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The map label for this gene is prfA [H]

Identifier: 160876841

GI number: 160876841

Start: 4403355

End: 4404446

Strand: Reverse

Name: prfA [H]

Synonym: Sbal195_3737

Alternate gene names: 160876841

Gene position: 4404446-4403355 (Counterclockwise)

Preceding gene: 160876842

Following gene: 160876840

Centisome position: 82.37

GC content: 50.18

Gene sequence:

>1092_bases
ATGAAGGAATCCGTTATCCGCAAGCTGGAAGGCTTGCTCGAGCGCAATGAAGAAGTCATGGCTTTGCTGGGTGATGCTAG
CGTCATTTCTGATCAAGACAGGTTTCGCGCGTTATCGAAAGAATATGCCCAGTTAGAAGACGTTGTTGCTGGCTTTAAAG
CTTATCAGCAAGCGCAGGTCGATCTCGATTCTGCCAAAGAAATGCTGGAAGAAGACGATGCTGAAATGCGCGAAATGGCG
CAGGAAGAGATGAAAGCCGCTAAGGCTAAGCTCGAACATCTTGAAGATGAACTGCAAATTCTATTACTGCCAAAAGATCC
TGACGACGATAAAAACGCTTTCGTTGAGATCCGTGCGGGTGCAGGCGGCGATGAAGCGGCCATTTTTGCAGGTGACTTAT
TCCGCATGTACAGCCGTTATGCCGAAGCAAATCGCTGGCAGATCGAAATCATGAGCTGTAACGAAGGCGAACATGGTGGC
TTCAAAGAAGTGATCATGAAGGTGAGTGGCGACGGCGTATACGGCAAACTTAAGTTTGAGTCTGGCGGTCACCGCGTACA
ACGTGTGCCAGAAACCGAATCTCAAGGCCGTGTGCATACTTCAGCGGTTACTGTGGTTGTATTACATGAAGTGCCTGAAG
CAGAAGCGATTTCAATCAATCCAGCCGATCTGAAAGTTGATACTTTCCGTTCATCGGGCGCGGGTGGTCAGCACGTTAAC
AAAACCGATTCTGCCATTCGTATTACCCACATTCCAACAGGGATCGTGGTGGAATGCCAAGATCAACGTTCGCAACATAA
GAACCGTGCCCAAGCCATGAGTGTATTGGCCGCACGTATTCAAGCTGTTGAAGACGAAAAACGCCGTAGCGCCGAAGAAT
CGACCCGTCGTAGTCTGGTTGCCAGTGGCGATCGCTCCGAACGTGTGCGTACTTACAACTTCCCGCAGGGCCGCGTGAGC
GAACACCGCATCAACTTGACCTTATATCGCTTGAACGAAGTGATGGAAGGCGATCTCGATGCTATTTTATTGCCTCTGAT
GCAAGAGCATCAAGCCGATCAGTTAGCCGCACTGGCTGACGAGCAGGGGTAG

Upstream 100 bases:

>100_bases
CACAGGCCCTCACGGCAGCGAGTCGTCAGGGCGATTTGAATACTCTCGGTCAGTTAAGATCAGCGCTTGGATTAGATAAA
AACTAAGGTTAGCGAGTTAA

Downstream 100 bases:

>100_bases
TTTTGTCAGATCAATCTAGCATAGCCGAAGCCCTGCAATGGGCTTATGTGCAGTTAGCATCAACCTCTGAATCCGCTCAT
CTGGATGCAGAGGTTTTGTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKESVIRKLEGLLERNEEVMALLGDASVISDQDRFRALSKEYAQLEDVVAGFKAYQQAQVDLDSAKEMLEEDDAEMREMA
QEEMKAAKAKLEHLEDELQILLLPKDPDDDKNAFVEIRAGAGGDEAAIFAGDLFRMYSRYAEANRWQIEIMSCNEGEHGG
FKEVIMKVSGDGVYGKLKFESGGHRVQRVPETESQGRVHTSAVTVVVLHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRSLVASGDRSERVRTYNFPQGRVS
EHRINLTLYRLNEVMEGDLDAILLPLMQEHQADQLAALADEQG

Sequences:

>Translated_363_residues
MKESVIRKLEGLLERNEEVMALLGDASVISDQDRFRALSKEYAQLEDVVAGFKAYQQAQVDLDSAKEMLEEDDAEMREMA
QEEMKAAKAKLEHLEDELQILLLPKDPDDDKNAFVEIRAGAGGDEAAIFAGDLFRMYSRYAEANRWQIEIMSCNEGEHGG
FKEVIMKVSGDGVYGKLKFESGGHRVQRVPETESQGRVHTSAVTVVVLHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRSLVASGDRSERVRTYNFPQGRVS
EHRINLTLYRLNEVMEGDLDAILLPLMQEHQADQLAALADEQG
>Mature_363_residues
MKESVIRKLEGLLERNEEVMALLGDASVISDQDRFRALSKEYAQLEDVVAGFKAYQQAQVDLDSAKEMLEEDDAEMREMA
QEEMKAAKAKLEHLEDELQILLLPKDPDDDKNAFVEIRAGAGGDEAAIFAGDLFRMYSRYAEANRWQIEIMSCNEGEHGG
FKEVIMKVSGDGVYGKLKFESGGHRVQRVPETESQGRVHTSAVTVVVLHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRSLVASGDRSERVRTYNFPQGRVS
EHRINLTLYRLNEVMEGDLDAILLPLMQEHQADQLAALADEQG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=41.7808219178082, Blast_Score=261, Evalue=8e-70,
Organism=Homo sapiens, GI34577120, Length=346, Percent_Identity=39.8843930635838, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=40.0966183574879, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI1787462, Length=362, Percent_Identity=71.8232044198895, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI2367172, Length=333, Percent_Identity=39.3393393393393, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=336, Percent_Identity=33.9285714285714, Blast_Score=184, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=313, Percent_Identity=42.8115015974441, Blast_Score=249, Evalue=4e-67,
Organism=Drosophila melanogaster, GI19921226, Length=331, Percent_Identity=39.8791540785499, Blast_Score=218, Evalue=6e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40492; Mature: 40492

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKESVIRKLEGLLERNEEVMALLGDASVISDQDRFRALSKEYAQLEDVVAGFKAYQQAQV
CCHHHHHHHHHHHHCCHHHEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DLDSAKEMLEEDDAEMREMAQEEMKAAKAKLEHLEDELQILLLPKDPDDDKNAFVEIRAG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEEC
AGGDEAAIFAGDLFRMYSRYAEANRWQIEIMSCNEGEHGGFKEVIMKVSGDGVYGKLKFE
CCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEEC
SGGHRVQRVPETESQGRVHTSAVTVVVLHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
CCCCCEEECCCCCCCCCEEEEEEEEEEEECCCCCCEEECCCCCEEEEECCCCCCCCCCCC
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRSLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGDRSERVRTYNFPQGRVSEHRINLTLYRLNEVMEGDLDAILLPLMQEHQADQLAALAD
HCCCCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHHCCHHHHHHHHHHHCCHHHHHHHHC
EQG
CCC
>Mature Secondary Structure
MKESVIRKLEGLLERNEEVMALLGDASVISDQDRFRALSKEYAQLEDVVAGFKAYQQAQV
CCHHHHHHHHHHHHCCHHHEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DLDSAKEMLEEDDAEMREMAQEEMKAAKAKLEHLEDELQILLLPKDPDDDKNAFVEIRAG
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEEEEC
AGGDEAAIFAGDLFRMYSRYAEANRWQIEIMSCNEGEHGGFKEVIMKVSGDGVYGKLKFE
CCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEEC
SGGHRVQRVPETESQGRVHTSAVTVVVLHEVPEAEAISINPADLKVDTFRSSGAGGQHVN
CCCCCEEECCCCCCCCCEEEEEEEEEEEECCCCCCEEECCCCCEEEEECCCCCCCCCCCC
KTDSAIRITHIPTGIVVECQDQRSQHKNRAQAMSVLAARIQAVEDEKRRSAEESTRRSLV
CCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGDRSERVRTYNFPQGRVSEHRINLTLYRLNEVMEGDLDAILLPLMQEHQADQLAALAD
HCCCCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHHCCHHHHHHHHHHHCCHHHHHHHHC
EQG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA