPCMAVCPANCFYRTDDGIVLHNKDACIGCGYCLYACPFGAPQFPKKGAFGSRGKMDK
CCCCEEEECCCCCEEECCCCEEEECCCCEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCC
CTFCAGGPEENHSEAERQKYGSNRIAEGKLPMCAELCATKALLAGDAEIVSNIFRERVAY
EEEECCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RGAKNAAWN
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]