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The map label for this gene is purD [H]

Identifier: 160877141

GI number: 160877141

Start: 4749382

End: 4750680

Strand: Direct

Name: purD [H]

Synonym: Sbal195_4037

Alternate gene names: 160877141

Gene position: 4749382-4750680 (Clockwise)

Preceding gene: 160877140

Following gene: 160877142

Centisome position: 88.82

GC content: 51.35

Gene sequence:

>1299_bases
ATGAAAGTATTAGTTATTGGTGGCGGCGGTCGCGAACATGCCCTAGCGTGGAAAGCTGCACAATCGGCTCAAGTTGAAAC
TGTCTATGTTGCGCCAGGCAATGCGGGCACAGCCCTAGAACCTAATGTTGAAAATCTGGCGATCAGCGCCACAGACATTC
CTGCTCTGCTCGATTTTGCGAAAACCAATAAGATTGAACTGACCATAGTCGGCCCAGAAGCGCCATTAGTGATGGGCGTA
GTTGATGCATTCAACGCCGCAGGTTTACCGATTTTTGGCCCAACTAAGGCGGCTGCTCAGCTCGAAGGTTCAAAAGCCTT
CACTAAGGACTTTTTAGCACGCCACAATATTCCAACAGCGGGTTACAAAAACTGCACTGAAATCAGCGATGCAAAAGCCT
TCGTGCGTGAATTAACGGCCACAACAGGTTACCCAGTGGTCATCAAAGCCGACGGTTTAGCCGCAGGTAAAGGCGTGATT
ATCGCCCAAGACCAAGCTGAAGCCGATGCTGCGATTGATGACATGCTCGCTGGTAACAAGTTTGGCGATGCGGGTTCACG
CGTGGTCATTGAAGAATTCCTGAAAGGCGAAGAAGCCAGCTTCATCGTAATGGTCGATGGCAAAAATATTCTGCCAATGG
CGACCAGCCAAGATCATAAAGCCCGCGATAACGGCGACTATGGTCCGAATACTGGCGGCATGGGTGCTTACTCGCCTGCT
CCTGTAGTGACTCAAGCGGTACACGATTGGACTGTGGCAAACGTGATTCGCCCAACGGTTGATGGCATGGCGGCAGAAGG
CAATGTCTACACAGGCTTTTTGTATGCTGGCTTGATGATATCACCCGATGGCAGTGCCAAAGTGCTCGAGTACAACTGCC
GCTTCGGCGATCCAGAAACTCAGCCAATCATGATGCGGTTAAAGTCAGACTTAGTGGAACTGTGTTTAGCCGCCACCCGT
GGCGAGTTAGATAAAGTGACAGCGGAATACGACGAGCGCGCAGCCGTGGGTGTAGTATTAGCCGCTGGCGGTTACCCAGA
TGAGTATCGCAAGTTTGATGTGATTGACGGTCTCAGCCTTGGTGATAACAATGGCAAAGTCTTCCATGCGGGCACTGAGA
TGAAAGACGGCCACGTAGTCACCAACGGCGGTCGCGTACTCTGCGCCACTGCACTGGGTAACACAGTGACCGAAGCGCAA
AAAGCCGCCTATAAATTGGTTGATGAAATCCACTGGGATGATGTGTATTTCCGTACCGATATTGGCTACCGCGCGATTGC
ACGTGAACAGCAAGACTAA

Upstream 100 bases:

>100_bases
ACTGGCTGCTTTTTTGTTTTACCACTCTTTGTTTTACCGCACTCTGGTTTCGACCACAGTGTGCCTACACAGAACATAAA
ACTCTAAGGGATGACTTAAC

Downstream 100 bases:

>100_bases
TTGCTAGGCGCGTTAAAAAACCGGCCAGATGCCGGTTTTTTTGTGCCTGAAATTTGACGAGCGTCAAATGTAACGTCATG
ATTTTCAATAAAATTACATA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKVLVIGGGGREHALAWKAAQSAQVETVYVAPGNAGTALEPNVENLAISATDIPALLDFAKTNKIELTIVGPEAPLVMGV
VDAFNAAGLPIFGPTKAAAQLEGSKAFTKDFLARHNIPTAGYKNCTEISDAKAFVRELTATTGYPVVIKADGLAAGKGVI
IAQDQAEADAAIDDMLAGNKFGDAGSRVVIEEFLKGEEASFIVMVDGKNILPMATSQDHKARDNGDYGPNTGGMGAYSPA
PVVTQAVHDWTVANVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPETQPIMMRLKSDLVELCLAATR
GELDKVTAEYDERAAVGVVLAAGGYPDEYRKFDVIDGLSLGDNNGKVFHAGTEMKDGHVVTNGGRVLCATALGNTVTEAQ
KAAYKLVDEIHWDDVYFRTDIGYRAIAREQQD

Sequences:

>Translated_432_residues
MKVLVIGGGGREHALAWKAAQSAQVETVYVAPGNAGTALEPNVENLAISATDIPALLDFAKTNKIELTIVGPEAPLVMGV
VDAFNAAGLPIFGPTKAAAQLEGSKAFTKDFLARHNIPTAGYKNCTEISDAKAFVRELTATTGYPVVIKADGLAAGKGVI
IAQDQAEADAAIDDMLAGNKFGDAGSRVVIEEFLKGEEASFIVMVDGKNILPMATSQDHKARDNGDYGPNTGGMGAYSPA
PVVTQAVHDWTVANVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPETQPIMMRLKSDLVELCLAATR
GELDKVTAEYDERAAVGVVLAAGGYPDEYRKFDVIDGLSLGDNNGKVFHAGTEMKDGHVVTNGGRVLCATALGNTVTEAQ
KAAYKLVDEIHWDDVYFRTDIGYRAIAREQQD
>Mature_432_residues
MKVLVIGGGGREHALAWKAAQSAQVETVYVAPGNAGTALEPNVENLAISATDIPALLDFAKTNKIELTIVGPEAPLVMGV
VDAFNAAGLPIFGPTKAAAQLEGSKAFTKDFLARHNIPTAGYKNCTEISDAKAFVRELTATTGYPVVIKADGLAAGKGVI
IAQDQAEADAAIDDMLAGNKFGDAGSRVVIEEFLKGEEASFIVMVDGKNILPMATSQDHKARDNGDYGPNTGGMGAYSPA
PVVTQAVHDWTVANVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPETQPIMMRLKSDLVELCLAATR
GELDKVTAEYDERAAVGVVLAAGGYPDEYRKFDVIDGLSLGDNNGKVFHAGTEMKDGHVVTNGGRVLCATALGNTVTEAQ
KAAYKLVDEIHWDDVYFRTDIGYRAIAREQQD

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=433, Percent_Identity=48.729792147806, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI4503915, Length=433, Percent_Identity=48.729792147806, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI209869995, Length=433, Percent_Identity=48.729792147806, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI209869993, Length=433, Percent_Identity=48.729792147806, Blast_Score=384, Evalue=1e-107,
Organism=Escherichia coli, GI1790438, Length=432, Percent_Identity=72.2222222222222, Blast_Score=620, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=41.0377358490566, Blast_Score=301, Evalue=5e-82,
Organism=Saccharomyces cerevisiae, GI6321203, Length=447, Percent_Identity=43.6241610738255, Blast_Score=334, Evalue=2e-92,
Organism=Drosophila melanogaster, GI62471619, Length=434, Percent_Identity=44.7004608294931, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24582400, Length=434, Percent_Identity=44.7004608294931, Blast_Score=336, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45708; Mature: 45708

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVIGGGGREHALAWKAAQSAQVETVYVAPGNAGTALEPNVENLAISATDIPALLDFA
CEEEEEECCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHH
KTNKIELTIVGPEAPLVMGVVDAFNAAGLPIFGPTKAAAQLEGSKAFTKDFLARHNIPTA
CCCEEEEEEECCCCCEEHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCC
GYKNCTEISDAKAFVRELTATTGYPVVIKADGLAAGKGVIIAQDQAEADAAIDDMLAGNK
CCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCC
FGDAGSRVVIEEFLKGEEASFIVMVDGKNILPMATSQDHKARDNGDYGPNTGGMGAYSPA
CCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
PVVTQAVHDWTVANVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPET
CHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCC
QPIMMRLKSDLVELCLAATRGELDKVTAEYDERAAVGVVLAAGGYPDEYRKFDVIDGLSL
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEEECCCCHHHCEEEEECCCEE
GDNNGKVFHAGTEMKDGHVVTNGGRVLCATALGNTVTEAQKAAYKLVDEIHWDDVYFRTD
CCCCCEEEECCCCCCCCEEEECCCEEEEEEHHCCHHHHHHHHHHHHHHHCCCCCEEEEEC
IGYRAIAREQQD
CCHHHHHCCCCC
>Mature Secondary Structure
MKVLVIGGGGREHALAWKAAQSAQVETVYVAPGNAGTALEPNVENLAISATDIPALLDFA
CEEEEEECCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHH
KTNKIELTIVGPEAPLVMGVVDAFNAAGLPIFGPTKAAAQLEGSKAFTKDFLARHNIPTA
CCCEEEEEEECCCCCEEHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCC
GYKNCTEISDAKAFVRELTATTGYPVVIKADGLAAGKGVIIAQDQAEADAAIDDMLAGNK
CCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCC
FGDAGSRVVIEEFLKGEEASFIVMVDGKNILPMATSQDHKARDNGDYGPNTGGMGAYSPA
CCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
PVVTQAVHDWTVANVIRPTVDGMAAEGNVYTGFLYAGLMISPDGSAKVLEYNCRFGDPET
CHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCC
QPIMMRLKSDLVELCLAATRGELDKVTAEYDERAAVGVVLAAGGYPDEYRKFDVIDGLSL
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEEECCCCHHHCEEEEECCCEE
GDNNGKVFHAGTEMKDGHVVTNGGRVLCATALGNTVTEAQKAAYKLVDEIHWDDVYFRTD
CCCCCEEEECCCCCCCCEEEECCCEEEEEEHHCCHHHHHHHHHHHHHHHCCCCCEEEEEC
IGYRAIAREQQD
CCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]