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The map label for this gene is tapD [H]

Identifier: 160877166

GI number: 160877166

Start: 4795799

End: 4796713

Strand: Direct

Name: tapD [H]

Synonym: Sbal195_4062

Alternate gene names: 160877166

Gene position: 4795799-4796713 (Clockwise)

Preceding gene: 160877165

Following gene: 160877167

Centisome position: 89.69

GC content: 45.46

Gene sequence:

>915_bases
ATGTCAGAATTCATCTCCATTTTGGGCCAGCACCCTTATCTTTTTATGGCTCTAAGCTTTATTTTTGCCGCTACCATTGG
TAGTTTTTTAAATGTGGTGATCCACAGATTTCCTGTGATGATGAAACGGGAGTGGCAGCAAGAATGTAATCAGTATTTAC
AGGAATACCACGAAGACATAGTCAAAAAAATAGGGATAGACCAACTCAATAAACCTATAGATGCCTTCCCTGAAAAGTAT
AATCTTGTTGTGCCGGGCTCAGCCTGCCCTAAGTGCAAAACGTCGATTAAACCTTGGCATAATTTACCCGTATTAGGCTG
GTTAATGCTCAAGGGAAAATGCGCCGCCTGTCACGCCCCTATTTCTGCTCGCTACCCCATTATTGAATTCATCACAGGGC
TACTTATCGCGACTTTAGCTTGGCATTTTGGTCCAAGCTGGCAGTTTGTTTTTGCGAGCATACTCACCTTTGTGCTTGTA
GCACTGACAGGTATCGATCTTGATGAGATGTTGCTCCCCGACCAAATGACGCTACCGTTGCTTTGGCTCGGCTTGATAAT
CAATTTAAATCATATCTTTGCGAGCCCAACCGATGCCATTATCGGTGCGGCGGCAGGCTATTTGAGTCTATGGTCCATTT
TCTGGTTGTTTAAAATCCTCACCGGCAAGGAAGGCATGGGCTATGGCGACTTTAAATTACTCGCCGTGTTCGGTGCTTGG
CTTGGCTGGCAAATGCTGCCACTGGTGATTTTACTTTCGTCCCTCGTTGCCGCAGTCGTTGGCATCACTATGATTGTTAG
TAAACGCCTGAAACAAGGGAATCCAATTCCATTTGGCCCCTATATTGCTGCCGCAGGCTGGATAGCCTTGCTATTTGGAC
AGCAGATTGTCGATTGGTACCTATCAACACTGTAA

Upstream 100 bases:

>100_bases
GTGGTCTAATTGTTGCTATGTACTTGCCTATCTTCCAAATGGGTAAAATTGTTAGCGGCTAGTATTATATCTAACCCTCA
AATATAAATAAGAACATTAA

Downstream 100 bases:

>100_bases
AAGCATGCAAACTAAGCACAAAGCTATCCGGAGTATGTTATGTCAAAGTTTGTCGTTGGCCTTACAGGCGGTATTGGCAG
CGGTAAAACAACGGTTGCCA

Product: prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSEFISILGQHPYLFMALSFIFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHEDIVKKIGIDQLNKPIDAFPEKY
NLVVPGSACPKCKTSIKPWHNLPVLGWLMLKGKCAACHAPISARYPIIEFITGLLIATLAWHFGPSWQFVFASILTFVLV
ALTGIDLDEMLLPDQMTLPLLWLGLIINLNHIFASPTDAIIGAAAGYLSLWSIFWLFKILTGKEGMGYGDFKLLAVFGAW
LGWQMLPLVILLSSLVAAVVGITMIVSKRLKQGNPIPFGPYIAAAGWIALLFGQQIVDWYLSTL

Sequences:

>Translated_304_residues
MSEFISILGQHPYLFMALSFIFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHEDIVKKIGIDQLNKPIDAFPEKY
NLVVPGSACPKCKTSIKPWHNLPVLGWLMLKGKCAACHAPISARYPIIEFITGLLIATLAWHFGPSWQFVFASILTFVLV
ALTGIDLDEMLLPDQMTLPLLWLGLIINLNHIFASPTDAIIGAAAGYLSLWSIFWLFKILTGKEGMGYGDFKLLAVFGAW
LGWQMLPLVILLSSLVAAVVGITMIVSKRLKQGNPIPFGPYIAAAGWIALLFGQQIVDWYLSTL
>Mature_303_residues
SEFISILGQHPYLFMALSFIFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHEDIVKKIGIDQLNKPIDAFPEKYN
LVVPGSACPKCKTSIKPWHNLPVLGWLMLKGKCAACHAPISARYPIIEFITGLLIATLAWHFGPSWQFVFASILTFVLVA
LTGIDLDEMLLPDQMTLPLLWLGLIINLNHIFASPTDAIIGAAAGYLSLWSIFWLFKILTGKEGMGYGDFKLLAVFGAWL
GWQMLPLVILLSSLVAAVVGITMIVSKRLKQGNPIPFGPYIAAAGWIALLFGQQIVDWYLSTL

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=294, Percent_Identity=33.6734693877551, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1789732, Length=132, Percent_Identity=44.6969696969697, Blast_Score=88, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 33974; Mature: 33843

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFISILGQHPYLFMALSFIFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHEDI
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKKIGIDQLNKPIDAFPEKYNLVVPGSACPKCKTSIKPWHNLPVLGWLMLKGKCAACHAP
HHHHCHHHHCCCHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHCCC
ISARYPIIEFITGLLIATLAWHFGPSWQFVFASILTFVLVALTGIDLDEMLLPDQMTLPL
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHH
LWLGLIINLNHIFASPTDAIIGAAAGYLSLWSIFWLFKILTGKEGMGYGDFKLLAVFGAW
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LGWQMLPLVILLSSLVAAVVGITMIVSKRLKQGNPIPFGPYIAAAGWIALLFGQQIVDWY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSTL
HHHC
>Mature Secondary Structure 
SEFISILGQHPYLFMALSFIFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHEDI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA