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The map label for this gene is nrfA [H]

Identifier: 160876938

GI number: 160876938

Start: 4514335

End: 4515738

Strand: Reverse

Name: nrfA [H]

Synonym: Sbal195_3834

Alternate gene names: 160876938

Gene position: 4515738-4514335 (Counterclockwise)

Preceding gene: 160876941

Following gene: 160876936

Centisome position: 84.45

GC content: 47.79

Gene sequence:

>1404_bases
ATGATGAAGAAGATGACAGGTAAGTCCTTTGCACTAAGTGCACTGGTTGCCGCCAGCTTCATGGCGGCGGGCGCAATGGC
GAGTGATAAAACTGAGCCTCGCAACGAAGTTTATAAAGACAAATTTGCTAATCAATACGCTAGCTGGCATGACACAGATA
AAAGCACTGAAAATACCGATGTGTTAGCCTCGGACCCGAGCTTAGTCGTGCTGTGGGCAGGTTATGGTTTTGCGAAAGAT
TACAACGCACCACGTGGCCACATGTATGCCATTACGGATGTGCGTAACACTCTGCGAACTGGCGCACCAGCCAACGCGGA
AGATGGTCCAATGCCTATGGCTTGTTGGAGCTGTAAGAGCCCTGATGTACCTCGCTTAATTGAAGAGCAAGGCGAAGATG
GTTACTTCAAAGGCAAATGGGCAAAAGGCGGCCCAGAAGTGGTGAACACCATAGGCTGCGGTGACTGCCATGAAAAAGGC
ACGCCTAAACTGCGTATCTCTCGCCCCTTTGCTGAACGTGGTATGGAAGCATTAGGTACACCGTTTGCCAAAGCGTCTAA
AAAAGACAAGCAATCTATGGTCTGCGGTCAGTGTCACGTTGAATACTATTTCGAGAAAAAAGAAGGCAGAAAAGGTTTCG
TTAAATTCCCTTGGGATATGGGCACAACCGTTGAGCAAATGGAAACCTATTACGACGGTATTGAGTTCTCCGACTGGACG
CATGCGCTGTCTAAAACCCCTATGTTAAAAGCGCAGCACCCTGGTTATGAAACTTGGAAACTCGGTGTTCATGGTAAGAA
CGATGTGAGCTGTGTTGATTGTCACATGCCAAAAGTGACTAACGACAAGGGTCGTAAGTACACAGATCATAAAGTGGGTA
ACCCATTTGATCGTTTTGAAGAAACCTGCGCGACGTGCCACAGCCAAAGTAAAGAGTTCTTAGTCGGCTTGACCAACGAA
CGTAAAGCGAAAGTAAAAGAGCTTAAAGGCCGCGCCGAAGCACAACTGGTGAAAGCGCACTTTGAAGCCGCAGCCGCTTG
GAAAGCGGGTGCAACTGAAGCGGAAATGAAGCCTATTCTAACCGATATCCGTCATTCACAATGGCGTTGGGACTATGCGA
TTGCTTCACACGGCGCTGCTGCGCATGCCCCTGAAGAAGTCTTACGCATCCTAGGCACAGCGGTAGATAAAGCGGCAGAT
GCGCGGGTTAAATTAGCGCAACTGTTAGCGAAAAAAGGCATCACAGATCCTATCGCGATCCCTGATATTTCTACTAAAGC
CAAGGCACAAGCCGCTTTAGGTATGGACATGGAAAAAATGAATGCAGAGAAAGAAGCATTTAAGAAAGACATGTTACCTA
AGTGGGATGAAGAAGCGAAAAAACGCGAAGCCACTTATAAATAG

Upstream 100 bases:

>100_bases
CTAGATACCCCCTAAGAGGTATATGTTTAGCGGGATTAAGTCACTAAGTTTGACCATGGAGATGTCGTGATTTACAACAA
TAATGCAACACGGAGATGAG

Downstream 100 bases:

>100_bases
TGTTTAACAAATTGTTTTTAACCTGAGCTAACCCCTCATGTAAGCAAAAGCCCTCGCTGACTCAGCGAGGGCTTTTTTAT
GGGAGTGAATGTCAACGTCA

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYASWHDTDKSTENTDVLASDPSLVVLWAGYGFAKD
YNAPRGHMYAITDVRNTLRTGAPANAEDGPMPMACWSCKSPDVPRLIEEQGEDGYFKGKWAKGGPEVVNTIGCGDCHEKG
TPKLRISRPFAERGMEALGTPFAKASKKDKQSMVCGQCHVEYYFEKKEGRKGFVKFPWDMGTTVEQMETYYDGIEFSDWT
HALSKTPMLKAQHPGYETWKLGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGNPFDRFEETCATCHSQSKEFLVGLTNE
RKAKVKELKGRAEAQLVKAHFEAAAAWKAGATEAEMKPILTDIRHSQWRWDYAIASHGAAAHAPEEVLRILGTAVDKAAD
ARVKLAQLLAKKGITDPIAIPDISTKAKAQAALGMDMEKMNAEKEAFKKDMLPKWDEEAKKREATYK

Sequences:

>Translated_467_residues
MMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYASWHDTDKSTENTDVLASDPSLVVLWAGYGFAKD
YNAPRGHMYAITDVRNTLRTGAPANAEDGPMPMACWSCKSPDVPRLIEEQGEDGYFKGKWAKGGPEVVNTIGCGDCHEKG
TPKLRISRPFAERGMEALGTPFAKASKKDKQSMVCGQCHVEYYFEKKEGRKGFVKFPWDMGTTVEQMETYYDGIEFSDWT
HALSKTPMLKAQHPGYETWKLGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGNPFDRFEETCATCHSQSKEFLVGLTNE
RKAKVKELKGRAEAQLVKAHFEAAAAWKAGATEAEMKPILTDIRHSQWRWDYAIASHGAAAHAPEEVLRILGTAVDKAAD
ARVKLAQLLAKKGITDPIAIPDISTKAKAQAALGMDMEKMNAEKEAFKKDMLPKWDEEAKKREATYK
>Mature_467_residues
MMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYASWHDTDKSTENTDVLASDPSLVVLWAGYGFAKD
YNAPRGHMYAITDVRNTLRTGAPANAEDGPMPMACWSCKSPDVPRLIEEQGEDGYFKGKWAKGGPEVVNTIGCGDCHEKG
TPKLRISRPFAERGMEALGTPFAKASKKDKQSMVCGQCHVEYYFEKKEGRKGFVKFPWDMGTTVEQMETYYDGIEFSDWT
HALSKTPMLKAQHPGYETWKLGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGNPFDRFEETCATCHSQSKEFLVGLTNE
RKAKVKELKGRAEAQLVKAHFEAAAAWKAGATEAEMKPILTDIRHSQWRWDYAIASHGAAAHAPEEVLRILGTAVDKAAD
ARVKLAQLLAKKGITDPIAIPDISTKAKAQAALGMDMEKMNAEKEAFKKDMLPKWDEEAKKREATYK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=438, Percent_Identity=64.3835616438356, Blast_Score=612, Evalue=1e-176,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 51872; Mature: 51872

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYASWHDTDKSTENTD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VLASDPSLVVLWAGYGFAKDYNAPRGHMYAITDVRNTLRTGAPANAEDGPMPMACWSCKS
EEECCCCEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCEEEECCCC
PDVPRLIEEQGEDGYFKGKWAKGGPEVVNTIGCGDCHEKGTPKLRISRPFAERGMEALGT
CCCCHHHHHCCCCCEEECCCCCCCHHHHHHCCCCCCCCCCCCCEEEECCHHHHCHHHHCC
PFAKASKKDKQSMVCGQCHVEYYFEKKEGRKGFVKFPWDMGTTVEQMETYYDGIEFSDWT
CHHHHCCHHHHHHHHHHHHEEEEEECCCCCCCCEECCCCCCCHHHHHHHHHCCCCCCHHH
HALSKTPMLKAQHPGYETWKLGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGNPFDRFE
HHHHCCCCCEECCCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCEECCCCCCCHHHHHH
ETCATCHSQSKEFLVGLTNERKAKVKELKGRAEAQLVKAHFEAAAAWKAGATEAEMKPIL
HHHHHHHCCCCCEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TDIRHSQWRWDYAIASHGAAAHAPEEVLRILGTAVDKAADARVKLAQLLAKKGITDPIAI
HHHHHCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PDISTKAKAQAALGMDMEKMNAEKEAFKKDMLPKWDEEAKKREATYK
CCCCCHHHHHHHHCCCHHHHCHHHHHHHHHCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MMKKMTGKSFALSALVAASFMAAGAMASDKTEPRNEVYKDKFANQYASWHDTDKSTENTD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VLASDPSLVVLWAGYGFAKDYNAPRGHMYAITDVRNTLRTGAPANAEDGPMPMACWSCKS
EEECCCCEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCEEEECCCC
PDVPRLIEEQGEDGYFKGKWAKGGPEVVNTIGCGDCHEKGTPKLRISRPFAERGMEALGT
CCCCHHHHHCCCCCEEECCCCCCCHHHHHHCCCCCCCCCCCCCEEEECCHHHHCHHHHCC
PFAKASKKDKQSMVCGQCHVEYYFEKKEGRKGFVKFPWDMGTTVEQMETYYDGIEFSDWT
CHHHHCCHHHHHHHHHHHHEEEEEECCCCCCCCEECCCCCCCHHHHHHHHHCCCCCCHHH
HALSKTPMLKAQHPGYETWKLGVHGKNDVSCVDCHMPKVTNDKGRKYTDHKVGNPFDRFE
HHHHCCCCCEECCCCCCEEEECCCCCCCCEEEECCCCCCCCCCCCEECCCCCCCHHHHHH
ETCATCHSQSKEFLVGLTNERKAKVKELKGRAEAQLVKAHFEAAAAWKAGATEAEMKPIL
HHHHHHHCCCCCEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TDIRHSQWRWDYAIASHGAAAHAPEEVLRILGTAVDKAADARVKLAQLLAKKGITDPIAI
HHHHHCCCEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PDISTKAKAQAALGMDMEKMNAEKEAFKKDMLPKWDEEAKKREATYK
CCCCCHHHHHHHHCCCHHHHCHHHHHHHHHCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA