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The map label for this gene is miaA [H]

Identifier: 160876998

GI number: 160876998

Start: 4587884

End: 4588774

Strand: Reverse

Name: miaA [H]

Synonym: Sbal195_3894

Alternate gene names: 160876998

Gene position: 4588774-4587884 (Counterclockwise)

Preceding gene: 160876999

Following gene: 160876997

Centisome position: 85.82

GC content: 48.82

Gene sequence:

>891_bases
ATGGGGCCAACGGCCTCAGGCAAAACTGCTTTAGCATTAGAATTGGCCGAAAAGCATAACTGTGAAATCATTTCGGTCGA
TTCAGCATTAATTTATCGCGGTATGGACATTGGCAGCGCCAAGCCTTCCGCCGACGAACTGGCCCGTGGGCCACACAGAT
TGATCGACATTCGCGATCCTAGCGAAAGTTATTCGGCAGCCGACTTTAGAGCCGATGCGATAGCCGAGATAGAACAGATT
GTCAGCATGGGTAAAACGCCAGTATTAGTTGGCGGAACCATGATGTATTTCAAAGCGTTACTCGAAGGTTTGTCACCTTT
GCCGAGTGCGGACGAGGCCATTAGAGCCGAGATCCAAGCTGAGGCGGATGAAAAGGGATGGGAAGCACTGCATGACCAAT
TAAGGGAGATAGATCCCGTGTCGGCAGAAAGAATTCATCCTAATGATCCACAAAGACTTTCAAGAGCGCTGGAAGTCTAT
AGAATAAGCGGCAAGAGCATGACTGAGCTGACTCAGACCAAGTCTGCTCCCTTGCCCTATGACGTAGTGCAGTTTGCTAT
TGCACCGCGTGAGCGCAAAGTGTTACATGACTTAATTGCGCAACGTTTTGCGATTATGCTCAAGCAGGGCTTTCTTGAGG
AAGTCACAGAGCTGAAAGCCCGTGGAGACCTGCATTTAGACTTGCCTTCAATGCGTTGTGTGGGCTATCGACAGTGTTGG
CAATATCTCGATGGTGAGTTTGACTATGATACTATGGTCGAAAAAGCGGTTGCTGCTACTAGGCAATTGGCTAAGCGTCA
ATTAACATGGTTGCGCAGTTGGCCAGAGTTGAATTGGCTCGAAAGTGGTGCTGAGGGGAATTTAGTTACACTTATGCGTC
AATGCCGCTAG

Upstream 100 bases:

>100_bases
AAACAGCTTATTGCCAACAAAGCAATATTACTACCTTGGCAATCGTGGCTAGAAGAGCAAGCAAGTGAATAAAGAATTAC
AGCCTAAAGTGATATTTTTG

Downstream 100 bases:

>100_bases
CTTATTAGCCCTTGCTGTATAATACTAAAACTAAACAAAAAACTATCACTGATATCGATTTATTTTATATAAATTAAAAA
GGAAATAATAAAATGGCTAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MGPTASGKTALALELAEKHNCEIISVDSALIYRGMDIGSAKPSADELARGPHRLIDIRDPSESYSAADFRADAIAEIEQI
VSMGKTPVLVGGTMMYFKALLEGLSPLPSADEAIRAEIQAEADEKGWEALHDQLREIDPVSAERIHPNDPQRLSRALEVY
RISGKSMTELTQTKSAPLPYDVVQFAIAPRERKVLHDLIAQRFAIMLKQGFLEEVTELKARGDLHLDLPSMRCVGYRQCW
QYLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLMRQCR

Sequences:

>Translated_296_residues
MGPTASGKTALALELAEKHNCEIISVDSALIYRGMDIGSAKPSADELARGPHRLIDIRDPSESYSAADFRADAIAEIEQI
VSMGKTPVLVGGTMMYFKALLEGLSPLPSADEAIRAEIQAEADEKGWEALHDQLREIDPVSAERIHPNDPQRLSRALEVY
RISGKSMTELTQTKSAPLPYDVVQFAIAPRERKVLHDLIAQRFAIMLKQGFLEEVTELKARGDLHLDLPSMRCVGYRQCW
QYLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLMRQCR
>Mature_295_residues
GPTASGKTALALELAEKHNCEIISVDSALIYRGMDIGSAKPSADELARGPHRLIDIRDPSESYSAADFRADAIAEIEQIV
SMGKTPVLVGGTMMYFKALLEGLSPLPSADEAIRAEIQAEADEKGWEALHDQLREIDPVSAERIHPNDPQRLSRALEVYR
ISGKSMTELTQTKSAPLPYDVVQFAIAPRERKVLHDLIAQRFAIMLKQGFLEEVTELKARGDLHLDLPSMRCVGYRQCWQ
YLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLMRQCR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=304, Percent_Identity=27.3026315789474, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1790613, Length=282, Percent_Identity=68.0851063829787, Blast_Score=400, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25144712, Length=270, Percent_Identity=29.6296296296296, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324848, Length=282, Percent_Identity=27.6595744680851, Blast_Score=123, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24649830, Length=236, Percent_Identity=31.3559322033898, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 33139; Mature: 33008

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPTASGKTALALELAEKHNCEIISVDSALIYRGMDIGSAKPSADELARGPHRLIDIRDP
CCCCCCCCCEEEEEHHHHCCCCEEEECHHHHHCCCCCCCCCCCHHHHHCCCCCEEEECCC
SESYSAADFRADAIAEIEQIVSMGKTPVLVGGTMMYFKALLEGLSPLPSADEAIRAEIQA
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EADEKGWEALHDQLREIDPVSAERIHPNDPQRLSRALEVYRISGKSMTELTQTKSAPLPY
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCH
DVVQFAIAPRERKVLHDLIAQRFAIMLKQGFLEEVTELKARGDLHLDLPSMRCVGYRQCW
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHH
QYLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLMRQCR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure 
GPTASGKTALALELAEKHNCEIISVDSALIYRGMDIGSAKPSADELARGPHRLIDIRDP
CCCCCCCCEEEEEHHHHCCCCEEEECHHHHHCCCCCCCCCCCHHHHHCCCCCEEEECCC
SESYSAADFRADAIAEIEQIVSMGKTPVLVGGTMMYFKALLEGLSPLPSADEAIRAEIQA
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EADEKGWEALHDQLREIDPVSAERIHPNDPQRLSRALEVYRISGKSMTELTQTKSAPLPY
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCH
DVVQFAIAPRERKVLHDLIAQRFAIMLKQGFLEEVTELKARGDLHLDLPSMRCVGYRQCW
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHH
QYLDGEFDYDTMVEKAVAATRQLAKRQLTWLRSWPELNWLESGAEGNLVTLMRQCR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA