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The map label for this gene is proS [H]

Identifier: 160876111

GI number: 160876111

Start: 3569342

End: 3571057

Strand: Direct

Name: proS [H]

Synonym: Sbal195_3002

Alternate gene names: 160876111

Gene position: 3569342-3571057 (Clockwise)

Preceding gene: 160876110

Following gene: 160876113

Centisome position: 66.75

GC content: 48.89

Gene sequence:

>1716_bases
ATGCGCGTCAGCAAGTACCTGCTATCAACACAGAAAGAAACTCCTGCCAACGCAGAAGTCATTAGTCATCAATTAATGTT
ACGTGCGGGCATGATCCGTCGTAATGCGTCGGGCCTTTATAGCTATCTCCCCACGGGCTTACGCGTACTGCGTAAAGTCG
AAGCCATTGTTCGTGAAGAAATGAACAAAGCCGGTGCAATTGAAATTCTAATGCCTATGGTGCAACCGGCGGACTTGTGG
GTAGAAACAGGTCGTTGGGAAAAGTTTGGTCCCGAGCTGCTGCGCTTTAAAGATCGTCATAATCGCGACTTCGTACTGGG
CCCAACCCACGAAGAAGTGATCACAGACCTGATCCGTAAAGAAGTCAGCTCATACAAGCAATTACCGCTGAACCTTTACC
AAATCCAAACCAAATTCCGTGACGAAGTTCGTCCACGTTTTGGCATGATGCGCTCACGCGAATTCTTGATGAAAGACGCC
TATTCTTTCCACCTTGACGTCGATACCATGAATGAAACCTATGAGGCCATGTACAACGCCTATAGTAACATTCTAACCCG
CATGGGCTTAGCCTTCCGTCCAGTATTAGCTGATACCGGTTCTATCGGTGGCAGCATGTCACACGAGTTCCACGTTCTGG
CGCAAAGCGGTGAAGATTTAATTGCTTACTCAACGGGCAGCGATTACGCGGCTAACATTGAGAAAGCTGAATCACCAGTG
CCCACTGAGCCTCGCGGCGCAGCCACTGAAGAATTGTGTTTAGTCGATACCCCAAATGCGAAGACCATTGCTGAATTGGT
TGAGCAGTTTGATCTGGATATCACTAAAACCGTCAAAACCTTGATCGTCGTCGGCGCATCAGAAGCGACGCCGTTAGTGG
CACTGATTGTTCGCGGTGACCATGAACTCAATGAAGTTAAAGCCGACAAGTTAGACTTAGTGGCATCACCAGTAGAAATG
GCGCCAGAAGCGCTGATCCGTGACGCCATTGGCGCAGGTCCTGGTTCACTTGGCCCTATCGGTCTTAACATCCCTATCGT
TATCGATCACAGCGTTAGCGTCATGAGCGATTTTGCTGCTGGTGCGAATGTGGATGACAAACATTATTTTGGTATCAACT
GGGAACGCGATCTGCCATTAGCGCAAGTTGCCGATATCCGTAACGTAGTCGAAGGCGAGCCAACACCAGATGGTTCAGGC
ATTTATGCTATGGCACGCGGTATCGAAGTGGGTCATATTTTCCAACTGGGTACTAACTACTCTAAATCGATGAACGCGAC
TGTACTAGACGAAAACGGCAAGTCACAGGTGCTATTGATGGGTTGTTACGGTGTCGGCGTGAGCCGTATTGTGGCCGCAG
CCATCGAGCAAAACTTCGATGACCGTGGCATCATTTGGCCAGAAGCGATTGCACCATTTAGCGTGGGCATTCTGCCAATG
AACATGCACAAGTCGCACCGCGTAACCGATATCGCCGAGCAGTTATACAAAGACTTAAACGAAGCGGGTATCGACGTGTT
ACTGGACGATCGTAAAGAACGCCCAGGTGTGATGTTTGCCGATATGGAACTGATTGGTATCCCACACACAGTGGTGATTG
GTGATCGCAATATCGATGCTGGCGTATTTGAATACAAAAACCGCCGCACCGGTGAAAAGCAAGATATTCCATTTGATCAA
CTGCTCGACTTCCTGAAAAACGCAGTGAAAGGCTAA

Upstream 100 bases:

>100_bases
TTTGTCTCCCAGCTAGTTGAATTTAACCTAAATACGTAGGTGACATCATCAAAGCAGGTGATAAAATGGCGACCATTCAA
CTCAAATTTGGACATCGGTA

Downstream 100 bases:

>100_bases
GCCTCAACGCGAAATATAGAAAAGCTTAACGATCAAAAACGAAACGCACTTAAGCCCAGCTTAAGTGCGTTTTTTATTGG
CTTTTTATTGGACTTTTATT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRVSKYLLSTQKETPANAEVISHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREEMNKAGAIEILMPMVQPADLW
VETGRWEKFGPELLRFKDRHNRDFVLGPTHEEVITDLIRKEVSSYKQLPLNLYQIQTKFRDEVRPRFGMMRSREFLMKDA
YSFHLDVDTMNETYEAMYNAYSNILTRMGLAFRPVLADTGSIGGSMSHEFHVLAQSGEDLIAYSTGSDYAANIEKAESPV
PTEPRGAATEELCLVDTPNAKTIAELVEQFDLDITKTVKTLIVVGASEATPLVALIVRGDHELNEVKADKLDLVASPVEM
APEALIRDAIGAGPGSLGPIGLNIPIVIDHSVSVMSDFAAGANVDDKHYFGINWERDLPLAQVADIRNVVEGEPTPDGSG
IYAMARGIEVGHIFQLGTNYSKSMNATVLDENGKSQVLLMGCYGVGVSRIVAAAIEQNFDDRGIIWPEAIAPFSVGILPM
NMHKSHRVTDIAEQLYKDLNEAGIDVLLDDRKERPGVMFADMELIGIPHTVVIGDRNIDAGVFEYKNRRTGEKQDIPFDQ
LLDFLKNAVKG

Sequences:

>Translated_571_residues
MRVSKYLLSTQKETPANAEVISHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREEMNKAGAIEILMPMVQPADLW
VETGRWEKFGPELLRFKDRHNRDFVLGPTHEEVITDLIRKEVSSYKQLPLNLYQIQTKFRDEVRPRFGMMRSREFLMKDA
YSFHLDVDTMNETYEAMYNAYSNILTRMGLAFRPVLADTGSIGGSMSHEFHVLAQSGEDLIAYSTGSDYAANIEKAESPV
PTEPRGAATEELCLVDTPNAKTIAELVEQFDLDITKTVKTLIVVGASEATPLVALIVRGDHELNEVKADKLDLVASPVEM
APEALIRDAIGAGPGSLGPIGLNIPIVIDHSVSVMSDFAAGANVDDKHYFGINWERDLPLAQVADIRNVVEGEPTPDGSG
IYAMARGIEVGHIFQLGTNYSKSMNATVLDENGKSQVLLMGCYGVGVSRIVAAAIEQNFDDRGIIWPEAIAPFSVGILPM
NMHKSHRVTDIAEQLYKDLNEAGIDVLLDDRKERPGVMFADMELIGIPHTVVIGDRNIDAGVFEYKNRRTGEKQDIPFDQ
LLDFLKNAVKG
>Mature_571_residues
MRVSKYLLSTQKETPANAEVISHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREEMNKAGAIEILMPMVQPADLW
VETGRWEKFGPELLRFKDRHNRDFVLGPTHEEVITDLIRKEVSSYKQLPLNLYQIQTKFRDEVRPRFGMMRSREFLMKDA
YSFHLDVDTMNETYEAMYNAYSNILTRMGLAFRPVLADTGSIGGSMSHEFHVLAQSGEDLIAYSTGSDYAANIEKAESPV
PTEPRGAATEELCLVDTPNAKTIAELVEQFDLDITKTVKTLIVVGASEATPLVALIVRGDHELNEVKADKLDLVASPVEM
APEALIRDAIGAGPGSLGPIGLNIPIVIDHSVSVMSDFAAGANVDDKHYFGINWERDLPLAQVADIRNVVEGEPTPDGSG
IYAMARGIEVGHIFQLGTNYSKSMNATVLDENGKSQVLLMGCYGVGVSRIVAAAIEQNFDDRGIIWPEAIAPFSVGILPM
NMHKSHRVTDIAEQLYKDLNEAGIDVLLDDRKERPGVMFADMELIGIPHTVVIGDRNIDAGVFEYKNRRTGEKQDIPFDQ
LLDFLKNAVKG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=214, Percent_Identity=44.8598130841121, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI1786392, Length=572, Percent_Identity=70.8041958041958, Blast_Score=847, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=253, Percent_Identity=38.7351778656126, Blast_Score=178, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI193203271, Length=105, Percent_Identity=37.1428571428571, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6320931, Length=543, Percent_Identity=30.2025782688766, Blast_Score=238, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24656200, Length=234, Percent_Identity=37.6068376068376, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63264; Mature: 63264

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSKYLLSTQKETPANAEVISHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREE
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
MNKAGAIEILMPMVQPADLWVETGRWEKFGPELLRFKDRHNRDFVLGPTHEEVITDLIRK
HCCCCCHHHHHHHCCCCHHEEECCCCHHCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EVSSYKQLPLNLYQIQTKFRDEVRPRFGMMRSREFLMKDAYSFHLDVDTMNETYEAMYNA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHHHHH
YSNILTRMGLAFRPVLADTGSIGGSMSHEFHVLAQSGEDLIAYSTGSDYAANIEKAESPV
HHHHHHHHCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHCHHHCCCCC
PTEPRGAATEELCLVDTPNAKTIAELVEQFDLDITKTVKTLIVVGASEATPLVALIVRGD
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCEEEEEEECC
HELNEVKADKLDLVASPVEMAPEALIRDAIGAGPGSLGPIGLNIPIVIDHSVSVMSDFAA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHC
GANVDDKHYFGINWERDLPLAQVADIRNVVEGEPTPDGSGIYAMARGIEVGHIFQLGTNY
CCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEEECCCC
SKSMNATVLDENGKSQVLLMGCYGVGVSRIVAAAIEQNFDDRGIIWPEAIAPFSVGILPM
CCCCCEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEE
NMHKSHRVTDIAEQLYKDLNEAGIDVLLDDRKERPGVMFADMELIGIPHTVVIGDRNIDA
CCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECEEEECCCEEEEEECCCCCC
GVFEYKNRRTGEKQDIPFDQLLDFLKNAVKG
CHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVSKYLLSTQKETPANAEVISHQLMLRAGMIRRNASGLYSYLPTGLRVLRKVEAIVREE
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
MNKAGAIEILMPMVQPADLWVETGRWEKFGPELLRFKDRHNRDFVLGPTHEEVITDLIRK
HCCCCCHHHHHHHCCCCHHEEECCCCHHCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EVSSYKQLPLNLYQIQTKFRDEVRPRFGMMRSREFLMKDAYSFHLDVDTMNETYEAMYNA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHHHHH
YSNILTRMGLAFRPVLADTGSIGGSMSHEFHVLAQSGEDLIAYSTGSDYAANIEKAESPV
HHHHHHHHCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCHHCHHHCCCCC
PTEPRGAATEELCLVDTPNAKTIAELVEQFDLDITKTVKTLIVVGASEATPLVALIVRGD
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHEEECCCCCCCEEEEEEECC
HELNEVKADKLDLVASPVEMAPEALIRDAIGAGPGSLGPIGLNIPIVIDHSVSVMSDFAA
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHC
GANVDDKHYFGINWERDLPLAQVADIRNVVEGEPTPDGSGIYAMARGIEVGHIFQLGTNY
CCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEEECCCC
SKSMNATVLDENGKSQVLLMGCYGVGVSRIVAAAIEQNFDDRGIIWPEAIAPFSVGILPM
CCCCCEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEE
NMHKSHRVTDIAEQLYKDLNEAGIDVLLDDRKERPGVMFADMELIGIPHTVVIGDRNIDA
CCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEECEEEECCCEEEEEECCCCCC
GVFEYKNRRTGEKQDIPFDQLLDFLKNAVKG
CHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA