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The map label for this gene is dusB [H]

Identifier: 160877184

GI number: 160877184

Start: 4812527

End: 4813495

Strand: Direct

Name: dusB [H]

Synonym: Sbal195_4080

Alternate gene names: 160877184

Gene position: 4812527-4813495 (Clockwise)

Preceding gene: 160877183

Following gene: 160877185

Centisome position: 90.0

GC content: 50.05

Gene sequence:

>969_bases
ATGCAGATTGGACCCTATCAACTGAACAATCAGCTGATCGTGGCACCAATGGCAGGTGTCACCGATCAAGCTTTCCGTAA
TCTTTGTCTTCGTTATGGCGCAGCCCTAGCGGTTTCCGAGATGCTTTCATCCAATCCTGAGGTTTGGGATACAGATAAAA
GCCGCCAGCGCATGACGCATTCTGGCGAGGAAGGCATCCGCTCAGTGCAAATTGCAGGAGCCGATCCTGATTTAATGGCG
CAAGCCGCGTTGTTCAATGTTGAACAAGGCGCTCACATCATTGATATCAATATGGGTTGCCCCGCGAAAAAAGTGAATAA
AAAGCTGGCTGGCTCAGCGCTGATGCAAAACCCGCCGCTGGTAAAAGAAATTTTACAAGCCGTGGTCGCCGCAGTGAATG
TGCCCGTCACGTTAAAGATTCGCACCGGCTGGGAGCCAGAACACAAGAATGGTGTTCAAATCGCCCAGATTGCCCAAGAT
TGTGGCATCGCCTCGCTCGCCGTTCACGGTCGCACGAGACAATGTATGTACAGAGGCGACGCTGAGTACGACACGATTAA
AGCAATCAAACAGAATGTCTCGATTCCTGTCGTCGCTAATGGGGATATCGACAGTCCGGAGAAAGCCCGCTTTGTGCTGG
AATACACCGGTGCCGATGCCTTGATGATAGGACGGGGTGCTCAAGGACGGCCTTGGATTTTCAGAGAAATCCAGCACTAC
TTGGAAACGGGTAATAAGCTAGCGCCCATTGAGGTTGCCGAACAGCGTCAAGTGATGCTGGAACACCTCACCAAACTGTA
TGATTTGTATGGCGAATACAAGGGTATCCGCTTCGCCAGAAAGCATATGGGATGGTACCTAGACCAAGAAGCTCAGCGCC
AATTTAGGGCTGACTTTAATCGGCTGGAAACCGCTGCAGAACAGTATTCCCTCGTGGAATGTTATTTTGATGAATTAGTA
CAGAATTAA

Upstream 100 bases:

>100_bases
TCAAGAAAAAAAATTACATCTAGGTCATTTATTGACCTTTTCACGGCCAACAAAAAGGGCTACTATAGCGCCCCTTTGAA
GAGCTAAGGTGAAACATAAA

Downstream 100 bases:

>100_bases
TAAAGAGCAGAATAGAATGTTTGATCAGACAACTAACACAGAAGTTCACCAGCTTACCGTTGGCAAAATCGAAACCGCAA
ACGGCACTATCAAGCCCCAG

Product: NifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MQIGPYQLNNQLIVAPMAGVTDQAFRNLCLRYGAALAVSEMLSSNPEVWDTDKSRQRMTHSGEEGIRSVQIAGADPDLMA
QAALFNVEQGAHIIDINMGCPAKKVNKKLAGSALMQNPPLVKEILQAVVAAVNVPVTLKIRTGWEPEHKNGVQIAQIAQD
CGIASLAVHGRTRQCMYRGDAEYDTIKAIKQNVSIPVVANGDIDSPEKARFVLEYTGADALMIGRGAQGRPWIFREIQHY
LETGNKLAPIEVAEQRQVMLEHLTKLYDLYGEYKGIRFARKHMGWYLDQEAQRQFRADFNRLETAAEQYSLVECYFDELV
QN

Sequences:

>Translated_322_residues
MQIGPYQLNNQLIVAPMAGVTDQAFRNLCLRYGAALAVSEMLSSNPEVWDTDKSRQRMTHSGEEGIRSVQIAGADPDLMA
QAALFNVEQGAHIIDINMGCPAKKVNKKLAGSALMQNPPLVKEILQAVVAAVNVPVTLKIRTGWEPEHKNGVQIAQIAQD
CGIASLAVHGRTRQCMYRGDAEYDTIKAIKQNVSIPVVANGDIDSPEKARFVLEYTGADALMIGRGAQGRPWIFREIQHY
LETGNKLAPIEVAEQRQVMLEHLTKLYDLYGEYKGIRFARKHMGWYLDQEAQRQFRADFNRLETAAEQYSLVECYFDELV
QN
>Mature_322_residues
MQIGPYQLNNQLIVAPMAGVTDQAFRNLCLRYGAALAVSEMLSSNPEVWDTDKSRQRMTHSGEEGIRSVQIAGADPDLMA
QAALFNVEQGAHIIDINMGCPAKKVNKKLAGSALMQNPPLVKEILQAVVAAVNVPVTLKIRTGWEPEHKNGVQIAQIAQD
CGIASLAVHGRTRQCMYRGDAEYDTIKAIKQNVSIPVVANGDIDSPEKARFVLEYTGADALMIGRGAQGRPWIFREIQHY
LETGNKLAPIEVAEQRQVMLEHLTKLYDLYGEYKGIRFARKHMGWYLDQEAQRQFRADFNRLETAAEQYSLVECYFDELV
QN

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=239, Percent_Identity=33.4728033472803, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI239788462, Length=240, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=34.2222222222222, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI40807366, Length=299, Percent_Identity=28.4280936454849, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI8923374, Length=252, Percent_Identity=32.1428571428571, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=64.375, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1788462, Length=239, Percent_Identity=31.7991631799163, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI145693211, Length=328, Percent_Identity=26.5243902439024, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=242, Percent_Identity=33.0578512396694, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=230, Percent_Identity=30, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17510279, Length=274, Percent_Identity=29.5620437956204, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=243, Percent_Identity=29.6296296296296, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=33.7662337662338, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323433, Length=312, Percent_Identity=26.9230769230769, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=26.9911504424779, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24580595, Length=227, Percent_Identity=32.5991189427313, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19920448, Length=227, Percent_Identity=32.5991189427313, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24585320, Length=290, Percent_Identity=27.2413793103448, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI45549423, Length=244, Percent_Identity=29.5081967213115, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921524, Length=297, Percent_Identity=24.9158249158249, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35979; Mature: 35979

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGPYQLNNQLIVAPMAGVTDQAFRNLCLRYGAALAVSEMLSSNPEVWDTDKSRQRMTH
CCCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
SGEEGIRSVQIAGADPDLMAQAALFNVEQGAHIIDINMGCPAKKVNKKLAGSALMQNPPL
CCHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCHH
VKEILQAVVAAVNVPVTLKIRTGWEPEHKNGVQIAQIAQDCGIASLAVHGRTRQCMYRGD
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCEEEEEECCCCHHHHCCCC
AEYDTIKAIKQNVSIPVVANGDIDSPEKARFVLEYTGADALMIGRGAQGRPWIFREIQHY
CCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LETGNKLAPIEVAEQRQVMLEHLTKLYDLYGEYKGIRFARKHMGWYLDQEAQRQFRADFN
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RLETAAEQYSLVECYFDELVQN
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQIGPYQLNNQLIVAPMAGVTDQAFRNLCLRYGAALAVSEMLSSNPEVWDTDKSRQRMTH
CCCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHC
SGEEGIRSVQIAGADPDLMAQAALFNVEQGAHIIDINMGCPAKKVNKKLAGSALMQNPPL
CCHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCHH
VKEILQAVVAAVNVPVTLKIRTGWEPEHKNGVQIAQIAQDCGIASLAVHGRTRQCMYRGD
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCEEEEEECCCCHHHHCCCC
AEYDTIKAIKQNVSIPVVANGDIDSPEKARFVLEYTGADALMIGRGAQGRPWIFREIQHY
CCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LETGNKLAPIEVAEQRQVMLEHLTKLYDLYGEYKGIRFARKHMGWYLDQEAQRQFRADFN
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RLETAAEQYSLVECYFDELVQN
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]