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The map label for this gene is purH [H]

Identifier: 160877140

GI number: 160877140

Start: 4747653

End: 4749251

Strand: Direct

Name: purH [H]

Synonym: Sbal195_4036

Alternate gene names: 160877140

Gene position: 4747653-4749251 (Clockwise)

Preceding gene: 160877137

Following gene: 160877141

Centisome position: 88.79

GC content: 51.53

Gene sequence:

>1599_bases
ATGACTGCTGCAAATAATGCCAGACCCATTCGTCGCGCGCTGTTAAGCGTTTCAGATAAAACCGGAATTCTCGAGTTCGC
CAAAGCACTTCACGCCCAAGGTGTGGAACTGTTATCAACTGGCGGCACCGCTCGCCTGTTAGCGGATAACGGCGTGCCTG
TTATCGAAGTATCTGATTACACAGGACACCCTGAGATCATGGACGGTCGCGTTAAGACGCTGCACCCTAAAGTGCACGGC
GGCATTTTGGCGCGCCGCGGTCTTGATGAAAGCGTTATGGCCGACAACAATATCAACGCCATCGATCTGGTTGCGGTTAA
CCTTTATCCTTTCGCTGAAACCGTAGCTAAAGCCGGTTGTACCTTAGAGGACGCTATCGAAAATATCGATATTGGCGGCC
CAACTATGGTGCGCGCAGCGGCAAAAAACCACAAAGACGTCACCATAGTCGTTAATGCCGCCGATTACTCACGCGTACTG
GCAGAAATGACGGCTAACAATGGCAGCACGACCCATGCGACGCGTTTCGACTTAGCGATTGCAGCCTTTGAGCACACTGC
GGGTTACGATGGCATGATCGCCAACTACTTCGGCACTATGGTTCCTGCACACAGCACGGACGAATGCTTTGCTGATTCTA
AGTTCCCACGCACGTTCAACACCCAATTAGTGAAGAAGCAAGACTTACGCTATGGCGAAAACAGCCATCAAGCGGCGGCC
TTCTATGTCGACACTAAAATTGATGAAGCCTCTGTGGCGACGGCAATTCAGTTGCAAGGCAAAGCTTTGTCTTACAACAA
CATTGCCGATACAGACGCCGCTCTTGAGTGCGTAAAAGAATTCTTGGAACCTGCCTGCGTTATCGTTAAACACGCTAACC
CATGTGGTGTGGCCTTAGGTAAAGACTTGCTCGATGCCTATAACCGCGCTTATCAAACAGACCCAACGTCAGCCTTCGGT
GGCATTATTGCTTTCAACGGCGAGTTAGATGCCGCGACGGCGAGTGCTATCGTTGAGCGTCAATTCGTTGAAGTGATTAT
CGCCCCAAGCGTCAGCCAAGCGGCGCGCGATGTGGTGGCGAAAAAGACCAACGTACGTTTATTGGAATGTGGTCAGTGGA
ACACTAAGACCCAAACCTTAGACTTCAAACGCGTTAACGGCGGCTTGTTAGTACAAGATCGCGACCAAGGCATGGTCGGC
TTAGAAGACATCAAAGTGGTTTCTAAACGTCAACCAACTGCAAGCGAACTGAAAGACTTAATGTTCTGCTGGAAAGTAGC
GAAATTCGTTAAATCTAACGCCATCGTTTATGCAAAAGACGGCATGACTATCGGTGTCGGCGCAGGCCAAATGAGCCGCG
TTTACAGCGCTAAAATCGCTGGCATCAAGGCCGCCGACGAAGGTTTAGAAGTAGTGAACTCTGTGATGGCATCCGATGCT
TTCTTCCCCTTCCGTGACGGTATCGATGCCGCAGCGGCTGCGGGCATTAGCTGCATCATCCAACCGGGTGGCTCAATGCG
CGATGCAGAAATCATCGCCGCAGCAGACGAGCACGGCATGGCCATGGTGATGACGGGCATGCGCCACTTCCGTCACTAA

Upstream 100 bases:

>100_bases
AAAAATAGGACCAAGGGACTTTTGGAAAGCGGTGCAGCGCTAAGGCTGTTCTGTTTTCCGAAAGATCCTTGAAACTAAAA
TAACTGGACCCTGTACCCAA

Downstream 100 bases:

>100_bases
TCGCCACTAAGCAGCCAGCACTCAATCAGAACTGGCTGCTTTTTTGTTTTACCACTCTTTGTTTTACCGCACTCTGGTTT
CGACCACAGTGTGCCTACAC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTAANNARPIRRALLSVSDKTGILEFAKALHAQGVELLSTGGTARLLADNGVPVIEVSDYTGHPEIMDGRVKTLHPKVHG
GILARRGLDESVMADNNINAIDLVAVNLYPFAETVAKAGCTLEDAIENIDIGGPTMVRAAAKNHKDVTIVVNAADYSRVL
AEMTANNGSTTHATRFDLAIAAFEHTAGYDGMIANYFGTMVPAHSTDECFADSKFPRTFNTQLVKKQDLRYGENSHQAAA
FYVDTKIDEASVATAIQLQGKALSYNNIADTDAALECVKEFLEPACVIVKHANPCGVALGKDLLDAYNRAYQTDPTSAFG
GIIAFNGELDAATASAIVERQFVEVIIAPSVSQAARDVVAKKTNVRLLECGQWNTKTQTLDFKRVNGGLLVQDRDQGMVG
LEDIKVVSKRQPTASELKDLMFCWKVAKFVKSNAIVYAKDGMTIGVGAGQMSRVYSAKIAGIKAADEGLEVVNSVMASDA
FFPFRDGIDAAAAAGISCIIQPGGSMRDAEIIAAADEHGMAMVMTGMRHFRH

Sequences:

>Translated_532_residues
MTAANNARPIRRALLSVSDKTGILEFAKALHAQGVELLSTGGTARLLADNGVPVIEVSDYTGHPEIMDGRVKTLHPKVHG
GILARRGLDESVMADNNINAIDLVAVNLYPFAETVAKAGCTLEDAIENIDIGGPTMVRAAAKNHKDVTIVVNAADYSRVL
AEMTANNGSTTHATRFDLAIAAFEHTAGYDGMIANYFGTMVPAHSTDECFADSKFPRTFNTQLVKKQDLRYGENSHQAAA
FYVDTKIDEASVATAIQLQGKALSYNNIADTDAALECVKEFLEPACVIVKHANPCGVALGKDLLDAYNRAYQTDPTSAFG
GIIAFNGELDAATASAIVERQFVEVIIAPSVSQAARDVVAKKTNVRLLECGQWNTKTQTLDFKRVNGGLLVQDRDQGMVG
LEDIKVVSKRQPTASELKDLMFCWKVAKFVKSNAIVYAKDGMTIGVGAGQMSRVYSAKIAGIKAADEGLEVVNSVMASDA
FFPFRDGIDAAAAAGISCIIQPGGSMRDAEIIAAADEHGMAMVMTGMRHFRH
>Mature_531_residues
TAANNARPIRRALLSVSDKTGILEFAKALHAQGVELLSTGGTARLLADNGVPVIEVSDYTGHPEIMDGRVKTLHPKVHGG
ILARRGLDESVMADNNINAIDLVAVNLYPFAETVAKAGCTLEDAIENIDIGGPTMVRAAAKNHKDVTIVVNAADYSRVLA
EMTANNGSTTHATRFDLAIAAFEHTAGYDGMIANYFGTMVPAHSTDECFADSKFPRTFNTQLVKKQDLRYGENSHQAAAF
YVDTKIDEASVATAIQLQGKALSYNNIADTDAALECVKEFLEPACVIVKHANPCGVALGKDLLDAYNRAYQTDPTSAFGG
IIAFNGELDAATASAIVERQFVEVIIAPSVSQAARDVVAKKTNVRLLECGQWNTKTQTLDFKRVNGGLLVQDRDQGMVGL
EDIKVVSKRQPTASELKDLMFCWKVAKFVKSNAIVYAKDGMTIGVGAGQMSRVYSAKIAGIKAADEGLEVVNSVMASDAF
FPFRDGIDAAAAAGISCIIQPGGSMRDAEIIAAADEHGMAMVMTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=488, Percent_Identity=38.3196721311475, Blast_Score=275, Evalue=1e-73,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=74.8571428571429, Blast_Score=830, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=510, Percent_Identity=33.7254901960784, Blast_Score=241, Evalue=9e-64,
Organism=Caenorhabditis elegans, GI71985574, Length=339, Percent_Identity=27.7286135693215, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71985556, Length=130, Percent_Identity=33.8461538461538, Blast_Score=76, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323056, Length=612, Percent_Identity=33.9869281045752, Blast_Score=283, Evalue=6e-77,
Organism=Saccharomyces cerevisiae, GI6323768, Length=486, Percent_Identity=36.6255144032922, Blast_Score=268, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24649832, Length=612, Percent_Identity=36.1111111111111, Blast_Score=304, Evalue=1e-82,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56894; Mature: 56763

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAANNARPIRRALLSVSDKTGILEFAKALHAQGVELLSTGGTARLLADNGVPVIEVSDY
CCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEECCC
TGHPEIMDGRVKTLHPKVHGGILARRGLDESVMADNNINAIDLVAVNLYPFAETVAKAGC
CCCCCCCCCCEEEECCHHHCCHHHHCCCCHHHHCCCCCCEEEEEEEECCHHHHHHHHCCC
TLEDAIENIDIGGPTMVRAAAKNHKDVTIVVNAADYSRVLAEMTANNGSTTHATRFDLAI
CHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCCEEEEHEEEE
AAFEHTAGYDGMIANYFGTMVPAHSTDECFADSKFPRTFNTQLVKKQDLRYGENSHQAAA
EEEHHCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCEEE
FYVDTKIDEASVATAIQLQGKALSYNNIADTDAALECVKEFLEPACVIVKHANPCGVALG
EEEECCCCCHHHEEEEEECCCEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCHHHH
KDLLDAYNRAYQTDPTSAFGGIIAFNGELDAATASAIVERQFVEVIIAPSVSQAARDVVA
HHHHHHHHHHCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHH
KKTNVRLLECGQWNTKTQTLDFKRVNGGLLVQDRDQGMVGLEDIKVVSKRQPTASELKDL
HCCCEEEEECCCCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHH
MFCWKVAKFVKSNAIVYAKDGMTIGVGAGQMSRVYSAKIAGIKAADEGLEVVNSVMASDA
HHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
FFPFRDGIDAAAAAGISCIIQPGGSMRDAEIIAAADEHGMAMVMTGMRHFRH
CCCCCCCCCHHHHCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TAANNARPIRRALLSVSDKTGILEFAKALHAQGVELLSTGGTARLLADNGVPVIEVSDY
CCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEECCC
TGHPEIMDGRVKTLHPKVHGGILARRGLDESVMADNNINAIDLVAVNLYPFAETVAKAGC
CCCCCCCCCCEEEECCHHHCCHHHHCCCCHHHHCCCCCCEEEEEEEECCHHHHHHHHCCC
TLEDAIENIDIGGPTMVRAAAKNHKDVTIVVNAADYSRVLAEMTANNGSTTHATRFDLAI
CHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCCCCCEEEEHEEEE
AAFEHTAGYDGMIANYFGTMVPAHSTDECFADSKFPRTFNTQLVKKQDLRYGENSHQAAA
EEEHHCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCEEE
FYVDTKIDEASVATAIQLQGKALSYNNIADTDAALECVKEFLEPACVIVKHANPCGVALG
EEEECCCCCHHHEEEEEECCCEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCHHHH
KDLLDAYNRAYQTDPTSAFGGIIAFNGELDAATASAIVERQFVEVIIAPSVSQAARDVVA
HHHHHHHHHHCCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHH
KKTNVRLLECGQWNTKTQTLDFKRVNGGLLVQDRDQGMVGLEDIKVVSKRQPTASELKDL
HCCCEEEEECCCCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHH
MFCWKVAKFVKSNAIVYAKDGMTIGVGAGQMSRVYSAKIAGIKAADEGLEVVNSVMASDA
HHHHHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
FFPFRDGIDAAAAAGISCIIQPGGSMRDAEIIAAADEHGMAMVMTGMRHFRH
CCCCCCCCCHHHHCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]